奶牛隐性子宫内膜炎诊断技术研究进展     

闫宝琪  董书伟  王东升  那立冬  杨洪早  张世栋  严作廷 《中国畜牧兽医》2016,43(3):683-688

奶牛隐性子宫内膜炎在以子宫内膜被嗜中性粒细胞(PMN)持续性的广泛浸润为主要特征,没有直观的临床症状,经常导致奶牛屡配不孕。在中国,由子宫内膜炎引起的奶牛不孕的比例达50.62%,且由隐性子宫内膜炎引起的奶牛不孕占其中的90%。由于隐性子宫内膜炎在发病初期不易被观察到,容易错过最佳治疗时间,造成巨大的经济损失,故如何对其做出正确、及时的诊断成了近年来的研究热点。目前,国内外奶牛隐性子宫内膜炎的诊断方法主要有阴道内窥镜法、细胞刷法、活体组织诊断法、超声诊断法及白血球酯酶法等,但目前这些诊断方法没有统一的判断标准。本文对几种不同的奶牛隐性子宫内膜炎的诊断方法进行了综述,为更早、更准确地诊断隐性子宫内膜炎提供依据。  相似文献   

Validation of an ELISA method for the serological diagnosis of canine brucellosis due to Brucella canis     

Maria Zoraida Daltro de Oliveira  Vera Vale  Lara Keid  Songeli Menezes Freire  Roberto Meyer  Ricardo Wagner Portela  Stella Maria Barrouin-Melo   《Research in veterinary science》2011,90(3):425-431

In the present study, the validation of an enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of canine brucellosis is described. Two different antigenic extracts, obtained by heat or ultrasonic homogenization of microbial antigens from a wild isolate of Brucella canis bacteria, were compared by ELISA and Western blot (WB). A total of 145 canine sera were used to define sensitivity, specificity and accuracy of the ELISA as follows: (1) sera from 34 animals with natural B. canis infection, confirmed by blood culture and PCR, as well as 51 sera samples from healthy dogs with negative results by the agar–gel immunodiffusion (AGID) test for canine brucellosis, were used as the control panel for B. canis infection; and (2) to scrutinize the possibility of cross reactions with other common dog infections in the same geographical area in Brazil, 60 sera samples from dogs harboring known infections by Leptospira sp., Ehrlichia canis, canine distemper virus (CDV), Neospora caninum, Babesia canis and Leishmania chagasi (10 in each group) were included in the study. The ELISA using heat soluble bacterial extract (HE-antigen) as antigen showed the best values of sensitivity (91.18%), specificity (100%) and accuracy (96.47%). In the WB analyses, the HE-antigen showed no cross-reactivity with sera from dogs with different infections, while the B. canis sonicate had various protein bands identified by those sera. The performance of the ELISA standardized with the heat soluble B. canis antigen indicates that this assay can be used as a reliable and practical method to confirm infection by this microorganism, as well as a tool for seroepidemiological studies.  相似文献   

不同南药多糖对鸡传染性法氏囊病毒VP2蛋白免疫原性的影响     

朱永健  那威  杨雨辉  胡日查  杨鑫  李承姮  周瑞刚  韩蒙蒙  陈云 《黑龙江畜牧兽医》2020,(9):119-123

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Sodium hydroxide or urea pretreatment of acerola (Malpighia emarginata) fruit residue increases dry matter degradability and reduces methane production in in vitro rumen fermentation     

Moura  Lais Micaelle Lopes  da Silva Pereira  Flávia Denise  de Lima  Patrícia Rodrigues  Nascimento  Júlio Cesar Silva  dos Santos de Oliveira  Andresca  de Souza Rodrigues  Rafael Torres  Ferreira  Clébio Pereira  Moraes  Salete Alves  Queiroz  Mário Adriano Ávila  Menezes  Daniel Ribeiro 《Tropical animal health and production》2020,52(5):2433-2441

Tropical Animal Health and Production - The aim of this study was to evaluate the effect of adding different concentrations of either urea or NaOH in dehydrated acerola (Malpighia emarginata) fruit...  相似文献   

Brain anatomy of two-toed sloth (Choloepus didactylus,Linnaeus, 1758): A comparative gross anatomical study of extant xenarthrans     

Letícia Menezes Freitas  Odeony Paulo dos Santos  André Luiz Quagliatto Santos  Fabiano Rodrigues de Melo  Leandro Silveira  Anah Tereza de Almeida Jácomo  Kleber Fernando Pereira  Fabiano Campos Lima 《Anatomia, histologia, embryologia》2020,49(1):130-143

The neural system plays an important role in understanding some features of animals. Anatomical complexity correlates with the increase of functional capacity. Xenarthrans include anteaters (Vermilingua), armadillos (Cingulata) and sloths (Folivora). This group is the base of eutherian mammals, and understanding the anatomy of its neural system could provide data for functional and evolutionary interpretations. The gross anatomy of the xenarthran brain is recorded. Four extant families of Pilosa and two families of Cingulata were sampled. Usual dissection procedures were used, and the brains were analysed macroscopically. The brain of two-toed sloth, three-toed sloth, six-banded armadillo, giant anteater and collared anteater are gyrencephalic. Pygmy anteater, nine-banded armadillo, great long-nosed armadillo, southern naked-tailed armadillo and giant armadillo are lissencephalic. In most species, the rhinal fissure presents two segments, rostral and caudal (except in Vermilingua and three-toed sloth). The diencephalon and brainstem present similar anatomy. The cerebellum is wide and presents four lobes (rostral, central, caudal and floccular). Its average volume is 12.16% (Folivora), 14.26% (Vermilingua) and 18.61% (Cingulata). Among these groups, there is a statistical difference between Folivora/Cingulata concerning the cerebellum average. The general pattern of the brain of the xenarthrans is similar to that of other mammals.  相似文献   

水稻种子播前处理质量变化研究   总被引：1，自引：0，他引：1  

那永光  陈淑洁  陈少龙 《现代化农业》2005,(3):19-20

目前,寒地水稻种子在播前按照选种、浸种、催芽、晾芽等步骤进行处理,其中在浸种步骤里,种子要经过急剧吸水的物理学吸胀和缓慢吸水的生物化学及水分散失的过程,在这些过程中,由于水分的变化,种子的质量(重量)性状将发生改变。系统地了解这些过程中水分的变化规律,特别是准确掌握播前种子水分变化与播量的关系,对提高技术的到位率是十分必要的。1　试验材料与方法按照千粒重、粒型的不同,参试品种选择有代表性的垦稻10号(千粒重23.78g,长粒)、空育131(千粒重24.64g,圆粒)、垦鉴稻2号(千粒重31.23g,圆粒)。经过脱芒、盐水选种(比重 1.13)…  相似文献   

晋西黄土区坡面糙率的研究   总被引：3，自引：0，他引：3  

张建军  纳磊  方家强 《北京林业大学学报》2007,29(1):108-113

坡面糙率是研究地表径流最为重要的参数之一,对坡面水文过程和土壤侵蚀过程模拟具有重要意义.该文采用野外实地冲刷水槽法,在测定不同地类地表糙率的基础上,研究了地表鲜草量、枯枝落叶量和地表硬度对地表糙率的影响,并探讨了地表糙率与地表径流泥沙含量的关系. 研究结果表明,不同地类地表糙率差异显著;地表糙率与鲜草量成线性关系;枯枝落叶对地表糙率的影响存在极值;地表糙率随土壤硬度的增加而减小,且成幂函数关系;地表径流的泥沙含量随地表糙率的增加而减小,二者成对数关系.   相似文献   

基于GWR模型降尺度模拟蒙古高原地区TRMM降水数据     

赵泽宇  秦福莹  那音太  那仁满都拉  郭恩亮  包玉海 《中国农业气象》2023,44(3):182-192

以多年月平均NDVI值高于0.1为阈值,对蒙古高原进行“植被区”与“非植被区”的子区域划分。在分析“植被区”植被对降水响应时滞性和“非植被区”陆地表面温度不同数据值与降水量相关性的基础上,子区域分别构建了TRMM3B43降水数据与海拔、坡度、坡向数据、归一化植被指数（NDVI）/陆地表面温度（LST）数据的地理加权回归（GWR）模型,得到区内2006-2015年每年5-10月1km空间分辨率的月降水量降尺度模拟数据,并利用区内141个气象站点数据对降尺度模拟数据进行精度验证。结果表明：（1）蒙古高原“植被区”植被对降水响应存在时滞性,约为一个月;“非植被区”多数月份白天与夜晚陆地表面温度差（LST＿D＿N）与降水量的相关性最显著。（2）降尺度模拟数据与气象站点数据具有较好的一致性,月尺度相关系数为0.83,各站点相关系数介于0.42～0.98。（3）在生长季、月平均尺度上,降尺度模拟数据具有较高精度,其中9月和10月数据精度优于TRMM 3B43数据。降尺度模拟数据整体精度较高,加之对原始数据在50°N以上未覆盖地区的填补以及空间分辨率的提高,可为区内水循环变化、农牧业生产、干旱监测等...  相似文献   

低氮高效红甜菜形态特征分析     

韩卓君  崔晶晶  潘恒艳  李有为  那明辉  宋柏权  周建朝  王秋红 《中国农学通报》2022,38(13):20-29

筛选低氮高效基因型红甜菜是提高氮素利用效率,减少氮污染的有效途径。采取液体培养方法,设置低氮(1.5 mmol/mL)、正常氮(5 mmol/mL)、高氮(10 mmol/mL)3种氮素水平处理,选用中国农业科学院甜菜研究所育种品系,通过生长指标统计、因子分析、聚类分析来确定评价指标,并筛选出耐低氮基因型。在低氮和高氮条件下,茎叶氮含量、茎叶氮素干物质生产效率、整株氮累积量、氮生理利用效率、整株氮素干物质生产效率差异性显著,变异系数分别为0.169~0.217和0.110~0.271。通过因子分析发现,在低氮和高氮条件下主成分培养情况基本相同,第一主成分是由根系氮含量、根系氮素干物质生产效率、整株氮素干物质生产效率、整株氮含量、氮生理利用效率决定,第一主成分所占的方差贡献率较大。综合红甜菜氮素吸收累积变异特征及因子分析,将茎叶氮含量、茎叶氮素干物质生产效率、整株氮累积量、氮生理利用效率、整株氮素干物质生产效率作为初步筛选红甜菜氮高效综合评价指标。再根据隶属函数法计算出的氮效率综合值和采用欧氏距离平方拟合的分层聚类分析,将整株氮素干物质生产效率作为筛选氮高效综合评价指标。最终筛选出对氮素高效吸收利用的材料（低氮高效型‘YeginaS2’、低氮低效高氮高效型‘CYCLHDAR’）,结合其株高、叶面积、叶柄长、根长等形态特征、干物质量及根冠比等实用特征,分析不同基因型红甜菜对土壤氮素吸收利用特点,进一步验证对增施氮肥反应敏感的低氮高效型、低氮低效高氮高效型品种。  相似文献   

食源性金黄色葡萄糖球菌耐氨基糖苷类药物基因的多重PCR快速检测技术     

战晓微  郑秋月  傅俊范  徐扬  那晗 《沈阳农业大学学报》2015,(1):31-36

为研究食源性金黄色葡萄球菌中MRSA及对氨基糖苷类庆大霉素、卡那霉素抗生素的耐药性情况,并建立快速检测鉴定金黄色葡萄球菌耐药性多重PCR方法。对金黄色葡萄球菌进行增菌培养,并提取DNA作为模板,以nuc基因作为菌属鉴定基因,mecA基因作为MRSA检测基因,aac(6')-aph(2')及aph(3')-Ⅲa基因作为耐氨基糖苷类庆大霉素、卡那霉素和妥布霉素的检测目的基因,建立四重PCR检测方法并进行特异性及灵敏度评价;利用所建立的方法检测19株金黄色葡萄球菌的分离株。结果表明:对19株金黄色葡萄球菌分离株的PCR检测结果与纸片扩散法的检测结果一致。所建立的多重PCR方法灵敏度高、特异性好,可以快速检测出MRSA,并对氨基糖苷类庆大霉素、卡那霉素、妥布霉素耐药菌株检测具有较高的准确率。  相似文献