谷氨酰胺对肉仔鸡脾组织结构发育的影响   总被引：2，自引：1，他引：2  

李升和  张承飞  吴亮  王珏  顾有方  许万祥  金光明 《畜牧与兽医》2004,36(10):5-8

160只 1日龄艾维茵肉仔鸡随机分成 4组 ,分别饲喂添加 0 % ,0 2 % ,0 4%和 0 8%谷氨酰胺的饲粮 2 8d。每周末每组取 6只鸡 ,颈静脉放血致死 ,取脾脏 ,Bouin液固定 ,制作石蜡切片 ,HE染色 ,光镜观察 ,显微摄影 ,研究基础日粮中添加谷氨酰胺对肉仔鸡脾组织结构的影响。结果显示 :添加谷氨酰胺的各试验组脾小结增多、增大 ;动脉周围淋巴鞘增厚 ;椭球增多、增大 ,细胞排列疏松 ,鞘毛细血管内皮细胞增高、增多 ;红髓比例降低 ,脾索内浆细胞和巨噬细胞增多。添加 0 8%谷氨酰胺的脾小结变化明显 ,添加 0 4%谷氨酰胺的动脉周围淋巴鞘和椭球变化明显。说明日粮中适量添加谷氨酰胺可促进脾内淋巴细胞的增殖与分化 ,从组织学角度证明 ,谷氨酰胺可促进脾的免疫应答能力 ,提高机体的免疫功能  相似文献   

斯氏线虫对进境原木上长林小蠹的防治试验   总被引：1，自引：0，他引：1  

胡学难  吴佳教  梁帆  梁广勤  叶伟峰  沈文涛 《植物检疫》2004,18(5):269-272

本文报道了斯氏线虫对进境原木上长林小蠹的生物防治试验.结果表明,斯氏线虫对长林小蠹的侵染力较强.通过毒力测定,斯氏线虫对长林小蠹3龄幼虫的LD50、LD99分别为21、432(条/头),而对蛹的LD50、LD99分别为12、230(条/头).在寄生强度测定中,斯氏线虫与长林小蠹3龄幼虫仅接触0.5h,每虫就可被侵入1.6条线虫,12h寄生的死亡率达到90%,24h寄生的死亡率为100%.斯氏线虫对长林小蠹表现出较高的致病性和寄生性.斯氏线虫在室内以浸泡法和淋施法防治长林小蠹的效果较好.  相似文献   

桃树不同生长型及其研究进展   总被引：7，自引：4，他引：7  

牛良  王志强  刘淑娥  宋银花  宗学普 《果树学报》2004,21(4):354-359

生长型是桃树重要的树体特征,是控制树体大小的遗传因素,与栽培关系密切。综合有关文献介绍了桃树的紧凑型、半矮化型、矮化型、柱型、直立型、短枝型、垂枝型等生长型资源及不同生长型在遗传、形态特征、生理、栽培等方面的研究进展。紧凑型受单位点显性基因控制,树体开张,分枝角度大,萌芽率及成枝力均高,光透过率较低;半矮化型树体介于普通型和紧凑型之间;矮化型为单基因隐性性状,节间极短,树体矮小,叶片密度大,光透过率低;柱型枝条分枝角度小,冠幅小,叶面积指数大;直立型介于柱型和普通型之间;短枝型能形成较多的短果枝,并以短果枝结果为主;垂枝型枝条披散下垂,枝条弯曲。不同生长型之间在氮素、干物质分配和内源激素水平上也存在一定的差异。紧凑型、矮化型由于树冠密度大,光透过率低而在应用上受到限制,直立型和柱型因适应了高密度栽培而更受人们的重视。针对目前存在的生长型分类与命名问题,作者认为应首先考虑节间长度,其次为萌芽率、成枝力等,然后考虑的是枝条的分枝角度。  相似文献   

芒果叶片生化特性与炭疽病发生的关系   总被引：1，自引：0，他引：1  

蒲金基  王向社  梁活华  刘晓妹 《果树学报》2004,21(5):434-437

研究了芒果叶片生化特性与炭疽病发生之间的关系。田间自然发病调查和室内人工接种鉴定结果均表明,随着芒果叶片由最初的古铜色向淡绿色和深绿色叶片的逐渐成熟老化,抗病性逐渐增强。对芒果叶片表面蜡质层和叶内可溶性糖、游离氨基酸、总酚含量及细胞膜相对透性的测定结果表明,与淡绿色叶片和老叶相比较,古铜色叶片含有较少的表面蜡质、叶内可溶性糖和总酚含量及较多的游离氨基酸,推断古铜色叶片对病害的易感性可能与其叶面蜡质和叶内总酚含量较少、叶内可溶性糖和游离氨基酸的比值(C/N比)较低有关。  相似文献   

Rat mesenchymal stem cells differentiate into neuron-like cells induced by berberine in vitro     

XIANG Ping  LI Hai-biao 《园艺学报》2004,20(1):51-53

AIM: To investigate whether berberine can induce rat mesenchymal stem cells (MSCs) to differentiate into neuron -like cells in vitro. METHODS: MSCs were separated from young rat femurs marrow and expanded in culture medium. MSCs were induced to differentiate by berberine. The morphological changes of MSCs were evaluated by light microscope.Neuron-spcific enolase (NSE), neurofilament (NF), glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry. RESULTS: Induced by berberine for 8 hours,MSCs exhibited neurotype . The expression of NSE and NF in the neuron-like cells was positive, but the glial astrocyte marker GFAP didn't express. CONCLUSION: Berberine may induce adult rat MSCs to differentiate into neuron-like cells in vitro.  相似文献   

Relation between TNF-α,TNFR I expression and apoptosis in oral lichen planus     

SHEN Li-Jia  RUAN Ping  YIN Cao  XIE Si-ming  ZHAO Tong 《园艺学报》2004,20(9):1664-1667

AIM: To examine the expression and distribution of tumor necrosis factor-α (TNF-α), tumor necrosis factor receptor I (TNFR I) and apoptosis in oral lichen planus, and evaluate their roles and relation in the oral lichen. METHODS: Immunohistochemical technique and TUNEL were employed to study the expression of TNF-α, TNFR I and apoptosis in 50 cases of oral lichen planus and 10 normal oral mucosa specimens. RESULTS: Compared with the normal control group, TNF-α expression was upregulated in mononuclear cells in lamina propria and decreased in keratinocytes in oral lichen planus lesion (P<0.05). On the contrary, TNFR I expression was increased in keratinocytes and decreased in lamina propria in oral lichen planus lesion (P<0.05). The increased apoptosis index in keratinocytes and the decreased apoptosis index in lamina propria were found in oral lichen planus (P<0.05). CONCLUSION: The accelerated apoptosis of keratinocytes and the inhibition of lymphocytes apoptosis may contribute to the formation and progression of oral lichen planus.  相似文献   

Astrocyte proliferation in the rat hippocampus after middle cerebral artery occlusion     

ZHANG Liang  WANG Wei  RUAN Xu-zhong 《园艺学报》2004,20(9):1553-1556

AIM: To study rat astrocyte proliferation in ipsilateral hippocampus following focal cerebral ischemia. METHODS: Ischemia was induced by temporary middle cerebral artery occlusion (MCAO). In hippocampus of rats at 3, 7 and 30 days after MCAO, the numbers and anatomic distribution of glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry. The protein expression of GFAP and proliferating cell nuclear antigen (PCNA) in the ipsilateral hippocampus were analyzed by Western blot analysis. RESULTS: Astrocytes appeared hypertrophic, with increased process thickness and numbers at 7 days after MCAO, and the highest density of astrocytes were seen at 30 days in the CA1, CA2 regions of the ipsilateral hippocampus. Western blot analysis revealed that GFAP levels were normal at 3 days, but increased by 7 days and remained elevation at 30 days. Western blot analysis of PCNA protein also revealed identified upregulation PCNA at 3 days after MCAO and the expression peaked at 7 days. CONCLUSION: This study demonstrates that focal cerebral ischemia in the rat results in a rapid response, a process often referred to as reactive astrogliosis or glial scarring, from resident astrocytes of the ipsilateral hippocampus to the side of ischemia.  相似文献   

Osmolarity,cell volume and proliferation in nasopharyngeal carcinoma cells     

WANG Li-wei  CHEN Li-xin  MAO Jian-wen  ZHU Lin-yan  NIE Si-huai  ZHONG Ping  SUN Xue-rong  CAI Bo  LI Pan 《园艺学报》2004,20(8):1349-1352

AIM: To investigate the relationship between osmolarity, cell volume and cell proliferation in nasopharyngeal carcinoma cells. METHODS: MTT method was applied to detect the proliferation ability of the poorly-differentiated nasopharyngeal carcinoma cell (CNE-2Z) under various osmolarity conditions. The flow cytometry was used to analyse cell cycle distribution. Cell volume was obtained by the image analysis of living cells and cell viability was determined by the trypan blue assay. RESULTS: Cultivation of cells under the hypertonic conditions of 370 and 440 mOsmol/L increased cell volume by 8.7% and 27.8% and facilitated cell proliferation by 22.2% and 33.9%, respectively. However, hypotonic incubation of cells with osmolarity of 160 and 230 mOsmol/L decreased cell volume by 12.8% and 4.1% and inhibited cell proliferation by 34.0% and 15.6%, respectively. Cell volume was positively correlated with cell proliferation rate. Long-term cultivation of cells under anisotonic conditions did not significantly alter cell cycle distribution, but hypotonic cultivation decreased cell viability. CONCLUSION: Proliferation of nasopharyngeal carcinoma cells was closely correlated with the osmolarity of culture medium and cell volume. Hypotonic cultivation may inhibit cell proliferation by decreasing cell volume to facilitate cell death mechanisms.  相似文献   

Effects of component of some Chinese herbs on proliferation of human umbilical vein endothelial cells in vitro     

HE Xiu-juan  LI Ping  QIU Quan-ying  SHENG Xun  WANG Fang 《园艺学报》2004,20(5):832-835

AIM: To observe the effects of some component of Chinese herbs for external use on proliferation of human umbilical vein endothelial cells (HUVEC) and investigate the mechanism of promoting tissue repair. METHODS: The method of MTT was used to examine the effects of Rg1, Rh1, perlolyrine, cinnamyl aldehyde, muscone, astragaluspolysaccharin (APS), velver antler polypeptide (VAP) and soluble extract of boswellia carterii birdw (BCB) on proliferation of HUVEC. RESULTS: APS did not promote proliferation of HUVEC at 9.75 mg/L-2.5 g/L; Rh1 promoted proliferation of HUVEC at 1.94 mg/L-0.5 g/L (P<0.05 or P＜0.01), and Rg1 inhibited proliferation of HUVEC at 31 mg/L (P<0.05); VAP promoted proliferation of HUVEC at 1 mg/L-0.5 g/L with optimal dose of 10 mg/L (P<0.01), Cinnamyl aldehyde promoted proliferation of HUVEC at 2 g/L(P＜0. 05); Muscone and soluble extract of BCB inhibited proliferation of HUVEC at 1 g/L, 0.5-2.5 kg/L(P＜0. 01), respectively; Perlolyrine inhibited proliferation of HUVEC at 0.125 g/L-0.5 g/L(P＜0. 01). CONCLUSION: The external herbs for supplementing Qi and warming Yang can promote HUVEC proliferation and improve angiogenesis during tissue repair. The external herbs for promoting blood circulation and accelerating capillary movement may have influence upon other stages of tissue repair.  相似文献   

Effects of fosinopril,captopril and valsartan on the expressionof tissue factor in human monocytes     

ZHOU Hua  ZHANG Dai-fu  SHAN Jiang  SU Hai  NIU Hui-yan  WANG Juan  YU Ping  QI Wei-gang 《园艺学报》2004,20(12):2261-2264

AIM: To investigate the effects of angiotensin-converting enzyme inhibitors (ACEI), fosinopril, captopril and angiotensin II AT₁ antagonists, valsartan on tissue factor (TF) expression on monocytes induced by lipopolysaccharide (LPS). METHODS: Mononuclear leukocytes from normal delivered female umbilical veins were incubated with bacterial LPS in presence or absence of different ACE inhibitors .At the end of incubation, the cells were disrupted by 3 freeze-thaw cycles. TF procoagulant activity was assessed by a one-stage clotting assay. RT-PCR was used to check TF mRNA expression, and GAPDH mRNA was used for parallel assay. RESULTS and CONCLUSION: The results showed that increased expression of TF mRNA induced by LPS was inhibited by fosinopril, captopril and valsartan, respectively, and the procoagualant activity of monocytes was also reduced.  相似文献