Fatal post-anaesthetic pneumothorax in a dog     

L Harvey  PJ Murison  D Fews  & JC Murrell 《Veterinary anaesthesia and analgesia》2010,37(1):83-84

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A quantitative real‐time PCR assay for detection of Colletotrichum lindemuthianum in navy bean seeds     

Y. Y. Chen  R. L. Conner  C. L. Gillard  D. L. McLaren  G. J. Boland  P. M. Balasubramanian  C. Stasolla  Q. X. Zhou  S. F. Hwang  K. F. Chang  C. Babcock 《Plant pathology》2013,62(4):900-907

Bean anthracnose is a seedborne disease of common bean (Phaseolus vulgaris) caused by the fungal pathogen Colletotrichum lindemuthianum. Using seed that did not test positive for the pathogen has been proven to be an effective strategy for bean anthracnose control. To quantify the extent of anthracnose seed infection, a real‐time PCR‐based diagnostic assay was developed for detecting C. lindemuthianum in seeds of the commercial bean class navy bean. The ribosomal DNA (rDNA) region consisting of part of the18S rDNA, 5^.8S rDNA, internal transcribed spacers (ITS) 1, 2 and part of the 28S rDNA of seven races of C. lindemuthianum, 21 isolates of Colletotrichum species and nine other bean pathogens were sequenced with the universal primer set ITS5/ITS4. Based on the aligned sequence matrix, one primer set and a probe were designed for a SYBR Green dye assay and a TaqMan MGB (minor groove binder) assay. The primer set was demonstrated to be specific for C. lindemuthianum and showed a high sensitivity for the target pathogen. The detection limit of both assays was 5 fg of C. lindemuthianum genomic DNA. To explore the correlation between the lesion area and the DNA amount of C. lindemuthianum in bean seed, seeds of the navy bean cultivar Navigator with lesions of different sizes, as well as symptomless seeds, were used in both real‐time PCR assays.  相似文献   

A biotyping scheme for Salmonella livingstone     

M O Odongo  I M McLaren  J E Smith  C Wray 《The British veterinary journal》1990,146(1):75-79

Salmonella livingstone is one of the more common salmonella serotypes isolated in the United Kingdom. The characterization of 70 different isolates of S. livingstone using biochemical tests and plasmid profile analysis is described. The isolates could be divided into four groups by their ability to grow in d-tartrate, l-tartrate and Stern's glycerol. Further subdivision was achieved by the use of plasmid profile analysis; 24 of the isolates possessed light plasmids (less than 9.5 MD) and four possessed heavy plasmids (greater than 30 MD). The combination of biotyping and plasmid profile analysis can be used as the basis of a scheme to 'fingerprint' S. livingstone for epidemiological studies.  相似文献   

Adsorption and activity of chitinase on kaolinite     

J. Skujiņš  A. Puķite  A.D. McLaren 《Soil biology & biochemistry》1974,6(3):179-182

Chitinase is adsorbed on kaolinite below the isoelectric point of the enzyme, pH 6.8. Maximum adsorption is reached below pH 4.6. The amount of chitinase adsorbed to kaolinite depends on the concentration and on the ratio of enzyme to kaolinite. Adsorption results in reduction of chitinase activity. the extent of which depends on the amount of kaolinite present, on pH, and on the length of exposure time. Upon sorption of chitinase on kaolinite the optimal pH for activity is increased from pH 4.7 to 5.7.  相似文献   

Heterozygote detection for maple syrup urine disease in cattle     

PJ HEALY  JA DENNIS 《Australian veterinary journal》1995,72(9):346-348

SUMMARY The clinical, pathological and biochemical manifestations of maple syrup urine disease (MSUD) are similar in Poll Hereford and Poll Shorthorn X Poll Hereford calves. No significant differences were observed in branched-chain amino acid concentrations in plasma, or of branched-chain keto acid dehydrogenase activity in fibroblasts, between Poll Herefords homozygous normal and heterozygous for the mutation responsible for MSUD. Haemopoietic chimerism resulted in incorrect diagnosis of the MSUD genotype in 30% of non-identical twins when blood DNA was analysed using allele-specific amplification. Hair roots are shown to be a suitable source of target DNA for genotyping Poll Hereford cattle for the MSUD mutation. Twelve of 203 (5.8%) aged Poll Hereford bulls, sampled at saleyards during the last 4 months of 1993, were found to be heterozygous for the mutation. In contrast, the mutant sequence was detected in only 1 of 150 (0.7%) 2- and 3-year-old Poll Hereford bulls offered for sale at 2 stud sales held during 1993, suggesting that the prevalence of the disease may decline over the next few years.  相似文献   

Regulation of Prostaglandin Secretion and Proliferation of Bovine Endometrial Stromal Cells by Platelet-activating Factor     

U Tiemann  JA Davidson  PJ Hansen 《Reproduction in domestic animals》1995,30(3):141-143

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Dose response in growth of pigs injected daily with porcine somatotropin from 57 to 103 kilograms     

D G McLaren  P J Bechtel  G L Grebner  J Novakofski  F K McKeith  R W Jones  R H Dalrymple  R A Easter 《Journal of animal science》1990,68(3):640-651

Exogenous administration of porcine somatotropin (PST) has been shown to promote growth in the pig; however, dose-response relationships and interactions with PST source and sex for animals taken to market weight have not been established clearly. The present study was conducted to determine the relationship between dosage of pituitary-derived and recombinantly manufactured PST (pPST and rPST) and growth (ADG), structural soundness, gain-to-feed ratio (G/F) and average daily feed intake (ADF). Crossbred barrows (n = 113) and gilts (n = 97) were injected with either saline, 1.5, 3.0, 6.0 or 9.0 mg/d of pPST or rPST from 57 +/- .3 to 103.5 +/- .7 kg live weight. Pigs were housed five per pen and had ad libitum access to an 18% crude protein diet for the duration of the experiment. Response curves for pPST and rPST did not differ (P greater than .20) for ADG, soundness score or ADF. Although regression coefficients for response of G/F to pPST and rPST differed (P = .05), pairwise comparisons of treatment means did not (P greater than .10). Response curves for barrows and gilts did not differ for ADG or soundness (P greater than .05). Averaged over PST source and sex, quadratic dose-responses were detected for ADG, G/F and ADF (P less than .01), but PST had no effect on soundness (P greater than .25). Exponential regression models best described the dose-response relationships, and 6.0 mg.pig-1.d-1 was predicted to result in 95% of maximal achievable response for days to 103.5 kg and G/F. At this dose, pigs were predicted to grow .15 kg/d (20%) faster during the treatment period, reach slaughter weight 11.6 d earlier, consume .56 kg/d (19%) less feed, and have a G/F .095 (36%) greater than controls.  相似文献   

βbT-mannosidosis in Salers calves in Australia     

PJ HEALY  GN KIDD  RE REUTE  C BUNCE  I HOSIE  T STAPLETON 《Australian veterinary journal》1992,69(6):145-145

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The comparative pathogenicity of four serovars of Actinobacillus (Haemophilus) pleuropneumoniae   总被引：1，自引：0，他引：1  

RJ ROGERS  LE EAVES  PJ BLACKALL  KF TRUMAN 《Australian veterinary journal》1990,67(1):9-12

The pathogenicity of 2 isolates of each of serovars 7, 3, 1 and 2 of Actinobacillus pleuropneumoniae was tested by intranasal inoculation into 60, 6-week-old large white pigs. Four dose rates varying from 0.27 to 560 x 10(6) organisms per pig with 10-fold serial dilutions were used. Surviving pigs were necropsied 7 days after inoculation. The proportion of pigs dying and developing gross lesions following infection was significantly greater for pigs given serotype 1 than for each of the other 3 serotypes, which did not differ significantly from each other. Twelve of 16 pigs given either of the 2 isolates of serovar 1 died after acute illness and 1 of 44 pigs given either of the 2 isolates each of serovars 7, 3 and 2 died. Pigs given serovar 1 showed high temperatures, severe respiratory distress, frothy haemorrhagic nasal discharge and weight loss. Lung lesions were produced in all 16 pigs given serovar 1, in 7 of 14 pigs given serovar 7, 7 of 14 pigs receiving serovar 3 and in 5 of 16 pigs given serovar 2. The lethal infections were characterised by a severe acute fibrinohaemorrhagic necrotising pleuropneumonia, whereas non-lethal cases had lung lesions ranging from necrotising purulent pleuropneumonia to abscessation. Significant differences between isolates in proportions of tissues culture positive for A. pleuropneumoniae for serovars 7 and 2, but not for serovars 3 and 1 suggested that isolates may vary in virulence within serovars, but more detailed studies are needed to clarify this point.  相似文献   

Cerebellar abiotrophy in Angus cattle     

PJ MITCHELL  W. REILLY  PAW HARPER  CJ McCAUGHAN 《Australian veterinary journal》1993,70(2):67-68

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