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11.
Joo Youn Oh † ‡ Mee Kum Kim † ‡ Jung Hwa Ko † ‡ Hyun Ju Lee † ‡ Jin Hak Lee † Won Ryang Wee † ‡ 《Veterinary ophthalmology》2009,12(S1):35-40
Objective Recent reports have demonstrated that mesenchymal stem cells (MSCs) can modulate/suppress immunologic responses through interactions with different immune cells. We performed this study in order to investigate the immunomodulatory effects of MSCs in corneal xenotransplantation.
Animals studied Pig and rat.
Procedures We orthotopically transplanted pig corneas into rats and topically applied allogeneic rat MSCs to the corneas for 2 h immediately after transplantation. Graft survival was clinically assessed using slit-lamp biomicroscopy and the median survival time (MST) was calculated. The rejected grafts were histologically examined using antibodies against CD4, CD8, CD161, and CD68. The expression of IL-2, IL-6, IL-10, and IFN-γ was also evaluated in the rejected grafts using an enzyme-linked immunosorbent assay.
Results The survival of corneal xenografts was not significantly prolonged by MSC application (MST 10.5 days) compared with the controls (MST 9.67 days) ( P = 0.4189). Histologically, the rejected grafts in both groups were massively infiltrated with neutrophils and macrophages. Some CD8+ T cells and rare NK cells were found in the rejected grafts. The levels of IL-6 and IL-10 were significantly increased in the rejected grafts from MSC-treated rats compared with the grafts from MSC-untreated rats. However, the levels of IL-2 and IFN-γ were not different between the two groups.
Conclusions Topical application of allogeneic rat MSCs was ineffective in prolonging corneal xenograft survival in a pig-to-rat model. 相似文献
Animals studied Pig and rat.
Procedures We orthotopically transplanted pig corneas into rats and topically applied allogeneic rat MSCs to the corneas for 2 h immediately after transplantation. Graft survival was clinically assessed using slit-lamp biomicroscopy and the median survival time (MST) was calculated. The rejected grafts were histologically examined using antibodies against CD4, CD8, CD161, and CD68. The expression of IL-2, IL-6, IL-10, and IFN-γ was also evaluated in the rejected grafts using an enzyme-linked immunosorbent assay.
Results The survival of corneal xenografts was not significantly prolonged by MSC application (MST 10.5 days) compared with the controls (MST 9.67 days) ( P = 0.4189). Histologically, the rejected grafts in both groups were massively infiltrated with neutrophils and macrophages. Some CD8
Conclusions Topical application of allogeneic rat MSCs was ineffective in prolonging corneal xenograft survival in a pig-to-rat model. 相似文献
12.
Isolation of a soil bacterium capable of biodegradation and detoxification of endosulfan and endosulfan sulfate 总被引：2，自引：0，他引：2
Lee JB Sohn HY Shin KS Jo MS Kim JE Lee SW Shin JW Kum EJ Kwon GS 《Journal of agricultural and food chemistry》2006,54(23):8824-8828
Endosulfan, an endocrine disrupting chemical, is a widely used cyclodiene organochlorine pesticide worldwide, and it blocks neuronal GABA(A)-gated chloride channels in mammals and aquatic organisms. Endosulfan and its metabolites, such as endosulfan sulfate, are persistent in environments and are considered as toxic chemicals. For bioremediation of endosulfan, in this study, an attempt was made to isolate an endosulfan and endosulfan sulfate degrading bacterium from endosulfan-polluted agricultural soil. Through repetitive enrichment and successive subculture using endosulfan or endosulfan sulfate as the sole carbon source, a bacterium KS-2P was isolated. The KS-2P was identified as Pseudomonas sp. on the basis of the results of a 16S rDNA sequencing analysis and MIDI test. The degradation ratios for endosulfan or endosulfan sulfate in minimal medium containing endosulfan (23.5 microg mL(-1)) or endosulfan sulfate (21 microg mL(-1)) were 52% and 71%, respectively. Our results suggest that Pseudomonas sp. KS-2P has potential as a biocatalyst for endosulfan bioremediation. 相似文献
13.
Joo Youn Oh Mee Kum Kim Hyun Ju Lee Jung Hwa Ko Won Ryang Wee Jin Hak Lee 《Veterinary ophthalmology》2009,12(Z1):50-56
Objective Although variations exist between species with respect to outcomes after cryopreservation, little is known about the differences in the susceptibility of the corneal stroma to cryoinjury. We performed this study to investigate freeze–thaw‐induced damage in keratocytes and collagen in rabbit, pig, and human corneas. Animals studied Rabbit, pig, and human. Procedures We prepared 250‐μm‐thick anterior stroma from rabbit, pig, and human corneas after scraping off the epithelium and endothelium. Each 250‐μm‐thick corneal stroma without epithelium was placed in a 50‐mL tube, frozen with liquid N2 for 15 min and taken out to thaw rapidly at 37 °C. This procedure of rapid freezing and thawing was repeated three times. Differences between the species with respect to cells and collagen structures were examined using hematoxylin–eosin (H&E) staining, terminal deoxynucleotidyl transferase‐mediated nick end labeling (TUNEL) assay, and transmission electron microscopy (TEM). We orthotopically transplanted the pig and rabbit corneal transplants after the triple freeze–thaw cycle into rabbit eyes and evaluated graft survival. Results On gross examination, rabbit corneas became opaque after the triple freeze–thaw procedure, while pig and human corneas remained transparent. Histologically, keratocytes were apoptotic on TUNEL assay and TEM in rabbit, pig, and human corneas. Collagen fibrils were fragmented and the arrangement of collagen fibrils was severely disturbed in rabbit corneas on H&E staining and TEM; collagen was well preserved in pig and human corneas. Rabbit corneal stroma underwent autolysis after transplantation, whereas the pig corneal stroma remained clear for 1 month. Conclusions Our study showed that rabbit corneal stroma was more susceptible to freeze–thaw injury than pig and human corneas. 相似文献
14.
U. Eren S. Kum M. Sand&#;kç &#; V. Eren F. Ilhan 《Anatomia, histologia, embryologia》2009,38(4):286-291
The aim of this study was to determine and examine the distribution of major frequency MHC II+ cells in the oviduct and vagina of cows during the oestrous and dioestrus phases. Right oviduct (ampulla, isthmus) and vaginal samples taken from a total of twenty seven multiparous cows were used. Tissue samples were processed to obtain both cryostat and paraffin sections. Sections were stained immunocytochemically using StreptABC method using a specific monoclonal antibody to MHC II+ cell population. Intra-epithelial and subepithelial areas along with lamina propria, muscularis mucosae and serosa of both ampulla and isthmus and intra-epithelial/subepithelial areas and mucosae of vagina were examined for the presence of MHC II+ cells. The density of immune positive cells was determined using a subjective scoring system. MHC II+ cells were demonstrated in all areas examined in both oestrus and dioestrus. In oestrus, the density of MHC II+ cells decreased in subepithelial areas (in between the epithelial cells and the basal membrane) of isthmus, whereas the density of immune positive cells was increased in muscularis mucosae of isthmus ( P < 0.05), lamina propria and muscularis mucosae of ampulla ( P < 0.05) as well as in the mucosae of vagina ( P ≤ 0.005). This study indicates that the density of MHC II+ cells observed in the oviduct and vagina increases in the majority of areas examined due to the effect of oestrogen. 相似文献
15.
Joon-Young Jun Min-Jeong Jung Jun-Seok Kum Gwang-Woo Kim Jae-Hyung Jung Jae-Man Sim 《Journal Of Aquatic Food Product Technology》2020,29(6):520-530
ABSTRACT in this study, physiochemical changes occurring during the preparation of smoked mold ripened products from mackerel (Scomber japonicus) meat were investigated. This process included filleting, steaming, smoking, vacuum-drying, and mold ripening. After steaming, the moisture content and water activity in the mackerel meat were reduced to 24% and 0.82, respectively. The protein and lipid contents were unchanged (P < .05), but saturated and monoenic fatty acid levels decreased. During the 30 days of mold ripening with Aspergillus repens KCCM 60286, the contents of ATP-related compounds and protein were negligibly degraded, while free fatty acid content increased without increasing thiobarbituric acid value. 相似文献
16.
Lee Ji Min Park Youn Shik Kum Donghyuk Jung Younghun Kim Bomchul Hwang Soon Jin Kim Hyun Bae Kim Chulgoo Lim Kyoung Jae 《Paddy and Water Environment》2014,12(1):169-183
Aquatic ecosystems are threatened by increasing variability in the hydrologic responses. In particular, the health of river ecosystems in steeply sloping watersheds is aggravated due to soil erosion and stream depletion during dry periods. This study suggested and assessed a method to improve the adaptation ability of a river system in a steep watershed. For this, this study calibrated soil and water assessment tool (SWAT) for runoff and sediment, and quantified the changes in hydrologic responses such as groundwater recharge rate soil erosion and baseflow according to two scenarios for adjustment of the watershed slope (steep to mild). Here, one scenario was set by three measured slopes, and the other was set by fixing the entire watershed slopes with 5 %. Moreover, SWAT and web-based hydrograph analysis tool (WHAT) models were applied to estimate groundwater recharge, soil erosion, and baseflow in the Haean-myeon watershed in South Korea. The results show that the reduction of watershed slope increased groundwater recharge and baseflow, and decreased sediment. Specifically, groundwater recharge rate was increased from 257.10 to 364.60 mm, baseflow was increased from 0.86 to 1.19 m3/s, and sediment was decreased from 194.6 to 58.1 kg/km2. Based on these results, the suggested method will positively contribute to aquatic ecosystems and farming environments in a steeply sloping watershed due to improvements in the quantity and quality of river water. 相似文献