Cloning and sequencing of the ovine gamma-interferon gene   总被引：1，自引：0，他引：1  

AJ RADFORD  ALM HODGSON  JS ROTHEL  PR WOOD 《Australian veterinary journal》1991,68(3):82-84

Cytokines are major modulators of the immune system of all animals. The cloning and expression of recombinant cytokine genes have permitted the analysis of their immune function and role in the control of the immune response to disease and vaccination. While human, murine, and bovine genes have been cloned and sequenced, the cloning of ovine cytokine genes has not yet been reported. As sheep are of dominant economic importance to the Australian farming industry, it is of significance to clone and express these genes to facilitate the development of new and better vaccines and pharmaceuticals. We have initially selected ovine gamma-interferon (gamma-IFN) as a target cytokine gene. By the use of the polymerase chain reaction (PCR), using primers based on the bovine gamma-interferon sequence, we have amplified the ovine gamma-interferon gene from crude messenger RNA extracted from lymphocytes. After cloning and DNA sequencing the gene, we found that ovine gamma-IFN is 93% identical to bovine gamma-IFN in amino acid sequence. This result indicates that the PCR method will be a rapid and efficient means for cloning other ovine cytokine genes.  相似文献   

Career aspirations of emerging veterinarians     

TJ HEATH  JS WESTERN 《Australian veterinary journal》1991,68(7):246-247

  相似文献   

Pathogenicity of Acholeplasma oculi for the sheep udder     

OP SADHANA  PP GUPTA  HS BANGA  JS RANA 《Australian veterinary journal》1993,70(6):227-228

  相似文献   

Development and trial of a bovine herpesvirus 1 - thymidine kinase deletion virus as a vaccine     

GA SMITH  PL YOUNG†  BJ RODWELL†  MA KELLY†  GJ STORIE†  CA FARRAH†  JS MATTICK† 《Australian veterinary journal》1994,71(3):65-70

SUMMARY An Australian bovine herpesvirus 1 (BHV1) isolate with a defined (427 base pair) deletion in the protein coding region of the thymidine kinase gene was obtained by standard marker rescue procedures. After selection in the presence of the nucleotide analogue 5iodo-deoxy-uridine the virus was analysed by hybridisation with three differential oligonucleotide probes, restriction endonuclease profile studies and DNA sequence analysis. The virus elicited an immune response in recipient animals after either intramuscular or intravenous administration and produced no significant deleterious side-effects when administered at a dose sufficient to stimulate the host immune response. The safety and immunogenicity of the recombinant BHV1 virus 39B1 were similar to those reported for other registered BHV1 vaccines and the virus would appear to be suitable for the production of a vaccine seed lot and more exhaustive field trials as a prelude to commercial vaccine production and registration.  相似文献   

Using 20-gauge percutaneous peripheral catheters to reliably collect serial 4-mL blood samples from conscious dogs     

KF Elliott  LM Fleeman  JS Rand 《Australian veterinary journal》2010,88(6):215-221

Objective To determine the reliability of collecting serial 4-mL blood samples over 13 h using a 20-gauge, cephalic catheter in conscious dogs. Design Prospective, observational study. Animals Twelve (6 males, 6 females) healthy, neutered, lean dogs. Procedure Percutaneous placement of a 20-gauge, 1.1 × 30 mm intravenous over-the-needle catheter into the cephalic vein was performed in each dog on three occasions. Catheter patency was maintained by intermittent flushing with 0.9% saline and 1 IU/mL heparinised saline solutions. Blood samples (4 mL) were obtained at 10 time-points over 13 h, with close monitoring of the dogs and the indwelling catheters. Blood volume, resistance on aspirating blood sample (minimal or marked resistance) and the methods used to improve blood flow were recorded. Results A high proportion (93%) of blood sample collections achieved the required 4-mL volume, and the remaining samples were greater than 1.5 mL. Of the 358 collections via the cephalic catheter, 311 (87%) were obtained with ‘minimal resistance’. The remaining collections had ‘marked resistance’ (n=47) and were associated with a loose catheter in 11% (5/47) and of unknown cause in 89% (42/47). After ‘marked resistance’ had been encountered and the catheter was flushed with saline and heparin solutions, subsequent sampling with ‘minimal resistance’ was frequently possible from the same catheter. Conclusion Use of a percutaneous, 20-gauge intravenous cephalic catheter allowed reliable serial collection of 4-mL blood samples over 13 h in conscious dogs.  相似文献   

Is Mycoplasma bovis a missing component of the bovine respiratory disease complex in Australia?     

PF Horwood  ML Schibrowski  EV Fowler  JS Gibson  TS Barnes  TJ Mahony 《Australian veterinary journal》2014,92(6):185-191

  相似文献   

Molecular epidemiology of clinical isolates of Pseudomonas aeruginosa isolated from horses in Ireland     

Tazumi A  Maeda Y  Buckley T  Millar B  Goldsmith C  Dooley J  Elborn J  Matsuda M  Moore J 《Irish veterinary journal》2009,62(7):456-459

Clinical isolates (n = 63) of Pseudomonas aeruginosa obtained from various sites in 63 horses were compared using ERIC2 RAPD PCR to determine their genetic relatedness. Resulting banding patterns (n = 24 genotypes) showed a high degree of genetic heterogeneity amongst all isolates examined, indicating a relative non-clonal relationship between isolates from these patients, employing this genotyping technique. This study characterised 63 clinical isolates into 24 distinct genotypes, with the largest cluster (genotype E) accounting for 10/63 (15.9%) of the isolates. ERIC2 RAPD PCR proved to be a highly discriminatory molecular typing tool of P. aeruginosa in isolates recovered from horses. With the adoption of several controls to aid reproducibility, this technique may be useful as an alternative to PFGE, particularly in epidemiological investigations of outbreaks where speed may be a significant parameter. This is the first report of clonal heterogeneity amongst P. aeruginosa from horses and demonstrated that ERIC RAPD PCR is a rapid method for the examination of this species in horses, which may be useful in outbreak analysis.  相似文献   

Orbital neoplasia in a dog     

AR DEYKIN  JS SMITH 《Australian veterinary journal》1997,75(9):638-640

  相似文献   

Efficacy of ivermectin controlled-release capsules for the control and prevention of nasal bot infestations in sheep   总被引：1，自引：0，他引：1  

D. RUGG  RP GOGOLEWSKI  RA BARRICK  JS EAGLESON 《Australian veterinary journal》1997,75(1):36-38

Objective To investigate the therapeutic and prophylactic efficacy of an ivermectin controlled-release capsule against nasal bots (Oestrus ovis) in sheep.
Design Trial 1 – A pen study with controls. Trial 2 – A field study with controls.
Animals Trial 1 – Forty Merino wethers with natural infestations of nasal bot were used. Trial 2 – One hundred nasal bot-free wethers were used.
Procedure Trial 1 – Ten randomly selected animals were slaughtered and the heads split and examined to confirm bot infestation. Fifteen animals were allocated to untreated controls and 15 to treatment with a controlled-release capsule delivering ivermectin at ≥ 20 μg/kg/day for 100 days. Twenty-nine days after treatment the sheep were killed and examined for nasal bots. Trial 2 – Nasal bot-free sheep were allocated to two groups of 45 animals. One group was untreated the other sheep were treated with capsules as above. The sheep were grazed as a single group exposed to natural challenge from O ovis . Ninety days after treatment the animals were slaughtered and examined for nasal bot infestation.
Results Trial 1 – Live O ovis larvae were recovered from 60% of control sheep. No live larvae were collected from treated sheep. Trial 2 – Forty-one percent of untreated sheep harbored nasal bot infestations. No live larvae were collected from any treated animal.
Conclusion Treatment with a single ivermectin controlled-release capsule was 100% effective against existing infestations of O ovis and as a prophylactic treatment for this parasite.  相似文献   

Intercomparison of snow Sampling and Analysis Within the Alpine-Wide Snowpack Investigation (SNOSP)     

Schwikowski  M.  Novo  A.  Baltensperger  U.  Delmas  R.  Gäggeler  H. W.  Kasper  A.  Kuhn  M.  Maupetit  F.  Nickus  U.  Preunkert  S.  Puxbaum  H.  Rossi  G. C.  Schöner  W.  Wagenbach  D. 《Water, air, and soil pollution》1997,93(1-4):67-91

In order to investigate the seasonal and geographical distribution of snow concentrations anddeposition fluxes of environmentally relevant ionic species in the Alps, the international programSNOSP was initiated. In the framework of this program, intercomparisons of snow samplingtechniques and analytical methods to determine the ionic species C1^-, NO ₃ ^- ,SO ₄ ^2- , K⁺, Na⁺, NH4 ⁺, Mg²⁺, and Ca²⁺, as well as the pH and the specificconductivity were performed. The concentrations of these species in the snow samples collectedin the SNOSP program varied by orders of magnitude with, e.g., concentrations of NO ₃ ^- , SO ₄ ^2- , and NH4 ⁺ ranging from 0.2-60, 0.2-90, and 0.1-60 µeq L^-1,respectively. The intercomparisons revealed a reasonable agreement of the determinations of thespecies Cl^-, NO ₃ ^- , SO ₄ ^2- , Na⁺, and NH4 ⁺ in snow. Results were less satisfactory for K⁺, Mg²⁺, Ca²⁺,and H⁺, mainly due to the very low concentrations. In conclusion, recommendations areformulated for the reliable derivation of chemical inventories from snow packs.  相似文献