Fireblight situation in Bulgaria and measures undertaken by the NPPO1     

E. Dimitrova  L. Andreev 《EPPO Bulletin》2004,34(3):343-345

Fireblight disease, caused by Erwinia amylovora was first detected in Bulgaria on quince in the region of Plovdiv in 1989. The disease was initially localized in that area but, during 1995/1997, due to favourable climate conditions, it became epidemic. Infected trees were grubbed out and destroyed. The main hosts are quince and pear (over 40% of affected trees), then apple, medlar and Cotoneaster. Containment measures undertaken by the Bulgarian NPPO are laid down in the Plant Protection Law, in Phytosanitary Regulation no. 1 for phytosanitary control, and in Phytosanitary Regulation no. 5 of 1996 (amended 1997) for containment of fireblight. Phytosanitary control is mainly focused on fruit‐tree nurseries and on the distribution of healthy plants for planting. In 2003, 41 protected zones and 29 protected sectors within infested areas have been established.  相似文献   

Flow cytometric analysis of intracellular complexity and CD45 expression for use in rapid differentiation of leukocytes in bovine blood samples.     

L S Pelan-Mattocks  B A Pesch  M E Kehrli 《American journal of veterinary research》2001,62(11):1740-1744

OBJECTIVE: To develop an efficient and reliable method that accurately differentiates bovine lymphocytes from monocytes in leukograms. SAMPLE POPULATION: Blood samples from 30 healthy cows and 1 calf with bovine leukocyte adhesion deficiency. PROCEDURE: Flow cytometric analysis of intracellular complexity and CD45 expression on bovine leukocytes was compared with results for conventional light microscopy methods. Verification of leukocyte subpopulations determined by intracellular complexity and CD45 expression was conducted, using 2-color phenotypic analysis with selected monoclonal antibodies. RESULTS: The CD45 and side-scatter properties of bovine leukocytes clearly differentiated cell types, including neutrophils, eosinophils, monocytes, and lymphocytes. CONCLUSIONS AND CLINICAL RELEVANCE: This is a rapid assay that is simple to use. More importantly, it is more accurate than the conventional method that involves the use of blood slides and light microscopy, because of the ability of the assay to readily distinguish bovine monocytes and lymphocytes. Rapid preparation of samples and short analysis times allow for efficient and reliable examination of a large number of samples, and the task of viewing slides by light microscopy is eliminated. The labor-savings benefit of this procedure is most apparent in research environments that require frequent processing of batches of blood samples.  相似文献   

角形区域的Teichmuler极值映照     

程金发  诸玉明  顾广泽 《湖南农业大学学报(自然科学版)》1996,23(5)

采用二次微分的方法，得到了角形区域Ω１的Ａｆｆｉｎｅ变换关于其边界值不是极值映照．并明确给出在边界同伦下唯一极值的Ｔｅｉｃｈｍｕｌｅｒ映照．  相似文献   

Genetic diversity of herbicide-resistant and -susceptible Avena fatua populations in North Dakota and Minnesota     

L W MENGISTU  C G MESSERSMITH  & M J CHRISTOFFERS 《Weed Research》2005,45(6):413-423

Genetic diversity within and among 20 herbicide-resistant (HR) and 16 herbicide-susceptible (HS) Avena fatua multi-field populations was determined using 82 polymorphic loci resulting from two intersimple sequence repeat (ISSR) primers and one long-primer random amplified polymorphic DNA (LP-RAPD) primer. Collections from the Red River Valley of North Dakota and Minnesota, sampled in 1964 and 2000, represented A. fatua populations before and after intensive exposure to herbicides. A 1995 collection from south-west North Dakota represented A. fatua exposed to low herbicide selection. Despite differences in years of herbicide exposure among collections, both HR and HS populations from every collection maintained nearly similar levels of ISSR and RAPD diversity. Genetic differentiation among populations (G_ST) varied from 11% to 13% among HR populations and from 9% to 16% among HS populations, indicating that 84–91% of total variation remained within HS or within HR populations. Minimal difference in gene diversity between HR and HS is consistent with multiple origins of resistance, where HR A. fatua most likely evolved from diverse founding individuals.  相似文献   

APPARATUS TO ASSIST IN PHOTOGRAPHING EXPERIMENTAL MATERIAL     

Addington LH 《Science (New York, N.Y.)》1938,88(2284):335-336

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GROWTH STIMULATION OF PEAS BY TETRACHLORO-PARA-BENZOQUINONE,A FUNGICIDAL SEED PROTECTANT     

McNew GL 《Science (New York, N.Y.)》1942,96(2483):118-119

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Poultry     

C. L. Wright 《Tropical animal health and production》1991,23(2):102-102

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Preliminary study on owner-reported behaviour changes associated with chronic pain in dogs.     

M L Wiseman  A M Nolan  J Reid  E M Scott 《The Veterinary record》2001,149(14):423-424

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Vaccination of the brushtail possum (Trichosurus vulpecula) against Mycobacterium bovis infection with bacille Calmette-Guérin: the response to multiple doses.     

L A L Corner  B M Buddle  D U Pfeiffer  R S Morris 《Veterinary microbiology》2002,84(4):327-336

In New Zealand, the brushtail possum (Trichosurus vulpecula) is the principal wildlife vector of bovine tuberculosis. Control of infected possum populations contributes to the control of tuberculosis in domestic livestock. Vaccination is potentially a complementary strategy to population control, but to be cost-effective, administration of the vaccine to possums would need to be from an appropriately designed automatic vaccinator. Possums themselves would activate the vaccinator so that it would deliver an aerosol spray of vaccine. There would be no direct way to prevent possums receiving multiple doses of vaccine. This study examined the effect on protective immunity of repeated vaccination. Captive possums were vaccinated with BCG strain pasteur 1173P2 either 12 times at weekly intervals, twice at 6-weekly intervals, or once. Vaccination was by a combination of intranasal aerosol and conjunctival instillation. Eight weeks after the last dose of vaccine, all possums were challenged intratracheally with Mycobacterium bovis strain 83/6235. Vaccination induced a significant immune response as measured by the lymphocyte proliferation assay (LPA). A significant level of protection, as measured by the response to challenge, developed in all the vaccinated possum groups, but protection was greatest in the group vaccinated 12 times. It was concluded that protection would be enhanced if vaccinations were repeated at short intervals (weekly), but no benefit or detriment resulted from revaccination after longer intervals (1-2 months).  相似文献   

Study on Site-specific Nutrient Management in Cotton Field     

YANG Li-ping  Jiang Cheng  JIN Ji-yun  ZHANG Feng-ming 《中国农业科学(英文版)》2001,(1):102-106

The study on the characteristics of spatial variability of soil nutrients and fertilizer recommendations in cotton field under certain conditions of agricultural management was conducted with GIS and systematic approach for soil nutrient constrains. The results showed that of the spatial variability of soil nutrient was greatly related to the management condition of previous crops. Grid sampling and variable rate application technology (VRAT) were the tools that would hopefully increase fertilizer efficiency. The fertilizers were applied where they were needed and at proper rate. Balance fertilization demonstration showed that fertilizer recommendations according to the available nutrient level in soil could decrease fertilizer cost with 657.4 yuan / ha and increase seed cotton yield by 19.8%. A net profit of the balanced fertilization was 5314.9 yuan / ha higher than that of local fertilization practice.  相似文献