Hematology RIs for captive and wild Arabian Sand Gazelles (Gazella subgutturosa marica) of the Kingdom of Saudi Arabia          下载免费PDF全文

Gian Lorenzo D'Alterio  Chris Barichievy  William Macasero 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2018,47(2):213-217

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Vesicles Cytoplasmic Injection: An Efficient Technique to Produce Porcine Transgene‐Expressing Embryos          下载免费PDF全文

CG Luchetti  RJ Bevacqua  MS Lorenzo  MF Tello  M Willis  CP Buemo  DM Lombardo  DF Salamone 《Reproduction in domestic animals》2016,51(4):501-508

The use of vesicles co‐incubated with plasmids showed to improve the efficiency of cytoplasmic injection of transgenes in cattle. Here, this technique was tested as a simplified alternative for transgenes delivery in porcine zygotes. To this aim, cytoplasmic injection of the plasmid alone was compared to the injection with plasmids co‐incubated with vesicles both in diploid parthenogenic and IVF zygotes. The plasmid pcx‐egfp was injected circular (CP) at 3, 30 and 300 ng/μl and linear (LP) at 30 ng/μl. The experimental groups using parthenogenetic zygotes were as follows: CP naked at 3 ng/μl (N = 105), 30 ng/μl (N = 95) and 300 ng/μl (N = 65); Sham (N = 105); control not injected (N = 223); LP naked at 30 ng/μl (N = 78); LP vesicles (N = 115) and Sham vesicles (N = 59). For IVF zygotes: LP naked (N = 44) LP vesicles (N = 94), Sham (N = 59) and control (N = 79). Cleavage, blastocyst and GFP+ rates were analysed by Fisher's test (p < 0.05). The parthenogenic CP naked group showed lower cleavage respect to control (p < 0.05). The highest concentration of plasmids to allow development to blastocyst stage was 30 ng/μl. There were no differences in DNA fragmentation between groups. The parthenogenic LP naked group resulted in high GFP rates (46%) and also allowed the production of GFP blastocysts (33%). The cytoplasmic injection with LP vesicles into parthenogenic zygotes allowed 100% GFP blastocysts. Injected IVF showed higher cleavage rates than control (p < 0.05). In IVF zygotes, only the use of vesicles produced GFP blastocysts. The use of vesicles co‐incubated with plasmids improves the transgene expression efficiency for cytoplasmic injection in porcine zygotes and constitutes a simple technique for easy delivery of plasmids.  相似文献   

Suspended sediment load during floods in a small stream of the Dolomites (northeastern Italy)   总被引：3，自引：0，他引：3  

Mario A. Lenzi  Lorenzo Marchi   《CATENA》2000,39(4):15

Suspended sediment load was analyzed in a small, high-gradient stream of the Eastern Italian Alps which was instrumented to measure discharge and sediment transport. The ratio of suspended to total sediment yield and the relations between sediment concentration and water discharge were analyzed for seven floods which occurred from 1991 to 1996 in summer and autumn (from June to October). Different patterns of hysteresis in the relation between suspended sediment and discharge were related to types and locations of active sediment sources. The within-storm variation of particle size of suspended sediment during a major flood indicates a coarsening of transported material for increasing discharge. An analysis of grain size has shown that erosion areas on hillslopes were the main sources of suspended load.  相似文献   

IPM thresholds for Agriotes wireworm species in maize in Southern Europe     

Lorenzo Furlan 《Journal of pest science》2014,87(4):609-617

Currently, integrated pest management (IPM) of wireworms is not widespread in Europe. Therefore, to estimate the densities of three major wireworm species in southern Europe (Agriotes brevis Candeze, A. sordidus Illiger, and A. ustulatus Schäller), bait traps were deployed pre-seeding in maize fields in north-eastern Italy between 1993 and 2011. Research discovered that there was a significant correlation between all three wireworm species caught in the bait traps and damage to maize plants, but damage symptoms varied. Wherever A. ustulatus was the main species caught, there was no significant damage to maize plants, but seeds were damaged. Most of the symptoms caused by A. brevis and A. sordidus were to the central leaf/leaves, which wilted because of feeding on the collar. A. brevis was the most harmful species; when more than one A. brevis wireworm was caught per trap, plant damage sometimes resulted in reduced yield. Five A. ustulatus larvae per trap caused the same damage to maize as one A. brevis. A. sordidus came second (threshold two larvae/trap). These thresholds are reliable for: (1) bare soil in which there are no alternative food sources; (2) average soil temperature 10 cm beneath the surface of above 8 °C for 10 days; (3) soil humidity near to field water capacity, but days of flooding have not been considered. The implementation of the practical method described herein may lead to effective IPM of wireworms in maize and to a significant reduction in the number of fields treated with soil insecticides.  相似文献   

Transarticular Fixation With Cortical Screws Combined With Dorsal Laminectomy and Partial Discectomy as Surgical Treatment of Degenerative Lumbosacral Stenosis in 17 Dogs: Clinical and Computed Tomography Follow‐Up     

Lorenzo Golini DVM  MSc  Fraser I. Lewis PhD  Frank Steffen PD  Dr. Med. Vet. Diplomate ECVN 《Veterinary surgery : VS》2014,43(4):405-413

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An integrated web system to support veterinary activities in Italy for the management of information in epidemic emergencies     

S. Iannetti  L. Savini  D. Palma  P. Calistri  F. Natale  A. Di Lorenzo  A. Cerella  A. Giovannini 《Preventive veterinary medicine》2014

The management of public health emergencies is improved by quick, exhaustive and standardized flow of data on disease outbreaks, by using specific tools for data collection, registration and analysis. In this context, the National Information System for the Notification of Outbreaks of Animal Diseases (SIMAN) has been developed in Italy to collect and share data on the notifications of outbreaks of animal diseases. SIMAN is connected through web services to the national database of animals and holdings (BDN) and has been integrated with tools for the management of epidemic emergencies. The website has been updated with a section dedicated to the contingency planning in case of epidemic emergency. EpiTrace is one such useful tool also integrated in the BDN and based on the Social Network Analysis (SNA) and on network epidemiological models. This tool gives the possibility of assessing the risk associated to holdings and animals on the basis of their trade, in order to support the veterinary services in tracing back and forward the animals in case of outbreaks of infectious diseases.  相似文献   

Effects of seasonal grazing and precipitation regime on the soil macroinvertebrates of a Mediterranean old-field     

Alejandro Morón-Ríos  Miguel Á. Rodríguez  Lorenzo Pérez-Camacho  Salvador Rebollo 《European Journal of Soil Biology》2010,46(2):91-96

Soil macroinvertebrate communities (SMC) are well known to influence major ecosystem processes, but relatively few investigations have examined the mechanisms and factors involved in SMC regulation. We conducted a factorial experiment with combinations of seasonal grazing by sheep and irrigation (simulating different precipitation regimes) to assess their effects on the SMC of a semiarid Mediterranean old-field. We also analyzed effects on plant species richness, total aboveground biomass, and litter. The data were collected in autumn and spring, the two favorable seasons for SMC and primary production in the region, and season was included as an additional random factor. Main results were: 1) Ungrazed plots accumulated more aboveground plant biomass and litter during spring, providing extra food for soil biota. However, grazing during autumn or spring did not affect SMC characteristics. 2) Reduction of inter-annual precipitation variability in autumn and spring increased the abundance of two decomposer taxa: Oligochaeta and Diplopoda. Additionally, if summer drought was reduced, plant species richness, litter and the abundance of Isopoda were increased. 3) Oligochaeta and Diplopoda increase their abundance in spring, particularly, the most abundant taxon (Oligochaeta). We conclude that inter- and intra-annual variability in precipitation is a key environmental factor for the decomposer soil fauna in Mediterranean ecosystems, modifying the physical characteristics of the soils (humidity, hardness, etc.), as well as affecting the amount or characteristics of plant biomass or litter. The respiration system of the macroinvertebrates (cutaneous, tracheal or branquial) and the capacity to migrate vertically into the soil may determine the decomposers' responses to precipitation.  相似文献   

Prion protein in kidneys of scrapie-infected sheep     

Sisó S  González L  Jeffrey M  Martin S  Chianini F  Steele P 《The Veterinary record》2006,159(10):327-328

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Cytokine expression in porcine lungs experimentally infected with Mycoplasma hyopneumoniae   总被引：1，自引：0，他引：1  

Lorenzo H  Quesada O  Assunçao P  Castro A  Rodríguez F 《Veterinary immunology and immunopathology》2006,109(3-4):199-207

To gain further insight into the pathogenesis of porcine enzootic pneumonia (PEP), cytokine expression in different pulmonary compartments was examined. Mycoplasma hyopneumoniae (Mh) and proinflammatory and immunoregulatory cytokines (IL-1alpha, IL-1beta, IL-2, IL-4, IL-6, IL-8, IL-10 and TNF-alpha) were detected by immunohistochemical methods in porcine lungs experimentally infected with Mh. Ten pigs were inoculated intranasally with Mh and killed in pairs weekly from 1- to 5-week post-inoculation (wpi). Three Mh-free pigs were taken as controls. Mh-antigen was shown in paraffin-wax-embedded tissues by immunohistochemistry in the luminal surface of bronchial and bronchiolar epithelial cells of all Mh-infected pigs. Significant increase in cytokine expression was detected on snap-frozen tissues from the bronchoalveolar exudate of the airways, mononuclear cells of the alveolar septa and macrophages and lymphocytes of the peribronchial and peribronchiolar lymphoid tissue, from 1 wpi onwards, compared to expression in non-pneumonic lungs. The main cytokines in the BALT of Mh-infected animals that showed an increase were IL-2, IL-4, IL-8, IL-10 and TNF-alpha. In the alveolar septa and bronchoalveolar exudate IL-1 (alpha and beta), IL-2, IL-4, IL-8 and IL-10 expression also increased in infected animals.  相似文献   

Characteristics of magnetic resonance images of granulomatous meningoencephalomyelitis in 11 dogs     

Cherubini GB  Platt SR  Anderson TJ  Rusbridge C  Lorenzo V  Mantis P  Cappello R 《The Veterinary record》2006,159(4):110-115

The characteristics of magnetic resonance imaging (mri) of the brains and spinal cords of 11 dogs with histologically confirmed granulomatous meningoencephalomyelitis (gme) were determined. The lesions were in the brain of eight of the dogs, in the brain and spinal cord of two, and in the spinal cord alone in one dog. A single lesion was present in four of the dogs and multiple lesions were found in six. In one dog with intracranial signs, no visible lesions could be detected on mri. No meningeal enhancement was detected in T1-weighted images post-contrast, or in fluid attenuation inversion recovery (flair) images, but there were histological lesions in the meninges in nine of the dogs. The T2-weighted images and flair sequences were characterised in all cases by hyperintensity, whereas the signal intensity of the lesions on T1-weighted images was variable. After the administration of paramagnetic contrast, some of the lesions showed no enhancement, but others showed marked patterns of enhancement. The lesions in 10 of the dogs were easily identifiable by mri and the images had several unifying characteristics, but they could not be considered disease-specific.  相似文献