全文获取类型
收费全文 | 642篇 |
免费 | 30篇 |
国内免费 | 2篇 |
专业分类
林业 | 20篇 |
农学 | 4篇 |
基础科学 | 3篇 |
68篇 | |
综合类 | 98篇 |
农作物 | 13篇 |
水产渔业 | 14篇 |
畜牧兽医 | 426篇 |
园艺 | 3篇 |
植物保护 | 25篇 |
出版年
2022年 | 4篇 |
2020年 | 6篇 |
2019年 | 5篇 |
2018年 | 6篇 |
2017年 | 6篇 |
2016年 | 7篇 |
2015年 | 8篇 |
2014年 | 9篇 |
2013年 | 15篇 |
2012年 | 21篇 |
2011年 | 23篇 |
2010年 | 12篇 |
2009年 | 24篇 |
2008年 | 26篇 |
2007年 | 31篇 |
2006年 | 24篇 |
2005年 | 26篇 |
2004年 | 29篇 |
2003年 | 29篇 |
2002年 | 27篇 |
2001年 | 22篇 |
2000年 | 24篇 |
1999年 | 18篇 |
1998年 | 11篇 |
1997年 | 7篇 |
1996年 | 22篇 |
1995年 | 7篇 |
1994年 | 14篇 |
1993年 | 9篇 |
1992年 | 12篇 |
1991年 | 7篇 |
1990年 | 8篇 |
1989年 | 13篇 |
1988年 | 21篇 |
1987年 | 6篇 |
1986年 | 5篇 |
1985年 | 9篇 |
1984年 | 10篇 |
1983年 | 9篇 |
1980年 | 5篇 |
1979年 | 10篇 |
1976年 | 5篇 |
1973年 | 4篇 |
1971年 | 3篇 |
1969年 | 4篇 |
1968年 | 5篇 |
1967年 | 4篇 |
1963年 | 3篇 |
1962年 | 4篇 |
1926年 | 3篇 |
排序方式: 共有674条查询结果,搜索用时 15 毫秒
51.
Humbles AA Lloyd CM McMillan SJ Friend DS Xanthou G McKenna EE Ghiran S Gerard NP Yu C Orkin SH Gerard C 《Science (New York, N.Y.)》2004,305(5691):1776-1779
Features of chronic asthma include airway hyperresponsiveness, inflammatory infiltrates, and structural changes in the airways, termed remodeling. The contribution of eosinophils, cells associated with asthma and allergy, remains to be established. We show that in mice with a total ablation of the eosinophil lineage, increases in airway hyperresponsiveness and mucus secretion were similar to those observed in wild-type mice, but eosinophil-deficient mice were significantly protected from peribronchiolar collagen deposition and increases in airway smooth muscle. These data suggest that eosinophils contribute substantially to airway remodeling but are not obligatory for allergen-induced lung dysfunction, and support an important role for eosinophil-targeted therapies in chronic asthma. 相似文献
52.
Michael D. DeBacker John S. Heywood Lloyd W. Morrison 《Strength and Conditioning Journal》2011,64(3):301-308
Prairies exhibit a bimodal frequency distribution of patch occupancy with a few dominant species accounting for most of the primary production and plant biomass and numerous other species occurring infrequently over time and space. Consequently, management interest is often focused on the abundance of common species, and on the persistence of less common species. We used spatially nested arrays of various sized plots to simultaneously measure species’ persistence and abundance at Tallgrass Prairie National Preserve, Kansas. Larger plots captured more species, with 127 species identified at the 10-m scale. Smaller plots, however, provided useful frequency data for measuring the abundance of common species. We defined a plot frame size that delivers a frequency in a 20–50% target range as the optimal plot size, and the frequency of a species sampled at its optimal plot size is termed the optimized frequency. Optimized frequency was estimated for 27 common species for the years 2002–2006. Of the nine perennial grasses in this group, five exhibited significant interannual variation within the 5-yr period. Bouteloua curtipendula exhibited a significant linear change over the 5-yr period, decreasing in frequency over time. From year to year, changes in optimized frequency among the 27 common species varied in both their trajectory (i.e., the average change), and amplitude (i.e., the average magnitude of change). Between 2005 and 2006, the average change in optimized frequency was -9.1%, the only year with an overall declining trajectory. The declining trajectory between 2005 and 2006 was significantly different from the increasing trajectories observed between 2002 and 2003 and between 2004 and 2005. No significant differences were observed in amplitude. We offer these techniques for aggregating information from multiple spatial scales to characterize the amplitude and trajectory of community-wide changes as tools to help scientists convey the results of multiscale research projects to land managers. 相似文献
53.
Fuel loading information is important for prescribed fire planning, evaluating wildfire risk, and understanding fire effects in grassland. Yet fuel loads in grasslands often go unmeasured because of the time required to clip plots and process samples, as well as limited access or proximity to a drying oven. We tested the digital photography biomass estimation technique for measuring fuel load in grasslands in two national parks in the eastern Great Plains. The method consists of using percentage image obstruction, as determined by digital photography, to estimate vegetation biomass based on a linear transformation (i.e., regressing dry clipped weights against percent digital obstruction). We used the technique with some modification and measured digital obstruction at two sites at Wilson’s Creek National Battlefield, Missouri (WICR), and three sites at Tallgrass Prairie National Preserve, Kansas (TAPR). The method did not result in strong correlations at either of the two sites at WICR (Site 1: r2=0.02; Site 2: r2=0.32), but performed relatively well at TAPR (Site 1 [<1 yr since burn]: r2=0.82; Site 2 [2 yr since burn]: r2=0.57; Site 3 [1 yr since burn]: r2=0.88). Linear regressions for the three sites at TAPR did not differ in slope (P>0.05). In general, the denser the vegetation, the weaker the relationship between the vegetation biomass of clip plots and the percentage image obstruction of digital images. The digital photography technique may not be useful for estimating fuel loads in grasslands with relatively high biomass (>80 g · 0.1 m?2) or digital image obstruction >50%. Large amounts of litter may also potentially reduce the accuracy of the technique. 相似文献
54.
55.
Triterpene saponins from aerial and subterranean organs of Medicago truncatula cv. Jemalong A-17 were qualitatively profiled and quantified using reverse-phase HPLC with on-line photodiode array detection and electrospray-ionization mass spectrometry (HPLC/PDA/ESI/MS). Absolute quantifications were performed for 3-O-beta-D-glucopyranosyl-medicagenic acid and soyasaponin 1 (3-O-[alpha-L-rhamnopyranosyl(1-->2)-beta-D-galactopyranosyl(1-->2)-beta-D-glucuronopyranoside] soyasapogenol B), whereas relative quantifications were determined for 29 other saponins in root, stem, leaf, seedpod, and seed. Roots contained the greatest total amount of saponins followed by leaf and seed, respectively. The quantitative data also reveal the differential accumulation of triterpene saponins in the various organs of M. truncatula. Specifically, relatively higher quantities of medicagenic acid conjugates accumulated in leaf and seed, whereas relatively higher levels of soysapogenol conjugates were observed in root. The differential accumulation of specific triterpene saponins is suggestive of spatially differentiated biosynthesis and/or biological function. 相似文献
56.
Frydman A Weisshaus O Huhman DV Sumner LW Bar-Peled M Lewinsohn E Fluhr R Gressel J Eyal Y 《Journal of agricultural and food chemistry》2005,53(25):9708-9712
Neohesperidin dihydrochalcone (NHDC) is a seminatural, safe, low-calorie sweetener, bitterness blocker, and flavor enhancer with unique properties and applications for the food, beverage, pharmaceutical, and animal feed industries. Current production is limited by the availability of the substrate neohesperidin, a flavonoid that accumulates to significant levels only in the inedible bitter citrus species. We propose a process to convert hesperidin, a tasteless flavonoid extracted from orange peels that are abundant byproducts of the vast orange juice industry, into neohesperidin using metabolic engineering and biotransformation via three steps: (i) extraction of hesperidin from orange peels, (ii) hydrolysis of sugar moieties, and (iii) biotransformation of hesperidin hydrolysis products into neohesperidin. We overcame the current technological bottleneck in biotransformation of hesperidin hydrolysis products into neohesperidin using metabolically engineered plant cell cultures expressing a recombinant flavanone-7-O-glucoside-2-O-rhamnosyltransferase. A small-scale production experiment established the feasibility of the proposed process. 相似文献
57.
Lloyd R. Stark William R. Wenerick Frederick M. Williams S. Edward Stevens Jr. Paul J. Wuest 《Water, air, and soil pollution》1994,75(3-4):405-420
Four volumes of spent mushroom compost were exposed to synthetic coal mine drainage (pH 3.5, 48 mg L?1 Fe, 22 mg L?1 Mn) under oxidizing conditions (Eh 300 to 400 mV) at a relatively high rate of flow. After 15 days, the compost lost its ability to elevate pH, to lower the redox potential, to lower outlet iron concentrations, and to lower manganese concentrations, with larger volumes retaining more Fe and H+, but less Mn. Estimated retention maxima per liter of spent mushroom compost were 281 μeq H+, 5.56 g Fe, and 0.15 g Mn. These values are similar to those reported elsewhere for peat. The ‘saturated’ compost was then mixed and exposed to mine water in order to eliminate ‘dead zones’ in the compost. Subsequently, the compost was re-exposed to synthetic mine water (pH 4.0, 60 mg L?1 Fe, O mg L?1 Mn) under a much lower flow rate and less oxidizing regime for a period of 114 days. Under the low flow regime, iron was first exported from the compost as reducing conditions were established, and then retained on a stable basis. In addition, Eh was lowered and pH was elevated by the compost. On a net basis, the capacity of the compost to retain iron was increased and apparently stable under the decreased flow conditions. 相似文献
58.
A sensitive bioassay method was developed using bidirectional diffusion of fungicides in agar bands instead of diffusion through 360°. This method permitted the measurement of fungicide movement through isolated cuticular disks into agar bands underneath. Oil dispersible formulations of oxycarboxin and pyracarbolid penetrated through cuticles of coffee much more efficiently than wettable powder formulations. 相似文献
59.
The lesions of a seasonal dermatitis in sheep occurred mainly on the ventral surface of the animals, but in other respects they resembled those of 'sweet itch' in horses. They were primarily an immediate hypersensitivity response and the circumstantial evidence indicated that they were due to the bites of Culicoides species, possibly C obsoletus. 相似文献
60.
David A. Barlow Ph.D. Judith M. Lloyd B.S. HT Paul Hellhake B.S. Jeffrey A. Seder J.D. M.B.A. 《Journal of Equine Veterinary Science》1984,4(2):60-66
Equine skeletal muscle was histologically and histochemically analyzed in orderto measure fiber composition. Well-sampled open muscle biopsies were obtained from the semitendinosus muscle in select yearling Thoroughbreds. Each biopsy was sectioned and stained for the Harris Hematoxylin and Eosin and the modified Gomori Trichrome in order to demonstrate the basic muscle fiber morphology delineating intra and extracellular elements. A thirdstain, CA++ activated myosin Adenosine Triphosphatase at pH 9.4 was used to differentiate between specific types on the basis of enzyme activities. Subsequent differentiation of Type I slow twitch and Type II fast twitch muscle fibers was assessed on the basisof staining intensity. The high predominance of Type II fast twitch fibers ranged from 85.1 to 100 % with a mean of 90.5 %. Racing records, as two- and three-year-olds, were monitored in order to qualitatively compare histochemical results with performance achievements. While caution must be exercised, results of this investigation suggested that it may be possible, at an early age to partially predict the potential of each horse to adapt to training and to compete successfully in races of varying distances. 相似文献