Transduction and Transplantation of Spermatogonia into the Testis of Ram Lambs through the Extra-testicular Rete     

JR Rodriguez-Sosa  JD Silvertown  RA Foster  JA Medin  A Hahnel 《Reproduction in domestic animals》2009,44(4):612-620

Spermatogonial transplantation will provide a new way to study spermatogenesis in domestic animals, disseminate male genetics and produce transgenic animals, if efficiency can be improved. We evaluated a 'surgical' method for transplanting donor cells into testes of ram lambs, where the head of the epididymis is reflected, and a catheter introduced into the extra-testicular rete testis. We also tested transduction of ram spermatogonia with a lentiviral (LV) vector as a means to identify permanent colonization, and introduce genes into donor cells. Eight ram lambs, 11- to 13-week olds, were the recipients: in five, spermatogonia were injected into one testis, and the contralateral testis was an un-manipulated control: in two, spermatogonia were injected into one testis and the contralateral was sham-injected: in one, both testes were injected. Six lambs received spermatogonia labelled with a cell-tracking dye and these were collected 1 or 2 weeks after transplantation; three lambs received spermatogonia transduced with a LV vector driving the expression of enhanced Green Fluorescence Protein and these were collected after 2 months. Donor cells were detected by immunohistochemistry in tubules of seven of nine recipient testes. Approximately 22% of tubule cross-sections contained donor cells immediately after transplantation, and 0.2% contained virally transduced cells 2 months after transplantation. The onset of spermatogenesis was delayed, and there were lesions in both injected and sham-injected testes. Despite the effects of the surgery, elongated spermatids were present in one recipient testis 2 months after surgery. The results suggest that, after modifying the surgical and transduction techniques, this approach will be a means to produce good colonization by donor spermatogonia in sheep testes.  相似文献   

Isolation of Canine Mammary Cells With Stem Cell Properties and Tumour-Initiating Potential     

C Cocola  P Anastasi  S Astigiano  E Piscitelli  P Pelucchi  L Vilardo  G Bertoli  M Beccaglia  MC Veronesi  S Sanzone  O. Barbieri  RA Reinbold  GC Luvoni  I Zucchi 《Reproduction in domestic animals》2009,44(S2):214-217

Recent data suggest that mammary carcinogenesis may be driven by cancer stem cells (CSCs) derived from mutated adult stem cells, which have acquired aberrant cell self-renewal or by progenitor cells that have acquired the capacity for cell self-renewal. Spontaneous mammary cancers in cats and dogs are important models for the understanding of human breast cancer and may represent alternative species model systems that can significantly contribute to the study of human oncogenesis. With the goal of identifying markers for isolating human breast CSCs, we have generated a canine model system to isolate and characterize normal and CSCs from dog mammary gland. Insight into the hierarchical organization of canine tumours may contribute to the development of universal concepts in oncogenesis by CSCs. Cells with stem cell properties were isolated from normal and tumoural canine breast tissue and propagated as mammospheres and tumourspheres in long-term non-adherent culture conditions. We showed that cells obtained from spheres that display self-renewing properties, have multi-lineage differentiation potential, could generate complex branched tubular structures in vitro and form tumours in NOD/SCID mice. We analysed these cells for the expression of human stem and CSC markers and are currently investigating the tumour-initiating properties of these cells and the hierarchical organization of normal and neoplastic canine mammary tissue.  相似文献   

Experimental studies of the role of the little raven (Corvus mellori) in surveillance for West Nile virus in Australia     

J Bingham  RA Lunt  DJ Green  KR Davies  V Stevens  FYK Wong 《Australian veterinary journal》2010,88(6):204-210

Objective To study the potential role of an Australian corvid, the little raven (Corvus mellori), in the surveillance for exotic West Nile virus (WNV) in Australia. Method In a series of trials, little ravens were infected with WNV (strain 4132 New York 1999) and Kunjin virus (strain K42886) by the intramuscular route. They were observed for 20 days during which blood and swab samples were taken for virus isolation. Tissue samples were taken from ravens humanely killed during the acute infection period, and at the termination of the trials, for virus isolation, histopathology and immunohistochemistry. Results Ravens infected with WNV became mildly ill, but all recovered and seroconverted. Blood virus titres peaked around 3 to 4 days after inoculation at levels between 10^3.0 to 10^7.5 plaque forming units/mL. Virus or viral antigen was detected in spleen, liver, lung, kidney, intestine, testis and ovary by virus isolation and/or immunohistochemistry. WNV was detected in oral and cloacal swabs from 2 to 7 days post inoculation. The molecular and pathogenic characteristics of the inocula were consistent with them being of high virulence, as expected for this isolate. Ravens infected with Kunjin virus developed viraemia and seroconverted, although they did not develop disease. Conclusions Little ravens do not develop severe disease in response to virulent WNV infection and for this reason may not be important sentinel hosts in the event of an outbreak of WNV, as in North America. However, as they have relatively high viraemias, they may be able to support virus cycles.  相似文献   

EFFECTS OF ANESTHETIC PROTOCOL ON NORMAL CANINE BRAIN UPTAKE OF 18F‐FDG ASSESSED BY PET/CT     

MIN SU LEE  JEFF KO  AH RA LEE  IN HYE LEE  MI AE JUNG  BRENDA AUSTIN  HYUNWOO CHUNG  SANGSOEP NAHM  KIDONG EOM 《Veterinary radiology & ultrasound》2010,51(2):130-135

The purpose of this study was to assess the effects of four anesthetic protocols on normal canine brain uptake of 2‐deoxy‐2‐[¹⁸F]fluoro‐d ‐glucose (FDG) using positron emission tomography/computed tomography (PET/CT). Five clinically normal beagle dogs were anesthetized with (1) propofol/isoflurane, (2) medetomidine/pentobarbital, (3) xylazine/ketamine, and (4) medetomidine/tiletamine–zolazepam in a randomized cross‐over design. The standard uptake value (SUV) of FDG was obtained in the frontal, parietal, temporal and occipital lobes, cerebellum, brainstem and whole brain, and compared within and between anesthetic protocols using the Friedman test with significance set at P<0.05. Significant differences in SUVs were observed in various part of the brain associated with each anesthetic protocol. The SUV for the frontal and occipital lobes was significantly higher than in the brainstem in all dogs. Dogs receiving medetomidine/tiletamine–zolazepam also had significantly higher whole brain SUVs than the propofol/isoflurane group. We concluded that each anesthetic protocol exerted a different regional brain glucose uptake pattern. As a result, when comparing brain glucose uptake using PET/CT, one should consider the effects of anesthetic protocols on different regions of the glucose uptake in the dog's brain.  相似文献   

IMAGING DIAGNOSIS—FDG‐PET/CT OF A CANINE SPLENIC PLASMA CELL TUMOR     

AH RA LEE  MIN SU LEE  IN SEONG JUNG  DAE YOUNG KIM  SANG SEOP NHAM  HYUN WOO CHUNG  KIDONG EOM 《Veterinary radiology & ultrasound》2010,51(2):145-147

An 8‐year‐old Shih Tzu developed abdominal pain and hyperglobulinemia. A round splenic mass was noted radiographically and sonographically. The patient was evaluated by fluorodeoxyglucose positron emission tomography coupled with computed tomography (FDG‐PET/CT). There was no evidence of metastasis or bone marrow involvement on PET/CT images. The standardized uptake value (SUV) of the splenic mass was increased over the reference range (SUV=4.83). The patient was diagnosed as splenic extramedullary plasmacytoma through immunohistopathologic study. After the splenectomy, the globulin level normalized and the patient is alive without complications.  相似文献   

Field scale spatiotemporal analysis of surface soil moisture for evaluating point-scale in situ networks     

Gary C. Heathman  Michael H. Cosh  Eunjin Han  Thomas J. Jackson  Lynn McKee  Scott McAfee 《Geoderma》2012

Soil moisture is an intrinsic state variable that varies considerably in space and time. From a hydrologic viewpoint, soil moisture controls runoff, infiltration, storage and drainage. Soil moisture determines the partitioning of the incoming radiation between latent and sensible heat fluxes. Although soil moisture may be highly variable in space and time, if measurements of soil moisture at the field or small watershed scale are repeatedly observed, certain locations can often be identified as being temporally stable and representative of the an area average. This study is aimed at determining the adequacy of long term point-scale surface soil moisture measurements in representing local field scale averages which may ultimately serve as in situ locations for the calibration and validation of remotely sensed soil moisture. Experimental data were obtained by frequency-domain reflectometry (FDR) sensors permanently installed in two agricultural fields, AS1 and AS2 (2.23 and 2.71 ha, respectively) at a depth of 5 cm. Twenty additional FDR sensors, spaced 35 m apart, were installed horizontally at a depth of 5 cm in each field with automated data collection being transmitted every 30 min from July 15 through September 20, 2009. Additionally, meteorological data were obtained from existing weather stations in each field. The FDR sensors revealed persistent patterns in surface soil moisture within each field and identified sites that were temporally stable. The locations that were optimal for estimating the area-average field water contents were different from the permanent sensor locations in both fields. Permanent sensor data showed approximately 4 and 10% mean relative differences for fields AS1 and AS2, respectively, with relatively large standard deviations. Thus, minimum offset values could be applied to the temporally stable field sites to obtain representative field average values of surface soil moisture. However, use of permanent sensor data for offset estimates gave poor results. These findings are of relevance for applications of geospatial surface soil moisture data assimilation in hydrologic modeling when only point-scale observations are available, as well as, remotely sensed surface soil moisture calibration and validation studies.  相似文献   

Deleterious Effects of Progestagen Treatment in VEGF Expression in Corpora Lutea of Pregnant Ewes     

CA Letelier  MA Sanchez  RA Garcia‐Fernandez  B Sanchez  P Garcia‐Palencia  A Gonzalez‐Bulnes  JM Flores 《Reproduction in domestic animals》2011,46(3):481-488

The aim of the current study was to determine the possible effects of progestagen oestrous synchronization on vascular endothelial growth factor (VEGF) expression during sheep luteogenesis and the peri‐implantation period and the relationship with luteal function. At days 9, 11, 13, 15, 17 and 21 of pregnancy, the ovaries from 30 progestagen treated and 30 ewes cycling after cloprostenol injection were evaluated by ultrasonography and, thereafter, collected and processed for immunohistochemical evaluation of VEGF; blood samples were drawn for evaluating plasma progesterone. The progestagen‐treated group showed smaller corpora lutea than cloprostenol‐treated and lower progesterone secretion. The expression of VEGF in the luteal cells increased with time in the cloprostenol group, but not in the progestagen‐treated group, which even showed a decrease between days 11 and 13. In progestagen‐treated sheep, VEGF expression in granulosa‐derived parenchymal lobule capillaries was correlated with the size of the luteal tissue, larger corpora lutea had higher expression, and tended to have a higher progesterone secretion. In conclusion, the current study indicates the existence of deleterious effects from exogenous progestagen treatments on progesterone secretion from induced corpora lutea, which correlate with alterations in the expression of VEGF in the luteal tissue and, this, presumably in the processes of neoangiogenesis and luteogenesis.  相似文献   

Tick paralysis in dogs and cats in Australia: treatment and prevention deliverables from 100 years of research     

AM Padula  EM Leister  RA Webster 《Australian veterinary journal》2020,98(1-2):53-59

This review of tick paralysis caused by Ixodes holocyclus in Australia addresses the question: What are the key discoveries that have enabled effective treatment and prevention of tick paralysis in dogs and cats? Critical examination of 100 years of literature reveals that arguably only three achievements have advanced treatment and prevention of tick paralysis in animals. First, the most significant treatment advance was the commercial availability of tick antiserum in the 1930s. Hyperimmune serum currently remains the only specific anti-paralysis tick therapy available to veterinarians in Australia. Second, advances in veterinary critical care have increased survival rates of the most severely affected dogs and cats. Critical care advancements have been enabled through specialised veterinary hospitals that can provide appropriate care 24 h a day, and advanced training of veterinarians, veterinary nurses and technicians. Third, perhaps that biggest advance of all in the last 100 years of research has been the commercial availability of the isooxazoline class of acaricidal preventatives in Australia specifically for I. holocyclus. This highly effective class of preventatives offers long duration of action, low cost, spot-on or oral formulations and a low rate of adverse reactions. Animal owners and veterinarians now have the most useful tool of all – a reliable preventative. This review reveals the key events in research over the last 100 years and the tortuous pathway to delivering better treatment and preventative options for this enigmatic Australian parasite.  相似文献   

Estimation of wildlife damage from federal crop insurance data     

Sophie C McKee  Stephanie A Shwiff  Aaron M Anderson 《Pest management science》2021,77(1):406-416

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Quality evaluation of commercial chile-based salsas     

McKee  L.H.  Christopher  J.  Bock  M.A. 《Plant foods for human nutrition (Dordrecht, Netherlands)》2003,58(3):1-12

A standardized extrusion cooking process was developed for production of a high protein weaning food based on peanuts, maize and soybeans. Major factors evaluated included the effects of blend formulation, extrusion temperature and feed moisture content on ease of extrusion and product quality characteristics. Results showed bulk density and hardness increased while expansion index decreased with increase in feed moisture content. At a fixed range of feed moisture content, product bulk density and firmness decreased while expansion index increased with increasing extrusion temperature. For ease of extrusion and best product quality in terms of sensory attributes and cooking properties, the following extrusion parameters were established for a blend formulation of 75% maize, 10% peanut and 15% soybean: feed particle size of 300–400 m extruded using a screw speed of 500 rpm, with a feed rate of 4.6 kg/min, feed moisture content of 16–18%, and extrusion temperature of 100 ,°C–105,°C. Pair-wise comparison of the sensory attributes of porridges prepared from milled samples of the weaning foods showed significant differences between extruded products and existing traditional counterparts, with very high scores for all sensory attributes of the extruded products, especially extruded raw (non-roasted) blend samples. In the Home-Use-Test, at least 92% of respondents in two out of the three major ecological zones of Ghana placed overall sensory and functional characteristics of extruded raw blend samples as highly acceptable. About 7% of respondents scored sensory and functional quality attributes as acceptable.  相似文献