THE POISONOUS NATURE OF THE STINGING HAIRS OF JATROPHA URENS     

Lutz O 《Science (New York, N.Y.)》1914,40(1034):609-610

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Comment on "Global convergence in the temperature sensitivity of respiration at ecosystem level"     

Graf A  Weihermüller L  Huisman JA  Herbst M  Vereecken H 《Science (New York, N.Y.)》2011,331(6022):1265; author reply 1265

Mahecha et al. (Reports, 13 August 2010, p. 838) estimated the temperature sensitivity of ecosystem respiration (Q(10)) and showed that temperature sensitivity and its site-to-site variability are lower than previously reported. We demonstrate that their Q(10) value of 1.4 is an underestimate if interpreted as the averaged sensitivities of all ecosystem components, because fast temperature fluctuations penetrate poorly into the soil.  相似文献   

Thyroid enlargement and its relationship to clinicopathological parameters and T(4) status in suspected hyperthyroid cats     

Boretti FS  Sieber-Ruckstuhl NS  Gerber B  Laluha P  Baumgartner C  Lutz H  Hofmann-Lehmann R  Reusch CE 《Journal of Feline Medicine and Surgery》2009,11(4):286-292

To relate thyroid size to routine blood parameters and T(4) status the ventral neck of 161 cats with clinical signs consistent with hyperthyroidism was examined by two independent observers using a semi-quantitative palpation system. Thyroid gland size of each side was scored from 0 (non-palpable) to a maximum of 6 (>25 mm). In 127 of the 161 cats, at least one thyroid gland was palpable. The palpation score was significantly correlated with the T(4) concentration. The 17 hyperthyroid cats had significantly higher palpation scores than the 110 euthyroid cats. Euthyroid animals with a palpation score >or=3 were significantly older, had higher body weights, lower alkaline phosphatase, alanine aminotransferase, phosphate, and urine specific gravity, but higher lipase and creatinine concentrations than hyperthyroid cats. Our study demonstrates that although no reliable conclusion on the functional status of the thyroid can be drawn based on its size the likelihood of hyperthyroidism increases with increasing size of the gland.  相似文献   

Feline immunodeficiency. ABCD guidelines on prevention and management     

Margaret J. Hosie  Diane Addie  Sándor Belák  Corine Boucraut-Baralon  Herman Egberink  Tadeusz Frymus  Tim Gruffydd-Jones  Katrin Hartmann  Albert Lloret  Hans Lutz  Fulvio Marsilio  Maria Grazia Pennisi  Alan D. Radford  Etienne Thiry  Uwe Truyen  Marian C. Horzinek 《Journal of Feline Medicine and Surgery》2009,11(7):575-584

OverviewFeline immunodeficiency virus (FIV) is a retrovirus closely related to human immunodeficiency virus. Most felids are susceptible to FIV, but humans are not. Feline immunodeficiency virus is endemic in domestic cat populations worldwide. The virus loses infectivity quickly outside the host and is susceptible to all disinfectants.InfectionFeline immunodeficiency virus is transmitted via bites. The risk of transmission is low in households with socially well-adapted cats. Transmission from mother to kittens may occur, especially if the queen is undergoing an acute infection. Cats with FIV are persistently infected in spite of their ability to mount antibody and cell-mediated immune responses.Disease signsInfected cats generally remain free of clinical signs for several years, and some cats never develop disease, depending on the infecting isolate. Most clinical signs are the consequence of immunodeficiency and secondary infection. Typical manifestations are chronic gingivostomatitis, chronic rhinitis, lymphadenopathy, weight loss and immune-mediated glomerulonephritis.DiagnosisPositive in-practice ELISA results obtained in a low-prevalence or low-risk population should always be confirmed by a laboratory. Western blot is the ‘gold standard’ laboratory test for FIV serology. PCR-based assays vary in performance.Disease managementCats should never be euthanased solely on the basis of an FIV-positive test result. Cats infected with FIV may live as long as uninfected cats, with appropriate management. Asymptomatic FIV-infected cats should be neutered to avoid fighting and virus transmission. Infected cats should receive regular veterinary health checks. They can be housed in the same ward as other patients, but should be kept in individual cages.Vaccination recommendationsAt present, there is no FIV vaccine commercially available in Europe. Potential benefits and risks of vaccinating FIV-infected cats should be assessed on an individual cat basis. Needles and surgical instruments used on FIV-positive cats may transmit the virus to other cats, so strict hygiene is essential.  相似文献   

Chlamydophila felis infection. ABCD guidelines on prevention and management     

Tim Gruffydd-Jones  Diane Addie  Sándor Belák  Corine Boucraut-Baralon  Herman Egberink  Tadeusz Frymus  Katrin Hartmann  Margaret J. Hosie  Albert Lloret  Hans Lutz  Fulvio Marsilio  Maria Grazia Pennisi  Alan D. Radford  Etienne Thiry  Uwe Truyen  Marian C. Horzinek 《Journal of Feline Medicine and Surgery》2009,11(7):605-609

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H5N1 avian influenza in cats. ABCD guidelines on prevention and management     

Etienne Thiry  Diane Addie  Sándor Belák  Corine Boucraut-Baralon  Herman Egberink  Tadeusz Frymus  Tim Gruffydd-Jones  Katrin Hartmann  Margaret J. Hosie  Albert Lloret  Hans Lutz  Fulvio Marsilio  Maria Grazia Pennisi  Alan D. Radford  Uwe Truyen  Marian C. Horzinek 《Journal of Feline Medicine and Surgery》2009,11(7):615-618

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Assessment of six different collagenase-based methods to isolate feline pancreatic islets     

Eric Zini  Marco Franchini  Franco Guscetti  Melania Osto  Karin Kaufmann  Mathias Ackermann  Thomas A. Lutz  Claudia E. Reusch 《Research in veterinary science》2009,87(3):367-372

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Predation on native fish eggs by invasive round goby revealed by species‐specific gut content DNA analyses     

Elisabeth Lutz  Philipp Emanuel Hirsch  Karen Bussmann  Joschka Wiegleb  Hans‐Peter Jermann  Roxane Muller  Patricia Burkhardt‐Holm  Irene Adrian‐Kalchhauser 《水产资源保护:海洋与淡水生态系统》2020,30(8):1566-1577

1. Conservation of riverine fish often aims to improve access to spawning grounds and restore longitudinal connectivity by removing migration barriers, and involves substantial investments. However, these investments also enable non‐native predators to invade upstream into spawning areas and potentially adversely affect the recruitment of threatened freshwater fish through egg or fry predation.
2. Detecting egg predation is often challenging. Visual inspections of fish gut contents may underestimate predation of soft materials such as eggs and fry, which limits the discovery of predators preying upon these life‐stages. DNA‐based detection assays may offer a more sensitive tool to assess predation of soft materials.
3. A conservation issue was confirmed by developing and applying a species‐specific DNA‐based detection assay: invasive round goby (Neogobius melanostomus) prey on the eggs or fry of the threatened common nase (Chondrostoma nasus) in Switzerland.
4. DNA‐based detection assays were also developed for five other valuable native fish species, including endangered salmonid and cyprinid river spawners. The applicability of the assays was confirmed in a series of laboratory and field feeding experiments involving eggs and fish tissue. In addition, this work provides a guiding framework for conservation managers regarding the use and applicability of different DNA‐based detection approaches for gut content analysis.
5. The results of this study could inform local conservation measures – such as temporary reductions in the density of round goby at spawning sites prior to spawning – and demonstrate how targeted application of species‐specific molecular markers may advance freshwater fish management.

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Identification of disulfide bonds in wheat gluten proteins by means of mass spectrometry/electron transfer dissociation     

Lutz E  Wieser H  Koehler P 《Journal of agricultural and food chemistry》2012,60(14):3708-3716

Disulfide bonds within gluten proteins play a key role in the breadmaking performance of wheat flour. In the present study, disulfide bonds of wheat gluten proteins were identified by using a new liquid chromatography-mass spectrometry (LC-MS) technique with alternating electron transfer dissociation (ETD)/collision-induced dissociation (CID). Wheat flour was partially hydrolyzed with thermolysin (pH 6.5, 37 °C, 16 h), and the digest was subjected to LC-MS with alternating ETD/CID fragmentation. Whereas CID provided peptide fragments with intact disulfide bonds, cleavage of disulfide bonds was preferred over peptide backbone fragmentations in ETD. The simultaneous observation of disulfide-linked and disulfide-cleaved peptide ions in the mass spectra not only provided distinct interpretation with high confidence but also simplified the conventional approach for determination of disulfide bonds, which often requires two separate experiments with and without chemical reduction. By application of the new method 14 cystine peptides were identified. Eight peptides confirmed previously established disulfide bonds within gluten proteins, and the other six cystine peptides were identified for the first time. One of the newly identified cystine peptides represented a "head-to-tail" cross-link between high molecular weight glutenin subunits. This type of cross-link, which has been postulated as an integral part of glutenin models published previously, has now been proven experimentally for the first time. From the six remaining cystine peptides interchain disulfide bonds between α-gliadins, γ-gliadins, and low molecular weight glutenin subunits were established.  相似文献   

Mechanische Fruchtbehangsregulierung bei Apfel verbessert Fruchtgröße,Fruchtfestigkeit, Fruchtausfärbung und die Source: Sink – Verhältnisse mit mehr Einzelfruchtständen (Singlets) bei ‘Gala’     

Claudia Seehuber  Lutz Damerow  Achim Kunz  Michael M. Blanke 《Erwerbs-Obstbau》2014,56(2):49-58

The objective of the MSc project was to improve fruit quality, i.e. fruit firmness, colour and size, by mechanical blossom thinning. Ten-year-old slender spindle apple cv. ‘Gala, Mondial’ trees at Campus Klein-Altendorf near Bonn, Germany with an intense flowering of 7–8 on the 0–9 scale were blossom-thinned on 21 April 2009; unthinned trees served as control. Rotor speeds were 360 rpm or 420 rpm at 5 or 7.5 km/h tractor speed using the new mechanical thinning device developed at the University of Bonn in 2004–2006. Therein, a vertical mast supports three horizontal rotors, whose vertically rotating tines remove excessive flowers; the device was mounted on the front three point hitch of the tractor with the following results:

1. Mechanical thinning reduced the number of apple fruit from 18 per branch section in the un-thinned control, 12–13 in the medium to 8 fruit in the strongest thinning (420 rpm, 5 km/h).
2. Similarly, the number of singlets (one fruitlet/flower cluster) nearly doubled from ca. 2 per branch in the control to 3–4 after mechanical thinning, as intended.
3. The natural June drop after mechanical thinning resembled that in the un-thinned control.
4. Mechanical thinning increased fruit mass by 20 g—48 g relative to the un-thinned control.
5. Mechanical thinning with 420 rpm increased the portion of premium fruit >?70 mm (class I) by 43?% (7.5 km/h) and 63?% (5 km/h); combined mechanical and hand-thinning yielded the largest portion of fruit >?70 mm (70?%), but with a dramatic loss in yield (55?%).
6. Mechanical and manual thinning improved the portion of well coloured fruit (75–100?% coloured) (78–98?%) when compared to the un-thinned control fruits with 55?%, probably due to selective removal of shaded fruits from the inner tree canopy.
7. Overall, mechanical thinning alone gave better results than hand thinning alone or its combination with hand-thinning in cv. ‘Gala’; mechanical thinning waived the need for hand thinning in this experiment.
8. Mechanical thinning improved fruit firmness from 9.4 kg/cm² in the un-thinned control to 10.4 kg/cm².
9. Neither vegetative growth nor subsequent fruit drop were stimulated by this type of mechanical thinning.
10. The risk of both alternate bearing and over-sized fruit was waived due to the variety employed.
11. An economic evaluation showed a net profit of € 220/ha in the weak (420 rpm—7.5 km/h) aνô € 1,844/ha in the intermediate (360 rpm—5 km/h), but a net loss of € 1,120/ha (420 rpm—5 km/h) for the strongest thinning treatment, assuming a cost of € 120/ha for the mechanical treatment and a saving of € 200 for reduced hand thinning.

Overall, the positive results of a better fruit quality (size, colour and firmness) offer the potential of mechanical blossom thinning in cv. ‘Gala’, which is difficult to blossom-thin chemically.  相似文献