Effect of constant infusion of oxytocin on luteal lifespan and oxytocin-induced release of prostaglandin F₂α in heifers     

S.L. Lutz  M.F. Smith  D.H. Keisler  H.A. Garverick 《Domestic animal endocrinology》1991,8(4):573-585

Two experiments were conducted to determine whether constant infusion of oxytocin would prolong the luteal phase and inhibit uterine prostaglandin F2 alpha (PGF2 alpha) secretion in heifers. In Experiment 1, twelve heifers, treated with saline (SAL) or oxytocin (OXY) via jugular cannulae infusions (INF) or osmotic minipumps (OMP), were allotted at estrus into four treatment groups (n = 3). Treatments were: SAL-INF, SAL-OMP, OXY-INF and OXY-OMP. Physiological saline or oxytocin was given from Days 10 to 23 (Day 0 = estrus) of the estrous cycle. Method of treatment (jugular cannula infusion or osmotic minipump) had no effect (P greater than 0.05) on estrous cycle length or pattern of secretion of progesterone; therefore, data were pooled. Estrous cycle lengths were extended (P less than 0.01) for heifers which received oxytocin (25.3 +/- 0.4 d) compared to saline (20.5 +/- 0.4 d). Luteolysis did not occur in oxytocin-treated heifers until after treatment ceased. Experiment 2 was designed and conducted identically to Experiment 1 with the addition of a "challenge" injection of oxytocin (100 IU oxytocin, i.v.) given on Day 16 of the estrous cycle. Treatment of heifers with oxytocin extended (P less than 0.05) estrous cycle length by an average of 3 d compared to heifers treated with saline. The "challenge" injection induced (P less than 0.05) secretion of PGF2 alpha (as measured by the stable PGF2 alpha metabolite, 15-keto-13,14-dihydro-PGF2 alpha) in saline-treated but not oxytocin-treated heifers. In both Experiment 1 and 2, serum concentrations of FSH were elevated (P less than 0.05) in oxytocin-treated heifers. No increase was observed for LH or prolactin. The rise in estradiol-17 beta at luteolysis was not affected (P greater than 0.10) by treatment. In summary, constant infusion of oxytocin extended luteal lifespan, prolonged secretion of progesterone, and inhibited oxytocin-induced secretion of PGF2 alpha. Constant infusion of oxytocin did not affect serum concentrations of estradiol-17 beta, LH or prolactin; however, serum concentrations of FSH were elevated during the oxytocin treatment period.  相似文献   

Haematology, blood chemistry and urine parameters of free-ranging plains viscachas (Lagostomus maximus) in Argentina determined by use of a portable blood analyser (i-STAT) and conventional laboratory methods     

Wenker CJ  Hunziker D  Lopez J  Oppliger H  Forrer R  Lutz H 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2007,54(5):260-264

Haematology, blood chemistry and urine values were determined for 44 adult free-ranging plains viscachas (Lagostomus maximus; Rodentia, Chinchillidae) in their pampas habitat in central Argentina. The study animals were captured in the wild and anaesthetized with a ketamine-xylazine combination for physical examination and sampling. Blood was obtained by venipuncture of the saphenous vein. Results for many of the blood parameters fall within the reference ranges for pet chinchillas. Differentiation of white blood cells revealed a predominantly neutrophil count for plains viscachas, while chinchillas have predominantly lymphocytes. Mean values for blood urea nitrogen, creatinine, aspartate aminotransferase and sodium were higher than the upper limit of the reference range for pet chinchillas. The results of seven analytes (haematocrit, haemoglobin, glucose, blood urea nitrogen, sodium, potassium, chloride) were compared by using both a portable blood analyser (i-STAT) in the field and conventional laboratory methods. In general, correlation and agreement between the two methods were low for most parameters.  相似文献   

Transition from maternal to endogenous antibodies in newborn calves     

Hassig M  Stadler T  Lutz H 《The Veterinary record》2007,160(7):234-235

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Prevalence of Fasciola hepatica in the intermediate host Lymnaea truncatula detected by real time TaqMan PCR in populations from 70 Swiss farms with cattle husbandry     

Schweizer G  Meli ML  Torgerson PR  Lutz H  Deplazes P  Braun U 《Veterinary parasitology》2007,150(1-2):164-169

Bovine fasciolosis is an economically important parasitic disease. Quantitative real time PCR was utilized to determine the prevalence of Fasciola hepatica in the snail intermediate host Lymnaea truncatula from 70 selected, infected Swiss cattle farms, and to gain information on the infection risk to the definitive host. Snails from 130 habitats (36 streams, 21 wells, 24 drainage ditches, 33 spring swamps, 14 reeds, 1 drainage shaft and 1 pond) originating from 71 dairy cow pastures, 39 pastures for young stock, 14 hay fields and 6 dry cow pastures were collected. Of these, 51 populations were found to be infected with F. hepatica. A total of 4733 snails were examined of which 331 were infected (7.0%). The numbers of snails collected from different sites ranged from 1 to 159 snails. Clustering of infection in snails was found on the farm of origin with a mixed logistic model with random effects. The risk of infection of L. truncatula with F. hepatica was significantly higher in populations originating from spring swamps, wells and reeds compared to populations from streams. In addition the risk of snail infection was significantly lower in populations collected in young stock and dry cow pastures compared to dairy cow pastures. The greater the population size collected from a habitat also increased the risk of an individual snail being infected.  相似文献   

Highly pathogenic avian influenza H5N1 virus in cats and other carnivores     

Thiry E  Zicola A  Addie D  Egberink H  Hartmann K  Lutz H  Poulet H  Horzinek MC 《Veterinary microbiology》2007,122(1-2):25-31

The Asian lineage highly pathogenic avian influenza (HPAI) H5N1 virus is a known pathogen of birds. Only recently, the virus has been reported to cause sporadic fatal disease in carnivores, and its zoonotic potential has been dominating the popular media. Attention to felids was drawn by two outbreaks with high mortality in tigers, leopards and other exotic felids in Thailand. Subsequently, domestic cats were found naturally infected and experimentally susceptible to H5N1 virus. A high susceptibility of the dog to H3N8 equine influenza A virus had been reported earlier, and recently also HPAI H5N1 virus has been identified as a canine pathogen. The ferret, hamster and mouse are suitable as experimental animals; importantly, these species are also kept as pets. Experimental intratracheal and oral infection of cats with an HPAI H5N1 virus isolate from a human case resulted in lethal disease; furthermore, cats have been infected by the feeding of infected chickens. Spread of the infection from experimentally infected to in-contact cats has been reported. The epidemiological role of the cat and other pet animal species in transmitting HPAI H5N1 virus to humans needs continuous consideration and attention.  相似文献   

Efficacy of a modified live intranasal bovine respiratory syncytial virus vaccine in 3-week-old calves experimentally challenged with BRSV     

Vangeel I  Antonis AF  Fluess M  Riegler L  Peters AR  Harmeyer SS 《Veterinary journal (London, England : 1997)》2007,174(3):627-635

Two experimental bovine respiratory syncytial virus (BRSV) challenge studies were undertaken to evaluate the efficacy of a single intranasal dose of a bivalent modified live vaccine containing BRSV in 3-week-old calves. In the first study, vaccine efficacy was evaluated in colostrum deprived (maternal antibody negative) calves 5, 10 and 21 days after vaccination. Nasal shedding of BRSV was significantly reduced in vaccinated calves challenged 10 or 21 days after vaccination. Virus excretion titres were also reduced in vaccinates challenged 5 days after vaccination but reduction in duration of shedding and total amount of virus shed were not statistically significant. Clinical disease after challenge in this study was mild. In the second study, vaccine efficacy was assessed in calves with maternal antibodies against BRSV by challenge 66 days post-vaccination. Vaccination significantly reduced nasal shedding after challenge and the severity of clinical disease was also reduced.  相似文献   

Longitudinal Evaluation of Serum Pancreatic Enzymes and Ultrasonographic Findings in Diabetic Cats Without Clinically Relevant Pancreatitis at Diagnosis          下载免费PDF全文

E. Zini  M. Hafner  P. Kook  T.A. Lutz  S. Ohlerth  C.E. Reusch 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2015,29(2):589-596

Background

Cats with diabetes mellitus can have subclinical pancreatitis but prospective studies to confirm this are lacking. Metabolic control of diabetic cats with pancreatitis is difficult.

Hypothesis

Subclinical pancreatitis occurs in diabetic cats at the time diabetes is diagnosed or might develop during the follow‐up period, hampering diabetic remission.

Animals

Thirty cats with newly diagnosed diabetes without clinical signs of pancreatitis on admission.

Methods

Prospective study. On admission and 2 and 6 months later, serum Spec fPL and DGGR‐lipase were measured and the pancreas underwent ultrasonographic examination. Pancreatitis was suspected if serum markers were increased or ≥2 ultrasonographic abnormalities were detected. Cats were treated with insulin glargine and diabetic remission was defined as euglycemia ≥4 weeks after discontinuation of insulin. Nonparametric statistical tests were used for analysis.

Results

Subclinical pancreatitis at the time of diagnosis was suspected in 33, 50, and 31% of cats based on Spec fPL, DGGR‐lipase and ultrasonography, respectively; and in 60% when diagnostic criteria were combined. During the follow‐up period, suspected pancreatitis developed in additional 17–30% cats. Only 1 cat had transient clinical signs compatible with pancreatitis. Seventeen of the 30 cats (57%) achieved remission. Frequency of abnormal Spec fPL and DGGR‐lipase and abnormal ultrasonographic findings did not differ in cats achieving remission and those who did not. Cats achieving remission had significantly lower Spec fPL at 2 months (P < .001).

Conclusions and Clinical Importance

Based on laboratory and ultrasonographic measurements, many cats with diabetes might have pancreatitis, although without clinical signs. Cats with high Spec fPL might have a reduced chance of diabetic remission; however, this topic needs further studies in large cohorts of diabetic cats.  相似文献   

Oestrogen and Progesterone Receptors and COX‐2 Expression in Endometrial Biopsy Samples During Maternal Recognition of Pregnancy in Llamas (Lama glama)          下载免费PDF全文

CP Bianchi  A Meikle  MA Benavente  MA Álvarez  VL Trasorras  MH Miragaya  E Rodríguez  MA Aba 《Reproduction in domestic animals》2015,50(6):980-988

Endometrial expression of oestrogen receptor‐α (ERα), progesterone receptor (PR) and cyclooxigenase‐2 (COX‐2) was evaluated in non‐pregnant and pregnant llamas during the period when luteolysis/maternal recognition of pregnancy is expected to occur. Females (n = 28) were divided into two groups: non‐pregnant llamas were induced to ovulate with a Buserelin injection, and endometrial biopsies were obtained on day 8 (n = 5) or 12 (n = 5) post‐induction of ovulation. Animals of the pregnant group (n = 18) were mated with a fertile male. Pregnancy was confirmed by the visualization of the embryo collected by transcervical flushing in 5 of 9 animals on day 8 post‐mating and by progesterone profile on day 12 post‐mating in 4 of 9 animals, when endometrial biopsies were obtained. An immunohistochemical technique was used to evaluate receptors population and COX‐2 expression. Pregnant llamas showed a higher percentage of positive cells and stronger intensity for ERα than for non‐pregnant llamas in stroma on day 8 and in the luminal epithelium on day 12 post‐induction of ovulation, while a deep decrease in endometrial PR population was reported in pregnant llamas on that day in luminal and glandular epithelia and stroma. In the luminal epithelium, COX‐2 expression was lower in pregnant than in non‐pregnant animals. Briefly, the increase of ERα in pregnant llamas gives further support to the hypothesis that oestrogens are involved in the mechanism of maternal recognition of pregnancy. Endometrial PR decrease in pregnant llamas might be a necessary event to allow the expression of proteins involved in conceptus attachment, a mechanism widely accepted in other species. Moreover, embryo seems to attenuate maternal PGF_2α secretion during early pregnancy by decreasing the endometrial expression of COX‐2 in the luminal epithelium of pregnant llamas.  相似文献   

Mechanische Fruchtbehangsregulierung verringerte den Junifruchtfall und verbesserte die Fruchtqualität und Fruchtgröße bei ‚Conference‘- und ‚Alexander Lucas‘-Birnen     

Dipl.-Ing. Claudia Seehuber  Dr. Lutz Damerow  Achim Kunz  Dr. Michael Blanke 《Erwerbs-Obstbau》2010,52(2):45-53

In the absence of any chemicals approved for thinning pears but market demand for large particularly cv. ‘Conference’ fruit, the objective of the present study as part of a MSC thesis was to improve fruit quality i.e. achieve these fruit sizes by mechanical blossom thinning. Therefore, 18-year-old cv. ‘Conference’ pear trees, trained as super spindles, on quince A rootstock with a spacing of 4?×?0.4 m were used in Klein-Altendorf near Bonn, Germany; cv. ‘A. Lucas’ and un-thinned trees served as control. These pear trees with intense flowering were blossom-thinned on 15 April 2009 with rotor speeds of either 300 rpm or 400 rpm both at 5 km/h tractor speed with the new mechanical thinning device developed at the University of Bonn in 2004–2006. Therein, a vertical mast supports three horizontal rotors, whose vertically rotating tines remove excessive flowers; the device is mounted on the front three point hitch of the tractor. In 2009, a year with severe June drop, mechanical thinning reduced the intensity of the natural June drop with increasing rotor speed and was stronger reduced in cv. ‘A. Lucas’ (by ?38%) than in cv. ‘Conference’ (by ?27% relative to the control). In cv. ‘A. Lucas’, the natural June drop was as strong as mechanical thinning and resulted in large-sized 80 mm pear fruit in the control; mechanical thinning with 400 rpm further increased fruit mass by 16 g relative to the un-thinned control or 6 g larger than in the thinning with 300 rpm. In cv. ‘Conference’, fruit fell within the next larger fruit size class after the stronger mechanical thinning with 400 rpm at 5 km/h: the portion of pear fruit in the 75/80 mm size class was 2 fold and in the 80/85 mm size class was 6 fold that in the control. The portion of <?60, 60/65 mm and 65/70 mm fruit was halved relative to the control; the average fruit mass was increased by 40 g. Vegetative growth appeared unaffected by mechanical thinning. The risk of both over-sized fruit and over-thinning appears small, since mechanical thinning reduced the natural June drop and did not induce subsequent fruit drop. Mechanical thinning was more efficient in cv. ‘Conference’, as intended, despite more intense flowering in cv. ‘A Lucas’. An economic evaluation proved difficult, since the natural June drop particularly in cv. ‘A. Lucas’ led to large-sized fruit (80 mm) in the control, and any (mechanical) thinning is associated with lower yields (?26% in cv. ‘Conference’ and ?12% in cv. ‘A. Lucas’). Overall, the results indicate the potential of mechanical thinning as a substitute (or combination partner for chemical thinning if approved) in pear.  相似文献   

Evaluation of a new in‐clinic test system to detect feline immunodeficiency virus and feline leukemia virus infection     

Christina Sand  Theresa Englert  Herman Egberink  Hans Lutz  Katrin Hartmann 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2010,39(2):210-214

Background: Many in‐house tests for the diagnosis of feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) infection are licensed for use in veterinary practice. A new test with unknown performance has recently appeared on the market. Objectives: The aims of this study were to define the efficacy of a new in‐clinic test system, the Anigen Rapid FIV Ab/FeLV Ag Test, and to compare it with the current leading in‐clinic test, the SNAP Kombi Plus FeLV Antigen/FIB Antibody Test. Methods: Three‐hundred serum samples from randomly selected healthy and diseased cats presented to the Clinic of Small Animal Medicine at Ludwig Maximilian University were tested using both the Anigen Rapid Test and the SNAP Kombi Plus Test. Diagnostic sensitivity, specificity, and positive and negative predictive values were calculated for both tests using Western blot as the gold standard for verification of FIV infection and PCR as the gold standard for FeLV infection. Results: The presence of antibodies against FIV was confirmed by Western blot in 9/300 samples (prevalence 3%). FeLV DNA was detected by PCR in 15/300 samples (prevalence 5%). For FIV infection the Anigen Rapid Test had a sensitivity of 88.9%, specificity of 99.7%, positive predictive value of 88.9%, and negative predictive value of 99.7%. For FeLV infection, the Anigen Rapid Test had a sensitivity of 40.0%, specificity of 100%, positive predictive value of 100%, and negative predictive value of 96.9%. Diagnostic accuracy was similar to that of the SNAP Kombi Plus Test. Conclusion: The new Anigen Rapid FIV Ab/FeLV Ag Test performed very well and can be recommended for use in veterinary practice.  相似文献