Pharmacodynamic study of a long-acting parenteral formulation of omeprazole in horses     

Téllez E  Ocampo L  Bernad M  Sumano H 《Journal of veterinary pharmacology and therapeutics》2005,28(6):587-589

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Melengestrol acetate as a tool for inducing early ovulation in transitional mares     

López-Bayghen C  Zozaya H  Ocampo L  Brumbaugh GW  Sumano H 《Acta veterinaria Hungarica》2008,56(1):125-131

The efficacy of melengestrol acetate (MGA) to shorten the vernal transition of mares by synchronising and accelerating the first ovulation of the year after 60 days of phototherapy was determined by ultrasonographic monitoring. Sixteen mares in late transition were fed two doses of MGA (150 mg/mare/day and 100 mg/mare/day, respectively) for 10 days. A luteolytic dose of prostaglandin was administered to each mare one day after the end of MGA treatment. The presence and duration of oestrus, follicular growth, uterine oedema and presence of ovulation were monitored by ultrasonography and the cervical tone was evaluated by rectal palpation. Ovulation was detected in 87.5% of the mares treated with 150 mg MGA/mare/day for 10 days, and in 62.5% of the mares receiving 100 mg MGA/mare/day for 10 days. This was statistically different (P = 0.03) from the untreated control mares having an ovulation rate of 20%. Mares that received 150 mg MGA/day for 10 days had a mean treatment to ovulation interval of 13.1 +/- 5.97 days after the end of treatment, while mares that received 100 mg MGA/day for 10 days had a mean of 25.6 +/- 10.50 days (P = 0.01) to ovulation. These results suggest that MGA can be used for synchronising and hastening the first ovulation of the year in mares.  相似文献   

Interspecific hybridization between cultivated and wild species of genus Passiflora L.     

J. Ocampo  J. C. Arias  R. Urrea 《Euphytica》2016,209(2):395-408

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Tracing the origin of Spanish common bean cultivars using biochemical and molecular markers     

César?Humberto?Ocampo  Juan?Pedro?Martín  María?Dolores?Sánchez-YélamoEmail author  Jesús?María?Ortiz  Orlando?Toro 《Genetic Resources and Crop Evolution》2005,52(1):33-40

Fifty-four accessions of Phaseolus vulgaris cultivated in Spain and representing a broad variability for this country, were studied together with 30 samples of wild forms of American origin. Two reference cultivars (from the Andes and Mesoamerica) plus two outgroups (P. coccineus and Vigna unguiculata) were also included. RAPD analysis of DNA leaf extracts were carried out with four selected primers. We also studied morphological characters of the seeds and the phaseolin electrophoretic patterns. Multivariate analysis with the UPGMA method using RAPD data clustered the samples in four groups and, comparing with morphological data and phaseolin types, showed that the Spanish cultivars were mainly of Andean origin. Nevertheless, occurrence of introgression in Spain and the consideration of the country as a second center of variability for beans can also explain the obtained results.  相似文献   

Possible influence of Rhizobium on VA mycorrhiza metabolic activity in double symbiosis of alfalfa plants (Medicago sativa L.) grown in a pot experiment     

D. Piccini  J. A. Ocampo  E. J. Bedmar 《Biology and Fertility of Soils》1988,6(1):65-67

Summary Alfalfa (Medicago sativa, L. cv Aragón) plants were grown under greenhouse conditions in pots of inert sand and vermiculite. The plants were inoculated with Rhizobium meliloti strain 102F28, with Glomus fasciculatus or with a mixture of both microorganisms. Plants inoculated with both Rhizobium and Glomus had the highest shoot dry weight and the lowest root-to-shoot ratio. Roots from dually inoculated plants also had a higher oxygen uptake and nodule nitrogenase activity than those from plants inoculated with either of the two microsymbionts alone. However, the dry weight of the roots from only VAM-infected plants was higher than those from Rhizobium or from Rhizobium plus Glomus-inoculated ones. These differences did not correlate with succinate dehydrogenase activity, which was similar between treatments. Nutrient element concentrations were increased in dually infected plants in comparison with those of plants inoculated with only Rhizobium or Glomus. These data suggest that Rhizobium may affect fungal metabolism and that the effect is not achieved via the tricarboxylic acid pathway.  相似文献   

Isozyme polymorphism provides fingerprints for germplasm ofArachis glabrata Bentham     

Brigitte L. Maass  César H. Ocampo 《Genetic Resources and Crop Evolution》1995,42(1):77-82

Arachis glabrata Bentham, a wild, perennial relative of the cultivated peanut, has been under development as a forage plant for the subtropics and tropics since the early 1960s. To complement the limited genetic resources available in those years, a significant germplasm collection has been assembled in recent years. But little is known about the genetic diversity in this species because research has focused mainly on agronomy. This study aimed to investigate the utility of isozymes for characterisingA. glabrata germplasm. Polyacrylamide gel electrophoresis (PAGE) was applied to rhizome-tip tissue. Isozyme characterisation by PAGE showed a high degree of intraspecific polymorphism for the isozymes α-EST, ACP, GOT, and DIA. When analysed together, the four isozyme systems could clearly distinguish all 15 accessions ofA. glabrata held in the germplasm collection at CIAT. Although several accessions originated from the same region, similarity in isozyme patterns was not correlated with geographic origin. Examining rhizome-tip tissue is useful inA. glabrata, a species which produces few seeds.  相似文献   

Contribution of Arbuscular Mycorrhizal and Saprobe Fungi to the Aluminum Resistance of Eucalyptus globulus     

C. A. Arriagada  M. A. Herrera  F. Borie  J. A. Ocampo 《Water, air, and soil pollution》2007,182(1-4):383-394

Aluminum in acidic conditions is toxic to plants. Aluminum tolerance in some plant species has been ascribed to arbuscular mycorrhizal fungal symbiosis. In this study, the application of aluminum was found to inhibit mycelia development of saprobe fungi Fusarium concolor and Trichoderma koningii and the hyphal length of the arbuscular mycorrhizal fungi Glomus mosseae and Glomus deserticola in vitro. Several levels of aluminum were applied to Eucalyptus globulus plants and inoculated with arbuscular mycorrhizal fungi alone or together with both saprobe fungi. The application of 1,500 mg kg^?1 decreased the shoot and root dry weight, chlorophyll content and total P, Mg, and Ca concentrations in the shoot of E. globulus. However, both mycorrhizal fungi G. mosseae and G. deserticola inoculated alone increased the shoot dry weight of Eucalyptus, compared with a non- arbuscular mycorrhizal inoculated control treated with 1,500 mg kg^?1 of aluminum. When 1,500 mg kg^?1 of aluminum was applied, T. koningii increased the effect of G. deserticola on the shoot weight of eucalyptus, whereas with 3,000 mg kg^?1, shoot weight and arbuscular mycorrhizal colonization decreased in all treatments. With 1,500 mg kg^?1, the highest accumulation of aluminum in the shoot was obtained when G. deserticola was inoculated together with T. koningii. The possibility of manipulating an arbuscular mycorrhizal inoculation together with a saprobe fungus confers a high aluminum resistance in E. globulus. The effect of such combined inoculation is particularly important in some Chilean volcanic acid soils, mainly those which have been intensively cropped and are without lime addition, which facilitates the increase of phytotoxic aluminum species and limits their agricultural use. Therefore, such dual inoculation in field conditions deserves further investigation. Overall, the arbuscular mycorrhizal and saprobe fungi contribute to the increase in resistance of E. globulus to aluminium.  相似文献   

Timing of sequential changes in chromosome configurations during the 1st meiotic division of pig oocytes cultured in vitro     

M B Ocampo  L T Ocampo  H Kanagawa 《The Japanese journal of veterinary research》1990,38(3-4):127-137

This study examines the timing of changes in chromosome configurations of pig oocytes derived from small antral follicles of follicular and/or inactive stage donors using modified 199 medium supplemented with gonadotropins (Follicle Stimulating Hormone (FSH), 10 IU/ml; Human Chorionic Gonadotropin (hCG), 10 IU/ml) and Glucosamine (0.539 mg/ml). Oocytes (n = 1,215) were fixed at the end of 3 hourly intervals from 0-48 hr of culture. Results were expressed as the percentage of oocytes at each stage of maturation for each time point. The germinal vesicle (GV) stage was observed for the first 17.6 hr; germinal vesicle breakdown (GVBD) stage between 17.6-26.4 hr; metaphase I (M-I) from 26.4-30.9 hr; anaphase I (A-I) ranged from 30.9-33.4 hr; telophase I (T-I) at 33.4-34.4 hr; and metaphase II (M-II) at 34.4-48 hr.  相似文献   

Digestive protease activity in juvenile Farfantepenaeus californiensis as a function of dissolved oxygen and temperature   总被引：1，自引：0，他引：1  

Lucía Ocampo  & J Marina Ezquerra 《Aquaculture Research》2002,33(13):1073-1080

Total protease, trypsin and chymotrypsin activities were examined in postlarval Farfantepenaeus californiensis (Holmes) after they had been reared for 50 days at two dissolved oxygen concentrations (5.8 and 2.6 mg L⁻¹) and three temperatures (19, 23 and 27 °C). Three replicated experiments were performed with a 12-h light/dark photoperiod. Two-way analysis of variance indicated a significant effect of temperature and oxygen (P < 0.05) on total protease and chymotrypsin activities, but no effect of oxygen was found on trypsin activity. A tendency towards increased protease, trypsin and chymotrypsin activities with acclimation temperature was observed. Total protease activity (units per mg protein) varied from 0.26 in shrimp held at 19 °C and low oxygen concentration to 1.41 in shrimp held at 23 °C and high oxygen concentration. Trypsin activity (units per mg protein) varied from 0.16 in shrimp held at 19 °C and low oxygen concentration to 0.86 in shrimp held at 27 °C and high oxygen concentration. Chymotrypsin activity (units per mg protein) varied from 0.014 in shrimp held at 19 °C and low oxygen concentration to 0.15 (units per mg protein) in shrimp held at 27 °C and low oxygen concentration. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) zymograms of hepatopancreas from each of the treatment groups showed numerous bands. The results suggest that different digestive protease enzymes arise as an adaptation mechanism to temperature and dissolved oxygen variations at this particular stage of life.  相似文献   

Characterizing the Saltol Quantitative Trait Locus for Salinity Tolerance in Rice   总被引：2，自引：0，他引：2  

Michael J. Thomson  Marjorie de Ocampo  James Egdane  M. Akhlasur Rahman  Andres Godwin Sajise  Dante L. Adorada  Ellen Tumimbang-Raiz  Eduardo Blumwald  Zeba I. Seraj  Rakesh K. Singh  Glenn B. Gregorio  Abdelbagi M. Ismail 《Rice》2010,3(2-3):148-160

This study characterized Pokkali-derived quantitative trait loci (QTLs) for seedling stage salinity tolerance in preparation for use in marker-assisted breeding. An analysis of 100 SSR markers on 140 IR29/Pokkali recombinant inbred lines (RILs) confirmed the location of the Saltol QTL on chromosome 1 and identified additional QTLs associated with tolerance. Analysis of a series of backcross lines and near-isogenic lines (NILs) developed to better characterize the effect of the Saltol locus revealed that Saltol mainly acted to control shoot Na⁺/K⁺ homeostasis. Multiple QTLs were required to acquire a high level of tolerance. Unexpectedly, multiple Pokkali alleles at Saltol were detected within the RIL population and between backcross lines, and representative lines were compared with seven Pokkali accessions to better characterize this allelic variation. Thus, while the Saltol locus presents a complex scenario, it provides an opportunity for marker-assisted backcrossing to improve salt tolerance of popular varieties followed by targeting multiple loci through QTL pyramiding for areas with higher salt stress.  相似文献