Molecular epidemiology of clinical isolates of Pseudomonas aeruginosa isolated from horses in Ireland     

Tazumi A  Maeda Y  Buckley T  Millar B  Goldsmith C  Dooley J  Elborn J  Matsuda M  Moore J 《Irish veterinary journal》2009,62(7):456-459

Clinical isolates (n = 63) of Pseudomonas aeruginosa obtained from various sites in 63 horses were compared using ERIC2 RAPD PCR to determine their genetic relatedness. Resulting banding patterns (n = 24 genotypes) showed a high degree of genetic heterogeneity amongst all isolates examined, indicating a relative non-clonal relationship between isolates from these patients, employing this genotyping technique. This study characterised 63 clinical isolates into 24 distinct genotypes, with the largest cluster (genotype E) accounting for 10/63 (15.9%) of the isolates. ERIC2 RAPD PCR proved to be a highly discriminatory molecular typing tool of P. aeruginosa in isolates recovered from horses. With the adoption of several controls to aid reproducibility, this technique may be useful as an alternative to PFGE, particularly in epidemiological investigations of outbreaks where speed may be a significant parameter. This is the first report of clonal heterogeneity amongst P. aeruginosa from horses and demonstrated that ERIC RAPD PCR is a rapid method for the examination of this species in horses, which may be useful in outbreak analysis.  相似文献   

Serological survey of pre-weaned New Zealand fur seals (Arctocephalus forsteri) for brucellosis and leptospirosis     

GF Mackereth  KM Webb  JS O'Keefe  PJ Duignan  R Kittelberger 《New Zealand veterinary journal》2013,61(6):428-432

AIM: To conduct a longitudinal serological survey for evidence of Brucella spp and Leptospira spp infection of pre-weaned New Zealand fur seals in a colony on the Otago Peninsula.

METHODS: Seal pups were repeatedly captured on a monthly basis from February through to July 2001. Pups were tagged at first capture and a blood sample was taken at each capture event. A total of 163 sera were collected from 118 seal pups. Where sufficient volume was collected, the sera were tested for leptospirosis using the microscopic agglutination test (MAT), and for brucellosis using the competitive enzyme-linked immunosorbent assay (ELISA) for Brucella abortus.

RESULTS: None of 128 sera from 101 seals tested positive to the ELISA for B. abortus. All tests for Leptospira interrogans serovars Grippotyphosa, Copenhageni, Bratislava and Leptospira borgpetersenii serovar Ballum were negative at a cut-off of <1/100 dilution. Positive or suspicious titres were found to L. interrogans serovars Canicola and Pomona and L. borgpetersenii serovar Hardjo. The highest titres (12,800) were found to serovar Pomona. The titre to serovar Pomona in one seal rose from <1/50 in March to 12,800 in April and was <1/50 when re-sampled in July. The titre to serovar Pomona in another seal dropped from 12,800 in May to <1/50 in June. These seals also had titres to serovar Hardjo, which rose or fell in the same manner. All suspicious or positive titres occurred in late April and early May, when the pups were approximately 4–5 months old. In June and July, all seals tested were negative.

CONCLUSIONS: There was no serological evidence of Brucella infection in the pre-weaned fur seals at the colony. Positive titres to serovars Pomona, Hardjo, or Canicola suggest that a Leptospira species was present at the colony, however isolation or visualisation of the organism is required to confirm this. Care should be exercised when handling New Zealand fur seals to prevent human infection or inadvertent transfer of leptospirosis to another marine mammal species.  相似文献   

Direct Radiative Forcing by Smoke from Biomass Burning     

PV Hobbs  JS Reid  RA Kotchenruther  RJ Ferek  R Weiss 《Science (New York, N.Y.)》1997,275(5307):1776-1778

Airborne measurements in smoke from biomass burning in Brazil have yielded optical parameters that permit an improved assessment of the effects of smoke on Earth's radiation balance. The global-mean direct radiative forcing due to smoke from biomass burning worldwide is estimated to be no more than about -0.3 watt per square meter (cooling), compared with +2.45 watts per square meter (warming) due to anthropogenic greenhouse gases. On regional scales, direct radiative forcing due to smoke can be large and might indirectly affect global climate.  相似文献   

Retinal detachment postphacoemulsification in Bichon Frises: a retrospective study of 54 dogs          下载免费PDF全文

Silvia G. Pryor  Ellison Bentley  Gillian J. McLellan  Elizabeth A. Giuliano  Rachel A. Allbaugh  Amy J. Rankin  Amber L. Labelle  Kevin A. Buhr 《Veterinary ophthalmology》2016,19(5):373-378

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Science‐in‐brief: Bisphosphonate use in the racehorse: Safe or unsafe?          下载免费PDF全文

J. McLellan 《Equine veterinary journal》2017,49(4):404-407

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Earth-Based Radio Tracking of the Galileo Probe for Jupiter Wind Estimation     

WM Folkner  RA Preston  JS Border  J Navarro  WE Wilson  M Oestreich 《Science (New York, N.Y.)》1997,275(5300):644-646

Although the Galileo probe was designed to communicate only to the orbiter, the probe radio signal was detected at two Earth-based radio observatories where the signal was a billion times weaker. The measured signal frequency was used to derive a vertical profile of the jovian zonal wind speed. Due to the mission geometry, the Earth-based wind estimates are less sensitive to descent trajectory errors than estimates based on probe-orbiter Doppler measurements. The two estimates of wind profiles agree qualitatively; both show high wind speeds at all depths sampled.  相似文献   

猪舍内气流变化的模拟研究   总被引：6，自引：0，他引：6  

佟国红  张国强  MORSING S  STROM JS  RAVN P 《沈阳农业大学学报》2007,38(3):379-382

圈养动物舍内的气流变化对于室内的污染物扩散起到非常关键的作用,采用基于有限容积方法的计算流体动力学(CFD)软件对建于奥胡斯大学农业工程研究所空气物理实验室中的1:12.5猪舍模型内的气流进行了稳态和三维模拟。将模拟的气流速度与室内一个参考点的实际测试速度进行了对比,同时分析了气流入口不同紊流动能及扩散率取值对室内测试参考点气流速度模拟值的影响。结果表明,模拟值与测试值的相对误差在0.4%～11.4%之间。  相似文献   

Ecology. North Atlantic right whales in crisis     

Kraus SD  Brown MW  Caswell H  Clark CW  Fujiwara M  Hamilton PK  Kenney RD  Knowlton AR  Landry S  Mayo CA  McLellan WA  Moore MJ  Nowacek DP  Pabst DA  Read AJ  Rolland RM 《Science (New York, N.Y.)》2005,309(5734):561-562

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Does periosteal scraping of the third metacarpal bone reduce the incidence of ‘bucked shins’ in young Thoroughbred racehorses?     

S. Plevin  J. McLellan 《Equine veterinary journal》2014,46(5):560-566

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Injury of the origin of the gastrocnemius and superficial digital flexor muscles in a Thoroughbred racehorse: An atypical presentation of reciprocal apparatus failure     

J. McLellan  J.‐M. Denoix 《Equine Veterinary Education》2014,26(8):402-406

Damage to the origin of the gastrocnemius and superficial digital flexor muscles has been previously reported as an acute injury in both foals and adult horses. This case report describes, for the first time, the clinical onset of caudal reciprocal apparatus failure in a 2‐year‐old Thoroughbred. These signs were due to injury of the gastrocnemius and superficial digital flexor muscle origins sustained prior to the onset of lameness. This condition should be considered in cases demonstrating similar clinical signs, even in the absence of known trauma or acute injury to the region.  相似文献