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61.
This study was carried out to evaluate the effects of the sperm cell dose and semen backflow on the pregnancy rate and number of embryos of sows inseminated once at 0-24 h before ovulation, using an intrauterine technique. The results were analysed from a total of 211 sows assigned to three groups inseminated with doses of 0.25 x 10(9) (T1), 0.5 x 10(9) (T2) and 1.0 x 10(9) (T3) spermatozoa. Semen backflow was observed in 95% of the females (143/151) evaluated for this purpose. The percentage of semen backflow is close to two-third of the volume and the percentage of sperm is around 15% of the infused sperm dose. Intrauterine insemination can be successfully performed provided that at least 0.5 billion of sperm cell dose is infused at an interval of 0-24 h before ovulation.  相似文献   
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The presentation, treatment and outcomes of 33 ingluvial fibrous foreign bodies in cockatiels (Nymphicus hollandicus) are described. Vomiting, lethargy and weight loss were the most common presenting signs. Diagnosis was made on palpation of a mass in the crop (ingluvies). Both surgical and non‐surgical treatment regimens were evaluated. The overall mortality rate was 33%, suggesting fibrous materials are not safe for cockatiels to groom or ingest and should not be offered as cage accessories.  相似文献   
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We assessed shrew (soricids) response to coarse woody debris (CWD) manipulations in managed upland loblolly pine (Pinus taeda) stands in the upper Coastal Plain of South Carolina over multiple years and seasons. Using a completely randomized block design, we assigned one of the following treatments to 12, 9.3-ha plots: removal (n = 3; all CWD ≥ 10 cm in diameter and ≥60 cm long removed), downed (n = 3; 5-fold increase in volume of down CWD), snag (n = 3; 12-fold increase in standing dead CWD), and control (n = 3; unmanipulated). Therein, we sampled shrews during winter, spring, and summer seasons, 2003–2005, using drift-fence pitfall arrays. During 1680 drift-fence plot nights we captured 253 Blarina carolinensis, 154 Sorex longirostris, and 51 Cryptotis parva. Blarina carolinensis capture rate was greater in control than in snag treatments. Sorex longirostris capture rate was lower in removal than downed and control plots in 2005 whereas C. parva capture rate did not differ among treatments. Overall, the CWD input treatments failed to elicit the positive soricid response we had expected. Lack of a positive response by soricid populations to our downed treatments may be attributable to the early CWD decay stage within these plots or an indication that within fire-adapted pine-dominated systems of the Southeast, reliance on CWD is less than in other forest types.  相似文献   
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Central Greenland ice cores provide evidence of abrupt changes in climate over the past 100,000 years. Many of these changes have also been identified in sedimentary and geochemical signatures in deep-sea sediment cores from the North Atlantic, confirming the link between millennial-scale climate variability and ocean thermohaline circulation. It is shown here that two of the most prominent North Atlantic events-the rapid warming that marks the end of the last glacial period and the Bolling/Allerod-Younger Dryas oscillation-are also recorded in an ice core from Taylor Dome, in the western Ross Sea sector of Antarctica. This result contrasts with evidence from ice cores in other regions of Antarctica, which show an asynchronous response between the Northern and Southern Hemispheres.  相似文献   
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The aim of the present study was to examine the role of oxytocin (OT) in the progesterone (P4) and prostaglandins (PGs) pathway to induce oocyte meiotic resumption. Cumulus–oocyte complexes were co‐cultured with follicular hemisections for 15 h to determine the effects of different doses of OT or atosiban (ATO; oxytocin receptor antagonist) on oocyte meiotic resumption. In another experiment, we examined the effect of the interaction between P4, OT and PGs on the regulatory cascade of the oocyte meiotic resumption. Oxytocin at 1 μm was effective in inducing meiotic resumption in oocytes co‐cultured with follicular cells (84.0%), not differing from the positive control group (74.4%). Atosiban inhibited in a dose‐dependent manner the positive effect of OT on the meiotic resumption (27.6% metaphase I with 10 μm of ATO, which did not differ from the 25.5% of the negative control group). Furthermore, a third experiment showed that P4 was able to induce oocyte meiotic resumption, which was inhibited by ATO. However, the OT positive effect was not blocked by mifepristone (P4 antagonist), but was inhibited by indomethacin (a non‐selective PTGS2 inhibitor). Collectively, these data suggest a sequential role of P4, OT and PGs in the induction of oocyte meiotic resumption.  相似文献   
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动物疫苗及其合理使用(一)   总被引:2,自引:0,他引:2  
疫苗接种是预防动物传染病发生的重要举措。依据免疫学理论,疫苗作为抗原,通过激发动物机体免疫系统,产生特异的体液免疫和细胞免疫,从而获得针对某种疾病的特异性免疫。疫苗一般分为细菌疫苗、病毒疫苗和类毒素疫苗等。用于防病治病的各种疫苗、菌苗又是特殊的生物药品,与普通化学药品具有完全不同的成分、功能和特性,疫苗中含有多种蛋白质(除细菌或病毒外,有的还含有保护剂、鸡胚尿囊液或细胞培养液),它们均适合许多病原体的生长,有些制品还是活的微生物,一旦污染后果不堪设想。疫苗在保存、运输和使用时有其特别要求。如果方法不当,不但会使活性物质失活,起不到免疫防病作用;而且还可能适得其反,引发动物新的疾病,造成重大损失。  相似文献   
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