Observations on the Development of the Brassica Cyst Eelworm     

Serfiah  Lewis 《Plant pathology》1971,20(3):144-148

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Antigen expression in cultured oral keratinocytes from dogs     

J E Wilkinson  C A Smith  M M Suter  R M Lewis 《American journal of veterinary research》1991,52(3):445-448

Oral keratinocytes from dogs were cultured on either collagen gels or artificial matrices at the air-liquid interface, and the expression of keratinocyte antigens and basement membrane components was determined, using various monoclonal and polyclonal antibodies. Keratinocytes grown on collagen gels expressed pemphigus vulgaris, pemphigus foliaceous, and bullous pemphigoid antigens. Diffuse, suprabasal, and superficial keratinocyte membrane differentiation antigens identified by various monoclonal antibodies also were expressed in a pattern identical to that observed in the native tissue. Laminin and type-IV collagen were deposited at the keratinocyte-collagen interface in a patchy distribution. When synthetic matrices were used, the oral keratinocytes differentiated, but to a lesser extent than cells grown on collagen gels. Antigen expression for cells grown on synthetic matrices was similar to that for cells on collagen, except for failure of the keratinocytes on synthetic membranes to express superficial cell antigens and pemphigus foliaceous antigens.  相似文献   

Gastric mast cell tumor in a cougar     

H D Martin  D D Lewis  S L Lin  E R Jacobson 《Journal of the American Veterinary Medical Association》1985,187(11):1258-1260

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Spontaneous mesangiocapillary glomerulonephritis in Finn cross lambs from Alberta.          下载免费PDF全文

P F Frelier  J Pritchard  D L Armstrong  W T Nagge    R M Lewis 《Canadian journal of veterinary research》1984,48(2):215-218

A spontaneous mesangiocapillary glomerulonephritis occurred in three, one to three month old Finnish Landrace cross lambs from a flock in northern Alberta. The ram was a purebred Finn sheep, and the ewes were Finn-Rambouillet and Finn-Suffolk-Rambouillet crosses. The lambs were found dead without previous clinical signs. Histologically there was marked thickening of glomerular capillary basement membranes, proliferation of mesangial cells, and peri-glomerular fibrosis. An interstitial infiltration of plasma cells and lymphocytes was present with occasional tubular degeneration and proteinaceous cast formation. Focal leukoencephalomalacia was present in one lamb. Electron microscopy demonstrated deposition of electron-dense deposits in a subendothelial location with occasional fusion of overlying foot processes in glomerular capillaries. Indirect immunofluorescence studies demonstrated positive staining material in glomerular capillary walls. These findings in Finnish Landrace cross lambs are characteristic of mesangiocapillary glomerulonephritis, a condition heretofore not reported in North America.  相似文献   

Effect of dietary energy intake during lactation on performance of primiparous sows and their litters     

J L Nelssen  A J Lewis  E R Peo  J D Crenshaw 《Journal of animal science》1985,61(5):1164-1171

A total of 146 primiparous sows was used in four replications of an experiment to investigate the effect of energy intake during a 28-d lactation on sow and litter performance. Dietary treatments consisted of three energy intakes; 10, 12 or 14 Mcal of metabolizable energy (ME) X sow-1 X d-1. All sows were fed equal amounts of crude protein, vitamins and minerals daily, which met or exceeded standard recommendations. The experiment was initiated at parturition. Sow weight and backfat loss during lactation decreased linearly (P less than .001) as energy intake increased. There were no differences in litter size at either 14 d of lactation or weaning. Pig weights on d 14 increased linearly (P less than .05) and litter weights tended to increase linearly (P = .13) as energy intake increased. At weaning, pig weights and litter weights increased (P less than .05) as sow energy intake increased. There were no significant differences in the percentages of sows in estrus by 7, 14, 21 and 70 d postweaning, but sows fed 10 Mcal ME/d had a slightly longer interval from weaning to first estrus than sows fed higher energy intakes. Serum urea concentrations of sows were inversely related to energy intake during lactation. Serum creatinine concentrations were not affected by energy intake. An intake of 10 Mcal ME/d by primiparous sows during a 28-d lactation resulted in reduced sow and litter performance; there was little difference between sows fed 12 and 14 Mcal ME/d.  相似文献   

Weaning weight response of pigs to simple and complex diets     

L V Himmelberg  E R Peo  A J Lewis  J D Crenshaw 《Journal of animal science》1985,61(1):18-26

Five experiments were conducted to determine (1) the value of simple vs complex diets on gains and feed conversion of pigs weaned at a constant age but varying in weight, (2) the optimum postweaning time to change from a complex to a simple diet and (3) the effect of previous treatment on subsequent performance. In the first experiment, pigs were weaned at 3 wk +/- 3 d and assigned within four weight groups (4.1, 5.0, 5.6 and 6.7 kg) to a simple or complex diet that was fed for 28 d. The complex diet improved (P less than .01) pig performance in all weight groups. As anticipated, the heavier pigs (5.6 and 6.7 kg) ate more (P less than .02) and grew faster (P less than .03) than did their lighter weight littermates (4.1 and 5.0 kg). The second experiment involved pigs weaned at 3 wk +/- 3 d that were assigned within two weight groups (4.4 and 6.6 kg) and fed a complex diet for 10 d, after which one-half of each weight group was switched to a simple diet. Pigs fed the complex diet for the entire 24-d period gained faster (P less than .05) and were more efficient (P less than .05) than those changed to the simple diet after 10 d. The heavier weight (6.6 kg) pigs ate more (P less than .02) and gained faster (P less than .02) than did the lighter weight (4.4 kg) pigs.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Effects of long-term estrogen implants in beef heifers     

Kniffen DM  Wagner WR  Lewis PE 《Journal of animal science》1999,77(11):2886-2892

Crossbred beef heifers (n = 78) were assigned randomly to one of three treatments. Heifers received either no implant, one estradiol-releasing implant (Compudose), or two estradiol-releasing implants. Heifers were implanted at birth and then reimplanted every 150 d. Calves were maintained with the cows until weaning at approximately 200 d of age. Heifers were placed in the feedlot as one group and fed a growing diet for 56 d. Following the growing phase, heifers were segregated into their respective treatment groups and fed until selected by industry buyers for harvest. Beginning at 1 yr of age and continuing every 14 d until puberty or harvest, heifers were palpated per rectum, and blood samples were collected for determination of ovarian activity and attainment of puberty. Serum progesterone of > or =1 ng/mL and(or) palpation of a detectable corpus luteum were criteria of puberty. At weaning and again at harvest, an x-ray was taken of the left front leg of six heifers selected randomly from each group. Metacarpal bones III and IV from the same animals were collected at harvest and transected for determination of epiphyseal plate closure. The x-ray scores and the actual measurements had a correlation of .94. Epiphyseal plate closure occurred in a dose-related manner, with heifers on the higher dose of estrogen having earlier plate closure than heifers on the lower dose. At harvest, reproductive tissues and carcass data were collected for all heifers. Eighteen of 25 untreated control heifers (P < .05), 6 of 26 heifers treated with one implant, and 2 of 26 heifers treated with two implants attained puberty by the end of the experiment. No differences (P > .10) were detected among treatment groups for carcass traits. These data suggest that early and continuous treatment of heifers with estradiol implants can retard reproductive function.  相似文献   

An evaluation of the efficacy of injectable microencapsulated vitamin B12 in increasing and maintaining the serum and liver vitamin B12 concentrations of lambs     

Grace ND  Lewis DH 《New Zealand veterinary journal》1999,47(1):3-7

AIM: To develop a long-acting Vitamin B12 injection to prevent Co deficiency in sheep. METHODS: Formulations of microencapsulated Vitamin B12 in lactide-glycolide polymers were injected intramuscularly or subcutaneously into the anterior neck region of groups of 10 lambs and their efficacy determined from changes in serum and liver Vitamin B12 concentrations. RESULTS: The 95:5 lactide glycolide and the 100 lactide formulations containing more than 12.5% Vitamin B12 w/w significantly increased and maintained serum Vitamin B12 concentrations for at least 210 days as well as liver Vitamin B12 concentrations in treated lambs when compared with untreated controls. CONCLUSIONS: Injections of microencapsulated Vitamin B12 in lactide/glycolide copolymers are able to increase and maintain the Vitamin B12 status of lambs for at least 210 days. CLINICAL SIGNIFICANCE: Another option for the prevention of Co deficiency in sheep is now available using a long acting injectable Vitamin B12.  相似文献   

Declining risk from beef on the bone     

Lewis W 《The Veterinary record》1999,144(1):28

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Could treatment of pregnant mares prevent abortions due to equine piroplasmosis?     

Lewis BD  Penzhorn BL  Volkmann DH 《Journal of the South African Veterinary Association》1999,70(2):90-91

Treatment of pregnant mares to prevent abortions due to equine piroplasmosis is a novel idea practised empirically at some Thoroughbred studs in South Africa. This paper presents the results of an investigation to ascertain whether imidocarb dipropionate crosses the equine placenta. Three pregnant mares were injected intramuscularly with imidocarb and their foetuses were mechanically aborted at varying time intervals thereafter. Imidocarb was found in foetal blood at a level similar to that in the dam's blood, suggesting that imidocarb administered to the dam would be available for anti-parasitic activity in the foetal circulation. Uncertainty concerning the time of treatment to achieve the desired effect currently makes this a questionable exercise.  相似文献