鹅MTTP基因多态性与脂质指标和屠体性状的相关研究     

王浩瀚  胡深强  寇洁  敬准  杨宇  余国春  康波  王继文 《中国家禽》2010,32(12)

利用PCR-SSCP技术检测四川白鹅(n=24)和朗德鹅(n=33)MTTP基因的第8、18和21外显子的多态性,计算基因频率和基因型频率,并将发现的多态性与鹅肝脂质指标和屠体性状进行关联分析,研究MTTP基因多态性与鹅肝脂质指标和屠体性状的相关关系。结果发现,鹅MTTP基因第21外显子130 bp处存在G→A突变,该突变位点在朗德鹅群体中表现出AA和BB 2种基因型,AA基因型鹅的肝脂质指标和屠体性状与BB基因型个体差异不显著(P0.05);在四川白鹅群体中仅存在BB基因型,鹅MTTP基因的该突变位点对鹅肝脂质指标和屠体性状的影响不显著。  相似文献   

中药"增免散"对肉鸡免疫器官及生长发育的影响   总被引：2，自引：0，他引：2  

黄军  朱红旗  吕建存  林敏  田森 《中国兽药杂志》2006,40(10):23-26

1日龄艾维茵肉鸡健雏200只随机分4组，“增免散”按饲料量0．5％、1％、1．5％分别添加到Ⅰ、Ⅱ、Ⅲ组，对照组为Ⅳ组。Ⅰ、Ⅱ、Ⅲ组从第7天开始投药，各组于第7、21天用LaSota活苗免疫，然后于第7、14、28、49天测定血液中HI抗体水平，并检测“增免散”对免疫器官和生长发育的影响。结果表明，中药“增免散”按1％、1．5％添加能显著提高Lasota活苗接种雏鸡的HI抗体水平，延长其持续时间，能促进雏鸡免疫器官的生长发育，并能促进肉鸡的生长发育，提高饲料转化率。  相似文献   

藿香植物组织培养技术研究     

李红  李永文  寇凤仙  周彦珍  温秀荣 《北方园艺》2007,(9):195-197

选用藿香健壮枝条和叶片作为试验材料,进行组培技术的研究.结果表明:藿香取材季节以夏季较好,适宜的外植体种类为茎段.诱导愈伤组织形成的激素组合为6-BA 1.0mg/L NAA 0.3 mg/L 2,4-D 0.3 mg/L,具有愈伤组织生长速度快并易于分化;不定芽分化激素组合为6-BA 0.5 mg/L NAA 0.1 mg/L,继代培养组合6-BA 0.1 mg/L NAA 0.5 mg/L B90.3mg/L,增殖在8倍以上;生根培养组合1/2MS NAA0.6 mg/L,一般20 d左右生根,平均根量4～5条,生根率达92%.  相似文献   

西瓜播种前种子处理概述   总被引：2，自引：1，他引：2  

张龑  寇明明  孙小武 《中国瓜菜》2007,(4):29-31

西瓜是栽培面积较大的作物之一。西瓜种子处理对其幼苗的活力、发芽率以及西瓜生产均有很大的影响。文章概述了西瓜种子播种前的处理方法及其作用,对今后西瓜播种前种子处理的发展趋势进行了展望。  相似文献   

油松母树林经营技术研究   总被引：1，自引：0，他引：1  

ZHU Xiao-qiang  寇友民 《辽宁林业科技》2008,(4)

为提高油松种子产量与质量,进行油松母树林密度、坡向、土壤耕作施肥等试验,结果表明:密度以450～675株/hm2为宜,阳坡为好,土壤耕作施肥、树冠下地表覆盖均比未耕作施肥和地表不覆盖的油松母树种子结实多,质量好.为提高油松母树林种子产量和质量,经营好母树林提供科学依据.  相似文献   

益生菌发酵乳无菌提取物对空肠弯曲杆菌flaA σ²⁸启动子的抑制作用（英文）     

丁武寇莉萍  王海风胡巅 《西北农林科技大学学报(自然科学版)》2007,35(10):173-177,182

为了研究益生菌发酵乳无菌提取物对食源性有害微生物(空肠弯曲杆菌)有毒基因表达的抑制作用,以利用空肠弯曲杆菌的有毒基因flaA σ28启动子和无启动子的质粒pRYluxCDABE联结构建的生物冷光转基因模型为试验材料,通过检测生物发光特性的方法,研究了空肠弯曲杆菌有毒基因flaA表达与益生菌(5种双岐杆菌,6种乳酸杆菌)发酵乳无菌提取物之间的关系。结果表明,益生菌发酵乳无菌提取物对空肠弯曲杆菌flaA σ28启动子的活性有显著的抑制作用(P<0.05),并可显著抑制空肠弯曲杆菌有害基因flaA的表达。  相似文献   

高寒草甸植物根际溶磷菌的筛选鉴定及其溶磷与促生效果     

蔺宝珺  杨文权  赵帅  柴港宁  鱼杨华  武燕茹  韩显忠  李希来  寇建村 《草地学报》2022,30(11):3132-3139

为获得高寒草甸根际土中优良溶磷菌资源,本研究从青海省高寒草甸根际土中筛选了4株溶磷菌,结合16S rRNA基因分析法确定其分类地位,并通过钼锑抗比色法和盆栽试验进行了溶磷与促生效果的研究。结果显示：4株溶磷菌均为假单胞菌属(Pseudomonas sp.),均可形成明显的溶磷圈。在无机磷液体培养基中培养7d后,4株菌株的磷增量在156.17~511.33μg·mL^-1之间;溶磷过程中4株菌均分泌多种有机酸,总有机酸量在522.36~986.69mg·L^-1之间;盆栽试验表明4株菌均能显著增加披碱草(Elymus dahuricus Turcz.)株高和地上部干重;菌株MXSC5,MXSC6和MQC13可使植株全氮、全磷含量增加,且4株菌株对土壤速效氮、速效磷含量也有正向影响。本研究结果将有助于进一步探究溶磷菌对植物的促生作用及微生物菌肥的开发利用。  相似文献   

紫肉甘薯及其突变体花青素积累差异的比较转录组分析     

后猛  李臣  宋炜涵  张允刚  李强 《江苏农业学报》2022,38(2):313-325

紫肉甘薯因其块根中富含紫色花青素而得名.本研究以2组栽培种紫肉甘薯及其淡黄肉突变体为材料,基于甘薯转录组测序(RNA-seq)数据进行生物信息学分析.以|log2 FC|≥1且错误发现率(FDR)<0.01作为筛选标准,鉴定差异表达基因(DEGs),对DEGs进行GO功能分类和KEGG代谢通路分析,并采用实时荧光定量P...  相似文献   

Partial organic substitution weakens the negative effect of chemical fertilizer on soil micro-food webs          下载免费PDF全文

LIU Han-wen  ZHANG Xiao-ke  ZHANG Gui-zong  KOU Xin-chang  LIANG Wen-ju 《农业科学学报》2022,21(10):3037-3050

Soil biotic communities play vital roles in enhancing soil nutrient cycling and soil fertility. Long-term excessive nitrogen (N) application is disadvantageous to the stability of soil food webs and affects arable soil health and sustainable utilization. Proper organic substitution is essential to improve soil health and alleviate the disadvantages of excessive chemical fertilization. However, the biological effects of various organic amendments on soil micro-food webs are poorly understood. In order to explore the effects of various organic amendments including stover, biochar and manure on soil micro-food webs (microbial and nematode communities), a field plot experiment with maize having five treatments viz., 100% urea (100% N), 70% urea (70% N), 70% urea plus stover (Stover), 70% urea plus cattle manure (Manure) and 70% urea plus biochar (Biochar) was conducted. Manure treatment increased the carbon (C) to N use efficiency of soil microbes, which contributed to the retention of soil C, while Biochar treatment elevated soil organic C (SOC) and soil pH. Additionally, Biochar treatment mitigated the negative effects of soil acidification on the soil micro-food web and reduced the abundance of plant parasites. Overall, the biological effect of organic amendments was distinguished from chemical fertilization (100% N and 70% N) through principal co-ordinates analysis. Negative relationships among soil properties, microbial and nematode biomass in the 100% N treatment were diminished in treatments where chemical fertilizer was reduced. The bottom-up effects on soil food webs were observed in organic substitution treatments. In conclusion, organic amendments improved soil fertility by regulating soil microbial and nematode communities in the cropland ecosystem, alleviated the negative effects of chemical fertilizer on the micro-food webs and controlled the trophic cascades among soil biota.  相似文献   

Detection of Penaeus monodon-type baculovirus (MBV) infection in Penaeus monodon Fabricius by in situ hybridization   总被引：2，自引：0，他引：2  

C. C. LU  K. F. J. TANG  G. H. KOU  S. N. CHEN 《Journal of fish diseases》1995,18(4):337-345

Abstract. A digoxigenin-labelled DNA probe was used for in situ detection of the Penaeus monodon -type baculovirus (MBV) derived from cloned MBV polyhedrin genome in cultured Penaeus monodon Fabricius. First, the specificity of the probe against MBV DNA with dot blot hybridization analysis was verified. This probe indicated that cloned MBV polyhedrin fragment can be used as an MBV-specific probe. This was then used to microscopically examine sections of MBV-infected tissues for a blue-purple precipitate indicative of a positive reaction for MBV. MBV-positive cells were located only in the epithelium of the hepatopancreatic tubules and of the midgut. Furthermore, comparison of the susceptibility to MBV infection among several life-stages of the shrimp showed that the MBV genome was found in the zoea, mysis, post-larva, and adult stages, whereas MBV DNA was not detected in either eggs or nauplii. The results were quantified from in situ hybridization with an image analyser to compare the degree of cell infection among groups of cultured P. monodon collected from various farms in Taiwan.  相似文献