Indoor activity of Culicoides associated with livestock in the bluetongue virus (BTV) affected region of northern France during autumn 2006   总被引：4，自引：1，他引：3  

T. Baldet  J.C. Delcolle  C. Cêtre-Sossah  B. Mathieu  R. Meiswinkel  G. Gerbier 《Preventive veterinary medicine》2008,87(1-2):84

In August 2006, bluetongue virus (BTV) was detected in the Netherlands, Belgium, western Germany, Luxembourg and northern France for the first time. Consequently, a longitudinal entomological study was conducted in the affected region of northern France (Ardennes) throughout the autumn of 2006. Data on the spatio-temporal distribution of Culicoides (Diptera: Ceratopogonidae) associated with livestock were collected and an attempt was made to identify the vector(s) involved in BTV transmission by means of virus detection in wild-caught biting midges. Weekly sampling using standardized Onderstepoort-type blacklight traps were performed simultaneously both outdoors and indoors in one BTV-free and three BTV-affected farms between September and December 2006.Culicoides were sorted according to farm, location (outdoors vs. indoors), time point (in weeks), species and physiological stage. BTV detection was conducted by RT-PCR on monospecific pools of non-bloodfed parous female Culicoides.The principal results showed: (i) the absence of the Mediterranean vector, C. imicola, (ii) the relatively low abundance of C. dewulfi and C. pulicaris, (iii) the widespread occurrence and abundance of C. obsoletus/C. scoticus with longevity and behaviour compatible with BTV transmission, and (iv) all Culicoides pools tested for BTV were negative.In France, the very low levels of BTV-8 circulation were probably due to the limited introduction of the virus from affected neighbouring countries, and not due to the absence of local vector populations. A key finding has been the substantiation, for the first time, that Culicoides, and particularly the potential vectors C. obsoletus/C. scoticus and C. dewulfi, can be active at night inside livestock buildings and not only outside, as originally believed.The endophagic tendencies of members of the Obsoletus group are discussed in light of the prolonged period of BTV transmission during the autumn of 2006 and the risk of BTV overwintering and resurgence in the spring of 2007. Overall, there is an urgent need to improve our knowledge on the ecology of local Culicoides species before any clear, effective and reliable recommendations can be provided to the veterinary authorities in terms of prevention and control.  相似文献   

Laboratory Evaluation of Flight Capacities of Aedes japonicus (Diptera: Culicidae) Using a Flight Mill Device     

Eva Krupa  Alexa-Lou Grhal  Jrmy Esnault  Christelle Bender  Bruno Mathieu 《Journal of insect science (Online)》2021,21(6)

Dispersion expands the distribution of invasive species and as such, it is a key factor of the colonization process. Aedes japonicus japonicus (Theobald, 1901) is an invasive species of mosquito and a vector of various viruses. It was detected in the northeast of France in 2014. The population of this species can expand its distribution by several kilometers per year. However, though flight capacities play an active part in the dispersion of Ae. japonicus, they remain unknown for this species. In this study, we investigated the flight capacities of Ae. japonicus in a laboratory setting using the flight mill technique. We evaluated the influence of age on flight. We recorded videos of individual flights with a camera mounted on Raspberry Pi. We extracted data on distance, duration, and speed of flight using the Toxtrac and Boris software. Our analysis showed a median flight distance of 438 m with a maximum of 11,466 m. Strong flyers, which represented 10% of the females tested, flew more than 6,115 m during 4 h and 28 min at a speed of 1.7 km per h. As suspected, Ae. japonicus is a stronger flyer than the other invasive species Aedes albopictus (Skuse, 1894) (Diptera: Culicidae). To our knowledge, this is the first flight mill study conducted on Ae. japonicus and therefore the first evaluation of its flight capacity. In the future, the flight propensity of Ae. japonicus determined in this study can be included as a parameter to model the colonization process of this invasive vector species.  相似文献   

Effect of feeding scheme and prey density on survival and development of Chinese mitten crab Eriocheir sinensis zoea larvae     

Li-ying Sui  Mathieu Wille  Xu-gan Wu  Yong-xu Cheng  & Patrick Sorgeloos 《Aquaculture Research》2008,39(6):568-576

The effect of feeding scheme and prey density on survival and development of Eriocheir sinensis zoea larvae was studied in three experiments. Different combinations and densities of rotifers (Brachionus rotundiformis) and newly hatched Artemia nauplii were fed to zoea larvae. Average survival at each stage, larval development (larval stage index, LSI), duration of zoeal stage and individual megalopa dry weight were compared among treatments. This study revealed that, under the experimental conditions, rotifers should be replaced with Artemia between the zoea 3 (Z3) and the zoea 4 (Z4) stage. The optimal rotifer feeding densities for zoea 1 (Z1) and zoea 2 (Z2) were 15 and 20 mL^?1 respectively, while the optimal Artemia feeding density for Z3, Z4 and zoea 5 (Z5) was 3, 5 and 8 mL^?1 respectively. Further trials in production scale are recommended.  相似文献   

Development of a 1-step enzyme-linked immunosorbent assay for the rapid diagnosis of bovine respiratory syncytial virus in postmortem specimens.     

Birgit Quinting  Béatrice Robert  Carine Letellier  Mathieu Boxus  Pierre Kerkhofs  Frédéric Schynts  Alfred Collard 《Journal of veterinary diagnostic investigation》2007,19(3):238-243

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Experimental study on the toxicity of imidacloprid given in syrup to honey bee (Apis mellifera) colonies     

Faucon JP  Aurières C  Drajnudel P  Mathieu L  Ribière M  Martel AC  Zeggane S  Chauzat MP  Aubert MF 《Pest management science》2005,61(2):111-125

Two groups of eight honey bee colonies were fed with two different concentrations of imidacloprid in saccharose syrup during summer (each colony was given 1 litre of saccharose syrup containing 0.5 microg litre(-1) or 5 microg litre(-1) of imidacloprid on 13 occasions). Their development and survival were followed in parallel with control hives (unfed or fed with saccharose syrup) until the end of the following winter. The parameters followed were: adult bee activity (number of bee entering the hive and pollen carrying activity), adult bee population level, capped brood area, frequency of parasitic and other diseases, mortality, number of frames with brood after wintering and a global score of colonies after wintering. The only parameters linked to feeding with imidacloprid-supplemented saccharose syrup when compared with feeding with non-supplemented syrup were: a statistically non-significant higher activity index of adult bees, a significantly higher frequency of pollen carrying during the feeding period and a larger number of capped brood cells. When imidacloprid was no longer applied, activity and pollen carrying were re-established at a similar level for all groups. Repeated feeding with syrup supplemented with imidacloprid did not provoke any immediate or any delayed mortality before, during or following the next winter, whereas such severe effects are described by several French bee keepers as a consequence of imidacloprid use for seed dressing in neighbouring cultures. In any case, during the whole study, mortality was very low in all groups, with no difference between imidacloprid-fed and control colonies. Further research should now address several hypotheses: the troubles described by bee keepers have causes other than imidacloprid; if such troubles are really due to this insecticide, they may only be observed either when bees consume contaminated pollen, when no other sources of food are available, in the presence of synergic factors (that still need to be identified), with some particular races of bees or when colonies are not strong and healthy.  相似文献   

Etude de la Teneur en Cobalt dans l''Alimentation des Bovins de Diverses Régions du Québec          下载免费PDF全文

L. G. Mathieu  R. Dufour 《The Canadian veterinary journal. La revue veterinaire canadienne》1965,6(5):112-114

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Glossopharyngeal Insufflation and Breath-Hold Diving: The More,the Worse?     

Alain Boussuges  Olivier Gavarry  Jacques Bessereau  Mathieu Coulange  Morgan Bourc’his  Pascal Rossi 《The Forestry Chronicle》2014,25(4):466-471

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Silica-like malleable materials from permanent organic networks     

Montarnal D  Capelot M  Tournilhac F  Leibler L 《Science (New York, N.Y.)》2011,334(6058):965-968

Permanently cross-linked materials have outstanding mechanical properties and solvent resistance, but they cannot be processed and reshaped once synthesized. Non-cross-linked polymers and those with reversible cross-links are processable, but they are soluble. We designed epoxy networks that can rearrange their topology by exchange reactions without depolymerization and showed that they are insoluble and processable. Unlike organic compounds and polymers whose viscosity varies abruptly near the glass transition, these networks show Arrhenius-like gradual viscosity variations like those of vitreous silica. Like silica, the materials can be wrought and welded to make complex objects by local heating without the use of molds. The concept of a glass made by reversible topology freezing in epoxy networks can be readily scaled up for applications and generalized to other chemistries.  相似文献   

Radiological prevalence of osteoarthritis of the cervical region in 104 performing Warmblood jumpers     

Pablo Espinosa-Mur  Kathryn L. Phillips  Larry D. Galuppo  Anthony DeRouen  Philippe Benoit  Eleanor Anderson  Karen Shaw  Sarah Puchalski  Duncan Peters  Philip H. Kass  Mathieu Spriet 《Equine veterinary journal》2021,53(5):972-978

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Soil macrofaunal biodiversity in Amazonian pastures: Matching sampling with patterns     

Jean-Pierre Rossi  Jérôme Mathieu  Michel Grimaldi 《Soil biology & biochemistry》2006,38(8):2178-2187

Soil biodiversity varies through space as influenced by habitat features and land-use history. The performance of any sampling strategy highly depends on its relevance with regards to this pattern. We surveyed the soil macrofaunal species richness in the pastures of the Benfica Field Station (Eastern Amazonia, State of Pará, Brazil) and described its variability in 4 independent replicate plots. We designed a within-plot sampling scheme that accounted for the soil spatial variation (stratified sampling). Replicated pasture plots had different species richness (49-65) corresponding to a low proportion (40-53%) of the total number of species (123). Pairs of replicated plots showed an outstandingly low number of shared species (28-41% of the species pool). Likewise, different classes of soil thickness, corresponding to a Ferralsol-Cambisol sequence, had different species richness (12-44) and exhibited a very low proportion of shared species (15-29%). The proportion of rare species, i.e. singletons, ranged from 40-51% of the total species richness depending on the plot considered. We used the abundance-based coverage estimator of species richness (ACE) and the Chao shared species estimator that provides a correction based on the relative abundance of rare species. These indices also showed both a high between plots dissimilarity and a substantial within plot variability of species composition. Because of the high proportion of rare species, the rarefaction curves failed to reach any asymptote in all replicated plots. Bootstrap resampling showed that less than 5 samples per stratum (class of soil thickness) provided inconsistent species richness values. We simulated the efficiency of sampling strategies that included our 4 replicate plots and the 3 classes of soil thickness but with varying sampling effort within each stratum. The results indicated that a fairly large (74%) proportion of species would be recorded if strata were sampled using 5 sampling units (hence 15 samples per plot for a total of 4×15=60 samples). This study showed the need for adequate plot replication in soil macrofaunal biodiversity studies. Also, the main relevant factors of within-replicate plot spatial heterogeneity (e.g. soil, vegetation) should be accounted for through stratified sampling. The results showed that there is no way of reducing the local sampling effort below a certain level (here, 5 sampling units per stratum).  相似文献