Development and evaluation of a rapid absorbed enzyme immunoassay test for the diagnosis of Johne''s disease in cattle   总被引：8，自引：0，他引：8  

JC COX  DP DRANE  SL JONES  S. RIDGE†  AR MILNER† 《Australian veterinary journal》1991,68(5):157-160

An absorbed enzyme immunoassay (EIA) test for Johne's disease in cattle was developed in which absorption of cross-reacting antibodies occurred as a rapid reaction in solution rather than overnight with whole organisms and a subsequent centrifugation step. Total test time was reduced to less than 2 h with a minimum of manipulations. The test was evaluated in cattle herds from Johne's disease-endemic and Johne's disease-free regions of Australia. Specificity was 99.8%. Calculations of sensitivity were affected by the history of the herd under test. However, the EIA detected in excess of 80% of animals before onset of clinical disease and 65% of faecal shedders were EIA positive on, or before, first detection of Mycobacterium paratuberculosis in their faeces. The test should aid epidemiological studies and be a useful tool in the management and control of Johne's disease.  相似文献   

Isolation of Haemophilus Taxon "minor group" from pigs     

PJ BLACKALL  AR MERCY  N BULLER  J DICKSON  R FOGARTY  D JAMESON 《Australian veterinary journal》1991,68(3):119-120

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The retrospective diagnosis of a second outbreak of equine morbillivirus infection   总被引：5，自引：0，他引：5  

PT HOOPER  AR GOULD  GM RUSSELL  JA KATTENBELT  G. MITCHELL 《Australian veterinary journal》1996,74(3):244-245

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Suspected Cryptostegia grandiflora (rubber vine) poisoning in horses     

DR COOK  GW CAMPBELL  AR MELDRUM 《Australian veterinary journal》1990,67(9):344-344

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Disease of geese caused by a new herpesvirus   总被引：3，自引：0，他引：3  

PJ KETTERER  BJ RODWELL  HA WESTBURY†  PT HOOPER†  AR MACKENZIE‡  JG DINGLE§  HC PRIOR 《Australian veterinary journal》1990,67(12):446-448

A goose flock farmed outdoors in south-eastern Queensland suffered an outbreak of peracute disease with high death rate (97%). Small button ulcers and large plaques overlying lymphocyte aggregates were present on the mucosa of the small intestine of affected birds. Small white foci of necrosis and focal haemorrhages were seen in the livers. Numerous intranuclear inclusion bodies were observed microscopically in hepatocytes and a herpesvirus which grew rapidly in chicken kidney cells was isolated from tissues. Duck virus enteritis (DVE) was suspected but DVE antiserums failed to neutralise the virus. Further serological studies with a limited range of known avian herpesviruses have failed to identify the virus. Experimental transmission resulted in high mortality in geese (100%), lower mortality in ducklings and nil mortality in chickens. Surveillance studies showed no evidence of infection in domestic and wild birds beyond the original farm and the infection appears not to have been established in the area. Wild ducks, which were frequent visitors to the farm dam, were considered the most likely source of the infection.  相似文献   

Gross Morphometry of the Bovine Placentome during Gestation     

RA Laven  & AR Peters 《Reproduction in domestic animals》2001,36(6):289-296

Forty-seven pregnant uteri were collected from an abattoir at eight chronological stages of gestation. Assessments were made of placentome number, weight, length shape and ratio of foetal to maternal tissue by weight. The study showed that there was a great variation in number, size, weight and shape of placentomes. There was no significant change in the total number of placentomes during gestation. Both mean weight and length increased significantly during gestation, but there was a significant change in the relationship between the two as gestation progressed. There was no correlation between the number of placentomes and the mean weight or length of the placentomes. The development of the placenta in the non-pregnant horn was significantly different from that of the pregnant horn, with fewer, smaller, lighter placentomes. However, there was no evidence that placentome development in the non-pregnant horn was affected by placentome development in the pregnant horn. The proportion of the placentome that was maternal tissue increased during gestation, but there was no significant effect of placentome number, or individual placentome weight or length on this proportion. Two placentome shapes were identified, but no effect on foeto-maternal proportion or relationship between shape and total placentome number or weight was identified.  相似文献   

Molecular pathotyping of two avian influenza viruses isolated during the Victoria 1976 outbreak   总被引：3，自引：0，他引：3  

JB BASHIRUDDIN  AR GOULD  HA WESTBURY 《Australian veterinary journal》1992,69(6):140-142

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Survey of internal parasites in Western Australian pig herds 2. Relationship to anthelmintic usage and parasite control practices     

AR. MERCY  G. de.  CHANEET † Y. EMMS 《Australian veterinary journal》1989,66(1):6-9

The housing, management and internal parasite control practices used in piggeries in Western Australia was surveyed by interviewing the owner/manager of 100 randomly selected piggeries. At the time of the interview 20 faecal samples were collected from pigs and examined for evidence of internal parasites. Most herds (80%) in which anthelmintics were used, had evidence of nematode infection. Some pig producers were using an anthelmintic that was ineffective against nematode species present while some herds had nematodes that should have been controlled by the anthelmintic being used. Prevalence of nematode infection was higher in pigs run in paddocks than in pigs housed intensively. Washing sows prior to farrowing was associated with a lower prevalence of nematode infection. The findings suggested that pig producers should ensure that the anthelmintic used is effective against the nematodes present in their pigs. It was concluded that administering anthelmintics as a single dose in the feed may not be the most effective method of deworming pigs.  相似文献   

Phylogenetic characterisation of bluetongue viruses from naturally-infected insects, cattle and sheep in Australia   总被引：1，自引：0，他引：1  

KA McCOLL  AR GOULD  LI PRITCHARD  L MEL VILLE†  G. BELLIS† 《Australian veterinary journal》1994,71(4):102-105

SUMMARY The polymerase chain reaction was used to detect the presence of blue-tongue virus (BTV) in a number of clinical and insect samples collected in the Northern Territory of Australia. Sequence analyses of the amplified BTV genes differentiated endemic Australian and exotic viruses. Two potential exotic BTV were detected as a result of PCR analyses of blood from sentinel animals and of the insect vector, Culicoides wadai. The detection of BTV in C wadai was the first direct demonstration of the presence of BTV in this potential vector. This new technology can significantly reduce the time taken for a diagnosis from a clinical sample and increase the amount of useful information obtained on a BTV isolate by using rapid sequencing techniques. Sequence data were used to differentiate between BTV20 isolated in 1975 and two isolates of the same serotype, isolated in 1992, and indicated that the latter were probably a recent incursion into Australia from Indonesia due to their greater VP3 sequence homology to the BTV9 (Java) than to Australian BTV isolates.  相似文献   

A Bayesian meta-analysis of the effects of administering an intra-vaginal (CIDR) device in combination with other hormones on the reproductive performance of cycling,anoestrous and inseminated cows     

AR Rabiee  IJ Lean  MA Stevenson 《New Zealand veterinary journal》2013,61(6):384-393

AIMS: To evaluate the effectiveness of treatment programmes that included controlled internal drug-releasing (CIDR) devices containing progesterone (P4) in improving synchrony of oe- strus, and conception and pregnancy rates in cycling, anoestrous and inseminated dairy cows, using meta-analysis. To describe the difference in response between cycling and anoestrous cows to CIDR-based synchrony programmes.

METHODS: Scientific papers written in the English language between 1989 and 2002 that investigated the effects of treat- ment programmes including CIDR devices on reproductive per- formance in dairy heifers or lactating dairy cows were identified using a computerised literature search. The criteria for inclusion incorporated evidence that treatment allocation was completely randomised; the population studied was lactating dairy cows; and that data were available on submission, conception and pregnancy rates and their associated measures of variability. Re- productive outcomes from 25 synchrony trials (total n= 11,058 cows) were analysed. Summary measures of the effect of treat- ment on reproductive outcome were assessed using fixed- and random-effects Bayesian meta-analysis models.

RESULTS: Treatment programmes including a CIDR device increased the risk of submission in cycling cows (predicted Bayesian RR=2.86, 95% credible interval 1.46–5.67). Compared with controls, synchrony programmes including CIDR devices in cycling dairy cows had no effect on the risk of conception to first service post-treatment (predicted Bayesian RR=1.00, 95% credible interval=0.80-1.24). Compared with controls, synchrony programmes including CIDR devices had no effect on the risk of pregnancy throughout the mating period (pre- dicted Bayesian RR=1.02, 95% credible interval=0.89-1.17). In anoestrous cows, CIDR treatment had no effect on the risk of conception to first service post-treatment and no effect on the risk of pregnancy throughout the mating period, compared with anoestrous, untreated controls (predicted Bayesian RR=0.91 and 0.97, respectively; 95% credible interval=0.68–1.26 and 0.59-1.60, respectively).

CONCLUSION: The results of this meta-analysis showed that synchrony programmes using CIDR devices combined with other hormones reliably enhanced submission rates in lactat- ing dairy cows. The relatively small number of trials with data suitable for analysis and the heterogeneity of results at the indi- vidual trial level limited our ability to confirm either a beneficial or deleterious effect of treatment on conception or pregnancy rates. Further randomised, controlled trials to evaluate the ef- fectiveness of this form of reproductive therapy in commercial dairy farms are needed.  相似文献