草原生态奖补政策实施效应——基于政治社会学视角的实证分析     

路冠军  刘永功 《干旱区资源与环境》2015,(7):29-32

草原生态奖补政策有助于保护草原生态,促进畜牧业的发展转型,有利于民族地区的和谐稳定。生态奖补政策的实施对牧民生计、基层社会治理等方面也产生了一些负面影响。未来的政策实践过程中,需要建立政策评估反馈机制,不断提高生态奖补标准,完善奖补资金发放办法,着力调动地方政府积极性,力求实现奖补手段的多样化,不断提高政策的法制化、系统化水平。  相似文献   

Effect Comparison of Adenovirus Vector Transfer into Different Buffalo Cells     

DUAN An-qin  PANG Chun-ying  ZHU Peng  LU Xing-rong  DENG Ting-xian  LIANG Xian-wei 《中国畜牧兽医》2016,43(10):2661-2665

Buffalo mammary epithelial cell,cumulus cell and fibroblasts were transfected by adenovirus vectors and compared their transfection efficiency.293 cells were transfected with pBHGloxdelE13cre and pDC316-eGFP by liposome,the virus was collected and titer was detected.Buffalo mammary epithelial cell,cumulus cell and fibroblasts were exposed to different multiplicity of infection (MOI) of adenovirus vectors.After 72 h,the cells were observed with inverted fluorescence microscope,and transfection efficiency was calculated.When the MOI was 25,50,100,200 and 400,the transfection efficiency of fibroblasts were 0.7%,7.0%,9.0%,12.5% and 34.0%,the transfection efficiency of cumulus cells were 42.5%,55.3%,57.4%,76.0% and 80.0%,the transfection efficiency of mammary epithelial cells were 88.7%,100%,100%,100% and 100%.The results showed that the transfection efficiency of mammary epithelial cell was the best,followed by cumulus cell,and fibroblast was poor.  相似文献   

Effects of Different Nitrogen Treatments on N use Efficiency and Grain Yield of Mechanically-transplanted Hybrid Rice     

Chang YE  Ke LIU  Shaowen LIU  Jian LU  Mengtao GAO  Bilin LU  Yunbo ZHANG 《农业科学与技术》2017,18(2)

In order to investigate effects of topdressing on grain yield and N use efficiency of mechanically-transplanted hybrid rice,this experiment was conducted with 2-line hybrid rice cultivars,Quanliangyou 1 and Quanliangyou 681 with 3 application levels of N fertilizer,No,N1,N2,respectively.The results showed that there were no significant differences in grain yields of the 2 varieties between treatments N1 and N2.It was indicated that N is not the factor which could limit grain yield within limited application levels of N fertilizer.High N fertilizer treatment could contribute to the formation of effective panicles and spikelets per panicle,but it could also lead to the decline in grain filling rate.It was suggested that lowering the amount of Nfertilizer could facilitate improvement of N-use efficiency.  相似文献   

Cloning and Sequence Analysis of Buffalo Keap1 Gene and Investigation of Its Expression Pattern in Different Tissues     

ZHAO Xin  RUAN Zi-yun  GUO Zhen-wei  ZHOU Wen-ting  QIN Xi-ling  NIU Xiang-li  LU Feng-hua  SHI De-shun 《中国畜牧兽医》2016,43(8):1975-1982

In this study,the CDS sequence of buffalo Keap1 gene was cloned and analyzed,then its expression pattern in different tissues was also investigated.A pair of primers of buffalo Keap1 gene was designed based on the nucleotide sequence of Bos taurus Keap1 gene from GenBank,and then the buffalo Keap1 gene was amplified.Using the bioinformation techniques,the gene sequence and the protein structure were analyzed.The expression level of Keap1 gene in different tissues were detected with Real-time quantitative PCR.The results showed that the length of buffalo Keap1 gene coding sequence was 1 875 bp and encoded 624 amino acids.The multiple sequence alignment results showed that buffalo Keap1 gene shared 99%,96%,92% and 90% of similar nucleotide sequence with that of Bos taurus,Ovis aries,Sus scrofa and Homo sapiens,respectively.And the phyogenetic tree also showed the conservatism between several different species.The second structure of buffalo Keap1 protein was predicted as 24 alpha regions,40 beta regions,38 turn regions and 27 coil regions.In addition,the results of Real-time quantitative PCR showed that Keap1 mRNA exists in all seven tissues,but the most abundant expression was in heart and the minimal expression was in liver and spleen.The results provided an foundation for further study of Keap1-Nrf2-ARE signal pathway,for enhancing the ability of antioxidant of buffalo embryo in vitro culture.  相似文献   

江西省新型农业社会化服务体系的特点与运行模式     

薛刚  卢琨  李玉萍 《热带作物学报》2016,(3):66-68

实地调研江西省新型农业社会化服务体系建设现状，对江西省目前的新型农业社会化服务体系的特点与运行模式进行分析，针对当地在服务体系构建过程中出现的问题，提出新时期的发展建议。  相似文献   

不同药剂组合对马铃薯晚疫病的防治效果     

韩小女  展康  蔡永占  何彩花  吕庆长  杨亚琼 《中国马铃薯》2020,(1):46-52

马铃薯晚疫病是马铃薯生产中的重要病害,是影响马铃薯产量和品质的重要因素之一,在云南春作马铃薯上普遍发生,且危害较重,给当地马铃薯产业带来了巨大的经济损失。为减轻马铃薯晚疫病对马铃薯生产造成的损失,试验研究了11种药剂7种药剂组合(包衣剂+保护剂+治疗剂)通过播种期种薯包衣,现蕾期叶面喷施一次保护剂,发病初期和发病中期叶面各喷一次治疗剂的方法防治马铃薯晚疫病。结果表明,药剂组合种薯包衣+甲霜·锰锌+氟吡菌胺·霜霉威的防治效果最好,防治效果达到60.99%,增产率176.28%,增效率152.27%;其次是种薯包衣+霜脲·锰锌+氟吡菌胺·霜霉威组合,防治效果达到60.15%,增产率173.20%,增效率150.55%。这2个药剂组合对马铃薯晚疫病均有极显著的防治效果,增产增效明显。  相似文献   

鹌鹑ESR1基因的外显子8多态性与早期生长性能的关联分析     

樊红灯  白俊艳  付学言  曹恒  杨帅  庞有志  时坤鹏  卢小宁  马永康  陈梦柯  董智豪  安肖凯  常世增  侯伟晓  巩慧荣 《黑龙江畜牧兽医》2020,(2)

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土壤温度对作物根区环境及玉米根冠生长的调控研究进展     

夏镇卿  路海东 《玉米科学》2020,28(3):99-104

土壤温度对作物的生长发育具有很强的调控作用。在全球气候变化背景下,根区土壤温度对玉米生长的影响及其调控机制已成为当前研究的一个热点。结合目前农业生产中土壤低温和高温对玉米造成危害的现状,从土壤温度对作物根区土壤环境以及玉米根系生长、冠层发育与产量形成的影响方面,综述土壤温度对玉米生长发育调控作用的研究进展,介绍改变土壤温度的措施以及对玉米土壤温度研究的发展方向进行展望,为今后的研究提供参考。  相似文献   

基因组Ⅰ型和Ⅴ型传染性胰脏坏死病毒的分离与鉴定     

段凯越  赵景壮  任广明  邵轶智  卢彤岩  何保全  徐黎明 《大连海洋大学学报》2021,36(5):736-744

为调查造成中国西北某虹鳟Oncorhynchus mykiss养殖场幼鱼出现持续性死亡的病因,采用组织病理切片观察及分子鉴定的方法对患病虹鳟进行了鲑鳟常见病毒筛查,同时采用系统发育分析、电镜观察、滴度测定及人工回归感染等方法对分离获得的毒株进行了基因型分析、显微结构观察及毒力特性分析等研究.结果表明:患病虹鳟的肝、脾及肾组织细胞发生广泛性坏死,并伴随空泡化及溶解等现象;由病鱼内脏组织制备的组织匀浆悬液均能够感染CHSE-214细胞并产生典型细胞病变(CPE);从该养殖场的不同养殖区域共分离到3株传染性胰脏坏死病毒(infectious pancreatic necrosis virus,IPNV),其中IPNV-F1和IPNV-W2属于基因组Ⅰ型,分别与IPNV中国分离株WZ2016(KX355401)和ChRtm213(KX234591)同源性最高,VP2基因片段序列一致性分别为100％和98.5％,IPNV-W1属于基因组Ⅴ型,与意大利毒株IPNV/O.mykiss/I/PN/208/Mar88(MG543567)同源性最高,二者的VP2基因片段序列一致性为99.1％;接种病毒后的CHSE-214细胞内存在大量呈晶格状排列的无囊膜病毒粒子,3株IPNV分离株在CHSE-214细胞上的平均滴度分别为107.43 TCID50/0.1 mL(IPNV-F1)、107.30 TCID50/0.1 mL(IPNV-W1)和107.29 TCID50/0.1 mL(IPNV-W2);分别以0.1 mL/尾的剂量向健康虹鳟幼鱼腹腔注射各IPNV分离株,在攻毒后60 d内虹鳟未出现死亡,攻毒后30 d时,病毒在组织中的平均滴度分别为104.50 TCID50/0.1 g组织(IPNV-F1)、105.38 TCID50/0.1 g组织(IPNV-W1)和104.13 TCID50/0.1 g组织(IPNV-W2),攻毒后60 d时,病毒在组织中的平均滴度下降为103.43 TCID50/0.1 g组织(IPNV-F1)、104.50 TCID50/0.1 g组织(IPNV-W1)和103.21 TCID50/0.1 g组织(IPNV-W2).研究表明,该发病养殖场同时存在基因组Ⅰ型和Ⅴ型的IPNV,本研究首次从同一养殖场分离到两种基因型的IPNV,可为中国传染性胰脏坏死病(IPN)的防控提供参考.  相似文献   

自动施肥系统开发与性能测试     

李明仁  芦阳  李东燕  张万罡  马庆安  贺正  刘扬  马富裕 《新疆农业科学》2021,58(12):2228-2235

目的 开发一种适应于以固态水溶肥为原料的自动施肥系统,测试分析自动施肥系统性能。方法 主控机采用ARM9电路控制模块可实现对轮灌编组、预搅拌时长、施肥开始与结束时间、施肥持续时长、施肥量等参数的设置;选择以蠕动泵为注肥装置,通过变频器控制注肥泵电机功率的方式控制注肥速率,控制施肥量。对装置核心部件搅拌器额定功率、计量方式、溶肥搅拌参数、排肥速度及固液相比例等主要参数等进行设计与测试。结果 电感脉冲计量方式标准误差最大值1.26%,误差小、性价比好,确定其为本装置采用的计量方式;搅拌器以1.5 Kw额定功率、38 r/min转速搅拌、肥液浓度在1.1~1.3 g/mL、预搅拌时间30 min时,罐内各液位输出肥液浓度值差异不显著(P< 0.05),达到对肥料浓度均匀性的设计要求。结论 将施肥开始前的预搅拌时间设为30 min、搅拌转速设为38 r/min、肥液浓度不高于1.3 g/mL,输出肥液浓度有较好的均匀性,实现精准施肥。  相似文献