全文获取类型
收费全文 | 48457篇 |
免费 | 2324篇 |
国内免费 | 5222篇 |
专业分类
林业 | 5898篇 |
农学 | 8061篇 |
基础科学 | 4587篇 |
8118篇 | |
综合类 | 13751篇 |
农作物 | 2453篇 |
水产渔业 | 1812篇 |
畜牧兽医 | 6502篇 |
园艺 | 1510篇 |
植物保护 | 3311篇 |
出版年
2024年 | 101篇 |
2023年 | 414篇 |
2022年 | 1196篇 |
2021年 | 1931篇 |
2020年 | 1685篇 |
2019年 | 1758篇 |
2018年 | 1187篇 |
2017年 | 1618篇 |
2016年 | 1628篇 |
2015年 | 2252篇 |
2014年 | 2067篇 |
2013年 | 2563篇 |
2012年 | 3008篇 |
2011年 | 3289篇 |
2010年 | 3082篇 |
2009年 | 2888篇 |
2008年 | 2672篇 |
2007年 | 2683篇 |
2006年 | 2623篇 |
2005年 | 2604篇 |
2004年 | 1157篇 |
2003年 | 963篇 |
2002年 | 790篇 |
2001年 | 821篇 |
2000年 | 1004篇 |
1999年 | 1335篇 |
1998年 | 1191篇 |
1997年 | 1007篇 |
1996年 | 939篇 |
1995年 | 867篇 |
1994年 | 865篇 |
1993年 | 770篇 |
1992年 | 710篇 |
1991年 | 582篇 |
1990年 | 484篇 |
1989年 | 390篇 |
1988年 | 260篇 |
1987年 | 186篇 |
1986年 | 92篇 |
1985年 | 74篇 |
1984年 | 51篇 |
1983年 | 38篇 |
1982年 | 36篇 |
1981年 | 21篇 |
1980年 | 25篇 |
1979年 | 10篇 |
1978年 | 12篇 |
1977年 | 9篇 |
1965年 | 9篇 |
1963年 | 9篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
991.
Competition of infectious hypodermal and haematopoietic necrosis virus (IHHNV) with white spot syndrome virus (WSSV) for binding to shrimp cellular membrane
下载免费PDF全文
![点击此处可从《Journal of fish diseases》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Infectious hypodermal and haematopoietic necrosis virus (IHHNV) and white spot syndrome virus (WSSV) are two widespread shrimp viruses. The interference of IHHNV on WSSV was the first reported case of viral interference that involved crustacean viruses and has been subsequently confirmed. However, the mechanisms underlying the induction of WSSV resistance through IHHNV infection are practically unknown. In this study, the interference mechanisms between IHHNV and WSSV were studied using a competitive ELISA. The binding of WSSV and IHHNV to cellular membrane of Litopenaeus vannamei was examined. The results suggested that there existed a mutual competition between IHHNV and WSSV for binding to receptors present on cellular membrane of L. vannamei and that the inhibitory effects of WSSV towards IHHNV were more distinct than those of IHHNV towards WSSV. 相似文献
992.
Physiological metabolism of Haliotis discus hannai Ino under different light qualities and cycles
下载免费PDF全文
![点击此处可从《Aquaculture Research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The goal of this experiment was to examine the effects of different light qualities (red, white, blue) and cycles (12L:12D, 8L:16D, 4L:20D, 0L:24D, 16L:8D) on the survival, growth, metabolism and antioxidant defence system of Haliotis discus hannai Ino. Under red and white light at 4L:20D, the body weight‐specific growth rate of abalones was significantly higher than that of the 0L:24D group (P < 0.05). At 16L:8D, succinate dehydrogenase activity decreased and lactate dehydrogenase and alanine aminotransferase activity and the lactic acid content significantly rose (P < 0.05) in the red and white light groups. Under blue light, reactive oxygen species and malondialdehyde content for each light cycle were significantly higher than those of the red and white groups (P < 0.05). Under blue, white and red light, total antioxidant capacity (T‐AOC), superoxide dismutase (SOD) activity, glutathione peroxidase (GPX) activity and reduced glutathione (GSH) content gradually rose with increased duration of light exposure. Under red light, significant differences in these parameters were detected between the 4L:20D and 16L:8D groups (P < 0.05). Under blue and white light at 16L:8D, values of T‐AOC, SOD, GPX activity and GSH content were significantly lower than those of the 12L:12D group (P < 0.05). Overall, these results indicate that red light at light cycles of 4L:20D and 8L:16D is optimal for the culture and production of H. discus hannai, as these conditions promoted food intake, growth and health maintenance as well as normal physiological metabolism of the organism. 相似文献
993.
本文阐述了养鳟业的经济意义和价值,并从养鳟用水和生产方式、苗种生产、食用鱼养成、养鳟饲料、海水养鳟,疾病防治等方面总结了养鳟基本技术,同时对我国养鳟历史作了回顾,提出了我国养鳟业的发展前景。 相似文献
994.
高寒鲤染色体核型分析 总被引:3,自引:2,他引:3
采用植物血球凝集素(PHA)体内注射,肾组织细胞短期培养,常规空气干燥法制备了高寒鲤染色体,核型分析结果是:高寒鲤染色体的核型公式为2n=100=28m+28sm+44stt,染色体臂数NF=156。 相似文献
995.
996.
A study was conducted to investigate the effect of dietary yeast polysaccharides on some hematologic parameters and intestinal morphology of channel catfish. Channel catfish were fed diets containing yeast polysaccharides at 0 (control), 0.1, 0.2, or 0.3?% for 7?weeks. Each diet was provided to 10 channel catfish specimens (5.82?±?0.13?g initial weight) replicated 3?times in individual 250?L fiberglass tanks. Some hematologic parameters, leukocyte phagocytic activity, and intestinal morphology were monitored. After 7?weeks of trial, 0.2?% yeast polysaccharides resulted in significantly higher (P?0.05) monocyte numbers. Furthermore, fish fed 0.2?% yeast polysaccharide diet had higher (P?0.05) phagocytic rate of leukocyte. And 0.3?% yeast polysaccharide enhanced (P?0.05) phagocytic index of leukocyte. Histological evaluation showed yeast polysaccharide supplementation increased the height of intestine fold (0.1, 0.2 and 0.3?%) and the thick of muscular layers (0.2?%) in intestine (P?0.05). In addition, 0.1 and 0.3?% yeast polysaccharide supplementation improved the number of goblet cells (P?0.05). The results of this trial indicate that yeast polysaccharides supplementation could affect blood monocytes, improve leukocytes phagocytic activity, and the development of intestine in channel catfish. 相似文献
997.
7种经济贝类生殖腺脂肪酸含量的研究 总被引:3,自引:1,他引:3
为了提高文蛤等经济贝类的稚贝成活率,对文蛤等7种经济贝类的生殖腺中脂肪及脂肪酸的含量进行了研究。试验结果表明:不同的贝类总脂肪含量不同,其中,缢蛏最高,可达25.48%,虾夷扇贝22.32%,文蛤21.27%,泥蚶19.89%,紫贻贝16.79%,杂色鲍15.75%,青蛤含量最低,只有14.88%。由于浙江沿海的缢蛏一般是在10~11月产卵、受精及完成胚胎发育,在这种低温条件下配子的成熟和受精卵发育需要大量的能量,缢蛏的脂肪含量为最高,精巢为11.02%(占所取样品干重),卵巢为14.46%。雌雄生殖腺的脂肪含量也不同,一般是雌性生殖腺脂肪含量明显高于雄性生殖腺。不饱和脂肪酸的含量大于饱和脂肪酸,其中,紫贻贝含量最高,为67.23%,文蛤次之67.19%,泥蚶第三64.27%,虾夷扇贝59.74%,缢蛏59.40%,青蛤最低,仅为57.51%。生殖腺的质量是繁殖成败的关键,其中脂质的积累是影响生殖腺发育的主要因素之一。 相似文献
998.
999.
1000.
Bermudagrass (Cynodon ssp.) germplasm is genetically diverse and widely distributed in the world. The study was conducted to identify and assess the molecular variation and relationship among 24 cultivars developed in China, Australia and the USA. Sequence-Related Amplified Polymorphism (SRAP) was applied to cultivars identification in this study for the first time. Thirty of the 90 SRAP primer combinations generated a total of 274 clearly bands encompassing 249 (91%) polymorphic. Each bermudagrass cultivar has its unique binary code and can be distinguished from the others. Three distinct clusters were obtained by unweighted pair-group method with arithmetic averages based on the polymorphic markers. Coefficients of genetic distance among the genotypes ranged from 0.57 to 0.97. The results demonstrated that SRAP marker is a stable molecular marker technique for the identification of bermudagrass cultivars and their genetic relationships. 相似文献