Invasives: a major conservation threat     

Lambertini M  Leape J  Marton-Lefèvre J  Mittermeier RA  Rose M  Robinson JG  Stuart SN  Waldman B  Genovesi P 《Science (New York, N.Y.)》2011,333(6041):404-405

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通过调整家禽营养改善垫料质量及其生产性能     

肖长峰  Liu Jin  Julia Trautwein  Georg Dusel 《国外畜牧学(猪与禽)》2011,31(1):19-22

家禽湿垫料综合征可造成重大的经济损失。据Butchers和Miles（2009）的研究,垫料质量是家禽生产的关键要素之一。如果垫料质量未被维持在一个可接受的水平上,那么垫料内细菌负荷极高和不卫生的生长条件可能会导致鸡舍内出现异味（包括氨）增加、昆虫滋生（尤其是蝇类）、羽毛脏乱、足垫病变及胸部水泡等问题。  相似文献   

Avian CD154 enhances humoral and cellular immune responses induced by an adenovirus vector-based vaccine in chickens     

Sánchez Ramos O  González Pose A  Gómez-Puerta S  Noda Gomez J  Vega Redondo A  Águila Benites JC  Suárez Amarán L  Parra NC  Toledo Alonso JR 《Comparative immunology, microbiology and infectious diseases》2011,34(3):259-265

Recombinant adenoviral vectors have emerged as an attractive system for veterinary vaccines development. However, for poultry vaccination a very important criterion for an ideal vaccine is its low cost. The objective of this study was to test the ability of chicken CD154 to enhance the immunogenicity of an adenoviral vector-based vaccine against avian influenza virus in order to reduce the amount of antigen required to induce an effective immune response in avian. Chickens were vaccinated with three different doses of adenoviral vectors encoding either HA (AdHA), or HA fused to extracellular domain chicken's CD154 (AdHACD). Hemagglutination inhibition (HI) assay and relative quantification of IFN-γ showed that the adenoviral vector encoding for the chimeric antigen is able to elicit an improved humoral and cellular immune response, which demonstrated that CD154 can be used as a molecular adjuvant allowing to reduce in about 50-fold the amount of adenoviral vector vaccine required to induce an effective immune response.  相似文献   

Transplantation of CD6-depleted peripheral blood stem cells after DLA-haploidentical bone marrow transplantation contributes to engraftment and tolerance in a preclinical model of stem cell transplantation     

Zorn J  Schwamberger S  Panzer W  Adler H  Kolb HJ 《Veterinary immunology and immunopathology》2011,144(1-2):27-35

Human leukocyte antigen (HLA)-haploidentical stem cell transplantation is an opportunity for nearly all patients lacking an HLA matched stem cell donor. However, graft rejection and graft-versus-host disease (GvHD) as well as infectious complications still result in high treatment-related mortality. Here, we used the dog as a preclinical model for the study of tolerance induction with the aim to optimize and to improve a clinical protocol of haploidentical stem cell transplantation. For this purpose CD6-depleted peripheral blood stem cells (PBSCs) were transfused 6d after transplantation of unmodified bone marrow from dog leukocyte antigen (DLA)-haploidentical littermate donors in order to induce immune tolerance. Besides hematopoietic stem cells CD6-depleted PBSC contain, NK cells and a minority of suppressive CD8-positive cells that may suppress activated T lymphocytes. Recipients were conditioned with, cyclophosphamide and antithymocyte globulin (ATG) preceded by a transfusion of donor buffy coat and either 1, 2 or 3 × 3.3 Gy total body irradiation (TBI). Postgrafting immunosuppression was limited to 30 d of cyclosporine and methotrexate. The additional administration of CD6-depleted PBSCs after unmodified marrow could not prevent GvHD, but it may improve engraftment and chimerism after conditioning with 2 × 3.3 Gy TBI. Reasons for incomplete suppression and possible improvements for clinical applications are discussed.  相似文献   

Identification of unique DNA sequences present in highly virulent 2009 Alabama isolates of Aeromonas hydrophila     

Pridgeon JW  Klesius PH  Mu X  Carter D  Fleming K  Xu D  Srivastava K  Reddy G 《Veterinary microbiology》2011,152(1-2):117-125

In 2009, a disease outbreak caused by Aeromonas hydrophila occurred in 48 catfish farms in West Alabama, causing an estimated loss of more than 3 million pounds of food size channel catfish. Virulence studies have revealed that the 2009 isolates of A. hydrophila are at least 200-fold more virulent than a 1998 Alabama isolate AL98-C1B. However, up to now, no molecular markers have been identified to differentiate the highly virulent 2009 isolates from other isolates of A. hydrophila. To understand the genetic differences between the highly virulent 2009 isolates and the less virulent AL98-C1B at molecular level, PCR-select bacterial genome subtractive hybridization was used in this study. A total of 96 clones were selected from the subtractive genomic DNA library. Sequencing results revealed that the 96 clones represented 64 unique A. hydrophila sequences. Of the 64 sequences, three (hypothetical protein XAUC_13870, structural toxin protein RtxA, and putative methyltransferase) were confirmed to be present in the three virulent 2009 Alabama isolates but absent in the less virulent AL98-C1B. Using genomic DNAs from nine field isolates of A. hydrophila with different virulence as templates, two sequences (hypothetical protein XAUC_13870 and putative methyltransferase) were found to be only present in highly virulent A. hydrophila isolates, but absent in avirulent isolates.  相似文献   

Synthesis of a combinatorial library of amides and its evaluation against the fall armyworm, Spodoptera frugiperda     

Castral TC  Matos AP  Monteiro JL  Araujo FM  Bondancia TM  Batista-Pereira LG  Fernandes JB  Vieira PC  da Silva MF  Corrêa AG 《Journal of agricultural and food chemistry》2011,59(9):4822-4827

The fall armyworm Spodoptera frugiperda is a polyphagous pest that causes important damage in different regions of America and mainly affects corn crops in both tropical and subtropical areas. Currently, control relies on both transgenic plants and/or chemical pesticides. In this work we describe the preparation of an indexed combinatorial library of amides and its toxic effect by contact against S. frugiperda . (E)-1-(1-Piperidinyl)-3-[4-(trifluoromethoxy)phenyl]-2-propen-1-one was the most active compound with an LD(50) = 0.793 μg mg(-1) of larva. This amide was also evaluated by ingestion and at the lowest concentration (1 mg kg(-1)) achieved 83.3% mortality.  相似文献   

Lindane Biodegradation by Defined Consortia of Indigenous Streptomyces Strains     

Mar��a Soledad Fuentes  Juliana Mar��a S��ez  Claudia Susana Benimeli  Mar��a Julia Amoroso 《Water, air, and soil pollution》2011,222(1-4):217-231

The current study aimed to compare lindane degradation by pure and mixed cultures of Streptomyces sp. Cell-free extracts were assayed for potentiating dechlorinase activity and, based on these results, consortia of two to six microorganisms were assayed for their growth on and degradation of lindane. Furthermore, the role of bacterial consortia of lindane-degrading strains was examined in lindane decontamination soil assays. Four actinobacteria, previously isolated from a pesticide-contaminated area, were selected because of their tolerance to lindane and their ability to use the pesticide as sole carbon source. These strains as well as Streptomyces sp. M7 and Streptomyces coelicolor A3 were used to study specific dechlorinase activity (SDA) and lindane removal in mixed cultures. Pure cultures presented SDA in the presence of 1.66 mg L^-1 lindane as carbon source. SDA was improved by certain mixed cultures until 12 times compared with pure cultures. Mixed cultures with two, three, and four strains showed maximum lindane removal of 46% to 68%, whereas combinations of five and six strains did not efficiently remove the pesticide from the culture medium. The Streptomyces sp. A2, A5, M7, and A11 consortium presented the lowest ratio between residual lindane concentration and SDA and could be a promising tool for lindane biodegradation.  相似文献   

Effects of metronidazole on the fecal microbiome and metabolome in healthy dogs     

Rachel Pilla  Frederic P. Gaschen  James W. Barr  Erin Olson  Julia Honneffer  Blake C. Guard  Amanda B. Blake  Dean Villanueva  Mohammad R. Khattab  Mustafa K. AlShawaqfeh  Jonathan A. Lidbury  Jörg M. Steiner  Jan S. Suchodolski 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(5):1853-1866

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Efficacy of adding ramipril (VAsotop) to the combination of furosemide (Lasix) and pimobendan (VEtmedin) in dogs with mitral valve degeneration: The VALVE trial     

Gerhard Wess  Jan-Gerd Kresken  Ralph Wendt  Juliane Gaugele  Markus Killich  Lisa Keller  Julia Simak  Peter Holler  Alexander Bauer  Helmut Küchenhof  Tony Glaus 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(6):2232-2241

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Long-term postoperative pain evaluation in dogs with thoracolumbar intervertebral disk herniation after hemilaminectomy     

Natalia Zidan  Julia Medland  Natasha Olby 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(4):1547-1555

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