An integrative study of Sakia sisanganensis n. sp. (Rhabditida: Tylenchidae) from Sisangan forest,Iran, and new morphological observations for the genus     

Yousef Panahandeh  Mohammad Reza Atighi  Irma Tandingan De Ley  Ebrahim Pourjam  Manuel Mundo‐Ocampo  Joaquín Abolafia  Davoud Koolivand  Farahnaz Jahanshahi Afshar  Majid Pedram 《Forest Pathology》2019,49(5)

Further data on the morphology (the lip region characters) and phylogeny of the genus Sakia are presented. The new observations were based on scanning electron microscopy (SEM) and light microscopy (LM). A new species, Sakia sisanganensis n. sp., was recovered from rotten wood of a dead beech tree (Fagus orientalis) in northern Iran, herein described and illustrated based on an integrative approach, that is morphological, morphometric and molecular characters. The new species is characterized by a combination of the following features: fine transverse striae and vestigial single band in the lateral field in SEM. Labial area dorso‐ventrally flattened. Oral region with two concentric hexagonal plates, the inner one apparently elevated. Amphidial openings short, slit‐like. Stylet delicate. Median bulb fusiform to spindle‐shaped with weak valvular apparatus. Spermatheca functional. Tail filiform with faintly pointed tip and males common. The new species was morphologically compared with four known species of the genus, viz., S. alii, S. arboris, S. castori and S. indica, all having indistinct lateral fields. Molecular phylogenetic analyses were performed based on the small subunit ribosomal RNA gene (SSU rDNA). In the Bayesian inference (BI), S. sisanganensis n. sp. with two isolates was strongly supported as a sister taxon of a clade harbouring S. arboris + Lelenchus species. However, in the maximum likelihood (ML) analysis, the new species formed a clade with S. arboris, thus supporting the reciprocal monophyly of the genera Sakia and Lelenchus. Accordingly, the test of monophyly was performed (using Bayes factor) and the results did not reject the monophyly of sakia (i.e., S. sisanganensis n. sp. and S. arboris as sister taxa) based on the currently available data.  相似文献   

Bacteriostasis and the inoculation of phosphate-solubilizing bacteria in the rhizosphere     

J.A. Ocampo  J.M. Barea  E. Montoya 《Soil biology & biochemistry》1978,10(5):439-440

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Hydrolytic enzyme activities in maize (Zea mays) and sorghum (Sorghum bicolor) roots inoculated with Gluconacetobacter diazotrophicus and Glomus intraradices     

M.L. Adriano-Anaya  J.A. Ocampo  I. García-Romera 《Soil biology & biochemistry》2006,38(5):879-886

Two strains of Gluconacetobacter diazotrophicus (Pal 5, UAP5541) and the arbuscular mycorrhizal fungus Glomus intraradices increased both the shoot and root dry weight of sorghum 45 days after inoculation, whereas they had no effect on the shoot and root dry weight of maize. Co-inoculation (Gluconacetobacter diazotrophicus plus Glomus mosseae) did not increase the shoot and root dry weight of either plant. There was a synergistic effect of Gluconacetobacter diazotrophicus on root colonization of maize by Glomus intraradices, whereas an antagonistic interaction was observed in the sorghum root where the number of Gluconacetobacter diazotrophicus and the colonization by Glomus intraradices were reduced. Plant roots inoculated with Gluconacetobacter diazotrophicus and Glomus intraradices, either separately or together, significantly increased root endoglucanase, endopolymethylgalacturonase and endoxyloglucanase activities. The increase varied according to the plant. For example, in comparison with non-inoculated plants, there were higher endoglucanase (+328%), endopolymethylgalacturonase (+180%) and endoxyloglucanase (+125%) activities in 45-day old co-inoculated maize, but not in 45-day old sorghum. The possibility is discussed that hydrolytic enzyme activities were increased as a result of inoculation with Gluconacetobacter diazotrophicus, considering this to be one of the mechanisms by which these bacteria may increase root colonization by AM fungi.  相似文献   

Isozyme variation in Passiflora subgenera Tacsonia and Manicata. Relationships between cultivated and wild species     

Sergio D. Segura  Geo Coppens d'Eeckenbrugge  César H. Ocampo  Patrick Ollitrault 《Genetic Resources and Crop Evolution》2003,50(4):417-427

Isozyme variation was studied in 87 plants from 32 cultivated and wild accessions of banana passion fruit (P. tripartita var. mollissima, P. tripartita var. tripartita, P. tarminiana, and P. mixta), rosy passion fruit (P. cumbalensis), tin-tin (P. pinnatistipula), gulián (P. ampullacea), P. antioquensis, P. bracteosa, and P. manicata, from the Andes of Venezuela, Colombia, and Ecuador. Six polymorphic enzyme systems (IDH, PGDH, PGM, DIA, PRX, and ACP) revealed 31 zymotypes characterized by the presence or absence of 31 electromorphs. Cluster analysis separated clearly the accessions of P. tarminiana, P. tripartita, P. mixta, and P. cumbalensis from the less typical species of subgenus Tacsonia, which is consistent with morphological evidence. P. mixta showed the highest intraspecific variation and the closest affinity with P. tripartita. The accessions of these two species formed two clusters, one dominated by Colombian genotypes and the other dominated by Ecuadorian genotypes. One of the P. tripartita var. mollissima accessions clustered close to P. tarminiana accessions. The affinity between these three species is particularly interesting for conservation and use of banana passion fruit genetic resources. All the other species formed monospecific clusters.  相似文献   

Interaction between Alternaria alternata or Fusarium equiseti and Glomus mosseae and its effects on plant growth     

C. B. McAllister  J. M. Garcia-Garrido  I. Garcia-Romera  A. Godeas  J. A. Ocampo 《Biology and Fertility of Soils》1997,24(3):301-305

The effect of inoculation with the saprophytic fungi Alternaria alternata or Fusarium equiseti on maize (Zea mays) and lettuce (Lactuca sativa) with or without arbuscular mycorrhizal (AM) colonization by Glomus mosseae was studied in a greenhouse trial. Plant dry weights of non-AM-inoculated maize and lettuce were unaffected by the presence of A. alternata and F. equiseti. In contrast, A. alternata and F. equiseti decreased plant dry weights and mycorrhization when inoculated to the rhizosphere before G. mosseae. The saprophytic fungi inoculated 2 weeks after G. mosseae did not affect the percentage of root length colonized by the AM endophyte, but did affect its metabolic activity assessed as succinate dehydrogenase activity. Although F. equiseti inoculated at the same time as G. mosseae did not affect mycorrhization of maize roots, its effect on AM colonization of lettuce roots was similar to that with A. alternata. In the rhizosphere of both plants, the population of saprophytic fungi decreased significantly, but was not affected by the presence of G. mosseae. Our results suggest that there may have been a direct effect of the saprophytic fungi on the mycorrhizal fungi in the extramatrical phase of the latter, and when the AM fungus was established in the root the AM fungus was less affected by the saprophytic fungi. Received: 16 January 1996  相似文献   

Contribution of Arbuscular Mycorrhizal and Saprobe Fungi to the Tolerance of Eucalyptus globulus to Pb     

C. A. Arriagada  M. A. Herrera  J. A. Ocampo 《Water, air, and soil pollution》2005,166(1-4):31-47

The application of Pb inhibited the development of mycelia of the saprobe fungi Fusarium concolor and Trichoderma koningii and the hyphal length of the arbuscular mycorrhizal fungi (AM) Glomus mosseae and G. deserticola in vitro. The application to soil of 1500 mg kg^?1 of Pb decreases the dry weight, total N, P, Mg and Fe concentration and chlorophyll content of the shoot of E. globulus no inoculated with AM fungi. However, G. deserticola increased the dry weight, total nutrient concentration and chlorophyll content of the shoot, and the percentage of AM root length colonization and the succinate dehydrogenase activity of AM mycelia of E. globulus in presence of 1500 mg kg^?1 of Pb, and these increases were higher when G. deserticola was inoculated together with T. koningii. The application to soil of 3000 mg kg^?1 of Pb decreased the shoot dry weight and AM colonization of E. globulus in all treatments tested. Pb was accumulated in the stem more than in the leaves of E. globulus. In presence of 1500 mg kg^?1 of Pb the highest accumulation of this metal in the stem took place when E. globulus was colonized with G. deserticola. In conclusion, the possibility to increase Lead accumulation in stem is very attractive for phytoextraction function, the saprobe fungi, AM and their interaction may have a potential role in elevating phytoextraction efficiency and stimulate plant growth under adverse conditions such as lead contaminated soil.  相似文献   

Superovulation response of upgraded indigenous Philippine goat (Capra hircus)     

M B Ocampo  R S Uenishi  C A Valdez  Z Muralla  M Hishinuma  H Kanagawa 《The Japanese journal of veterinary research》1988,36(3-4):249-255

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Aqueous biphasic system to extract arbuscular mycorrhizal spores from soils     

M. Salvador-Figueroa  L. Adriano-Anaya  S. Tzusuki-Caldern  M.E. Gavito Pardo  J.A. Ocampo 《Soil biology & biochemistry》2008,40(9):2477

The aqueous biphasic system (ABS) method using PEG and ammonium sulphate improved the recovery of spores from tropical soils as compared with the sucrose gradient method. The ABS was not affected by low temperatures. The optimal recovery of spores was achieved when 100% PEG and 50% ammonium sulphate concentration and PEG₈₀₀₀ instead of PEG₄₀₀₀ were used. Under these conditions an optimal interface formation which facilitates the localization and recovery of spores was obtained. Both phases of the ABS are aqueous and did not affect either the viability of spores or the level of root colonization reached by these spores. Soil composition did not affect the number of spores obtained by the ABS method either.  相似文献   

Genetic analysis seedling vigour under osmotic stress in rice by QTL mapping     

Atefeh Sabouri  Hossein Sabouri  Marjorie De Ocampo 《Russian Agricultural Sciences》2012,38(5-6):423-429

Seed vigour is one of the most important parameters for establishing rice seed that affects high yielding production, especially under stress condition in rice (Oryza sativa L.). Improvement of rice yields under abiotic stress through breeding requires a good understanding of genetic factors controlling important traits such as seedling vigour and related traits. To understand the genetic background of the relationships among these traits in indicia rice, we genetically analyzed F2:3 population derived from a cross between Tarommahalli × Khazar, under osmotic stress caused by sorbitol. In this investigation, a simple sequence repeat (SSR) linkage map of 1231.50 cM of rice was constructed by using 74 markers and quantitative trait loci (QTL) analysis was accomplished for eight traits including germination percent, seed germination rate, radicle length, plumule length, coleoptile length, radicle dry matter, plumule dry matter, and coleoptile dry matter. A total of 32 QTLs were identified for the eight traits. Among them, one QTL for percent of germination (qGP-8) was located in RM6208-8264 on chromosome 8 showed large effect (28.99%) on the trait. These results suggest the potential for increase productivity by transferring osmotic stress tolerance gene from Tarommahalli to other rice lines and identification of QTLs with large effects could be considered for use in marker assisted selection (MAS).  相似文献   

Surface protein components from entomopathogenic nematodes and their symbiotic bacteria: effects on immune responses of the greater wax moth,Galleria mellonella (Lepidoptera: Pyralidae)          下载免费PDF全文

Maurizio Francesco Brivio  Andrea Toscano  Simone Maria De Pasquale  Andrea De Lerma Barbaro  Stefano Giovannardi  Giovanna Finzi  Maristella Mastore 《Pest management science》2018,74(9):2089-2099

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