Viral and bacterial agents associated with experimental transmission of infectious proventriculitis of broiler chickens.   总被引：1，自引：0，他引：1  

G R Huff  Q Zheng  L A Newberry  W E Huff  J M Balog  N C Rath  K S Kim  E M Martin  S C Goeke  J K Skeeles 《Avian diseases》2001,45(4):828-843

Proventriculitis of broilers can be reproduced by oral inoculation of day-old chicks with a proventricular homogenate from affected 3-wk-old broilers. The objective of the following studies was to isolate from this homogenate viral and bacterial isolates that could produce proventriculitis. A monoclonal antibody to infectious bursal disease virus (IBDV) was used to precipitate virus from the homogenate. A primary chicken digestive tract cell culture system was also used to isolate virus from a 0.2-microm filtrate of the homogenate, and a bacterium was also isolated from the homogenate. In trial 1, day-old birds were orally inoculated with either proventriculus homogenate or monoclonal antibody immunoprecipitated IBDV (MAB-IBDV). At 4, 7, 14, and 21 days postinfection (PI), 12 birds from each treatment group were subjected to necropsy. In trial 2, day-old birds were orally inoculated with either infectious proventriculus homogenate, suspect virus isolated in cell culture and propagated in embryo livers and spleens, or a bacterial isolate. Twelve birds from each treatment were subjected to necropsy at days 7, 14, 21, and 28 PI. In trial 3, treatments were maintained in negative pressure isolation chambers, and an additional treatment included virus plus bacterial isolate. Twenty-four birds from each treatment were subjected to necropsy at day 21 PI. In trial 1, infectious homogenate decreased body weight and relative gizzard weights at 4, 7, 14, and 21 days PI. Proventriculus relative weight was increased at days 7, 14, and 21 PI, and proventriculus lesion scores were increased at days 14 and 21 PI. Bursa/spleen weight ratios were decreased at day 14, and feed conversion was increased at days 4 and 21. The MAB-IBDV treatment decreased proventriculus and gizzard relative weights at day 4 PI, increased proventriculus lesion scores and bursa/spleen weight ratios at day 14, and decreased heterophil/lymphocyte ratios at day 21. In trial 2, all infected birds had significantly higher mean relative proventriculus weights at 21 days PI and had higher 4-wk mean proventriculus scores as compared with both control groups. In trial 3, birds treated with homogenate and birds treated with both suspect virus and the bacterial isolate had significantly higher proventriculus lesion scores; higher relative weights of proventriculus, gizzard, liver, and heart; lower body weights; and lower relative bursa weights compared with the saline control group. These studies suggest that infectious proventriculitis has a complex etiology involving both viral and bacterial infection.  相似文献   

猪细小病毒氢氧化铝疫苗研究   总被引：3，自引：0，他引：3  

韩孝成  董齐  杨奇伟  刘惠敏  王润芝  金岳  李宝启 《中国预防兽医学报》2001,23(5):381-385

选用从国内浙江省某猪场流产死胎中分离的猪细小病毒强毒（简称浙PV－Z株），通过仔猪肾原代细胞增殖后，经甲醛灭活，再配以20％的氨氧化铝胶佐剂制成猪细小病毒氢氧化铝疫苗。该疫苗免疫性好，免疫程序简单。小剂量肌注豚鼠和猪，均能激起明显的抗体应答反应，疫苗对豚鼠接种2次HI价可达到1：320以上（通常1：640－1：1280）。对成年猪接种疫苗0．2ml,0.5ml,1.0ml免疫2次，或用2．0ml免疫1次，HI价范围在1：20－1：320之间，疫苗对胎儿的保护率可达到98．7％。实验室和田间跟踪试验显示该疫苗具有保存期长、耐热、安全、免疫保护率高等特点。  相似文献   

Prevalence of ovine and bovine respiratory syncytial virus infections in cattle determined with a synthetic peptide-based immunoassay.     

S T Grubbs  S A Kania  L N Potgieter 《Journal of veterinary diagnostic investigation》2001,13(2):128-132

Subgroup-specific peptide-based enzyme-linked immunosorbent assays from the G-protein of the ovine and bovine respiratory syncytial virus (RSV), respectively, were used to determine the prevalence of the ovine and bovine subgroup strains of RSV infections in cattle. A total of 1,102 bovine serum samples were obtained from 6 diagnostic laboratories located in the northwestern and the southeastern USA and were tested for antibody to either the bovine or ovine subgroups of RSV. Antibody to viruses from each subgroup was present in samples from each region and all states tested. The Southeast had a higher prevalence of the bovine subgroup strains (69.5%). Then did the Northwest (40.9%). The prevalence of the ovine strain was similar for the two regions (16.7% in the southeast, 14.9% in the northwest). The overall prevalence was 56.6% for the bovine strain and 15.9% for the ovine strain. These results suggest members of the ovine subgroup of RSV circulate in the cattle population but with less frequency than those viruses of the bovine subgroup.  相似文献   

Genotype evaluation,muscle histopathology and ultrastructure in a Duchenne muscular dystrophy animal model     

LI Zhong  ZHANG Cheng  CHEN Guo-jun  LIU Xiao-rong 《园艺学报》2004,20(3):311-316

AIM: To evaluate the genotype , muscle histopathology and ultrastructure in dko mice. METHODS: Dystrophin/Utrophin-deficient double knockouts (dko) mice were obtained from university of Oxford, UK. Genotype of filial generation of heterozygote was evaluated by PCR-SSP. HE staining and fluorescent immunohistochemistry by SABC-Cy3 were used to detect striated muscle of dko mouse, and the muscle ultrastructure was observed by transmission electron microscope(TEM). RESULTS: In 112 filial generation mice, there were 28 mdx (25.0%), 26 dko (23.2%) and 58 heterozygote (51.8%), which coincided with the law of Mendelian inheritance. HE staining showed that the myocytes were not very uniform, there were phenomenon of round outline, centrally nucleated fibers, widening interspace, inflammatory cell infiltration and connective tissue proliferation in dko mice. There were no any immunofluorescent expression of dystrophin and utrophin in sarcolemma in dko mice. TEM showed sarcolemma breakage, separation and edema, and loose myofibril texture, inflammatory cell infiltration and connective tissue proliferation in dko mice. CONCLUSION: PCR-SSP is a very quick and accurate way for genotype evaluation of filial generation. The pathophysiology of dko mouse was very similar to Duchenne muscular dystrophy (DMD), and dko mouse is an ideal animal model for study of DMD clinical therapy.  相似文献   

雏鸡饮服鸡源芽孢杆菌对其生长性能的影响   总被引：1，自引：0，他引：1  

李国治  李明丽  王米  任文辉 《水禽世界》2004,(4):14-15

本研究利用从健康鸡消化道分离的鸡源芽孢杆菌饲喂雏鸡,采用鸡白痢沙门氏菌作为攻毒病原菌,观察试验组与对照组在生长速度及死亡率上的差异。实验结果表明试验组的生长速度明显高于对照组(p<0.01),死亡率明显低于对照组(低20%),说明芽孢杆菌是一种用于研制微生态制剂的理想益生菌。  相似文献   

The　Brief　Introduction　to　the　Sino-US　Joint　Centers　for　Soil　and　Water　Conservation　and　Environmental　Protection     

LIJing LIRui ZHENGFen-li 《水土保持研究》2004,11(4):174-176

Erosion and transport of soil has worldwide implications for agriculture, landscape stability, climate, natural hazards, and clean, renewable resources of water and air. Assured access to clean water and a healthy and safe environment requires an ethic of conservation and protection. The minimum scale in which these principles apply successfully is basin wide. These are the fundamental concerns of the Sino-US Centers for Soil and Water Conservation and Environmental Protection.  相似文献   

结晶葡萄糖的生产工艺、用途及其发展前景   总被引：2，自引：0，他引：2  

权伍荣  张健  李森 《延边大学农学学报》2004,26(4):313-318

介绍了结晶葡萄糖的生产工艺、主要用途及其发展前景．  相似文献   

Mid-storage and pre-sowing seed treatments for lettuce and carrot     

Dolly Pan  R.N. Basu 《Scientia Horticulturae》1985,25(1):11-19

Mid-storage hydration-dehydration treatments with medium-vigour lettuce seed very effectively reduced physiological deterioration in storage and ultimately showed better field emergence and final yield. Soaking-drying treatment of high-vigour lettuce seed was not effective. Pre-sowing treatment of medium-vigour lettuce seed, though less effective than mid-storage treatment, gave better field performance, but high-vigour seed did not benefit from the treatment. For carrot, both mid-storage and pre-sowing seed treatments proved beneficial, with the former showing better performance than the latter. Unlike lettuce, well-preserved carrot seed responded positively to the pre-sowing treatment.  相似文献   

大蒜根蛆的发生规律及防治技术   总被引：2，自引：0，他引：2  

郑露  李国敬  关银池  刘绪红  易行荣  黄俊斌 《湖北农业科学》2008,47(8)

对湖北省当阳市大蒜根蛆的发生规律、不同品种的抗虫性及药剂防治进行了调查试验。结果表明，大蒜根蛆在湖北1年发生2代，第1代根蛆危害时期为9月8～26日，第2代为9月25日至10月20日，第2代根蛆发生量大，危害严重；不同大蒜品种根蛆危害差异不大；药剂防治以10％地友GR处理土壤防效较好，为88.8％，叶面喷雾以2.5％肯打EC防效较好，为77.7％。  相似文献   

不同稀释液对藏獒精液保存效果的影响     

贺生中  刘栋  罗有文  李群 《家畜生态学报》2009,30(6):72-75

比较了8种稀释液在藏獒精液冷冻保存和低、常温保存(5 ℃,10 ℃,15 ℃,20 ℃)的效果.结果表明:2号和8号稀释液在冷冻保存时效果最好,精子活力和顶体完整率分别达到0.40和49%,与对照组相比差异显著(P<0.05).在藏獒的低温、常温保存试验中,所有稀释液在15 ℃时保存时间最长,综合考虑,7号液保存效果最好;在15℃时精子平均存活时间为103 h,显著高于对照组和其它稀释液(P<0.05).  相似文献