Postoperative Respiratory Function and Survival After Pneumonectomy in Dogs and Cats          下载免费PDF全文

Stephanie A. Majeski  Michele A. Steffey  Philipp D. Mayhew  Geraldine B. Hunt  David E. Holt  Jeffrey J. Runge  Philip H. Kass  Matthew Mellema 《Veterinary surgery : VS》2016,45(6):775-781

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POSITRON EMISSION TOMOGRAPHY FEATURES OF CANINE NECROTIZING MENINGOENCEPHALITIS     

KI‐DONG EOM  CHAE‐YOUNG LIM  SU‐HYUN GU  BYEONG‐TECK KANG  YOUNG‐BO KIM  DONG‐PYO JANG  EUNG‐JE WOO  DAEYOUNG KIM  ZANG‐HEE CHO  HEE‐MYUNG PARK 《Veterinary radiology & ultrasound》2008,49(6):595-599

A Yorkshire terrier and a Chihuahua were referred for acute onset, generalized tonic‐clonic seizures and were suspected to have meningoencephalitis based on magnetic resonance (MR) imaging findings. Brain lesions appeared hyperintense with T2‐weighted imaging and hypointense with T1‐weighted imaging, and were characteristic of necrotizing meningoencephalitis. Both dogs were diagnosed with necrotizing meningoencephalitis based on pathologic findings. Fluorine‐18 fluorodeoxyglucose positron emission tomography (FDG‐PET) was performed on both animals before euthanasia with the permission of the owner. In FDG‐PET images, these lesions seen in MR images were characterized by multifocal or diffuse hypometabolism. Our FDG‐PET results provided evidence of glucose hypometabolism in areas of necrosis and cavitation associated with necrotizing meningoencephalitis. FDG‐PET has the potential to provide valuable diagnostic information in dogs with suspected necrotizing encephalitis.  相似文献   

Perioperative morbidity and mortality in dogs with invasive adrenal neoplasms treated by adrenalectomy and cavotomy     

Philipp D. Mayhew  Sarah E. Boston  Allison L. Zwingenberger  Michelle A. Giuffrida  Jeffrey J. Runge  David E. Holt  Joseph S. Raleigh  Ameet Singh  William T. N. Culp  J. Brad Case  Michele A. Steffey  Ingrid M. Balsa 《Veterinary surgery : VS》2019,48(5):742-750

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Hypernatraemia in 39 hospitalised foals: clinical findings,primary diagnosis and outcome          下载免费PDF全文

NM Collins  JB Carrick  CM Russell  JE Axon 《Australian veterinary journal》2018,96(10):385-389

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Prospective evaluation of outcome of dogs with intrahepatic portosystemic shunts treated via percutaneous transvenous coil embolization          下载免费PDF全文

William T. N. Culp VMD  DACVS  Allison L. Zwingenberger DVM  MAS  DACVR  Michelle A. Giuffrida VMD  DACVS  Erik R. Wisner DVM  DACVR  Geraldine B. Hunt BVSc  PhD  Michele A. Steffey DVM  DACVS  Philipp D. Mayhew BVM'S  DACVS 《Veterinary surgery : VS》2018,47(1):74-85

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Evaluation of single port access gastropexy and ovariectomy using articulating instruments and angled telescopes in dogs     

Jeffrey J. Runge DVM  Diplomate ACVS  Philipp D. Mayhew BVM&S  Diplomate ACVS 《Veterinary surgery : VS》2013,42(7):807-813

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Efficiency of Two Enucleation Methods Connected to Handmade Cloning to Produce Transgenic Porcine Embryos     

J Li  K Villemoes  Y Zhang  Y Du  PM Kragh  S Purup  Q Xue  AM Pedersen  AL Jørgensen  JE Jakobsen  L Bolund  H Yang  G Vajta 《Reproduction in domestic animals》2009,44(1):122-127

The purpose of our work was to establish an efficient-oriented enucleation method to produce transgenic embryos with handmade cloning (HMC). After 41–42 h oocytes maturation, the oocytes were further cultured with or without 0.4 μg/ml demecolcine for 45 min [chemically assisted handmade enucleation (CAHE) group vs polar body (PB) oriented handmade enucleation (OHE) group respectively]. After removal of the cumulus cells and partial digestion of the zona pellucida, oocytes with visible extrusion cones and/or polar bodies attached to the surface were subjected to oriented bisection. Putative cytoplasts without extrusion cones or PB were selected as recipients. Two cytoplasts were electrofused with one transgenic fibroblasts expressing green fluorescent protein (GFP), while non-transgenic fibroblasts were used as controls. Reconstructed embryos were cultured in Well of Wells (WOWs) with porcine zygote medium 3 (PZM-3) after activation. Cleavage and blastocyst rates were registered on day 2 and day 7 of in vitro culture respectively. Meanwhile, the total blastocyst cell number was counted on day 7. We found that the difference was only observed between blastocyst rates (38.6 ± 2% vs 48.1 ± 3%) of cloned embryos with GFP transgenic fibroblast cells after CAHE vs OHE. With adjusted time-lapse for zonae-free cloned embryos cultured in WOWs with PZM-3, it was obvious that in vitro developmental competence after CAHE was compromised when compared with the OHE method. OHE enucleation method seems to be a potential superior alternative method used for somatic cell nuclear transfer (SCNT) with transgenic fibroblast cells.  相似文献   

Effects of Matrix Filtration of Low-Quality Boar Semen Doses on Sperm Quality     

E Bussalleu  E Pinart  MM Rivera  M Briz  S Sancho  M Yeste  I Casas  A Fàbrega  T Rigau  JE Rodriguez-Gil  S Bonet 《Reproduction in domestic animals》2009,44(3):499-503

The aim of this work was to develop a method to enhance the sperm parameters of ejaculates with low sperm quality from Piétrain boars. Seminal doses were filtered through columns of DEAE Sephadex (length 2.5 ± 0.5 cm), CM Sephadex (length 5 ± 0.5 cm), glass wool (length 2 ± 0.5 cm) or glass bead (length 10 ± 0.5 cm), with an exit flow rate of 1 ml/40 s in all cases. For each male, 10 ml of the sperm cell-rich fraction diluted at 1 : 6 were filtered. Sperm quality was assessed before and after filtration. Sperm morphology, sperm motility and sperm concentration were determined using the computer program sca ^® 2002 Production, and sperm viability was evaluated by fluorescence multistaining. Osmotic resistance test and hyperosmotic resistance test were used to determine the osmotic resistance of spermatozoa, whereas l -lactate production estimated the metabolic activity. Results showed a decrease of sperm concentration and osmotic resistance of spermatozoa after filtration in the four matrixes. However, an increase in the frequency of viable spermatozoa with intact acrosome after filtration in glass bead columns and an increase of morphologically normal spermatozoa after filtration in Sephadex CM-50, glass wool and glass bead columns were observed. Despite the decrease in the frequency of progressive motile spermatozoa, l -lactate production and mitochondrial sheath integrity maintained constant after filtration. Our findings indicate that column filtration is an effective method to enhance the sperm quality by selecting viable and morphologically normal spermatozoa without altering DNA, plasma membrane, mitochondrial sheath integrity or inducing premature acrosome reaction.  相似文献   

Cervical vertebral fractures     

I. G. Joe Mayhew 《Equine Veterinary Education》2009,21(10):536-539

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The distribution of nerve fibres immunoreactive for vasoactive intestinal peptide, calcitonin gene-related peptide, substance P and dopamine beta-hydroxylase in the normal equine larynx   总被引：2，自引：0，他引：2  

Corcoran BM  Jarvis S  Hahn CN  Mayhew IG 《Research in veterinary science》1999,67(3):251-259

The autonomic innervation of the mammalian respiratory system is complex, and involves a wide variety of peptide and non-peptide neurotransmitters which will have an important role in normal laryngeal function and the response to disease. This innervation has been partially described in the horse airway and lung, but there is no information on the equine larynx. This paper describes the expression and distribution of nerve fibres immunoreactive for vasoactive intestinal peptide (VIP), calcitonin gene-related peptide (CGRP), substance P (SP) and the adrenergic enzymatic marker dopamine beta-hydroxylase (DBetaH) in the mucosa of the equine larynx. The overall relative density of nerve fibres immunoreactive for the different antigens was VIP>CGRP>SP>DBetaH. There were differences in the distribution of nerve fibre types, although each antigen was found in nerve fibres adjacent to blood vessels and mucous glands. VIP -like immunoreactivity (VIP -Li) was particularly extensive in association with mucous glands. SP - and CGRP -like immunoreactivity (SP -Li, CGRP -Li) were also seen close to the epithelium, with occasional nerve fibres coursing beneath and between the epithelial cells. Fragments of SP -Li and CGRP -Li fibres were also present in large nerve fibre bundles and ganglionic cell clusters, but not in the neurons themselves. The density of nerve fibres immunoreactive for DBetaH was very low and restricted to blood vessels and mucous glands. There was marked variation in the density of nerve fibres at the different sites, with the greatest density, particularly for VIP, over the arytenoid cartilage. Immunoreactive nerve fibres were less plentiful over the epiglottis, and the density of all types of nerve fibres was low over the cricoid cartilage. Overall VIP -Li nerve fibres were the most plentiful.  相似文献