全文获取类型
收费全文 | 225篇 |
免费 | 11篇 |
专业分类
林业 | 23篇 |
农学 | 12篇 |
基础科学 | 1篇 |
77篇 | |
综合类 | 12篇 |
农作物 | 16篇 |
水产渔业 | 8篇 |
畜牧兽医 | 66篇 |
园艺 | 5篇 |
植物保护 | 16篇 |
出版年
2023年 | 3篇 |
2022年 | 7篇 |
2021年 | 9篇 |
2020年 | 10篇 |
2019年 | 12篇 |
2018年 | 20篇 |
2017年 | 10篇 |
2016年 | 14篇 |
2015年 | 6篇 |
2014年 | 15篇 |
2013年 | 12篇 |
2012年 | 23篇 |
2011年 | 18篇 |
2010年 | 5篇 |
2009年 | 9篇 |
2008年 | 11篇 |
2007年 | 3篇 |
2006年 | 14篇 |
2005年 | 6篇 |
2004年 | 7篇 |
2003年 | 5篇 |
2002年 | 5篇 |
2001年 | 1篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 3篇 |
1996年 | 1篇 |
1994年 | 2篇 |
1992年 | 1篇 |
1987年 | 1篇 |
排序方式: 共有236条查询结果,搜索用时 15 毫秒
121.
Pavlova B Volf J Ondrackova P Matiasovic J Stepanova H Crhanova M Karasova D Faldyna M Rychlik I 《Veterinary research》2011,42(1):16
ABSTRACT: Genes localized at Salmonella pathogenicity island-1 (SPI-1) are involved in Salmonella enterica invasion of host non-professional phagocytes. Interestingly, in macrophages, SPI-1-encoded proteins, in addition to invasion, induce cell death via activation of caspase-1 which also cleaves proIL-1β and proIL-18, precursors of 2 proinflammatory cytokines. In this study we were therefore interested in whether SPI-1-encoded type III secretion system (T3SS) may influence proinflammatory response of macrophages. To test this hypothesis, we infected primary porcine alveolar macrophages with wild-type S. Typhimurium and S. Enteritidis and their isogenic SPI-1 deletion mutants. ΔSPI1 mutants of both serovars invaded approx. 5 times less efficiently than the wild-type strains and despite this, macrophages responded to the infection with ΔSPI1 mutants by increased expression of proinflammatory cytokines IL-1β, IL-8, TNFα, IL-23α and GM-CSF. Identical macrophage responses to that induced by the ΔSPI1 mutants were also observed to the infection with sipB but not the sipA mutant. The hilA mutant exhibited an intermediate phenotype between the ΔSPI1 mutant and the wild-type S. Enteritidis. Our results showed that the SPI-1-encoded T3SS is required not only for cell invasion but in macrophages also for the suppression of early proinflammatory cytokine expression. 相似文献
122.
Jolanta Floryszak-Wieczorek Zbigniew Górski Magdalena Arasimowicz-Jelonek 《European journal of plant pathology / European Foundation for Plant Pathology》2011,130(2):249-258
Each living cell of a plant produces photons in certain conditions. Under normal physiological conditions, cell photon emission
is stationary and minimal. Disturbance in the oxidative homeostasis by biotic stress is manifested by increased ‘biophoton’
production. Such biophoton responses of plants may be used as an integral indicator of the degree of oxidative homeostasis
misbalance. Our results demonstrate that biophoton generation has been much higher in a resistant potato variety than in a
susceptible one till 10 h after Phytophthora infestans inoculation. In contrast, ultra-weak luminescence from detached susceptible potato and moderately resistant pelargonium leaves
increased from 1–4 to 4–5 days after inoculation with Phytophthora infestans or Botrytis cinerea, respectively. Pre-treatment of susceptible potato leaves with a defence inducer, arachidonic acid, resulted in a transient
burst of light in response to P. infestans lasting for 30–45 h post inoculation (hpi). This study presents the potential adaptation of functional imaging of ultra-weak
luminescence to monitor time-dependent free radical processes during disease development and its application to draw conclusions
on plant resistance to pathogens of different lifestyle. Moreover, it has been shown that imaging of temporal biophoton generation
from potato leaves treated with arachidonic acid might be a helpful marker in mapping oxidative changes leading to systemic
acquired resistance (SAR). 相似文献
123.
Anna Trojak-Goluch Dorota Laskowska Monika Agacka Diana Czarnecka Magdalena Kawka Anna Czubacka 《Breeding Science》2011,61(4):389-393
Black root rot (BRR) caused by Thielaviopsis basicola as well as Tomato spotted wilt virus (TSWV) are the most serious problems in tobacco growing regions. We crossed the breeding line WGL 3 carrying BRR resistance derived from N.glauca with the line PW-834 the resistance of which to TSWV was transferred from cultivar Polalta. Anthers obtained from F1 hybrid plants were cultured to induce haploids combining resistance to Th. basicola and TSWV. Flow cytometry analysis revealed 242 haploids and 2 spontaneous doubled haploids among regenerants. All haploids were cloned and then evaluated for BRR as well as TSWV resistance. The presence of pathogens was detected by microscopic evaluation of roots or using DAS-ELISA test. Microscopic assessment showed that, 132 haploids had no symptoms of Th. basicola which, together with the absence of symptoms in the F1 hybrids, indicated a dominant monogenic mode of inheritance. At the same time only 30 haploids demonstrated resistance to TSWV. SCAR markers associated with TSWV resistance gene detection was applied. The results indicate that small proportion of TSWV-resistant haploids is probably due to the influence of deleterious genes flanking the resistance factor that reduced vitality of gametophytes. Altogether, 24 haploids showed multiple resistance to Th. basicola and TSWV. 相似文献
124.
Baran Agnieszka Klimkowicz-Pawlas Agnieszka Ukalska-Jaruga Aleksandra Mierzwa-Hersztek Monika Gondek Krzysztof Szara-Bąk Magdalena Tarnawski Marek Spałek Iwona 《Journal of Soils and Sediments》2021,21(6):2418-2431
Journal of Soils and Sediments - The aims of the study were to investigate the interaction between fractions of organic matter and polycyclic aromatic hydrocarbons (PAHs) in bottom sediments and to... 相似文献
125.
Mikuláscheck Madaras Markéta Mayerová Martin Kulhánek Magdalena Koubová Miloš Faltus 《Archives of Agronomy and Soil Science》2013,59(5):671-683
After 1989, the use of potassium (K) mineral fertilizers in the Czech Republic dropped from ~55 kg K ha?1 to a mean rate of ~6.5 kg in the last decade. In order to test alternative solutions for K fertilization, the trioctahedral mica mineral zinnwaldite (8% K), orthoclase (10% K) and waste mica from Cínovec (Kru?né Hory Mts/Erzgebirge, Czech Republic), consisting primarily of zinnwaldite, were applied as the only K sources for spring barley. The minerals were treated in three different types of high-energy mills under different working conditions. Application rates in the range 139–820 mg K kg?1 were tested in quartz sand cultures. In all treatments, plant growth, total plant biomass and the K content in the plant tissues increased in the order zinnwaldite > waste mica > orthoclase. K fractionation and K plant uptake were significantly influenced by the milling method used. The effect of 195 mg K kg?1 as zinnwaldite on K uptake from K-depleted soils was positive; however, it was smaller than in sand cultures because of the relatively high content of non-exchangeable K in the soils. Direct use of waste mica as a K fertilizer is limited by the increased fluorine and heavy metal content. 相似文献
126.
Richard Lumu Constantine Bakyusa Katongole Justine Nambi-Kasozi Felix Bareeba Magdalena Presto Emma Ivarsson Jan Erik Lindberg 《Tropical animal health and production》2013,45(7):1571-1578
This study identified the indigenous criteria used by livestock farmers in urban and peri-urban areas of Kampala to assess the nutritional quality of available feed resources. Focus group discussions and questionnaire interviews (with a total of 120 livestock farming households) were conducted. The findings showed that banana peels, leftover food and own-mixed feeds were the most commonly used feed resources for cattle, pigs and chickens, respectively. Farmers use several indigenous criteria to judge the nutritional quality of the available feed resources. These included perceived effects on disease resistance, feed intake, growth/body condition, hair coat appearance, faecal output, faecal texture and level of production, among others. According to farmers, animals offered with a feed resource of good nutritional quality are more resistant to diseases, ingest much of the feed, gain weight with well-filled bodies, have smooth hair coats, produce large quantities of faeces that are not too firm or watery and exhibit good performance (lactating cows produce more milk, sows produce piglets of good body size, hens lay more eggs of normal size, etc.). Although this indigenous knowledge exists, farmers put more importance on availability and cost as opposed to nutritional quality when choosing feed resources. This explains why banana peels were among the feed resources perceived to be of low nutritional quality but, at the same time, were found to be the most commonly used. Hence, there is a need to sensitise farmers on the importance of nutritional quality in ensuring better and efficient utilisation of the available feed resources. 相似文献
127.
Magdalena Garncarz Marta Parzeniecka-Jaworska Micha? Jank Magdalena ?ój 《Acta veterinaria Scandinavica》2013,55(1):52
Background
Chronic mitral valve disease is frequently seen in the Dachshund. Dachshunds (n=207) made up 11.73% of the dogs admitted to the Cardiology Service at the Small Animal Clinic, Warsaw University of Life Sciences, Poland (first visits only).Results
Of these, 35 dogs had no clinically detectable heart disease while 172 had chronic valve disease with the mitral valve affected most often (130 dogs), both mitral and tricuspid valves infrequently (39 dogs) and rarely the tricuspid valve (3 dogs). Males were affected more frequently than females and the average age of dogs with chronic valve disease was 11.9 years for females and 11.3 years for males. A majority of the diseased Dachshunds were classified as ISACHC 2 (79), followed by ISACHC 1 (60). Most frequent clinical signs noted by owners included coughing, exercise intolerance, dyspnea and tachypnea. Heart murmurs were generally louder with increased disease severity; however there were 20 dogs in the ISACHC 1 group with no audible heart murmurs. The most frequent electrocardiographic abnormalities included an increased P wave and QRS complex duration, increased R wave amplitude and tachycardia. With increased disease severity, echocardiography revealed an increase in heart size. A higher ISACHC class was related to increased heart size (based on echocardiography) and increased percentage of patients exhibiting enlargement of both left atrium and left ventricle (based on radiography).Conclusions
The Dachshund is often affected by chronic mitral valvular disease with a late onset of associated clinical signs and few cardiac complications. 相似文献128.
129.
The aim of this study was to investigate the impact of carbofuran, a carbamate pesticide on the enzymatic activity of acetylcholinesterase (AChE) in the larvae of the herring nematode parasite Anisakis simplex. A. simplex larvae collected from herring were exposed to carbofuran in vivo at concentrations of 50, 100, 500 and 1000 μg/l, for 24, 48 and 72 h, at a temperature of 4 °C. Generalized Linear Models (GLM) were applied to analyze the relationship between AChE activity and carbofuran concentration, the time of exposure and the biological parameters of the host. The results indicate that A. simplex larvae have a high threshold of sensitivity to carbofuran. The average enzymatic activity was higher in parasites obtained from male hosts, when compared with female hosts. These data suggest that host sex-dependent biological processes may also influence AChE enzymatic activity in parasites. 相似文献
130.
Sylwia Magdalena Okoń Tomasz Ociepa 《European journal of plant pathology / European Foundation for Plant Pathology》2017,149(3):711-718
The aim of the present study was to determine the virulence structure of powdery mildew of oats (Blumeria graminis DC.f. sp. avena) in Poland in the years 2010–2013. For this purpose, powdery mildew isolates were collected from three experimental stations in Poland. To assess the virulence of the isolates, eight oat varieties with different responses to the pathogen were used. The results showed that a significant proportion of powdery mildew isolates found in Poland overcame the resistance genes of varieties Bruno (Pm6), Jumbo (Pm1) and Mostyn (Pm3). In contrast, lines Av1860 (Pm4), Am27 (Pm5) and Cc3678 (Pm2) were completely resistant to all pathogen isolates involved in the experiment. Changes constantly occurring in the powdery mildew population perfectly reflect diversity indexes, which were the smallest in the first year of observation, where in the following years these parameters were significantly higher. It is worth noting that the presence of powdery mildew is seasonal and local, which is reflected in the prevalence of the disease in a defined area of the country. 相似文献