Effect of dietary zinc source and method of oral administration on performance and tissue trace mineral concentration of broiler chicks.   总被引：3，自引：0，他引：3  

M Sadoval  P R Henry  R C Littell  R D Miles  G D Butcher  C B Ammerman 《Journal of animal science》1999,77(7):1788-1799

Two experiments were conducted with chicks to examine the effect of high dietary levels of soluble sources of Zn on tissue Zn, Cu, and Fe concentrations as influenced by two methods of oral Zn administration from 14 to 21 d of age. Treatments included the basal diet (62 ppm Zn), basal diet supplemented with 1,000 ppm Zn from Zn sulfate, acetate, or chloride fed continuously, or basal diet plus crop intubation with a single oral dose of water (control) or 1,000 ppm Zn dietary equivalent from the sources based on feed intake from the previous day. In Exp. 2, crop-intubated doses were administered daily from 14 to 21 d of age. In Exp. 1, chicks given Zn by gavage decreased (P < .0001) feed intake at 24 h after oral dose compared with chicks fed either the control or high-Zn diets. After the gavage dosing stopped, feed intake was similar among treatments. Bone Zn was increased (P < .0001) by Zn source and was greater at 24 than at 168 h after dosing by gavage. In chicks given a single gavage dose of Zn, liver and kidney Zn measured at 24 h after oral dosing was greater (P < .0001) than at 168 h. In birds given a single oral dose of Zn, time x Zn source interactions were observed in pancreas (P < .0001), mucosal cells (P < .01), and remaining intestinal tract segments (P < .001). In Exp. 2, greater bone, pancreas, kidney (P < .0001), and liver (P < .001) Zn accumulations were observed in chicks given daily gavage doses of Zn compared with those fed Zn in diets. Zinc from the four sources was absorbed and stored in tissues to a similar extent.  相似文献   

Aerial applications of pesticides in the United Kingdom: 1978 to 1998     

Oliver L Wardman  Miles R Thomas 《Pest management science》2000,56(3):237-244

The aerial application of pesticides increased rapidly following the end of the Second World War, as, along with the development of a wider range of synthetic pesticides, more planes and pilots became available. It also became clear that new and previously inaccessible areas could be sprayed from the air. By the 1980s there were around 100 planes in the United Kingdom (UK) capable of spraying pesticides. However, increasing environmental pressure brought about the introduction of tougher legislation to control pesticide usage. The Food and Environment Protection Act 1985 and the Control of Pesticides Regulations 1986 (as amended) introduced tight controls over the application of pesticides from the air. Aerial application of pesticides has been monitored each year by the UK Ministry of Agriculture, Fisheries and Food (MAFF) since 1978, during which time the area treated has decreased significantly. This has been partly as a result of changes in legislation but also due to technological improvements in conventional crop‐protection methods. The area treated from the air in the UK has never risen above 2.5% of the area treated from the ground and in 1998 had fallen to less than 0.04% of the total area treated with pesticides. It is likely that this figure will continue to fall in the future. By 1998, the only pesticides to be sprayed from the air comprised fungicides, herbicides and insecticides. As a group, herbicides comprised the greatest area of treatment and the only target to show an increase in area treated in recent years has been bracken (Pteridium aquilinum (L) Kuhn). © Crown copyright 2000. Reproduced with the permission of Her Majesty's Stationery Office.  相似文献   

Carotenoid composition of a New Zealand (Evechinus chloroticus) and an Australian (Heliocidaris erythrogramma) sea urchin in relation to gonad colour          下载免费PDF全文

Jodi Brewster  Daniel Garama  Miles Lamare  Alan Carne 《Aquaculture Research》2018,49(8):2751-2762

Sea urchins are commercially harvested for their edible gonads, also known as roe or uni. Most species, particularly those from the Northern hemisphere are reported to exhibit limited variation in gonad colour, which is proposed to be due to carotenoid pigments. The commercially harvested species from New Zealand, Evechinus chloroticus, and from Australia, Heliocidaris erythrogramma, however, exhibit considerable gonad colour variation, ranging from a culinary desirable light yellow/orange to an undesirable brown. Gonads from E. chloroticus and H. erythrogramma were characterized spectrophotometrically and the carotenoid content extracted and analysed by reversed‐phase high‐performance liquid chromatography. The main carotenoid found in the gonads of both species was 9′‐cis‐echinenone, with lower levels of all‐trans‐echinenone, lutein and isozeaxanthin. Carotenoid composition contributed to the yellow/orange colour components of the gonads, but was not correlated with the darker roe coloration observed in some E. chloroticus individuals. Our results highlight important considerations relating sea urchin gonad coloration to carotenoid content and as such will provide useful information for the future aquaculture and processing of these two species.  相似文献   

Kinetics of substrate oxidation and hydrogen peroxide production by Mycoplasma mycoides subsp. mycoides Large Colony (LC) type and Mycoplasma mycoides subsp. capri     

Masoud Shahram  Robin A.J. Nicholas  Roger J. Miles  Ann P. Wood  Donovan P. Kelly   《Research in veterinary science》2009,87(3):364-366

Mycoplasma mycoides subsp. mycoides Large Colony (LC) type is a pathogen of goats causing contagious agalactia and respiratory disease, found on all continents where small ruminants are kept. It shares close genetic characteristics with M. mycoides subsp. capri. Substrate oxidation by 22 strains of M. mycoides subsp. mycoides LC from nine countries was compared with that of eight strains of M. mycoides subsp. capri from five countries. There was considerable similarity in the substrates used, but substrate saturation coefficients (K_s) varied for different substrates. Substrate utilization patterns and K_s values did not (1) significantly differentiate the LC strains from each other, (2) show any correlation with geographical origin, or (3) distinguish the LC strains from the capri strains. These results support previous studies justifying the reclassification of these subspecies as a single species.  相似文献   

Identification of fatty acid esters of pectenotoxin-2 seco acid in blue mussels (Mytilus edulis) from Ireland     

Wilkins AL  Rehmann N  Torgersen T  Rundberget T  Keogh M  Petersen D  Hess P  Rise F  Miles CO 《Journal of agricultural and food chemistry》2006,54(15):5672-5678

Pectenotoxins from marine dinoflagellates of the genus Dinophysis are rapidly hydrolyzed by many shellfish to give pectenotoxin-2 seco acid, which isomerizes to 7-epi-pectenotoxin-2 seco acid. Three series of fatty acid esters of pectenotoxin-2 seco acid (PTX-2 seco acid) and 7-epi-PTX-2 seco acid were detected by LC-MS analysis of extracts from blue mussels (Mytilus edulis) from Ireland. The locations of the fatty acid ester linkages were identified by a combination of LC-MSn in positive- and negative-ion modes, LC-MS analysis of the products from reaction of the esters with sodium periodate, and NMR analysis of purified samples of the two most abundant ester derivatives. The 37-O-acyl esters of PTX-2 seco acid were the most abundant, followed by the corresponding 11-O-acyl esters, accompanied by low levels of the 33-O-acyl esters. The most abundant fatty acid esters in the fractionated sample were, in order, the 16:0, 22:6, 14:0, 16:1, 18:4, and 20:5 fatty acids, although a wide array of other PTX-2 seco acid fatty acid esters were also present at low levels.  相似文献   

Production and isolation of azaspiracid-1 and -2 from Azadinium spinosum culture in pilot scale photobioreactors     

T Jauffrais  J Kilcoyne  V Séchet  C Herrenknecht  P Truquet  F Hervé  JB Bérard  C Nulty  S Taylor  U Tillmann  CO Miles  P Hess 《Marine drugs》2012,10(6):1360-1382

Azaspiracid (AZA) poisoning has been reported following consumption of contaminated shellfish, and is of human health concern. Hence, it is important to have sustainable amounts of the causative toxins available for toxicological studies and for instrument calibration in monitoring programs, without having to rely on natural toxin events. Continuous pilot scale culturing was carried out to evaluate the feasibility of AZA production using Azadinium spinosum cultures. Algae were harvested using tangential flow filtration or continuous centrifugation. AZAs were extracted using solid phase extraction (SPE) procedures, and subsequently purified. When coupling two stirred photobioreactors in series, cell concentrations reached 190,000 and 210,000 cell · mL(-1) at steady state in bioreactors 1 and 2, respectively. The AZA cell quota decreased as the dilution rate increased from 0.15 to 0.3 day(-1), with optimum toxin production at 0.25 day(-1). After optimization, SPE procedures allowed for the recovery of 79 ± 9% of AZAs. The preparative isolation procedure previously developed for shellfish was optimized for algal extracts, such that only four steps were necessary to obtain purified AZA1 and -2. A purification efficiency of more than 70% was achieved, and isolation from 1200 L of culture yielded 9.3 mg of AZA1 and 2.2 mg of AZA2 of >95% purity. This work demonstrated the feasibility of sustainably producing AZA1 and -2 from A. spinosum cultures.  相似文献   

Evaluation of soybean for resistance to soybean rust in Vietnam     

T.A. Pham  C.B. Hill  M.R. Miles  B.T. Nguyen  T.T. Vu  T.D. Vuong  T.T. VanToai  H.T. Nguyen  G.L. Hartman 《Field Crops Research》2010

Soybean rust, caused by Phakopsora pachyrhizi Sydow, is a severe foliar disease of soybean [Glycine max (L.) Merr.] that occurs throughout most soybean producing regions of the world. The objective of this research was to evaluate selected soybean genotypes for resistance to soybean rust in Vietnam. Five field experiments in Vietnam were completed from 2006 to 2009. The area-under-the-disease-progress-curve (AUDPC) was calculated for each soybean genotype based on four disease assessments taken during the reproductive growth stages. AUDPC units among soybean genotypes in each experiment differed (P < 0.05). Over the five experiments, the resistant check DT 2000 was most often the genotype with the lowest AUDPC units while the sources of rust resistance (Rpp1-5) did not always have low AUDPC units in each experiment, although PI 230970 (Rpp2) appeared to be more stable. A few genotypes with non-characterized genes for resistance, such as PI 398998, PI 437323, and PI 549017, had the lowest AUDPC units in at least one of the experiments. These genetic resources may be useful for host plant resistance studies and breeding soybeans for rust resistance in Vietnam and other locations like Brazil and the United States that have more recently been inundated with soybean rust. A significant (P < 0.001) experiment × genotype interaction was found when the AUDPC data of 14 soybean genotypes tested in Experiments 1, 2, and 3 were combined and analyzed. This result indicates the potential importance of changing fungal races and/or biotypes that occur in the rust population.  相似文献   

Centuries-old logging legacy on spatial and temporal patterns in understory herb communities     

Julie L. Wyatt  Miles R. Silman 《Forest Ecology and Management》2010

Understory herb communities in the Southern Appalachians are among the highest biodiversity plant communities in North America. In the mid-1990s, a debate began over whether understory herb communities recover to their pre-disturbance states following logging. Studies showing reduced herb-layer diversity in previously logged forests were criticized for not accounting for intersite environmental heterogeneity. More recent studies have addressed environmental heterogeneity, but have neglected long-term recovery by using “mature forests” as young as 80 years old as the benchmark for diversity comparison, even though old growth stands have disturbance return intervals exceeding 500 years. Here we address concerns clouding previous studies of high-diversity Appalachian herb communities and investigate their long-term recovery by comparing paired sites of old growth forest and forest logged 100–150 years ago. We found that species richness and individual abundance is greater in old growth forests than mature forests and that species composition differed significantly between the two. Turnover in species among old growth and mature forests accounted for 11% of the total species richness and was significantly greater than expected. Species turnover at intermediate (5–50 m) and landscape-scales (>10 km) contributed the most towards total species richness. Herb communities in rich cove forests have successional trajectories that exceed 150 years, with important community changes still occurring long after the forest returns to what has been previously termed a “mature” state. To conserve the diverse herb layer, we conclude that mature forest stands are too young to serve as baselines for recovery, landscape-scale preservation of multiple forest stands is needed to maximize species richness, and maintaining 100–150-year logging rotations will likely lead to loss of biodiversity.  相似文献   

Developing an Integrated Method of Rice Insect Pest Control     

M. D. Pathak  V. A. Dyck 《国际虫害防治杂志》2013,59(4):534-544

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Improved isolation procedure for azaspiracids from shellfish, structural elucidation of azaspiracid-6, and stability studies     

Kilcoyne J  Keogh A  Clancy G  LeBlanc P  Burton I  Quilliam MA  Hess P  Miles CO 《Journal of agricultural and food chemistry》2012,60(10):2447-2455

Azaspiracids are a group of lipophilic polyether toxins produced by the small dinoflagellate Azadinium spinosum. They may accumulate in shellfish and can result in illnesses when consumed by humans. Research into analytical methods, chemistry, metabolism, and toxicology of azaspiracids has been severely constrained by the scarcity of high-purity azaspiracids. Consequently, since their discovery in 1995, considerable efforts have been made to develop methods for the isolation of azaspiracids in sufficient amounts and purities for toxicological studies, in addition to the preparation of standard reference materials. A seven-step procedure was improved for the isolation of azaspiracids-1-3 (1, 2, and 3) increasing recoveries 2-fold as compared to previous methods and leading to isolation of sufficiently purified azaspiracid-6 (6) for structural determination by NMR spectroscopy. The procedure, which involved a series of partitioning and column chromatography steps, was performed on 500 g of Mytilus edulis hepatopancreas tissue containing ~14 mg of 1. Overall yields of 1 (52%), 2 (43%), 3 (43%), and 6 (38%) were good, and purities were confirmed by NMR spectroscopy. The structure of 6 was determined by one- and two-dimensional NMR spectroscopy and mass spectrometry. The stability of 6 relative to 1 was also assessed in three solvents in a short-term study that demonstrated the greatest stability in aqueous acetonitrile.  相似文献