Collecting plant genetic resources in Cuba     

Miguel Esquivel  Leonor Castiñeiras  Bartolomé Rodríguez  Karl Hammer 《Genetic Resources and Crop Evolution》1987,35(2):367-378

Summary After a short introduction into recent collecting activities in Cuba information is provided concerning the joint expedition of the Instituto de Investigaciones Fundamentales en Agricultura Tropical and the Zentralinstitut für Genetik und Kulturpflanzenforschung in the province of Holguín, Eastern Cuba, in February 1986 within the agreement between the Academy of Sciences of Cuba and the Academy of Sciences of the German Democratic Republic. Altogether 249 accessions of vegetables, pulses, cereals and other crops were collected. A remarkable diversity was found inCapsicum andPhaseolus. The material collected is important for breeding purposes.

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Sammlung pflanzlicher genetischer Ressourcen in Kuba

Zusammenfassung Nach einer kurzen Einführung in die Sammelarbeit der letzten Jahre in Kuba folgen Informationen über die gemeinsame Expedition des Instituto de Investigaciones Fundamentales en Agricultura Tropical und des Zentralinstituts für Genetik und Kulturpflanzenforschung, die im Rahmen des Abkommens zwischen der Akademie der Wissenschaften Kubas und der Akademie der Wissenschaften der Deutschen Demokratischen Republik im Februar 1986 durchgeführt wurde und in die Provinz Holguín, Ost-Kuba, führte. Insegsamt wurden 249 Proben von Gemüsen, Hülsenfrüchten, Getreiden und anderen Fruchtarten gesammelt. Eine bemerkenswerte Variation wurde beiCapsicum andPhaseolus gefunden. Das Sammelmaterial ist für Züchtungszwecke von Bedeutung.

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Improvement of table grapes quality and safety by the combination of modified atmosphere packaging (MAP) and eugenol, menthol, or thymol   总被引：1，自引：0，他引：1  

Valverde JM  Guillén F  Martínez-Romero D  Castillo S  Serrano M  Valero D 《Journal of agricultural and food chemistry》2005,53(19):7458-7464

Table grape is a nonclimacteric fruit that shows a rapid loss of quality during storage and is very susceptible to colonization by fungi, especially Botrytis cinerea, which is considered the most important disease of this commodity. To solve this problem, synthetic fungicides have been used, although legal restrictions and consumer's concern demand the search of other safe means. In the present paper, and as an alternative of synthetic fungicides, an active packaging to improve MAP effectiveness on preserving table grape (cv. Crimson Seedless) quality and safety was developed by the addition of 0.5 mL of eugenol, thymol, or menthol inside the packages. Packages were stored at 1 degree C for 35 days. The final gas composition inside the packages was 1.4-2.0 and 10.0-14.5 kPa of CO(2) and O(2), respectively, with no significant slight differences among treatments. Results showed that the addition of eugenol, thymol, or menthol improved the beneficial effect of MAP in terms of delaying weight loss and color changes, retarding degrees Brix/acidity ratio evolution, and maintaining of firmness. Thereafter, these treatments showed additional benefit in terms of delayed rates of rachis deterioration and berry decay. Finally, the total viable counts for both mesophilic aerobics and especially yeast and molds were significantly reduced in the grapes packaged with the natural antimicrobial compounds. All of the above effects led to maintenance of table grape quality and safety for longer storage periods (3 additional weeks as compared to controls under MAP only).  相似文献   

Chemical composition and antifungal activity of essential oil of Chrysactinia mexicana gray   总被引：1，自引：0，他引：1  

Cárdenas-Ortega NC  Zavala-Sánchez MA  Aguirre-Rivera JR  Pérez-González C  Pérez-Gutiérrez S 《Journal of agricultural and food chemistry》2005,53(11):4347-4349

The chemical composition of the essential oil of Chysactinia mexicana was analyzed by gas chromatography-mass spectrometry. Seventeen compounds were characterized; eucalyptol (41.3%), piperitone (37.7%), and linalyl acetate (9.1%) were found as the major components. The essential oil of leaves and piperitone completely inhibited Aspergillus flavus growth at relatively low concentrations (1.25 and 0.6 mg/mL, respectively).  相似文献   

Avascular necrosis of the femoral heads in a red panda (Ailurus fulgens fulgens): possible Legg-Calve-Perthes disease.     

María Delclaux  Covadonga Talavera  Manuel López  Jose Miguel Sánchez  María Isabel García 《Journal of zoo and wildlife medicine》2002,33(3):283-285

A 17-mo-old captive-born female red panda (Ailurus fulgens fulgens) presented with a sudden onset of lameness in its left hind leg was diagnosed radiographically as having possible severe, bilateral Legg-Calve-Perthes disease with fracture of the great trochanter of the left femur. Surgical repair of the fracture was performed using pins and a tension band wire through a lateral approach to the hip. This is the first case reported at Madrid Zoo-Aquarium, where 63 individuals have been bred over 15 yr.  相似文献   

Isolation of Babesia spp. from asymptomatic human beings     

B.Miguel Osorno  Carlos Vega  Miodrag Ristic  Carlos Robles  Samuel Ibarra 《Veterinary parasitology》1976,2(1):111-120

Babesiosis is a worldwide tick-borne protozoan disease of a variety of wild and domestic animals. For decades, various babesia strains were considered host-specific. However, babesiosis has been diagnosed as the cause of acute illness in 13 human beings in recent years, three of whom died. Studies of babesiosis in domestic animals indicate that for every clinically demonstrable case, there are hundreds of cases of latent infection. In an effort to determine if human babesia infections might be present in Mexico, we selected as our testing ground an endemic rural area along the Gulf Coast where continuous epizootics of equine, bovine, ovine and canine babesiosis were known to occur. Of the 101 individuals examined serologically by the indirect hemagglutination (IHA) test using Babesia canis as antigen, 38 reacted at titers from 1 : 10 to 1 : 80. Blood from these reactors was injected into splenectomized hamsters. Hamsters inoculated with blood from three of the individuals showed babesia in their peripheral blood. The growth of the organism was established by subpassages into additional hamsters. The persons from whom the organisms were isolated were asymptomatic for babesiosis. None of the 29 individuals residing in Mexico City, which represented the urban population in the survey, reacted in the IHA test. This is the first documented finding of the latent form of human babesiosis.  相似文献   

Comparative study of cattle antirabies vaccines. II. Protection conferred by alurabiffa, ERA and formidogel vaccines     

A R Netto  M R Nilsson  J de A C?rtes  M Mizuno  O Miguel 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1972,20(5):398-404

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Performance of a Johne's disease enzyme-linked immunosorbent assay adapted for milk samples from goats.     

Miguel Salgado  Elizabeth J B Manning  Michael T Collins 《Journal of veterinary diagnostic investigation》2005,17(4):350-354

Antibody detection-based tests for paratuberculosis offer speed and economy, 2 diagnostic test attributes important to animal industries with narrow profit margins. Application of such tests to individual milk samples instead of serum samples can further improve testing efficiency and decrease testing cost. Accuracy of a commercial bovine paratuberculosis enzyme-linked immunosorbent assay (ELISA) adapted for use on goat serum and milk samples was determined. Fecal, blood, and milk samples were collected from 159 goats belonging to 2 Wisconsin goat herds with a prior history of paratuberculosis and 1 herd of 50 goats from a paratuberculosis-free Wisconsin herd. Fecal samples were cultured using the modified BACTEC 12B media. Sera were tested according to the manufacturer's instructions for bovine samples. Milk samples were centrifuged and mixed with the ELISA kit's Mycobacterium phlei-containing diluent at a ratio of 1:2. Using fecal culture as the "gold standard," the sensitivity of the ELISA on goat serum was 64% and the sensitivity of the ELISA on goat milk was 48%. The milk ELISA had higher agreement with fecal culture results (kappa = 0.525) than the serum ELISA (kappa = 0.425). ELISA specificity was 100% on both serum and milk. Regression analysis also showed good correlation between serum and milk S/P values (r2 = 0.67). Although less sensitive, the ELISA on goat milk samples appears to offer a useful, low-cost alternative for detection of goats with paratuberculosis that have progressed to the stage of shedding M. paratuberculosis in their feces.  相似文献   

A PCR technique for the detection of Jaagsiekte sheep retrovirus in the blood suitable for the screening of ovine pulmonary adenocarcinoma in field conditions     

De Las Heras M  Ortín A  Salvatori D  Pérez de Villareal M  Cousens C  Miguel Ferrer L  Miguel Cebrián L  García de Jalón JA  Gonzalez L  Michael Sharp J 《Research in veterinary science》2005,79(3):201-264

Ovine pulmonary adenocarcinoma (OPA) is a naturally occurring contagious lung neoplasia caused by jaagsiekte sheep retrovirus (JSRV). Although no specific circulating antibodies against the virus can be detected in infected sheep, JSRV proviral DNA sequences can be found in peripheral blood leukocytes (PBLs) in clinically affected and in a proportion of in contact animals. In this study, existing hemi-nested PCR procedure is compared with a new one-step PCR technique that was developed to minimise potential DNA contamination and reduce sample and reagent handling. Different blood preparations were assessed and the best results were achieved on DNA prepared from buffy coat. The sensitivity of this PCR was lower in JSRV infected sheep without lesions of OPA than in clinically affected sheep, which indicate that this PCR may not be not fully appropriate for screening of individual sheep, but rather to provide results at flock level. This PCR is the only currently available blood test for detection of JSRV infected sheep and may be useful in epidemiological studies and in control programmes of OPA.  相似文献   

L-Bupivacaine 0.5% vs. racemic 0.5% bupivacaine for caudal epidural analgesia in horses     

Derossi R  Miguel GL  Frazílio FO  Nunes DB  Kassab TA 《Journal of veterinary pharmacology and therapeutics》2005,28(3):293-297

Bupivacaine is available as a racemic mixture of its enantiomers, d -bupivacaine and l -bupivacaine (LB). The aim of this randomized, double-blind study was to investigate the clinical efficacy and safety of S(−)-bupivacaine compared with standard racemic bupivacaine (RB) in horses under caudal epidural analgesia. Two treatments were administered to each horse, with a 2-week interval between subsequent treatments. Treatment 1 consisted of 0.5% LB at a dose of 0.06 mg/kg of body weight, and treatment 2 consisted of 0.5% RB at a dose of 0.06 mg/kg of body weight. Epidural injections were given in all animals between the first and second coccygeal vertebra. Heart rate (HR), arterial pressures, respiratory rate (RR), rectal temperature (RT), analgesia, and motor blocking were determined before drug administration (basal) and 5, 10, 15 and 30 min after drug administration, and at 30 min intervals thereafter. There were no significant differences between the two treatments in the quality of sensory and motor block. The duration of analgesia was 320 ± 30 min (mean ± SD) for RB and 360 ± 42 min for LB. HRs and RRs, arterial pressures and RT did not change ( P  < 0.05) significantly from basal values after epidural administration of LB or RB. This study supports that 0.5% LB is an effective alternative to RB in caudal epidural analgesia in conscious, standing horses. The use of LB vs. RB warrants further investigation, particularly for long-lasting surgery in the perineal region.  相似文献   

Computed tomographic anatomy of the head of the loggerhead sea turtle (Caretta caretta)     

Arencibia A  Rivero MA  De Miguel I  Contreras S  Cabrero A  Orós J 《Research in veterinary science》2006,81(2):165-169

The heads of three loggerhead sea turtles were disarticulated and imaged immediately to minimize postmortem changes and then frozen and sectioned. For computed tomography (CT) imaging, the heads were positioned in ventral recumbency. Transverse CT images with soft-tissue window were obtained from the olfactory sac region to the temporomandibular joint region. After CT imaging, the heads were sectioned and the gross sections were compared to CT images, to assist in the accurate identification of the anatomic structures. Different clinically relevant anatomic structures were identified and labelled in two series of photographs (CT images and anatomic cross-sections). CT images provided good differentiation between the bones and the soft tissues of the head. The information presented in this paper should serve as an initial reference to evaluate CT images of the head of the loggerhead sea turtle and to assist in the interpretation of lesions of this region.  相似文献