超高效液相色谱法测定饲料中斑蝥黄和β-阿朴-8’-胡萝卜素酸乙酯含量方法的研究     

章厉劼  钱莘莘  孙真峥  王庆红  廖欢  赵贵 《中国饲料》2021,(3):78-82

为有效快速测定饲料中斑蝥黄和β-阿朴-8’-胡萝卜素酸乙酯含量,饲料经甲醇、正己烷和乙酸乙酯混合液萃取后,在氮气保护下浓缩,用甲基叔丁基醚-乙腈溶液复溶,注入超高效液相色谱仪中进行测定。结果表明:斑蝥黄浓度为1.25~10.0μg/mL,β-阿朴-8’-胡萝卜素酸乙酯为2.50~20.0μg/mL线性关系良好;斑蝥黄和β-阿朴-8’-胡萝卜素酸乙酯的检测限为0.06 mg/kg,定量限为0.20 mg/kg。斑蝥黄5个添加水平的平均回收率为72.8%~82.8%,相对标准偏差(RSD)为0.41%~1.06%;β-阿朴-8’-胡萝卜素酸乙酯5个添加水平的平均回收率为72.0%~82.8%,RSD为0.46%~1.41%。说明该方法简便、快速、灵敏度高、准确度好,适用于测定饲料中斑蝥黄和β-阿朴-8’-胡萝卜素酸乙酯含量。  相似文献   

2011-2020年夏季全国草原净初级生产力时空变化及其气象影响评估     

孙应龙  延昊  赵晓凤  陈紫璇  曹云 《草地学报》2021,29(4):848-854

为评估2011—2020年夏季全国草地净初级生产力时空变化以及气象条件对净初级生产力的影响,本研究依据气象站逐日气象观测资料以及中分辨率成像光谱(Moderate-resolution imaging spectroradiometer,MODIS)系列卫星资料遥感数据,分析了近10年夏季全国草地净初级生产力变化。结果表明:2011—2020年夏季全国草原净初级生产力总体呈现增加趋势,平均每年增加0.2 gC·m^-2·a^-1,内蒙古中部、甘肃东部、青海东部、西藏东部等地近10年草原净初级生产力明显增加。近10年全国大部草原区平均气温和降水量均呈增加趋势,较好的气象条件利于夏季牧草生长。2020年夏季全国草原区的气温和降水量也较常年偏高,北方草原区草地平均净初级生产力为121.3 gC·m^-2·夏季^-1,为近10年偏好年份。2011—2020年夏季全国草原净初级生产力呈增加趋势,较好的气象条件对其贡献较大。  相似文献   

Cloning and Bioinformatic Analysis of L1 Gene of BPV from Guizhou Province     

ZHANG Hai  ZHOU Bi-jun  YANG Zhong-cheng  LIAO Mei  WANG Kai-gong  WEN Ming  CHENG Zhen-tao  WANG Wei  FENG Xu-fang 《中国畜牧兽医》2016,43(11):2834-2843

In order to study and analyze L1 gene of bovine papillomavirus（BPV）in Guizhou province,the L1 gene of BPV-GZ01 strain was amplified,cloned and sequenced using bioinformatic softwares and methods,and the secondary structure,tertiary structure,B-cell preponderant epitope,conserved domains analysis, transmembrane domain and signal peptide of L1 gene were predicted.The results showed that the length of L1 gene was 1 494 bp,encoding 497 amino acids.The L1 gene of BPV-GZ01 strain shared an amino acid identities of 98.6%,99.4%,98.4%,94.4% and 91.3%,and a nucleotide identities of 99.1%,99.8%,99.4%,87.6% and 82.8% with those of BPV2,BPV2-SW01,BPV2-AKS01,BPV13 and BPV1 strains,respectively.The results of phylogenetic tree analysis indicated that there was a close relationship between BPV-GZ01 and BPV2-SW01 strains.The prediction of secondary structure of L1 protein indicated that the random coil,extended strand and alphahelix took a higher percentage.The L1 protein was supposed contain 6 potential antigen epitopes.And no transmembrane domains and no signal peptide were found.The tertiary structure of L1 protein was curved spiral structure.These results provided a theoretical basis for immunologic diagnosis and further research of nucleic acid vaccine of BPV.  相似文献   

褪黑素的生物合成及对繁殖性能的影响     

张钰  邓学梅  廖晨星  刘国世 《中国乳业》2023,(2):3-9

褪黑素是一种广泛存在于动物体内的激素,不仅能够调节动物昼夜节律,还能够从多方面影响动物的繁殖性能,对于季节性繁殖动物,褪黑素根据日照时长的变化表现出抑制或促进繁殖性能的作用。对于幼年动物,褪黑素表现出抑制发情的作用,体成熟后则表现促进发情的作用。褪黑素主要通过下丘脑-垂体-性腺轴调控动物繁殖活动,除此之外,褪黑素还能够直接作用于生殖系统甚至早期胚胎,通过提高组织及细胞抗炎及抗氧化功能来改善动物繁殖性能。目前,褪黑素能够用于治疗精神疾病及炎症、促进畜禽繁殖和调节昼夜节律,是相关研究领域的热门话题。本文详细描述了褪黑素的生物合成以及分泌调控,并结合褪黑素的生理功能,阐述了对动物繁殖性能以及辅助生殖的影响。  相似文献   

牛奶中褪黑素含量的检测方法     

沈紫霞  常毅  刘巧香  郭刚  马慧  李有志  廖晨星  刘国世 《中国乳业》2023,(2):10-14

褪黑素作为一种多功能活性因子,在畜牧生产中具有十分重要的作用。近年来,有关天然高褪黑素奶的研究大规模开展,对奶中褪黑素含量测定需求也有所增加。目前比较常见的褪黑素检测方法有气相色谱法（GC）、气相色谱-质谱联用法（GC-MS）、高效液相色谱法（HPLC）、高效液相色谱-质谱联用法（LC-MS）、放射免疫测定法（RIA）、酶联免疫吸附测定法（ELISA）、紫外分光光度法（UV）。本文从原理、特点及优缺点等方面对这几种方法展开介绍,并对各方法的应用进行简单的比较,为后续相关研究与应用提供一定参考。  相似文献   

突发性环境污染事故应急监测的速度保障     

张先宝  曹锋  廖志凌 《农机化研究》2004,(3):144-145

针对突发性环境事故如何保证应急监测速度的问题，从应急监测前准备工作及应急监测运行期两方面进行说明，为保障应急监测速度提供了切实可行的方法和步骤。  相似文献   

Automatic Measuring Method of Eyeglass Lens Power     

YANG Bing-shang  LIAO Hai-yang 《保鲜与加工》2006,(10):65-68

A method of measuring eyeglass lens power is proposed. A position detector and 3 beams of ray are used to measure the prismatic, spherical and astigmatic powers. Based on the method, an automatic focimeter with simple structure can be developed. The measure errors of prismatic and spherical powers are analyzed, from which it is concluded that the precision of the position detector is the key to the method.  相似文献   

Moving-Out-Inventory Planning for Steel Plate Products     

LIAO Xiao _Yun  LIU Yan _Ling  WANG Yi _Liang 《保鲜与加工》2002,(1):146-149

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Experimental Study of Multifunctional Acid to Deliquesce Stratum-jam     

LIU Ju-mei  WANG Hong-tu  LIAO Jiu-ming  LI Wen-hu  LIU Yuan 《保鲜与加工》2006,(2):120-123127

Aiming at the oneness of acid to deliquesce stratum-jam applied in scene at the present time,which is incapable of deliquescing complex dirty simultaneously,this article confects a new multifunctional O/W acid.This acid is an emulsified mixture containing 15% hydrochloric acid as its extro-posture,high dissolved oil as its internal state under the function of some proper exterior active reagent.It is composed of 0.5%OP-10,0.5%Span80,0.2%Tween80,0.06%imidazole and 0.01%twelve-alcohol,and the content of oil is equal to the acid content.It can be indicated that this acid has good ability of resolving mineral(CaCO_3,MgCO_3) and organic compound(mainly colloidal bitumen) when it was poured into the stratum,so that it can deliquesce oleic stratum-jam,resume and improve the penetrability of stratum,boost the ability of liquid flowing up.  相似文献   

Dropwise Condensation Heat Transfer Coefficient on the Horizontal Circular Surface with Radial Gradient Surface Energy     

GU Yang-biao  LIAO Qiang  ZHU Xun  WANG Hong 《保鲜与加工》2006,(5):48-51

A theoretical model is developed for the dropwise condensation heat transfer on the horizontal circular surface with radial gradient surface energy based on the heat transfer model of individual condensate drop and the size distribution model of condensate drop on homogeneous condensation surface.The effect of variation of contact angle on the gradient surface on condensation heat transfer and condensate drop size distribution is taken account of in this model.The theoretical calculation method was obtained to predict the dropwise condensation heat transfer coefficient on the horizontal circular surface with radial gradient surface energy under various wall subcooled temperature,contact angle profile on wall surface,and working fluid.The effects of surface energy gradient,wall subcooled temperature,and thermophysical properties of condensate on the condensation heat transfer are discussed respectively.The calculation results show that the condensation heat transfer coefficient increases slightly with the increase of wall subcooled temperature.As latent heat and surface tension increasing,the condensation heat transfer coefficient increases.A larger surface energy gradient induces a larger condensation heat transfer coefficient.  相似文献