Hemostatic defects associated with two infusion rates of dextran 70 in dogs.     

K T Concannon  S C Haskins  B F Feldman 《American journal of veterinary research》1992,53(8):1369-1375

We investigated changes in hemostatic function after infusion of 6% dextran 70 (high molecular weight dextran) at 2 rates. Six healthy dogs underwent 3 regimens: 20 ml of dextran/kg of body weight administered in 1 hour (trial A), 20 ml of dextran/kg administered in 30 minutes (trial B), and 0.9% sodium chloride solution as a control administered over 1 hour to achieve hemodilution equivalent to that for 20 ml of dextran/kg (trial C). Before and at 2, 4, 8, and 24 hours after the start of trials A and B, we measured PCV, total solids (TS) concentration, amount of von Willebrand factor antigen (vWf:Ag), factor VIII coagulant activity (VIII:C), prothrombin time, activated partial thromboplastin time (APTT), platelet retention in a glass bead column, and buccal mucosa bleeding time (BMBT). Values were not obtained at 8 and 24 hours for trial C. Saline-induced changes in hemostasis were significant (P less than 0.05) from baseline throughout the sample collection period. Significant differences (P less than 0.05) between trial A and control were observed for vWf:Ag, VIII:C, BMBT, APTT, TS, and PCV values at 2 hours, and for VIII:C at 4 hours. Significant differences (P less than 0.05) between trial B and control were observed for APTT, TS, and PCV values at 2 hours, and for vWf:Ag, VIII:C, BMBT, APTT, TS, and PCV values at 4 hours. During trials A and B, mean values of analytes infrequently deviated from reference intervals, and clinical signs of bleeding were not observed in any dog.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Effects of 'Agri-SC' soil conditioner on soil structure and erodibility: some further observations     

M.A. Fullen  A.M. Tye  K.E. Cookson 《Soil Use and Management》1995,11(4):183-185

Abstract. In field and laboratory experiments the conditioner‘Agri-SC’has shown improvements in the structure of loamy sand soils in east Shropshire, UK. It resulted in statistically significant decreases in soil bulk density values and increases in soil porosity and aggregate stability. Further experiments are in progress on both loamy sand and silt loam soils.  相似文献   

咖啡黑小蠹的发生规律及药剂防治研究   总被引：1，自引：0，他引：1  

林延谋  章创鹏 《热带作物学报》1994,15(2):79-86

咖啡黑小蠢是近年在兴隆华侨农场严重危害中粒种咖啡的重要害虫。据1989年对4—5年生咖啡树调查,植株受害率100％,枝条受害率29.7—82.5％,虫害枯枝率7.65—17.8％。该虫以雌成虫钻蛀咖啡枝条为害,幼虫和成虫取食蛀道壁上的真菌菌丝。田间种群数量通常在3月上旬开始剧增,3月中下旬为高峰期,7月至10月田间虫口极少,11月以后逐渐有虫口及虫枯枝出现。温度是影响虫口波动的主要因素。2.5％溴氰菊酶、25％杀虫双、40％乐菊酯各兑水1000倍对成虫直接触杀效果显著,死亡率均为100％。48％乐斯本、40％氧化乐果400—800倍液等12种杀虫剂田间喷雾对咖啡枝条蛀洞内的成虫、蛹、幼虫防效均不明显。  相似文献   

Protection of chickens from Newcastle and Marek's diseases with a recombinant herpesvirus of turkeys vaccine expressing the Newcastle disease virus fusion protein.   总被引：5，自引：0，他引：5  

R W Morgan  J Gelb  C S Schreurs  D Lütticken  J K Rosenberger  P J Sondermeijer 《Avian diseases》1992,36(4):858-870

Recombinant strains of herpesvirus of turkeys (HVT) were constructed that contain either the fusion protein gene or the hemagglutinin-neuraminidase gene of Newcastle disease virus (NDV) inserted into a nonessential gene of HVT. Expression of the NDV antigens was regulated from a strong promoter element derived from the Rous sarcoma virus long terminal repeat. Recombinant HVT strains were stable and fully infectious in cell culture and in chickens. Chickens receiving a single intra-abdominal inoculation at 1 day of age with recombinant HVT expressing the NDV fusion protein had an immunological response and were protected (> 90%) against lethal intramuscular challenge at 28 days of age with the neurotropic velogenic NDV strain Texas GB. Recombinant HVT expressing the NDV hemagglutinin-neuraminidase provided partial protection (47%) against the same challenge. Chickens vaccinated with recombinant HVT vaccines had low levels of protection against NDV replication in the trachea when challenged ocularly. Recombinant HVT vaccines and the parent HVT strain provided similar levels of protection to chickens challenged with the very virulent RB1B strain of Marek's disease virus, indicating that insertion of foreign sequences into the HVT genome did not compromise the ability of HVT to protect against Marek's disease.  相似文献   

野大麦人工栽培驯化试验   总被引：1，自引：0，他引：1  

董卫民  李阜憬 《草业科学》1992,9(5):38-39

  相似文献   

Effect of prostaglandin F2 alpha on adrenal-produced steroid hormones in cows     

E D Watson  K Hinrichs 《American journal of veterinary research》1989,50(7):1078-1079

Ovariectomized, nonlactating cows were treated with IM injections of either physiologic saline solution or prostaglandin F2 alpha. Plasma concentrations of cortisol increased significantly by 30 to 60 minutes after injection of prostaglandin F2 alpha, but there were no significant increases in plasma concentrations of estradiol, progesterone, or testosterone. After saline solution treatment, there were no increases in any of the hormones measured.  相似文献   

湖南省果梅品种资源种质杂化状况的初步研究   总被引：8，自引：0，他引：8  

刘连森  贺善文 《园艺学报》1993,20(3):225-230

  相似文献   

水平连续铸钢凝固过程数值模拟     

刘金水  蒋冰 《湖南农业大学学报(自然科学版)》1995,22(3)

本文在分析水平连铸传热特征的基础上，给出了一维情况下，计算结晶器出口处铸坯凝固层厚度的解析式，建立了计及轴向传热的连续坯凝固模拟模型。进行了连续坯二维传热的凝固数值模拟，其模拟结果与实验结果吻合较好，具有普遍应用价值。  相似文献   

仙客来离体叶培养与植株再生   总被引：8，自引：0，他引：8  

张喜春  刘宏伟 《东北林业大学学报》1992,20(4):18-21

以仙客来叶片、叶柄及其不同部位为外植体进行诱导,结果幼叶基部(叶子基部与叶柄相连处的断口部分)诱导愈伤组织频率最高,可达100％。愈伤组织在N_6+BA2mg/1+2,4-D0.5mg/1培养基上培养,约40d左右可100％分化出不定芽,其不定芽数量一块愈伤组织最多可分化出30个以上。将幼苗移入1/2MS+NAA 0.5mg/1培养基中生根最好。  相似文献   

Release of tumor necrosis factor-alpha from bovine alveolar macrophages stimulated with bovine respiratory viruses and bacterial endotoxins     

S.E. Bienhoff  G.K. Allen  J.N. Berg 《Veterinary immunology and immunopathology》1992,30(4):341-357

The release of tumor necrosis factor-alpha (TNF-) from cultured bovine alveolar macrophages (BAM) was evaluated following stimulation of BAM with bovine herpesvirus-1 (BHV-1), parainfluenza-3 (PI-3) virus, bovine respiratory syncytial virus (BRSV), Escherichia coli 0111:B4 endotoxin, Pasteurella haemolytica type 1 endotoxin, Pasteurella multocida endotoxin, and virus/endotoxin combinations. A cytotoxic assay system using Georgia bovine kidney cells as targets was used to measure TNF- activity. The cytotoxic activity was neutralized by an anti-human TNF- monoclonal antibody.

Stimulation of BAM with 1 median tissue culture infectious dose (TCID₅₀) of live or ultraviolet (UV)-inactivated PI-3 virus/cell resulted in release of TNF- in significantly (P<0.05) higher amounts than sham-induced BAM. The quantities of TNF- released after live or UV-inactivated BHV-1 or BRSV induction were not significantly higher than sham-induced BAM. E. coli 0111:B4, P. haemolytica type 1 and P. multocida endotoxins stimulated TNF- release in a dose-dependent manner. Sequential exposure of BAM to 1 TCID₅₀ per cell of either live BHV-1, PI-3 virus or BRSV and then 5 μg ml⁻¹ of either E. coli 0111:B4, P. haemolytica type 1 or P. multocida endotoxin caused a significant (P<0.05) reduction in detectable TNF- in seven of nine virus/endotoxin combinations tested, when compared with 5 μg ml⁻¹ of endotoxin alone. Parainfluenza-3 virus/endotoxin combinations stimulated higher TNF- release when compared with other virus/endotoxin combinations. Five out of six test animals had serum-neutralizing antibodies to PI-3 virus, one out of six had serum-neutralizing antibodies to BHV-1, and two out of six had serum-neutralizing antibodies to BRSV, suggesting a possible relationship between serum neutralizing antibodies and TNF- release from in vitro cultivated BAM.  相似文献