Monoclonal antibodies to bovine viral diarrhea virus: cross-reactivities to field isolates and hog cholera virus strains.   总被引：2，自引：1，他引：1       下载免费PDF全文

D Deregt  S R Bolin  R A Heckert    K G Loewen 《Canadian journal of veterinary research》1994,58(1):71-74

Monoclonal antibodies to bovine viral diarrhea virus (BVDV) were examined for binding with a large number of North American BVDV isolates and eight strains of the serologically related pestivirus, hog cholera virus (HCV). No single BVDV monoclonal antibody reacted with all BVDV isolates. The most cross-reactive monoclonal antibody was an anti-p80/p125 antibody which showed a positive reaction with 173 of 180 (96%) North American isolates. From a fewer number of isolates tested, one anti-gp53 monoclonal antibody also showed a high cross-reactivity (94%). All BVDV isolates showed a positive reaction with at least one of the seven monoclonal antibodies in the panel. Thus, the results indicated that a pool of these monoclonal antibodies may be used in place of polyclonal antisera for the detection of BVDV contamination of cell lines or for virus isolation. For HCV, all three anti-p80/p125 monoclonal antibodies reacted positively with all eight virus strains. In contrast, none of the anti-gp53 monoclonal antibodies were reactive to HCV strains. Thus, the anti-gp53 monoclonal antibodies may be useful for distinguishing between usually innocuous BVDV infections and the highly significant HCV infections in swine for foreign animal disease surveillance.  相似文献   

Comparative effects of mu and kappa opiate agonists on the cecocolic motility in the pony.          下载免费PDF全文

T Roger  T Bardon    Y Ruckebusch 《Canadian journal of veterinary research》1994,58(3):163-166

The electrical and mechanical activity of the large intestine and its response to administration of opiate mu and kappa agonists were assessed from electrodes and inductograph coils chronically implanted on the cecocolic segment in six ponies given a diet of hay and concentrates. Before the drugs were given, migrating complexes propagating from the cecum into the colon occurred at the rate of 1.5 to 16/hour. During this propulsive activity, the cecocolic sphincter opened and closed allowing the outflow of cecal contents and preventing the backflow of colic contents. Each pony was used as its own control and was given fentanyl (0.01 and 0.05 mg/kg of body weight, IV) and U50488H (0.1 and 0.5 mg/kg, IV) at weekly intervals. The mu agonist fentanyl elicited a marked phase of inhibition of the propulsive activity and a closure of the cecocolic sphincter that lasted one to two hours depending on the dose. The kappa agonist U50488H induced an inhibition of the short spiking activity, i.e. of the resting muscle tone. It did not disturb the occurrence of migrating complexes nor that of the openings of the cecocolic sphincter. These kappa compounds may be drugs of choice to alleviate visceral pain in colic stases without inducing delay of transit unlike mu compounds.  相似文献   

灌区节水改造对塔里木河流域治理的作用   总被引：3，自引：0，他引：3  

李亚娟  杨晴  关春曼 《中国农村水利水电》2001,1(6):4-7

在对塔里木河“四源一干”及流域大型灌区存在主要问题分析的基础上，探讨了大型灌区建戌节水改造,对工业及城镇生活需水增长、灌溉及周边生态环境用水、流域综合治理和社会经济可持续发展的作用，深入阐明大型灌区续建配套与节水改造对区域水资源合理配置生态及环境发展的重大意义。  相似文献   

冷季型草坪草建植管理技术研究     

邓蓉  向清华  梁应林 《草业科学》2001,18(6):70-74

采用正交设计进行施肥里、刈割高度、播量，草坪草品种4因素4水平试验，通过测定草坪草越冬率，越夏率，成坪时间，叶绿素，杂草盖度，生长度，初步筛选出不同目的冷季型草坪草的建植管理方案。  相似文献   

葡萄品种需冷量及打破休眠研究   总被引：23，自引：3，他引：20  

杨天仪  李世诚  蒋爱丽  骆军  金佩芳  单传伦 《果树学报》2001,18(6):321-324

对上海地区葡萄促成栽培的几个主要品种的休眠需冷量进行了调查,发现在供试的5个品种中以里扎马特的需冷量最低,自然条件下仅为447.5h;其次为京亚和巨峰,需冷量均低于1000h;绯红和无核白鸡心需冷量最高,均超过1000h。由此推算,上海地区葡萄结束自然休眠的时间在1月上旬至2月中旬。打破休眠的试验表明以20%石灰氮12月中旬处理效果最佳。  相似文献   

葡萄二倍体与四倍体品种间杂交胚珠的离体培养   总被引：12，自引：2，他引：10  

徐海英  阎爱玲  张国军 《果树学报》2001,18(6):317-320

为了克服葡萄二倍体品种与四倍体品种之间的交配障碍,进行了杂交胚珠的离体培养研究。发芽胚珠的取样时期集中于授粉后35～75d,其中多数以二倍体作母本的组合在授粉后55d取样的发芽率最高,以四倍体作母本的组合授粉后70d取样的发芽率最高。基本培养模式为:发育培养基-(剥胚)萌发培养基-生长培养基。用二倍体品种作母本时,所有组合均获得了培养苗,在1号培养基上培养的胚珠发芽率高于2号培养基。反交对培养基的要求有相反趋势,其中2个组合未得到培养苗。授粉后60d,直接剥出杂种胚进行培养,得到了最高发芽率。综合分析初步认为,用二倍体品种作母本比用四倍体品种作母本容易获得杂交后代。  相似文献   

Pharmacokinetic evaluation of ceftiofur in serum, tissue chamber fluid and bronchial secretions from healthy beef-bred calves.          下载免费PDF全文

S L Halstead  R D Walker  J C Baker  R E Holland  G E Stein    J G Hauptman 《Canadian journal of veterinary research》1992,56(4):269-274

Ceftiofur is a new broad spectrum cephalosporin marketed for the treatment of acute bovine respiratory disease. In this investigation ceftiofur was administered by intramuscular injection, at 24 h intervals, to healthy beef-bred calves for four days at dosages of 2.2 and 4.4 mg/kg of body weight, with 4 wk intervals between dosing regimens. Serum, tissue chamber fluid (TCF), and bronchial secretion (BS) concentrations of ceftiofur were measured by microbiological assay after the first and fourth dose of each dosing regimen. Peak serum concentrations (Cmax) of 8.8 micrograms/mL and 17.3 micrograms/mL were obtained approximately 2 h (Tmax), the time of mean peak concentration) after single injections of 2.2 mg/kg and 4.4 mg/kg, respectively. The Cmax was increased approximately twofold following multiple doses of 2.2 mg/kg (Cmax = 13.1 micrograms/mL) and 4.4 mg/kg (Cmax = 24.1 micrograms/mL). Ceftiofur accumulated slowly into TCF and peak concentrations were found to be approximately 14% of those observed in serum after the first dose and approximately 24% after multiple dosing. Concentrations of ceftiofur in BS were obtained rapidly with peak concentrations reaching 45% of the serum Cmax after the first dose. After multiple dosing the Cmax for BS was approximately 25% of the serum Cmax. This study found that both the 2.2 mg/kg and 4.4 mg/kg dosing regimens resulted in continuous serum, TCF and BS concentrations of ceftiofur that exceeded the minimal concentration required to inhibit the bacteria most frequently isolated from calves with acute bovine respiratory disease.  相似文献   

越桔对长白山区酸性土壤的适应性   总被引：15，自引：1，他引：14  

李亚东  郝瑞 《园艺学报》1994,21(2):129-133

在长白山区３种类型的酸性土壤上栽培越桔，研究结果表明，｀北空＇、｀美登＇、｀斯卫克＇在暗棕色森林土上生长结果较好，树体营养关系合理；在草甸沼泽土上３个种生长结果均较差，｀北空＇在水湿地潜育土上生长旺盛，但产量低，综合分析认为，矮丛越桔类型品种对我国长白山区酸性土壤条件的适应性较强，半高丛越桔则适应性差，针对长白山不同类型的土壤，以美登最为适宜暗棕色森林土栽培，斯卫克次之；水湿地潜育土可栽培美登品种  相似文献   

野大麦人工栽培驯化试验   总被引：1，自引：0，他引：1  

董卫民  李阜憬 《草业科学》1992,9(5):38-39

  相似文献   

湖南省果梅品种资源种质杂化状况的初步研究   总被引：8，自引：0，他引：8  

刘连森  贺善文 《园艺学报》1993,20(3):225-230

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