A Screening for the Occurrence of Autoreactive Antibodies in Sera of Pregnant and Non-pregnant Bitches     

S Schäfer-Somi  KR Herkner  S Neubauer  M Egerbacher  M Patzl  S Pollak  O Ali Aksoy  HB Beceriklisoy  H Kanca  M Findik  H Kalender  S Aslan 《Reproduction in domestic animals》2006,41(1):48-54

Sera of healthy pregnant (group I, n = 11) and non-pregnant (group II, n = 11) bitches were screened for autoantibodies (AAb). In both groups, blood samples were drawn every fifth day between days 5 and 55 after mating. Serum was analysed via indirect immunofluorescence (IIF) with the Canine ANA HEp-2 Screening Kit. In all animals, anticytoplasmic AAb were detected. Utilizing primate-heart substrate slides AAb against contractile proteins of the cytoplasm could be observed. The predominating fluorescence pattern in pregnant animals resembled above all desmin, which was proven via Western blot. The sera were then pre-incubated with tropomyosin, actin, vimentin, vinculin and keratin solutions, and assessed on HEp-2 slides and on human and canine fibroblasts as well. The latter substrate was used to verify whether the detected Ab were in fact AAb. Utilizing tropomyosin, revealed elimination of the cytoplasmic fluorescences on all three substrates. It is therefore assumed, that in sera of healthy dogs, AAb against contractile structure proteins of the cytoplasm are present regularly. The majority of pregnant bitches presented with higher end-point titres (EPT), than to be found in non-pregnant dogs. AAb against desmin played the key role in those patterns. In addition, sera were screened for thyroid specific AAb, namely thyroglobulin, thyroid peroxidase (TPO), T3 and T4, and for AAb against insulin by ELISA or Western blot (TPO). Only in two of the pregnant bitches a weak positive reaction (1:100) for T3-AAb was detected.  相似文献   

Calcified tumours of the paranasal sinuses in three horses     

Schaaf KL  Kannegieter NJ  Lovell DK 《Australian veterinary journal》2007,85(11):454-458

Three horses, a 10-year-old Thoroughbred mare, a 9-year-old Thoroughbred gelding and a 6-year-old Arab gelding, with calcified tumours of the paranasal sinuses, are described. All horses presented with purulent nasal discharges and facial distortion. Exophthalmos, blepharospasm and ocular discharge were also a feature in individual horses. A presumptive diagnosis of a calcified tumour was made on the basis of clinical signs and radiographic and endoscopic findings. The tumours ranged from 15 to 25 cm in diameter. A large frontonasal bone flap was used to expose the tumours, which were cleaved into several pieces with an osteotome and removed. Histological examination of the masses identified cementomas in two cases and an osteoma in the third. Long term follow up from 18 months to 5 years after surgery indicated that there was no recurrence. This case series demonstrates that, although calcified tumours of the paranasal sinuses are rare in horses, they should be considered in the differential diagnosis of purulent nasal discharge, facial swelling and ocular distortion, and are amenable to surgical treatment.  相似文献   

Phosphorus Budgets for Channel Catfish Ponds Receiving Diets with Different Phosphorus Concentrations   总被引：1，自引：0，他引：1  

Amit  Gross  Claude E.  Boyd  Richard T.  Lovell Jonathan C.  Eya 《Journal of the World Aquaculture Society》1998,29(1):31-39

Phosphorus budgets were prepared for channel catfish Ictalurus punctatus ponds at Auburn, Alabama, that received one of five diets ranging from 0.60 to 1.03% phosphorus. Fish production did not differ ( P > 0.05) among diets. There were few differences among treatments with respect to soluble reactive phosphorus, total phosphorus, and chlorophyll a concentrations or gross primary productivity. Phosphorus loss in effluents when ponds were drained for harvest did not differ among treatments ( P > 0.05). Phosphorus removed from ponds in fish at harvest and the amounts of phosphorus adsorbed by bottom soils increased as dietary phosphorus concentration increased (P < 0.05). Low-phosphorus diets did not decrease phytoplankton productivity or improve effluent quality. Uptake of phosphorus by bottom soils is a major factor controlling phosphorus concentrations in pond water. Low-phosphorus diets can be beneficial in catfish pond management by reducing the phosphorus load to bottom soils and conserving their ability to adsorb phosphorus.  相似文献   

Pulmonary silicosis in three North American river otters (Lutra canadensis).     

W K Suedmeyer  G Johnson  M A Lovell 《Journal of zoo and wildlife medicine》1999,30(4):564-572

Three adult female North American river otters (Lutra canadensis) demonstrated severe anesthetic complications shortly after being immobilized for dental procedures. Two of the animals died shortly after anesthesia, and the third otter died 2 mo after immobilization. All three animals were diagnosed with pulmonary silicosis on the basis of histopathology, polarized light microscopy, and mass spectrometry. One animal also had primary pulmonary bronchoalveolar carcinoma. Significant concurrent disease was not found in other organs. Analysis of the lung tissue by laser microbe mass spectrometry confirmed the presence of an aluminum silicate in the lung tissue associated with insulation material used in nest box construction.  相似文献   

Letters to the editor     

Lovell B 《Science (New York, N.Y.)》1966,151(3717):1480-1482

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Myofibroblastic fibrosarcoma with multifocal osseous metaplasia at the site of equine influenza vaccination     

NJ Kannegieter  KL Schaaf  DK Lovell  CD Simon  BM Stone 《Australian veterinary journal》2010,88(4):132-136

We describe a fibrosarcoma in a 12-year-old Quarterhorse × Arabian gelding as a sequela to equine influenza vaccination. Shortly after the second vaccination, swelling at the site was noticed by the owner and it continued to increase in size over the following 6 months. Biopsy of the mass indicated a fibrosarcoma had developed at the vaccination site. It was approximately 20 cm in diameter and elevated well above the level of the skin. There was no clinical evidence of metastases to the lungs or local lymph nodes. Surgical resection of the mass was performed and the wound healed by first and second intention. Histopathological examination and immunohistochemical staining confirmed a myofibroblastic fibrosarcoma with multifocal osseous metaplasia. To the authors' knowledge, this is the first equine case of a vaccine-associated fibrosarcoma.  相似文献   

Nitrogen mineralization and microbial activity in permanent pastures amended with nitrogen fertilizer or dung   总被引：9，自引：0，他引：9  

D. J. Hatch  R. D. Lovell  R. S. Antil  S. C. Jarvis  P. M. Owen 《Biology and Fertility of Soils》2000,30(4):288-293

 Gross rates of soil processes and microbial activity were measured in two grazed permanent pasture soils which had recently been amended with N fertilizer or dung. ¹⁵N studies of rates of soil organic matter turnover showed gross N mineralization was higher, and gross N immobilization was lower, in a long-term fertilized soil than in a soil which had never received fertilizer N. Net mineralization was also found to be higher in the fertilized soil: a consequence of the difference between the opposing N turnover processes of N mineralization and immobilization. In both soils without amendments the soil microbial biomass contents were similar, but biomass activity (specific respiration) was higher in the fertilized soil. Short-term manipulation of fertilizer N input, i.e. adding N to unfertilized soil, or witholding N from previously fertilized soil, for one growing season, did not affect gross mineralization, immobilization or biomass size and activity. Amendments of dung had little effect on gross mineralization, but there was an increase in immobilization in both soils. Total biomass also increased under dung in the unfertilized soil, but specific respiration was reduced, suggesting changes in the composition of the biomass. Dung had a direct effect on the microbial biomass by temporarily increasing available soil C. Prolonged input of fertilizer N increases soil C indirectly as a result of enhanced plant growth, the effect of which may not become evident within one seasonal cycle. Received: 18 December 1998  相似文献   

Nutrition of aquaculture species.   总被引：6，自引：0，他引：6  

R T Lovell 《Journal of animal science》1991,69(10):4193-4200

Dietary requirements for amino acids and fatty acids have been reported for channel catfish (Ictalurus punctatus), salmonids (Oncorhynchus spp.), common carp (Cyprinus carpio), tilapias (Oreochromis spp.), and eel (Anguilla japonicus). Most of the vitamin and mineral requirements are available for channel catfish and salmonids, and some are available for common carp, tilapia, eel, and other finfish and crustaceans. From this available information, cost-effective feeds can be formulated for the major commercial aquaculture species. Major differences in nutrient requirements between fish and mammals or birds are as follows: fish have a lower digestible energy:protein ratio (8 to 10 kcal of DE/g of CP for fish vs 15 to 20 kcal of DE/g of CP for livestock); fish require n-3 fatty acids and land animals require n-6; fish can absorb minerals from the water, which negates the need for some minerals in the diet; and fish have limited ability to synthesize vitamin C and must depend on a dietary source. Areas for further research include 1) refinement of nutrient requirements of the major culture species considering effects of fish size, temperature, and management; 2) nutrient requirements of crustaceans; 3) effects of nutrition on fish health and product quality; and 4) feeding technology.  相似文献   

The Effect of Two Antibiotic Mixtures on Haemophilus somnus, Campylobacter fetus ssp. venerealis, Mycoplasma bovis, and Ureaplasma diversum in Frozen Bovine Semen     

IJR Visser  EA ter Laak  HB Jansen  O Gerard 《Reproduction in domestic animals》1995,30(2):55-59

A two-step semen-extending protocol was compared to a one-step protocol in its efficiency in inhibiting growth of Haemophilus somnus, Campylobacter fetus ssp. venerealis, Mycoplasma bovis , and Ureaplasma diversum in experimentally infected semen. In both protocols, the effect of an antibiotic mixture of 500 μg gentamycin, 100 μg tylosin, 300 μg lincomycin, and 600 μg spectinomycin (GTLS) was compared to a mixture of 500 IU penicillin, 500 IU streptomycin, 150 μg lincomycin, and 300 μg spectinomycin (PSLS). The one-step extending method was as effective as the two-step extending method. Both antibiotic mixtures were equally effective in controlling C. fetus . For H. somnus and U. diversum , the PSLS mixture was more effective than the GTLS mixture. It was striking that both antibiotic mixtures had no effect in decreasing the numbers of M. bovis .  相似文献   

Quality Evaluation of Four Sources of Brine Shrimp Artemia spp.1     

Carl D. Webster  Richard T. Lovell 《Journal of the World Aquaculture Society》1990,21(3):180-185

A study was conducted to compare fatty acid composition, hatching quality, and size of nauplii from four commercial sources of brine shrimp (Artemia spp.) cyse: China (CH), Colombia (COL), Great Salt Lake (GSL), and San Francisco Bay (SFB). The CH brine shrimp had a comparatively high percentage of 20:5(n-3) fatty acid (eicosapentaenoic acid or EPA), an essential fatty acid for most Iarval fishes, which was 10.4% of the total lipids. The COL, GSL, and SFB sources of cysts contained comparatively low percentages of EPA, 2.9, 1.2, and 1.6%. respectively. Hatching quality was determined by hatching cysts in salt (NaCl) water with a specific gravity of 1.02 at 27 C for 42 h. The COL cysts had the fastest hatching rate, with 50% of the cysts hatching in 13.4 h, while CH cysts hatched at the slowest rate, requiring 25.6 h for 50% of the cysts to hatch. Total percentage hatch was not significantly different among the SFB, COL, and GSL sources, with an average hatching percentage of 84.5, while CH cysts had a significantly lower total percentage hatch of 67.5. The SFB source produced the greatest number of nauplii (1.6 ± 10⁵ per g of cysts) with the smallest length (382 μm), while CH produced the smallest number (8.7 ± 10⁴) with the greatest length (500 μm). These results indicate that there is great variation in nutritional quality, hatching quality, and size of nauplii among commercial sources of brine shrimp cysts, and each of these criteria should be considered in selecting brine shrimp in a development of a feeding strategy for larval culture of a particular species.  相似文献