Genotype × environment interactions in reproductive traits of Nellore cattle in northeastern Brazil     

Diego Pagung Ambrosini  Carlos Henrique Mendes Malhado  Raimundo Martins Filho  Fernando Flores Cardoso  Paulo Luiz Souza Carneiro 《Tropical animal health and production》2016,48(7):1401-1407

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Lectin histochemical pattern on the normal and cystic ovaries of sows     

Marcelo HAF Mendes  Maria HB Pinto  Eduardo J Gimeno  Claudio G Barbeito  Fabiano JF de Sant'Ana 《Reproduction in domestic animals》2019,54(10):1366-1374

The lectin histochemical pattern (LHP) was characterized and compared in normal and cystic ovaries of sows. Six biotinylated lectins (PNA, SBA, WGA, RCA‐1, DBA and UEA‐1) were used on tissue sections. In the normal ovaries, the reaction to UEA‐1 and SBA was mild to moderate in mesothelial and endothelial cells. RCA‐1 staining was mild to moderate in theca interna of growing follicles, corpora luteum and mesothelium. In addition, this lectin presented strong reaction in endothelial cells, granulosa cells of atretic follicles, zona pellucida of growing follicles and plasma. DBA showed strong intensity in mesothelial and endothelial cells. There was mild to moderate reactivity to WGA in granulosa cells, corpus luteum and theca interna of follicles in development, and moderate in zona pellucida, in granulosa cells of atretic follicles and mesothelium. PNA staining was mild to moderate in oocytes and in the adventitia and media of medullary arteries. Changes in the LHP of the cystic ovaries were noted; however, there were no differences in these findings between the follicular and luteinized cysts. UEA‐1 reactivity in the cystic ovaries was moderately reduced in the mesothelial and endothelial cells, whereas there was mild reduction in the DBA staining in the granulosa cells. Reaction to RCA‐1 and WGA in the cysts also was decreased in theca interna, zona pellucida and granulosa cells of atretic follicles. Furthermore, endothelium and theca interna in the cystic ovaries presented mild reduction of marcation to SBA, whereas there was decreased reactivity to PNA in the oocytes and adventitia and media layers of the medullary arteries. The results of the current study show that cysts modify the LHP in swine ovaries. These changes of glycoconjugates in many ovarian structures could modify diverse process and may be one of the reasons for decreased fertility in sows.  相似文献   

Activity of Hypericum brasiliense and Hypericum cordatum on the central nervous system in rodents     

Rieli Mendes F  Mattei R  de Araújo Carlini EL 《Fitoterapia》2002,73(6):462-471

Hydroalcoholic extracts from species Hypericum brasiliense Choisy (HB) and Hypericum cordatum (Vell. Conc.) N. Robson (HC), were evaluated on the central nervous system (CNS) in some pharmacological tests. Signs of toxicity were observed for both species during the initial screening when high doses of up to 100 mg/kg (i.p.) and 1000 mg/kg (oral) were utilized. HC presented greater toxicity, with LD(50) of 269 mg/kg, as compared to HB (537 mg/kg). Alterations in sleeping time and in motor coordination were not observed both for HB and for HC. On the other hand, both species showed signs of general depressant action on the CNS, verified by decreased motor activity. Furthermore, animals treated with HB presented an increase in response time to thermal stimulus with doses of 50 mg/kg (i.p.) and 500 mg/kg (oral) suggesting possible analgesic action. Both HB and HC were tested in animal models to verify antidepressant action (forced swimming and hypothermy induced by apomorphine). In these tests, neither of the plants inhibited hypothermy, nor did they reduce immobility time in forced swimming.  相似文献   

Humoral immune response in goats immunised with cathepsin L1, peroxiredoxin and Sm14 antigen and experimentally challenged with Fasciola hepatica     

Buffoni L  Martínez-Moreno FJ  Zafra R  Mendes RE  Pérez-Écija A  Sekiya M  Mulcahy G  Pérez J  Martínez-Moreno A 《Veterinary parasitology》2012,185(2-4):315-321

The humoral immune response was analysed in goats immunised with FhCL1, FhPrx, Sm14, and experimentally challenged with Fasciola hepatica. All immunised animals developed significant levels of anti-fluke specific antibodies and those immunised with FhCL1 showed the highest antibody titre. After experimental infection, an increase in the antibody level was detected only in goats immunised with FhCL1. In the adjuvant-control animals, the experimental challenge induced significant production of specific antibodies against FhCL1, FhPrx and Sm14. While liver fluke specific humoral responses were seen in all groups, no significant protection in any of the vaccinated groups was found.  相似文献   

Treatment of Waters Containing the Thiocarbamate Herbicide Molinate through an Adsorption/Bio-Regeneration System using a Low-Cost Adsorbent     

Débora Carvalho  Adélio Mendes  Fernão D. Magalhães  Olga C. Nunes 《Water, air, and soil pollution》2010,207(1-4):289-298

The feasibility of using recycled granular tire rubber (GTR) to remove molinate from contaminated water bodies was evaluated in this study. Adsorption equilibrium data was well described by a linear isotherm, and the adsorption was completely reversible. Breakthrough curves showed column efficiencies of approximately 40%, based on total capacity, and complete bed regeneration was achieved using clean water. The effluent from the regeneration step was successfully decontaminated using a defined bacterial mixed culture, capable of molinate mineralization. It was shown that this treated water can be used for regenerating a subsequently saturated bed. The GTR adsorbent showed two important features: complete reversibility towards molinate adsorption and stability along successive adsorption/bio-regeneration cycles. Common adsorbents, such as activated carbons and resins, loose performance very quickly under the same conditions, due to irreversible adsorption.  相似文献   

Technical note: Evaluation of bimodal distribution models to determine meal criterion in heifers fed a high-grain diet     

Bailey JC  Tedeschi LO  M Mendes ED  Sawyer JE  Carstens GE 《Journal of animal science》2012,90(8):2750-2753

Meals are clusters of feedbunk visit (BV) events that are differentiated from the next meal by a nonfeeding interval that is longer compared with the nonfeeding intervals within a meal. The longest nonfeeding interval considered to be part of a meal is defined as the meal criterion. The objective of this study was to determine which combination of 2 probability density functions [(PDF): Gaussian normal (G), Weibull (W), Log-Normal, Gamma, and Gumbel] used in a bimodal distribution model had the best fit of nonfeeding interval data collected in beef heifers. Feeding behavior traits (572,627 total BV events) were measured in 119 heifers fed a high-grain diet (3.08 Mcal ME/kg DM), using a GrowSafe system for 66 d. The frequency and duration of BV events averaged 75 ± 15 events/d and 73.0 ± 22.3 min/d, respectively. The bimodal PDF combinations were fitted to the log(10)-transformed interval lengths between BV events for each animal, using R mixdist package (2.13). The Akaike Information Criterion (AIC) was used to assess goodness of fit of the 25 bimodal PDF combinations. The PDF model with the least AIC value was selected as the best fit for each individual. A χ(2) analysis of the selected best PDF distribution across individuals revealed that 78.2% of the heifers best fit were G-W or W-W PDF models. The likelihood probability estimates were calculated from the average AIC deviation of each model from the standard G-G model. The G-W likelihood probability estimate was greater (P = 0.001) than the W-W combination (0.997 vs. 0.727). Our analysis indicated the G-W model had a statistically better fit and is most likely the best approach to define meal criterion in beef heifers fed high-grain diets.  相似文献   

Effect of age and abomasal puncture on peritoneal fluid, hematology, and serum biochemical analyses in young calves     

Mendes LC  Peiró JR  Feitosa FL  Luvizotto MC  Borges AS  Ciarlini PC  Perri SH 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2005,19(6):899-904

The goals of this study were to evaluate techniques for collection of peritoneal fluid from calves, establish reference ranges for fibrinogen in peritoneal fluid during the 1st month of life, and determine if abomasal puncture would alter peritoneal fluid or hematologic variables. Twenty-two healthy Holstein calves underwent 3 peritoneal fluid collections on day 1, day 15, and day 30 of age. Fibrinogen concentration in peritoneal fluid was 0.20 g/dL and 0.10 g/dL (P < .05) for day 1 and day 30, respectively, and 0.10 at day 15 (P > .05) for calves without abomasal puncture. Plasma fibrinogen concentration was 0.60 g/dL and 0.70 g/ dL (P < .05) for days 15 and 30, respectively, in calves without abomasal puncture. There were no significant differences (P < or = .05) in peritoneal fluid and peripheral blood total protein and fibrinogen concentrations, specific gravity, total and differential cell count, or erythrocyte counts between calves with or without abomasal puncture. We concluded that the reference ranges established for fibrinogen and total protein concentration are important for accurate evaluation of peritoneal fluid in calves for further comparison with similar-aged animals with gastrointestinal-tract or abdominal-cavity disease. Additionally, accidental abomasal puncture does not alter values of fibrinogen, total protein, and nucleated cell count in peritoneal fluid and does not cause apparent clinical abnormalities.  相似文献   

Myelopathy in Holstein x Gir calves in Brazil     

Borges AS  Mendes LC  Luvizotto MC  Summers B  de Lahunta A 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(5):730-731

This case report contains clinical and pathologic features of a degenerative myelopathy in Holstein X Gir crossbred calves in Brazil. The bilateral and symmetrical spinal cord white matter lesions were interpreted as a primary axonopathy that may be of the dying-back type.  相似文献   

Preliminary Trial: Motility Comparisons of a Unique Freezing Technology (UFT) to Liquid Nitrogen Mist Methodology for Cryopreservation of Porcine Spermatozoa     

HA Goolsby  JR Blanton  PZ Cotter  SD Prien 《Reproduction in domestic animals》2004,39(5):328-332

The motility outcomes of boar semen frozen with newly developed freezing techniques using a new unique freezing technology (UFT) compared with traditional liquid nitrogen methodology were investigated with the intent of improving current fertility outcomes using semen. The UFT is an electronically controlled cooling chamber that houses an organic fluid bath that can be maintained at temperatures below 0 degrees C without solidifying to freeze samples. Four ejaculates from four different boars were collected for this trial. Samples were handled consistently during the pre- and post-freeze processing. From each ejaculate, samples were separated into eight cryopreservation treatment groups, six UFT variations and two control liquid nitrogen groups, immediately before freezing, in replicates of two. After the initial cryopreservation was complete, all samples were stored in liquid nitrogen for at least 48 h. Post-thaw motilities and original motility return percentages were assessed on a random, individual-sample basis. After the initial evaluations, samples from two boars were recollected and frozen using the UFT for breeding purposes. Four sows were bred with the UFT frozen semen to confirm fertility capability. When assessing the individual UFT techniques, all of six UFT techniques had improved post-thaw motilities. However, treatments F (micro = 29%, return micro = 37%) and J (micro = 27%, return micro = 34%) showed the highest statistical improvement for post-thaw (p < 0.05) and original motility percent returns (p < 0.05) when compared with either the control cryo-tube (micro = 15%, return micro = 19%) or straw groups (micro = 12%, return micro = 16%). The UFT semen had a 50% conception rate, with an average of seven piglets from the sows that farrowed. Our preliminary data suggest a higher motility return with a slower pre-freeze phase below the freezing point before the acceleration to liquid nitrogen temperatures. The preliminary data suggest that the UFT could be utilized as a potential cryopreservation option for boar semen.  相似文献   

Evaluation of dietary histidine supplementation on meagre,Argyrosomus regius,juvenile growth,haematological profile,stress and muscle cellularity     

Margarida Saavedra  Teresa G. Pereira  Laura Ribeiro  Marisa Barata  Ana Candeias‐Mendes  Luis E. C. Conceio  Pedro Pouso‐Ferreira 《Aquaculture Nutrition》2020,26(4):1223-1230

Histidine plays an important role in haemoglobin synthesis, antioxidant defence system and stress resistance, and it is thought to be a limiting amino acid in most meagre diets. In this study, two different diets, a control and a diet supplemented with 3% histidine, were tested in 60‐day‐old meagre (initial weight—6.2 g) for 82 days. Survival varied between 92% and 94%, and neither SGR nor whole fish composition seemed to be affected by the diet. When fish were submitted to a stress test (outside water for 30 s), it was observed that the levels of glucose and lactate significantly increased in both treatments. The concentration of cortisol increased after the stress test only in the His 30 g/kg group. Muscle cellularity was affected by the diets, where the control group showed a higher mean fibre area and lower fibre density. These results, associated with a lower frequency of smaller fibres, suggest a decrease in fibre recruitment and a higher fibre hypertrophy. In conclusion, the supplementation of diets with 3% led to a more pronounced response to the stress test in terms of cortisol levels and increased muscle fibre recruitment, decreasing muscle fibre hypertrophy contribution to muscle growth.  相似文献