Demonstration of Sugar Residues in the Ultimobranchial Tubule and Thyroid C-Cells of the Rat using Peroxidase Labelled Lectins     

A. M. Moreno    I. Martin-Lacave  C. Montero    A. Gomez-Pascual  A. Fernandez  H. Galera 《Anatomia, histologia, embryologia》1989,18(2):114-121

The distribution of various sugars, that contribute to the composition of the carbohydrate components of the glycoprotein in the ultimobranchial tubule and the thyroid C-cells has been studied, using eight peroxidase-labelled lectins (Dolichos biblorus, Glycine max, Bauhinia purpurea, Helix pomatia, Arachis hipogaea, Ulex europaeus I, Limax flavus and Sophora japonica). The results obtained seem to show that there are receptors for Dolichos biflorus, Glycine max, Bauhinia purpurea, Helix pomatia, Arachis hipogaea, Ulex europaeus I and Limax flavus in the ultimobranchial tubule, suggesting the existence of N-acetylgalactosamine, galactose, L-fucose and sialic acid. However, the C-cells show only a positive reaction with Ulex europaeus I indicating the existence of fucosyl residues in those cells. All these findings support the contention that the ultimobranchial body might contribute decisively not only to the formation of C-cells but also, partially, to the constitution of certain follicular cells.  相似文献   

Histochemical and morphometric studies of the cranial tibial muscle in dogs of different dynamic aptitudes (greyhound, German shepherd and fox terrier)     

P Rodríguez-Barbudo  R Vaamonde  E Agüera  M Carpio  F Moreno  S Fuentes  A Robina 《Anatomia, histologia, embryologia》1984,13(4):300-312

A histochemical and morphometric study of the cranial tibial muscle of the greyhound, the German shephard and the fox terrier was carried out using hematoxyline-eosin, PAS, Gomori trichrome, SDH, LDH, NADH-tr and ATPase staining (with pH adjustments to both acidic and basic levels), with the goal of comparing the proportion and distribution of type I and type II fibers within the muscle. Significant differences were found between the three breeds of dogs. It is suggested that these differences are related to the locomotor activities of the three breeds.  相似文献   

Comparing alternative definitions of the contemporary group effect in Avilena Negra Iberica beef cattle using classical and Bayesian criteria     

Carabaño MJ  Moreno A  López-Romero P  Díaz C 《Journal of animal science》2004,82(12):3447-3457

Data on weaning weight from 12,740 animals were used to compare different definitions of contemporary groups (CG) for the genetic evaluation of the Avilena Negra Iberica beef cattle breed. Six alternative definitions for the CG effect were considered: herd-year-season of calving (HYS), with seasons defined according to the four natural seasons; herd-year-month of calving (HYM); herd clusters of 30 d (HC30-30) or 90 d (HC90-90); and adaptive herd clusters with two time limits, 30 and 90 d (HC30-90), and 30 and 180 d (HC30-180). A minimum of five observations in each CG class was required. This rendered substantial differences in loss of information, ranging from 0.7% of the total number of records for HC30-180 to 14% for HYM. Several classical statistics and Bayesian criteria for statistical model comparison were used. The use of classical criteria, such as the between- and within-CG variation and the accuracy of prediction, can be controversial because of their dependency on the unknown variance components. Residual variance decreased with the decrease in time span associated with the definition of CG. This was expected in this population because environmental conditions are highly variable throughout the year. However, estimates of the additive genetic variance for direct effects, which should not be affected by the definition of CG, were substantially larger for definitions involving larger time periods (HYS, HC90-90). When parameters used in the current evaluation procedure were used with all data sets, CG involving 30 d (HYM and HC30-30) were optimal in terms of providing the lowest/largest within-/between-CG variation. On the other hand, CG involving 90 d (HYS and HC90-90) yielded the poorest within-/between CG variation, with only a slight improvement of accuracy of prediction of direct genetic values over the other definitions. Bayes factors and cross-validation predictive densities allowed for improved discrimination among models. Models including CG spanning 30 d were more plausible and showed better predicting ability than models spanning 90 d. Adaptive CG showed intermediate results. Overall, it seems that average time span rendered by the different definitions had a major effect on the ranking of models. However, from the breeder's point of view, the loss of information associated with definitions involving shorter periods of time, such as HYM or HC30-30, might be unacceptable.  相似文献   

Detection and differentiation of porcine circoviruses in Brazilian pigs     

Castro AM  Cortez A  Ruiz VL  Leomil H  Moreno AM  Doto DS  Richtzenhain LJ 《The Veterinary record》2004,154(23):728-729

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Rough vaccines in animal brucellosis: structural and genetic basis and present status   总被引：14，自引：0，他引：14  

Moriyón I  Grilló MJ  Monreal D  González D  Marín C  López-Goñi I  Mainar-Jaime RC  Moreno E  Blasco JM 《Veterinary research》2004,35(1):1-38

Brucellosis control and eradication requires serological tests and vaccines. Effective classical vaccines (S19 in cattle and Rev 1 in small ruminants), however, induce antibodies to the O-polysaccharide of the lipopolysaccharide which may be difficult to distinguish from those resulting from infection and may thus complicate diagnosis. Rough attenuated mutants lack the O-polysaccharide and would solve this problem if eliciting protective immunity; the empirically obtained rough mutants 45/20 and RB51 have been used as vaccines. Strain 45/20 is reportedly unstable and it is not presently used. RB51 is increasingly used instead of S19 in some countries but it is rifampicin resistant and its effectiveness is controversial. Some controlled experiments have found good or absolute protection in adult cattle vaccinated orally (full dose) or subcutaneously (reduced dose) and in one field experiment, RB51 was reported to afford absolute protection to calves and to perform better than S19. Controlled experiments in calves, however, have shown reduced doses of RB51 to be ineffective, full doses only partially effective, and RB51 less effective than S19 against severe challenges. Moreover, other observations suggest that RB51 is ineffective when prevalence is high. RB51 is not useful in sheep and evidence in goats is preliminary and contradictory. Rough mutants obtained by molecular biology methods on the knowledge of the genetics and structure of Brucella lipopolysaccharide may offer alternatives. The B. abortus manBcore (rfbK) mutant seems promising in cattle, and analyses in mice suggest that mutations affecting only the O-polysaccharide result in better vaccines than those affecting both core and O-polysaccharide. Possible uses of rough vaccines also include boosting immunity by revaccination but solid evidence on its effectiveness, safety and practicality is not available.  相似文献   

Analysis of the toxic potential of Palicourea corymbifera (Müll. Arg.) Standl. in laboratory animals     

Ribeiro de Assis JC  Suffredini IB  Moreno PR  Young MC  Varella AD  Younes RN  Bernardi MM 《Research in veterinary science》2006,80(2):209-217

Palicourea species may produce bovine toxicity. Palicourea corymbifera grows in terra firme forests within the Amazon rain forest and in Tropical America, particularly in spots that gave place to gazing areas. The lyophilized extract done with the aerial organs of P. corymbifera were analyzed in male and female mice. Results revealed a significant toxicity: LD50 was 1.10 (1.04-1.15)g/kg for male mice, and 1.05 (1.00-1.10)g/kg for female mice. Locomotion was affected as well as there were reflexes linked to environmental stimuli in addition to changes in posture. Progressive central nervous system stimulus signs such as trembling and convulsions were detected, the latter followed by the animal's death. Macroscopic histopathological exams performed on the liver, kidneys and lungs of mice submitted to necropsy did not indicate the existence of lesions. General activity of animals, measured in an open field, was reduced as a result of the administration of the extract. Duration of locomotion and rearing frequency were reduced, in opposition to an increase in the duration of immobility. Thin layer chromatography analysis showed that monofluoroacetic acid is present in the lyophilized extract, but other qualitative techniques as gas chromatography/mass spectrometry and 19F nuclear magnetic resonance showed that the MFAA was not present in the extract, and that the toxicity is related to other compound, although the toxic profile is very similar to that of MFAA. P. corymbifera was shown to be significantly toxic to laboratory animals and investigation of the possible toxic substance shall be done.  相似文献   

Development of an indirect enzyme-linked immunosorbent assay for detecting equine serum antibodies to the lipopolysaccharide of Salmonella abortusequi     

Gall D  Nielsen K  Bermudez RM  Muñoz del Real MC  Halbert G  Groulx R  Moreno F  Chow EY  Checkley SL 《Research in veterinary science》2006,81(2):215-217

An indirect enzyme-linked immunosorbent assay (IELISA) was developed for the detection of equine serum antibodies to lipopolysaccharide of Salmonella enterica subsp. enterica serovar Abortusequi (LPS), a causative organism of Equine Paratyphoid. The data presented demonstrates that horses immunized with S. abortusequi LPS developed antibodies detectable by the IELISA. By comparison, the tube agglutination test (TAT) did not detect antibody to S. abortusequi LPS as consistently as the IELISA. The data suggests that the IELISA may be a more suitable test for the detection of serum antibodies to S. abortusequi than the TAT.  相似文献   

Evaluation of the health of free-ranging greater rheas (Rhea americana) in Argentina     

Uhart M  Aprile G  Beldomenico P  Solís G  Marull C  Beade M  Carminati A  Moreno D 《The Veterinary record》2006,158(9):297-303

The health of 22 free-ranging adult rheas (Rhea americana) examined and sampled during a translocation/reintroduction project and six juvenile rheas kept in semicaptivity was investigated, and details of their haematology and plasma biochemistry are presented. Serological testing for antibodies to infectious agents was negative for infectious laryngotracheitis, avian adenovirus, avian influenza, avian reovirus, infectious bursal disease, infectious bronchitis virus, paramyxovirus types 1, 2, and 3, fowlpox and Salmonella Pullorum. Antibodies to Chlamydophila species were found in 25 of 27 of the birds, and 22 of 25 had antibodies to Aspergillus species. Ova of gastrointestinal nematodes of the genus Capillaria were identified, and the anoplocephalid cestode Monoecocestus cf rheiphilus was identified in R americana for the first time.  相似文献   

Comparative efficacy of two single-dose bacterins in the control of Mycoplasma hyopneumoniae in swine raised under commercial conditions in Brazil     

Baccaro MR  Hirose F  Umehara O  Gonçalves LC  Doto DS  Paixão R  Shinya LT  Moreno AM 《Veterinary journal (London, England : 1997)》2006,172(3):526-531

A field study was conducted in Brazil to evaluate the efficacy of single vaccination of pigs with two bacterins to prevent Mycoplasma hyopneumoniae lung lesions. The first (T1) treatment group (174 pigs) was injected with 2 mL of saline solution; group T2 (177 pigs) with 2 mL of bacterin A, and group T3 (174 pigs) with 2 mL of bacterin B. On days-on-test (DOT) 0, 35, 66, 97 and 125, blood samples and tonsil swabs were collected from selected pigs for antibody determination (indirect ELISA) and PCR assay for the presence of M. hyopneumoniae. Pigs were slaughtered on DOT 126-129 and lung lesions were scored blindly. Bacterin A vaccinated pigs had significantly (P < or = 0.05) lower lung lesion scores (0.2%) than bacterin B (0.4%) or saline-treated pigs (1.2%); there was also a significantly lower (P < or = 0.05) number of pigs with lung lesions (27.1%), than bacterin B (38.2%) or saline-treated (55.4%) pigs. The two vaccines had similar (P>0.05) results in terms of mean weight gain, average daily weight gain, feed efficiency, frequency of PCR positives, and there was similar antibody conversion (ELISA). It was concluded that although the productivity parameters and antibody conversions were similar, bacterin A was more effective in preventing and reducing the severity of lung lesions than bacterin B.  相似文献   

Calculation of diagnostic parameters of advanced serological and molecular tissue-print methods for detection of Citrus tristeza virus: a model for other plant pathogens   总被引：1，自引：0，他引：1  

Vidal E  Yokomi RK  Moreno A  Bertolini E  Cambra M 《Phytopathology》2012,102(1):114-121

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