Gonadotrophin–releasing hormone administered in continuous low dose can induce ovulation and normal corpora lutea in acyclic post–partum ewes and seasonally anoestrous ewes     

PJ WRIGHT  AH WILLIAMS  IJ CLARKE 《Australian veterinary journal》1994,71(4):123-125

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Chronic wasting disease in a Wisconsin white-tailed deer farm     

Delwyn P Keane  Daniel J Barr  Philip N Bochsler  S Mark Hall  Thomas Gidlewski  Katherine I O'Rourke  Terry R Spraker  Michael D Samuel 《Journal of veterinary diagnostic investigation》2008,20(5):698-703

In September 2002, chronic wasting disease (CWD), a prion disorder of captive and wild cervids, was diagnosed in a white-tailed deer (Odocoileus virginianus) from a captive farm in Wisconsin. The facility was subsequently quarantined, and in January 2006 the remaining 76 deer were depopulated. Sixty animals (79%) were found to be positive by immunohistochemical staining for the abnormal prion protein (PrP(CWD)) in at least one tissue; the prevalence of positive staining was high even in young deer. Although none of the deer displayed clinical signs suggestive of CWD at depopulation, 49 deer had considerable accumulation of the abnormal prion in the medulla at the level of the obex. Extraneural accumulation of the abnormal protein was observed in 59 deer, with accumulation in the retropharyngeal lymph node in 58 of 59 (98%), in the tonsil in 56 of 59 (95%), and in the rectal mucosal lymphoid tissue in 48 of 58 (83%). The retina was positive in 4 deer, all with marked accumulation of prion in the obex. One deer was considered positive for PrP(CWD) in the brain but not in the extraneural tissue, a novel observation in white-tailed deer. The infection rate in captive deer was 20-fold higher than in wild deer. Although weakly related to infection rates in extraneural tissues, prion genotype was strongly linked to progression of prion accumulation in the obex. Antemortem testing by biopsy of recto-anal mucosal-associated lymphoid tissue (or other peripheral lymphoid tissue) may be a useful adjunct to tonsil biopsy for surveillance in captive herds at risk for CWD infection.  相似文献   

Avian mycobacteriosis in captive brolgas (Antigone rubicunda)     

PJ Hodge  JR Sandy  AH Noormohammadi 《Australian veterinary journal》2019,97(3):81-86

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Agronomic factors affecting the yield and quality of forage maize in Ireland: effect of plastic film system and seeding rate   总被引：1，自引：0，他引：1  

G. P. Keane  J. Kelly  S. Lordan  K. Kelly 《Grass and Forage Science》2003,58(4):362-371

The objectives were to compare the effects of two plastic film systems [perforated (PP) and complete‐cover (CC) systems] on the yield and quality of forage maize, and to study the effects of seeding rate on maize grown with, and without, plastic film. Between 1995 and 1998 (Experiments 1 and 2), comparisons were made of maize without plastic film systems (NP), sown through clear plastic film (PP) and covered with clear plastic film (CC). In the CC system, the plastic was removed manually from the crop. Two dates of removal (CC1 and CC2) were compared in 1995, while four removal dates (CC1, CC2, CC3 and CC4) were examined in 1996–98. In 1996–98, three sowing dates (early, mid‐April; mid, early‐May; late, mid‐May) were also examined. Four seeding rates (78 200, 93 900, 107 000 and 126 000 seeds ha^?1) were examined in two experiments (Experiment 3 in 1996 and Experiment 4 in 1996–98). No plastic was used in Experiment 3, while three plastic treatments (NP, PP and CC) were examined in Experiment 4. In the CC treatment, the plastic film was removed at the six‐ to eight‐leaf stage. In Experiment 1, the PP treatment gave lower DM yields than the NP treatment due to frost damage which killed 0·30 of the plants in the PP treatment. Plants were undamaged by frost in both CC treatments, and the CC2 treatment (plastic removal at the six‐leaf stage) gave significant increases in DM and grain yields. In Experiment 2, averaged over years and sowing dates, DM yields were significantly increased by all plastic film treatments except CC4. All plastic film treatments gave significantly earlier tassel emergence than the NP treatment, and significantly increased grain yields and contents of DM and grain. The highest yields of DM and grain were obtained from the PP system. Within the CC treatments, leaving the plastic film intact until the ten‐leaf stage (CC4) gave lower yields of DM and grain than removing the plastic film at an earlier stage. Averaged over years, the largest DM yields were obtained from the CC system with early‐sown material, while the yield differences between treatments with late‐sown crops under this system were not significant. Highest DM and grain yields were obtained with early sowing, while late sowing gave lower contents of DM and grain, and later tassel emergence. In Experiments 3 and 4, DM and grain yields increased as seeding rate increased up to 126 000 seeds ha^?1 with NP and CC plastic treatments. With the PP treatment, DM yield reached a plateau at 107 000 seeds ha^?1. Seeding rate did not significantly affect DM content in either experiments but grain content declined in both experiments as seeding rate increased.  相似文献   

A detached-leaf method to evaluate late blight resistance in potato and tomato     

R. W. Goth  J. Keane 《American Journal of Potato Research》1997,74(5):347-352

We developed a detached leaf method for evaluating potato (Solanum tuberosum L.) and tomato (Lycopersicon esculentum Mill.) germplasm for reactions toPhytophthora infestans, the causal organism of late blight. Primary leaves from the third to the sixth node of potato plants, and the fourth to sixth node of tomato plants were excised at the stem. Their petioles were inserted into 14 mm × 100 mm floral aqua tubes containing 9 ml of sterilized distilled water. The leaves in the aqua tubes were placed with abaxial sides down on galvanized metal hardware cloth (12.5 × 12.5 mm mesh). A 12.5 mm sensi-disc containing 50μl of 2 × 10⁴ zoospores was placed in the centers of the terminal leaflet and the second leaflet pair of the potato leaf. A single disc was placed on the center of the tomato leaf. The supporting hardware cloth was placed 2.5 cm above distilled water (2.5 cm deep) in 31 cm × 17 cm × 8 cm clear plastic boxes with tight fitting lids. Leaves on intact tomato and potato plants were inoculated in a similar manner and placed in a mist chamber. Lesion growth was determined 4, 5, and 6 days following inoculation. There were no significant differences in reactions to isolates ofP. infestans on detached and intact leaves of potato cultivars Green Mountain and Kennebec and the tomato cultivar Bonnie Best.  相似文献   

Efficacy of a buccal meloxicam formulation for pain relief in Merino lambs undergoing knife castration and tail docking in a randomised field trial     

AH Small  S Belson  M Holm  IG Colditz 《Australian veterinary journal》2014,92(10):381-388

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Use of a multifenestrated indwelling lavage system for treatment of septic digital teno-synovitis in cattle     

DE ANDERSON  D. ALLEN  G. ST-JEAN  AH PARKS 《Australian veterinary journal》1997,75(11):796-799

Septic tenosynovitis was diagnosed in seven cattle on the basis of history, physical examination, radiographs, cytological examination of tendon sheath fluids, and microbial culture. A commercially available indwelling multifenestrated silicone rubber drain was used to perform frequent lavage of the flexor tendon sheaths. The sepsis resolved in all cattle. Five of six cattle for which long-term (> 1 year) follow-up information was available were clinically sound on the affected limb and had remained productive members of the herd.  相似文献   

Phytophthora cinnamomi and the diseases it causes: by George A. Zentmeyer. Monograph No. 10, The American Phytopathological Society, 1980. 69 pp. U.S. $8.00     

P.J. Keane 《Soil biology & biochemistry》1981,13(6):559

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CHARACTERIZATION OF PHYSIOLOGIC 18F‐FDG UPTAKE WITH PET‐CT IN DOGS     

MIN‐SU LEE  AH‐RA LEE  MI‐AE JUNG  IN‐HYE LEE  JI‐HYE CHOI  HYUN‐WOO CHUNG  SOON‐WUK JEONG  SANG‐SOEP NAHM  KI‐DONG EOM 《Veterinary radiology & ultrasound》2010,51(6):670-673

We evaluated the whole body distribution of 2‐deoxy‐2‐[18F]fluoro‐d ‐glucose (¹⁸F‐FDG) in seven beagle dogs using positron emission tomography/computed tomography. The mean and maximum standard uptake values (SUV) for various tissues were computed. The SUV of the aortic blood pool was 0.65±0.19. Moderate uptake was present in brain (3.40±1.01). Mild uptake was present in orbital muscles, soft palate, laryngeal and pharyngeal region, mandibular salivary gland, myocardium, liver, pancreas, kidney, and intestine. ¹⁸F‐FDG uptake would be normally higher in these tissues because of normal physiologic activity. Mean and maximum SUV values of the eye, skeletal muscle, bone tissue, spleen, adrenal gland, stomach, tongue, gall bladder, and lung were similar to or lower than that of the aortic blood pool. These data provide a normal baseline for comparing pathologic ¹⁸F‐FDG uptake.  相似文献   

Development of a recombinant antibody ELISA test for the detection of Polymyxa betae and its use in resistance screening   总被引：6，自引：1，他引：5  

C. S. Kingsnorth  M. J. C. Asher †  G. J. P. Keane  D. M. Chwarszczynska  M. C. Luterbacher  E. S . Mutasa-Göttgens 《Plant pathology》2003,52(6):673-680

An ELISA test was developed for the quantitative detection of the obligate parasite Polymyxa betae , the vector of Beet necrotic yellow vein virus (BNYVV), in infected sugarbeet roots. The test used monoclonal and polyclonal antibodies raised to a recombinantly expressed glutathione-S-transferase (GST) from P. betae . A close correlation was found between the number of P. betae zoospores in serially diluted suspensions and absorbance values in the ELISA test. Time-course studies of plants grown in naturally infested soils in controlled environment tests demonstrated the value of the ELISA test in screening for P. betae resistance. In preliminary tests, P. betae -resistant accessions of the wild sea beet ( Beta vulgaris ssp. maritima ), which might be used to restrict the transmission of BNYVV, were identified.  相似文献