Molecular Typing and Presence of Genetic Markers Among Strains of Banana Finger-Tip Rot Pathogen, Burkholderia cenocepacia, in Taiwan     

Lee YA  Chan CW 《Phytopathology》2007,97(2):195-201

ABSTRACT Burkholderia cenocepacia (genomovar III of B. cepacia complex), the causal agent of banana finger-tip rot, is a common plant-associated bacterium but also an important opportunistic pathogen of humans. To better understand the nature of B. cenocepacia from banana, the genetic variation among B. cenocepacia isolates from various banana-growing regions in southern Taiwan was examined. Forty-four serial isolates recovered from diseased banana stigmata from three banana-growing regions during the periods ranging from 2002 to 2004 were investigated. All B. cenocepacia isolates picked from quinate-yeast extract tetracycline-polymyxin semiselective medium could cause onion maceration and were polymerase chain reaction (PCR) positive for bcscV, which is a type III secretion gene present in all members of the B. cepacia complex except B. cepacia (formerly genomovar I). Genetic diversity was assessed using recA PCR restriction fragment length polymorphism, recA nucleotide sequence analysis, and pulsed-field gel electrophoresis assays. The assays revealed the genetic variability among the isolates and also allowed us to trace the relationship among isolates. The isolates all were assigned to genomovar III and consisted of two groups, A and B, which corresponded to recA lineage IIIA and IIIB. The group B strains were separated into B1 and B2 subgroups and the B1 strains were further divided into distinct lineages. The B1 strains were the most frequently detected and occurred in all regions tested. There was no significant difference between strains from each subgroup in the virulence on banana fingers of cv. Cavendish. PCR assays were further used to determine whether B. cenocepacia from banana contained the cable pilus subunit gene (cblA), IS1356, and B. cepacia epidemic strain marker (BCESM), which are DNA markers associated with epidemic B. cepacia clinic strains. The results indicated that cblA and IS1356 were absent but the BCESM was found in all isolates. The present study revealed that banana is a natural reservoir of genetically diversified B. cenocepacia strains.  相似文献   

Comparison of computed tomography pulmonary angiography and point‐of‐care tests for pulmonary thromboembolism diagnosis in dogs     

D. L. Chan  L. Benigni  L. Kellett‐Gregory  V. L. Fuentes 《The Journal of small animal practice》2014,55(4):190-197

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A new Brucella canis species-specific PCR assay for the diagnosis of canine brucellosis     

Sung-Il Kang  Sang-Eun Lee  Ji-Yeon Kim  Kichan Lee  Jong-Wan Kim  Hyang-Keun Lee  So-Ra Sung  Young-Ran Heo  Suk Chan Jung  Moon Her 《Comparative immunology, microbiology and infectious diseases》2014

Brucellosis is a zoonotic disease that is transmitted from animals to humans, and the development of a rapid, accurate, and widely available identification method is essential for diagnosing this disease. In this study, we developed a new Brucella canis species-specific (BcSS) PCR assay and evaluated its specificity and sensitivity. A specific PCR primer set was designed based on the BCAN_B0548-0549 region in chromosome II of B. canis. The PCR detection for B. canis included amplification of a 300-bp product that is, not found on other Brucella species or, genetically or serologically related bacteria. The detection limit of BcSS-PCR assay was 6 pg/μl by DNA dilution, or 3 × 10³ colony-forming units (CFU) in the buffy coats separated from whole blood experimentally inoculated with B. canis. Using the buffy coat in this PCR assay resulted in approximately 100-times higher sensitivity for B. canis as compared to detect directly from whole blood. This is the first report of a species-specific PCR assay to detect B. canis, and the new assay will provide a valuable tool for the diagnosis of B. canis infection.  相似文献   

The siderophore-interacting protein is involved in iron acquisition and virulence of Riemerella anatipestifer strain CH3     

Jing Tu  Fengying Lu  Shuang Miao  Xintao Ni  Pan Jiang  Hui Yu  Linlin Xing  Shengqing Yu  Chan Ding  Qinghai Hu 《Veterinary microbiology》2014,168(2-4):395-402

Riemerella anatipestifer causes epizootic infectious disease in poultry and serious economic losses especially to the duck industry. However, little is known regarding the molecular basis of its pathogenesis. The ability to acquire iron under low-iron conditions is related to the virulence of a variety of bacterial pathogens. In this study, a sip (Riean_1281) deletion mutant CH3Δsip was constructed and characterized for iron-limited growth, biofilm formation, and pathogenicity to ducklings. Results showed that siderophore-interacting protein (SIP) was involved in iron utilization and the sip deletion significantly reduced biofilm formation and adherence to and invasion of Vero cells. In addition, the sip gene was absent in 1 of 24 (4.17%) virulent strains and 2 of 3 (66.7%) avirulent strains of R. anatipestifer, and the sip gene from six R. anatipestifer strains, which belong to serotypes 1, 2, and 10, respectively, shared 100% amino acid identities to those of R. anatipestifer strains DSM15868 and RA-GD. These results suggested that siderophore-mediated iron acquisition may be an important iron-uptake pathway in R. anatipestifer. Animal experiments indicated that the median lethal dose of the CH3Δsip mutant in ducklings was about 35-fold higher than that of the wild-type CH3 strain. Thus, our results demonstrated that R. anatipestifer SIP was involved in iron acquisition and necessary for its optimal virulence.  相似文献   

Expression of Perilipin 2 (PLIN2) in Porcine Oocytes During Maturation     

RN Zhang  XW Fu  BY Jia  C Liu  KR Cheng  SE Zhu 《Reproduction in domestic animals》2014,49(5):875-880

Perilipins have been reported to limit the interaction of lipases with neutral lipids within the droplets, thereby regulating neutral lipid accumulation and utilization. This study aimed to identify the location and expression of PLIN1 and PLIN2 in porcine oocytes during maturation. Quantitative real‐time polymerase chain reaction (qRT‐PCR), immunostaining and Western blot methods were used to characterize the expression and distribution patterns of PLIN1 and PLIN2 in porcine oocytes. The results showed that PLIN1 was not detectable in porcine oocytes. PLIN2 and BODIPY 493/503‐detected neutral lipid droplets appeared identical distribution patterns and extensive colocalization in both GV and MII porcine oocytes. PLIN2 protein expression was higher in GV oocytes than that in MII oocytes (p < 0.05), although PLIN2 mRNA expression was similar in both groups. These findings suggested that PLIN2 was a major lipid droplet‐associated protein in porcine oocytes.  相似文献   

Distribution of free L-cysteine and glutathione in seaweeds   总被引：1，自引：0，他引：1  

Makoto Kakinuma  Chan Sun Park  Hideomi Amano 《Fisheries Science》2001,67(1):194-196

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淀山湖鱼类群落结构多样性的年际变化   总被引：2，自引：2，他引：0  

韩婵  高春霞  田思泉  戴小杰 《上海海洋大学学报》2014,23(3):403-410

淀山湖是上海市内陆水域最大的淡水湖泊,主要的淡水渔业水域和水产品来源地,也是重要的水生生物保护基地。为了评估增殖放流和生态环境变化对淀山湖鱼类群落结构变化的影响,本研究以2010-2012年淀山湖的渔业调查资料为基础,对该湖泊的鱼类优势种组成和多样性指数进行年际变化分析,并应用丰度生物量曲线方法对该湖的鱼类群落状况进行分析。结果表明:2010-2012年淀山湖鱼种类数基本稳定,基本鱼种组成变化不显著,优势鱼种组成趋势渐以小型鱼类为主;群落结构多样性指数年间差异不显著(P0.05);ABC曲线显示2010-2012这三年淀山湖群落的数量优势度曲线均高于生物量的优势度曲线,鱼类群落结构仍处于严重干扰状态。因此,建议改善增殖放流鱼种和加强渔业管理,以维持淀山湖鱼类群落的稳定。  相似文献   

东风11 G型内燃机车牵引缓冲装置关键部件检修工艺     

单艳芬 《林业机械与木工设备》2014,(8):35-37

介绍了东风11 G型内燃机车牵引缓冲装置的作用及主要结构,并以其关键部件车钩、缓冲器为研究对象,研究其检修工艺。  相似文献   

Ciguatoxin in moray eels raising the risk for seafood safety in Viet Nam     

Dao  Ha Viet  Le  Hy Ho Khanh  Le  Thao Thi Thu  Pham  Ky Xuan  Bui  Minh Quang  Chan  Leo Lai 《Fisheries Science》2022,88(6):821-830

Fisheries Science - Recently, suspected ciguatera fish poisoning (CFP) cases caused by the consumption of moray eels, popular seafood for locals and tourists, have been reported in Viet Nam....  相似文献   

奶牛分子育种专题学习网站的建设构想     

谢群  熊婵 《计算机与农业》2010,(2):114-115

阐述了建设奶牛分子育种专题学习网站的意义和构想,规划了网站的主要内容和发展方向,为相关专业的研究者和从业人员提供参考。  相似文献