紫肉甘薯及其突变体花青素积累差异的比较转录组分析     

后猛  李臣  宋炜涵  张允刚  李强 《江苏农业学报》2022,38(2):313-325

紫肉甘薯因其块根中富含紫色花青素而得名.本研究以2组栽培种紫肉甘薯及其淡黄肉突变体为材料,基于甘薯转录组测序(RNA-seq)数据进行生物信息学分析.以|log2 FC|≥1且错误发现率(FDR)<0.01作为筛选标准,鉴定差异表达基因(DEGs),对DEGs进行GO功能分类和KEGG代谢通路分析,并采用实时荧光定量P...  相似文献   

Partial organic substitution weakens the negative effect of chemical fertilizer on soil micro-food webs          下载免费PDF全文

LIU Han-wen  ZHANG Xiao-ke  ZHANG Gui-zong  KOU Xin-chang  LIANG Wen-ju 《农业科学学报》2022,21(10):3037-3050

Soil biotic communities play vital roles in enhancing soil nutrient cycling and soil fertility. Long-term excessive nitrogen (N) application is disadvantageous to the stability of soil food webs and affects arable soil health and sustainable utilization. Proper organic substitution is essential to improve soil health and alleviate the disadvantages of excessive chemical fertilization. However, the biological effects of various organic amendments on soil micro-food webs are poorly understood. In order to explore the effects of various organic amendments including stover, biochar and manure on soil micro-food webs (microbial and nematode communities), a field plot experiment with maize having five treatments viz., 100% urea (100% N), 70% urea (70% N), 70% urea plus stover (Stover), 70% urea plus cattle manure (Manure) and 70% urea plus biochar (Biochar) was conducted. Manure treatment increased the carbon (C) to N use efficiency of soil microbes, which contributed to the retention of soil C, while Biochar treatment elevated soil organic C (SOC) and soil pH. Additionally, Biochar treatment mitigated the negative effects of soil acidification on the soil micro-food web and reduced the abundance of plant parasites. Overall, the biological effect of organic amendments was distinguished from chemical fertilization (100% N and 70% N) through principal co-ordinates analysis. Negative relationships among soil properties, microbial and nematode biomass in the 100% N treatment were diminished in treatments where chemical fertilizer was reduced. The bottom-up effects on soil food webs were observed in organic substitution treatments. In conclusion, organic amendments improved soil fertility by regulating soil microbial and nematode communities in the cropland ecosystem, alleviated the negative effects of chemical fertilizer on the micro-food webs and controlled the trophic cascades among soil biota.  相似文献   

Detection of Penaeus monodon-type baculovirus (MBV) infection in Penaeus monodon Fabricius by in situ hybridization   总被引：2，自引：0，他引：2  

C. C. LU  K. F. J. TANG  G. H. KOU  S. N. CHEN 《Journal of fish diseases》1995,18(4):337-345

Abstract. A digoxigenin-labelled DNA probe was used for in situ detection of the Penaeus monodon -type baculovirus (MBV) derived from cloned MBV polyhedrin genome in cultured Penaeus monodon Fabricius. First, the specificity of the probe against MBV DNA with dot blot hybridization analysis was verified. This probe indicated that cloned MBV polyhedrin fragment can be used as an MBV-specific probe. This was then used to microscopically examine sections of MBV-infected tissues for a blue-purple precipitate indicative of a positive reaction for MBV. MBV-positive cells were located only in the epithelium of the hepatopancreatic tubules and of the midgut. Furthermore, comparison of the susceptibility to MBV infection among several life-stages of the shrimp showed that the MBV genome was found in the zoea, mysis, post-larva, and adult stages, whereas MBV DNA was not detected in either eggs or nauplii. The results were quantified from in situ hybridization with an image analyser to compare the degree of cell infection among groups of cultured P. monodon collected from various farms in Taiwan.  相似文献   

北京市怀柔区生态用水计算研究     

张东  贺康宁  寇中泰  张益源 《水土保持研究》2010,17(1)

根据生态平衡理论,依据北京市怀柔区的实际情况,划分了北京市怀柔区生态用水类型,并将怀柔区划分为相互独立的二级生态区;计算出怀柔区各项生态用水量。结果为:全区现状生态用水总量为7.19亿m3/a。其中,森林植被生态用水量为4.65亿m3/a,水土保持生态用水量为0.83亿m3/a,城镇绿化生态用水量为0.02亿m3/a,草地生态用水量为1.29亿m3/a,河流生态系统生态用水为0.4亿m3/a。  相似文献   

支持向量机、信息向量机和相关向量机的比较研究     

寇玉香 《计算机与农业》2010,(7):149-153

介绍了支持向量机、信息向量机和相关向量机的理论与算法。利用最优化对偶理论,阐述了支持向量机的三种主要算法：硬间隔支持向量机、软间隔线性支持向量机和二次软间隔支持向量机的理论推导过程。对基于高斯过程模型,详细说明了信息向量机和相关向量机算法的实现过程。  相似文献   

小麦TaNCED1基因植物表达载体的构建   总被引：1，自引：1，他引：0  

西廷业  王洪刚  后猛  刘海燕  刘树兵 《华北农学报》2008,23(4)

以植物双元表达载体pCAMBIA2300-35为基础,设计带有酶切位点Kpn I和Xba I的一对引物,从克隆载体pGEM-NCEDI扩增到目的基因TaNCED1,酶切回收后与同样双酶切的表达载体pCAMBIA2300-35连接,获得表达载体pTaNCED!,并将所构建的载体导入根癌农杆菌GV3101菌株,为该基因的功能鉴定及通过基因工程的方法提高小麦的抗逆性奠定了基础.  相似文献   

1-甲基环丙烯处理对不同成熟度薄皮甜瓜贮藏特性的影响   总被引：2，自引：0，他引：2  

侯田莹  宋曙辉  郑淑芳  寇文丽  李武 《中国瓜菜》2012,(2):6-10

以京蜜薄皮甜瓜为试材,采用体积浓度2μL·L-1的1-甲基环丙烯(1-MCP)分别处理八成熟和九成熟薄皮甜瓜,随后置于15℃冷库中贮藏,贮藏期间每3d测定相关生理和品质指标变化。结果表明:成熟度对薄皮甜瓜贮藏特性有显著影响,八成熟比九成熟薄皮甜瓜耐贮;1-MCP处理可以显著抑制不同成熟度薄皮甜瓜呼吸强度、乙烯释放速率,从而减少其贮藏期间质量损失,延缓果实表皮颜色转黄和后熟过程,降低贮藏期间腐烂率,保持其食用品质和口感,延长薄皮甜瓜货架期;八成熟的1-MCP处理组贮藏效果最好。  相似文献   

中国沿海半干旱地区海水养殖废水灌溉耐盐植物(菊芋)的研究     

ZHAO Geng-Mao  LIU Zhao-Pu  CHEN Ming-D  KOU Wei-Feng 《土壤圈》2006,16(6):762-769

An experiment with six treatments： CK1 （rainfed）, CK2 （irrigated with freshwater）, and 4 treatments of saline aquaculture effluent blended with brackish groundwater at different ratios of 1：1, 1：2, 1：3, and 1：4 （v/v） was carried out during 2004 to assess the effect of saline aquaculture effluent on plant growth and soil properties in the Laizhou region, Shandong Province, China and to determine an optimal salinity threshold for aquaculture effluent. Cumulative evapotranspiration for the saline aquaculture effluent irrigation and non-irrigation treatments was lower than that for the freshwater irrigation treatment. Soil electrical conductivity was higher with respect to saline aquaculture effluent irrigation treatment compared to that with respect to non-irrigation or freshwater irrigation treatment. For Jerusalem artichoke （Helianthus tuberosus L.）, in comparison to the freshwater treatment, plant height and aboveground biomass for the 1：3 and 1：4 treatments were constrained, whereas stem width and root biomass were enhanced. Concomitantly, higher tuber yield was obtained for the 1：3 and 1：4 treatments compared to that for CK1 and 1：1 treatments. Nitrogen and phosphorus were higher in tubers of the 1：4 treatment. This study demonstrated that saline aquaculture effluent could be used successfully to irrigate Jerusalem artichoke with higher tuber yield and nutrient removal.  相似文献   

基于GIS与137Cs技术的土壤侵蚀微观研究   总被引：3，自引：0，他引：3  

吴永红  张虎林  寇权  孙耀胜  刘海燕 《水土保持研究》2007,14(2):40-42

坡地侵蚀的研究是进行流域侵蚀研究的基础，影响坡地土壤侵蚀的因素很多，但最主要的是地形因子。通过^137Cs技术和GIS技术，以黄土高塬沟壑区的多个自然汇流坡面为研究对象，从微观角度探讨了在自然坡面条件下不同地形因子对坡面土壤侵蚀的影响，根据土壤侵蚀在坡面上的垂直分异规律．对不同部位土壤侵蚀量的差异及不同地形因子对土壤侵蚀的影响大小进行了初步探索性研究。结果表明：在微观尺度内，坡度、坡长等常规因子对土壤侵蚀的影响并不像径流小区测定的那样明显，似乎已经退居到次要位置。借助GIS对土壤侵蚀进行微观研究，可以发现一些用常规方法很难发现的规律，有助于我们更加深刻地认识土壤侵蚀的变化规律。  相似文献   

黄芩在承德县的种子繁殖和高产栽培技术     

寇永猛 《种子世界》2011,(5):40-41

黄芩又名山茶根、黄芩茶。属唇形科,多年生草本植物。一般黄芩株高30~110cm,主根粗壮,略呈圆锥形,棕褐色。断面金黄色。茎枝四棱形,单生或簇生,茎上多分枝,直立或半直立。叶片单叶对生,形状披针形,总状花序。顶生花偏向一侧,花冠蓝紫色,个别也有白色、浅白色。果实较小,坚果、肾形,黑褐色。  相似文献