Radiographic Abnormalities in Barrel Racing Horses with Lameness Referable to the Metacarpophalangeal Joint     

Bruno Carvalho Menarim  Vânia Maria Vasconcelos Machado  Luís Emiliano Cisneros Alvarez  Rodrigo Carneiro  Leandro Busch  Luiz Carlos Vulcano 《Journal of Equine Veterinary Science》2012

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Different levels of organic trace minerals in diets for Nile tilapia juveniles alter gut characteristics and body composition,but not growth     

Bruno da Silva Pierri  Allan David Silva  Douglas Ismael Cadorin  Tamiris Henrique Ferreira  Jos Luiz Pedreira Mourio  Keith Filer  James E. Pettigrew  Dbora Machado Fracalossi 《Aquaculture Nutrition》2021,27(1):176-186

Thirty groups of twenty juveniles each of Nile tilapia (Oreochromis niloticus, initial average weight 23.93 ± 0.80 g) were fed diets containing increasing amounts (0%, 25%, 50%, 75%, and 100% of levels used by the tilapia feed industry) of organic trace mineral (Fe, Zn, Mn, Cu, Se) supplementation for 56 days. Growth was not affected by the dose of mineral supplementation. However, body protein increased in fish fed intermediate doses of organic minerals, reaching a maximum at 40.44% supplementation. Doses >50% showed mild damage to the intestinal tissue. Body Fe, Zn and Cu deposition were highest at 28.32%, 24.43% and 78.45% supplementation, respectively. Tilapia could modulate intestinal morphology when fed different doses of organic minerals. As the supplementation doses increased, hemosiderin staining increased in the distal intestine and total body Fe content, indicating that the distal region of the intestine plays an important role in iron excretion by tilapia. Supplementation of organic trace minerals at intermediate doses does not harm Nile tilapia growth, but excessive trace mineral supplementation can impair body protein retention and promote histological alterations in the gut.  相似文献   

Removal of Chromium, Copper, and Nickel from an Electroplating Effluent Using a Flocculent Brewer’s Yeast Strain of Saccharomyces cerevisiae   总被引：1，自引：0，他引：1  

Manuela D. Machado  Helena M. V. M. Soares  Eduardo V. Soares 《Water, air, and soil pollution》2010,212(1-4):199-204

The release of heavy metals in aquatic systems due to the discharge of industrial wastewaters is a matter of environmental concern. Heat-inactivated cells of a flocculent strain of Saccharomyces cerevisiae were used in the bioremediation, in a batch mode, of a real electroplating effluent containing Cu, Ni, and Cr. In this approach, no previous reduction of Cr(VI) to Cr(III) was required. Cr(VI) was selectively removed (98%) by yeast biomass at pH 2.3. At this pH, Cr(VI) is mainly in the form of HCrO ₄ ^? and yeast surface is surrounded by H⁺ ions, which enhance the Cr(VI) interaction with biomass binding sites by electrostatic forces. Subsequently, pH of the effluent was raised up to 6.0; this pH maximizes the efficiency of cations removal since at this pH the main binding groups of yeast cells are totally or partially deprotonated. The passage of effluent through a series of sequential batches, at pH 6.0, allowed, after the third batch, the removal of Cu(II), Ni (II), Cr total, and Cr(VI) in the effluent to values below the legal limit of discharge. The strategy proposed in the present work can be used in plants for the treatment of heavy metals rich industrial effluents containing simultaneously Cr(VI) and Cr(III).  相似文献   

Morphological and molecular characterization of Colletotrichum spp. from citrus orchards affected by postbloom fruit drop in Brazil     

E.E. Kuramae-Izioka  C.R. Lopes  N.L. Souza  M.A. Machado 《European journal of plant pathology / European Foundation for Plant Pathology》1997,103(4):323-329

Brazilian isolates of Colletotrichum spp. from citrus orchards affected by postbloom fruit drop were examined for colony colour, mycelial growth, benomyl-resistance, pathogenicity, and genetic variability by random amplified polymorphic DNA (RAPD) analysis. All isolates were obtained from flowers and persistent calyxes from different citrus hosts from Sao Paulo, Brazil. DNA polymorphisms detected after amplification with random 10-mer primers were used to classify the isolates into two groups. Group I isolates grew rapidly on potato-dextrose agar (PDA) and were sensitive to benomyl, and group II isolates grew slowly on PDA and were benomyl-resistant. Colletotrichum acutatum was analyzed by RAPD and had high genetic similarity with group II isolates of Colletotrichum from citrus. Probably, the group I is C. gloeosporioides and group II is C. acutatum.  相似文献   

Seasonal influence on hematologic values and hemoglobin electrophoresis in Brazilian boa constrictor amarali.     

Carla C Machado  Luis F N Silva  Paulo R R Ramos  Regina K Takahira 《Journal of zoo and wildlife medicine》2006,37(4):487-491

As ectothermic animals, snakes depend exclusively on the environment for proper temperature maintenance, which may greatly influence their activity. Twenty-five adult Boa constrictor amarali snakes maintained in captivity were used to determine the influence of seasons on their hematologic values and electrophoretic profile of hemoglobin. A complete blood cell count (CBC) and examination for hemoparasites were performed in the summer and winter of 2004. Hemoglobin was stored for later electrophoresis. Significant differences (P < 0.05) were obtained in RBC, WBC, lymphocyte, thrombocyte, and monocyte counts, demonstrating the importance of the period of the year in the interpretation of reference values in these animals. Two snakes were detected with blood parasites (Hepatozoon sp.) in the winter and four in the summer, although it appears that their presence did not cause any significant alterations in the CBC. The electrophoretic analysis of the samples demonstrated two-four hemoglobin bands in this species.  相似文献   

Development of a Suitable Maintenance Diet for Anodonta cygnea     

Fátima  Carvalho Paula  Lima Fernando  Gonçalves Fernanda  Russell-Pinto Jorge  Machado 《Journal of the World Aquaculture Society》2004,35(2):189-198

Selenastrum, Chlorella and the Cyanophyta groups, important components of the phytoplankton in the natural habitat of Anodonta cygnea in Mira Lagoon, were used separately as experimental diet for Anodonta cygnea. To assess the effects of the different diets, several organic and inorganic compounds of the haemolymph and extrapallial fluid were measured. Protein content was significantly reduced with experimental Non Feed, and carbohydrate content with Non Feed, Selenastrum, Chlorella , and Cyanophyta diets compared to the control in the lagoon. Lipid contents in body fluids were similar among the different diet groups, with the exception of the Chlorella diet, which showed a higher value. In all diets, the inorganic elements, magnesium and chloride, presented a non-significant variation profile. The concentration of sodium and potassium was significantly affected in Selenastrum and Chlorella diets, observing in the Non Feed diet a minimum value for potassium. The non-significant variation of osmolality suggests the presence of a specific osmotic regulatory mechanism independent of the diet. A particular regulation mechanism seems to exist for both calcium and manganese ion inducing similar profiles for all experimental diets with higher contents in Selenastrum and Cyanophyta diets, respectively. The higher calcium ion concentration appears associated with lower protein-carbohydrate contents, probably due to weak calcium aggregation and deposition mechanism, except for the control and Mixture diets. In general, the concentrations of organic and inorganic compounds in the body fluids of Mixture-fed A. cygnea , were more similar to those found in the control diet group. This suggests that an equal mixture of the three algae, Selenastrum, Chlorella , and Cyanophyta, is more appropriate diet to provide the necessary nutrients for a normal maintenance of A. cygnea in the wild.  相似文献   

Soil biological properties in multistrata successional agroforestry systems and in natural regeneration     

Raul Matias Cezar  Fabiane Machado Vezzani  Daniel Kramer Schwiderke  Sérgio Gaiad  George Gardner Brown  Carlos Eduardo Sícoli Seoane  Luís Cláudio Maranhão Froufe 《Agroforestry Systems》2015,89(6):1035-1047

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Identification,occurrence and clinical findings of canine hemoplasmas in southern Brazil     

Stella de Faria Valle  Joanne B. Messick  Andrea Pires dos Santos  Luiz Carlos Kreutz  Naila Cristina Blatt Duda  Gustavo Machado  Luis Gustavo Corbellini  Alexander Welker Biondo  Felix Hilario Diaz González 《Comparative immunology, microbiology and infectious diseases》2014

Hemoplasmas are ubiquitous pleomorphic and epicellular bacteria detected in erythrocytes in several species. In Brazil, studies on hemoplasmas have not included information on occurrence, clinical signs, and risk factors in dogs. This paper investigates the occurrence of hemoplasmas in dogs, focusing on risk factors and clinical status. Conventional PCR for the four types of canine hemoplasmas was performed in 331 blood samples collected from dogs clinically treated at a teaching veterinary hospital. Of all samples, 17/331 (5.1%) were positive for Mycoplasma haemocanis and 6/331 (1.8%) were positive for a ‘Candidatus Mycoplasma haemominutum-like’ organism. Risk factors included the presence of vectors, old age, dog bite wounds, and neoplastic diseases. In the multivariate analysis, a 4.40 odds ratio in dogs with vector-borne diseases indicated risk for hemoplasmosis. There was correlation between hemoplasma infection and neoplastic disease, suggesting that neoplastic conditions are a risk factor for hemoplasma infection in dogs.  相似文献   

Relevance of synovial fluid chondroitin sulphate as a biomarker to monitor polo pony joints     

Raquel Y.A. Baccarin  Luciane Rasera  Thaís S.L. Machado  Yara M. Michelacci 《Canadian journal of veterinary research》2014,78(1):50-60

Osteoarthritis (OA) of the metacarpophalangeal joint is the most common articular disease in polo ponies leading to early retirement. A biomarker that would discriminate between pathological and physiological changes secondary to exercise could be helpful in OA prevention. The aim of this study was to investigate the effects of polo training on synovial fluid biomarkers of inflammation and cartilage turnover in polo ponies of different skill levels. Synovial fluid samples were collected from metacarpophalangeal joints of polo ponies before and during the polo season (320 d). Nucleated cells, soluble protein, prostaglandin E₂ (PGE₂), glycosaminoglycans (GAG), and urea were measured. The main synovial fluid GAG are chondroitin sulphate (CS, ~25 μg/mL) and hyaluronic acid (HA, ~400 μg/mL). After a polo match, a transitory increase in protein and PGE₂, but not CS and HA, occurred (expressed as urea ratio), returning to basal levels in 24 h. During the polo season, the number of synovial fluid nucleated cells was always in the normal range. Increases in protein and HA occurred during the initial 40 to 80 d, returning to basal levels afterwards. In contrast, in polo prospects the concentration of CS steadily increased during the season. Long-term follow-up revealed that the synovial fluid CS was significantly higher in polo ponies that developed joint diseases within 24 months following our study. In conclusion, CS seems to be an early marker of articular cartilage damage.  相似文献   

Hereditary renal disease in a mutant strain of rats   总被引：4，自引：0，他引：4  

B B Lozzio  A I Chernoff  E R Machado  C B Lozzio 《Science (New York, N.Y.)》1967,156(783):1742-1744

Disease of the kidney developed in breeding stock of Gunn rats. The renal lesion is the result of a new mutation. The genetic defect is inherited as an autosomal dominant trait and is apparently lethal in the homozygous condition. The abnormality manifests itself as a congenital hydronephrosis with related cystic changes in the kidney.  相似文献