Evaporative cooling in late gestation heat‐stressed Murrah buffaloes increases efficiency of next reproductive cycle          下载免费PDF全文

O Aarif  A Aggarwal  AA Sheikh 《Reproduction in domestic animals》2018,53(1):249-255

Evaporative cooling during late gestation period improves post‐partum reproductive performance in Murrah buffaloes. To prove this hypothesis, sixteen pregnant dry Murrah buffaloes at sixty days pre‐partum were selected and divided into two groups of eight animals each. Group 1 of buffaloes (Cooled/CL) was managed under fan and mist cooling during dry period, whereas second group of buffaloes (non‐cooled/NCL) remained without the provision of cooling. After parturition, all the animals were managed under evaporative cooling till the end of experimental period. Reproductive performance in cooled (CL) and non‐cooled (NCL) groups, respectively, viz. 1st and 2nd ovulation from calving (48.63 ± 2.41, 69.25 ± 2.34 days and 57.75 ± 3.35, 93.63 ± 2.84 days); calving to conception interval (117.88 ± 4.21 days and 117.88± 4.21 days); conception rate (87.5% ± 2.16% and 57% ± 2.26%); and follicular diameter at the time of 1st and 2nd ovulation (14.84 ± 0.16, 15.75 ± 0.13 mm and 12.65 ± 0.13, 13.35 ± 0.11 mm) varied significantly (p < .05). Total peak oestrogen concentration was significantly (p < .05) higher in cooled (26.7 ± 1.32 pg/ml) relative to non‐cooled (20.7 ± 1.22 pg/ml) buffaloes. Time from onset of oestrus to ovulation varied significantly (p < .05) in cooled (32 ± 2.22 hr) and non‐cooled (40 ± 2.86 hr) buffaloes. The peak progesterone concentration reached to (4.25 ng/ml) in cooled group and (4.16 ng/ml) in non‐cooled group after first ovulation.  相似文献   

Susceptibility of Tuta absoluta (Lepidoptera: Gelechiidae) Brazilian populations to ryanodine receptor modulators          下载免费PDF全文

Mateus R Campos  Tadeu BM Silva  Wellington M Silva  Jefferson E Silva  Herbert AA Siqueira 《Pest management science》2015,71(4):537-544

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Predicting the number of nymphal instars plus new-generation adults of the Sunn Pest from overwintered adult densities and parasitism rates     

Halil Kutuk  Ramazan Canhilal  Mahmut Islamoglu  Ali Duran Kanat  Moustafa El-Bouhssini 《Journal of pest science》2010,83(1):21-25

The present study evaluated the effect of overwintered adult density and egg and adult parasitism rates on the new-generation population densities of the Sunn Pest (Eurygaster integriceps Put.) under field conditions. We quantified the abundance of overwintered adult densities and egg and adult parasitism rates in 20 one-ha unsprayed wheat fields in southeastern Turkey to assess their impact on nymphal instars, plus new-generation adult densities. The number of overwintered adults per square meter and the percent of egg parasitism rates were positively and negatively correlated with new-generation densities of the Sunn Pest, respectively. The results of multiple regression analysis produced the equation Y = 15.037 + 8.287 OW (overwintered adult) − 0.235 egg PR (parasitism rate). The findings suggest that the egg parasitoids may suppress nymphal instars plus new-generation adult densities of the Sunn Pest. The findings also suggest that the present formula can be used to determine the spraying areas in which densities reach economic threshold for the integrated Sunn Pest management program in wheat fields in Turkey.  相似文献   

Selenium Distribution for Soils Derived from Mancos Shale in Gunnison and Uncompahgre River Basins,West-Central Colorado     

Moustafa A. Elrashidi 《Communications in Soil Science and Plant Analysis》2018,49(9):1083-1091

Selenium (Se) is an essential micronutrient for humans and animals and can be toxic when present in high concentrations in soil and water. Many soils in Gunnison and Uncompahgre River Basins in West-Central Colorado are formed from deposits derived mainly from weathered Mancos shale that have high concentration of Se and salts. Elevated concentrations of Se and salts were detected in streams and rivers in this area. The objectives were to determine the amount and distribution of different forms of Se and relationships with physical and chemical properties for soils derived from Se-rich Mancos shale. The information may help to improve land management practices and minimize the environmental impact on natural water resources. A total of 48 topsoil samples and soils from horizons in 9 pedons were investigated. The total and water-soluble Se and other elements as well as physical and chemical properties were determined in soils. The total Se ranged from 330 to 5,673 µg/kg with a mean of 2,224 µg/kg. The water-soluble Se ranged from a minimum of 4.95 µg/kg to a maximum of 2,415 µg/kg and a mean of 338 µg/kg. A highly significant correlation was detected between the total and water-soluble Se in soils. Both the total organic carbon (TOC) and CaCO₃% had significant positive correlations with the total Se. A highly significant correlation was also found between the water-soluble Se and electric conductivity (EC), as well as with the water-soluble chloride, sulfate, nitrate, molybdenum, and sodium in soils. For the pedons tested, in general, both the total and water-soluble Se increased with depth. However, the water-soluble Se concentrations were greater in shallow than deep pedons where most Se was removed from soils with drainage water. It could be concluded that Se leaching from topsoils and that dissolving from Se-rich parent materials would be a major source of elevated Se concentrations in streams/rivers for the West-Central Colorado area. To minimize the impact on water quality, an appropriate land/water management practices should be followed to minimize Se leaching from soils derived from Mancos shale.  相似文献   

Carbon structures with three-dimensional periodicity at optical wavelengths   总被引：1，自引：0，他引：1  

AA Zakhidov  RH Baughman  Z Iqbal  C Cui  I Khayrullin  SO Dantas  J Marti  VG Ralchenko 《Science (New York, N.Y.)》1998,282(5390):897-901

Porous carbons that are three-dimensionally periodic on the scale of optical wavelengths were made by a synthesis route resembling the geological formation of natural opal. Porous silica opal crystals were sintered to form an intersphere interface through which the silica was removed after infiltration with carbon or a carbon precursor. The resulting porous carbons had different structures depending on synthesis conditions. Both diamond and glassy carbon inverse opals resulted from volume filling. Graphite inverse opals, comprising 40-angstrom-thick layers of graphite sheets tiled on spherical surfaces, were produced by surface templating. The carbon inverse opals provide examples of both dielectric and metallic optical photonic crystals. They strongly diffract light and may provide a route toward photonic band-gap materials.  相似文献   

Two Methods of Vitrification Followed by In Vitro Culture of the Ovine Ovary: Evaluation of the Follicular Development and Ovarian Extracellular Matrix          下载免费PDF全文

FT Bandeira  AA Carvalho  SV Castro  LF Lima  DA Viana  JSAM Evangelista  MJS Pereira  CC Campello  JR Figueiredo  APR Rodrigues 《Reproduction in domestic animals》2015,50(2):177-185

The aim of this study was to evaluate the influence of two vitrification techniques on the extra cellular matrix (ECM) and ovarian follicular development. The ovarian cortex was fragmented (9 mm³) and divided into six groups, viz. fresh control, cultured control, vitrified by the Ovarian Tissue Cryosystem (OTC) method, conventional solid surface vitrification (SSV) method, OTC/cultured and SSV/cultured. Follicles from all the fragments were analysed for morphology, development and viability. The ECM was evaluated based on the condition of collagen and reticular fibres and the immunolocalization of type I collagen and fibronectin. After 7 days of culture, the tissue vitrified by OTC revealed a higher percentage (p < 0.05) of morphologically normal (30.66%) and viable (60.00%) follicles when compared with those vitrified using the SSV technique (21.33% and 23.00%). In all the fragments cultured, regardless of the vitrification method, a significantly higher percentage of developing follicles was observed when compared with the non‐cultured tissue. Analysis of the type I collagen showed increased immunostaining after the in vitro culture in the vitrified fragments. In conclusion, the OTC is better for preserving the follicular viability and morphology and maintaining the integrity of the extracellular matrix components of the ovine ovary.  相似文献   

In vitro and in vivo toxicity of nano chitosan against Curvularia lunata,the causal microorganism of fruit rot and blight,a new disease of olive (O. europaea L.)     

Mohamed  Mazhar D. A.  Moharam  Moustafa H. A.  Ahmed  Hoda A. M. 《European journal of plant pathology / European Foundation for Plant Pathology》2021,161(4):881-894

During 2019, fruit blight and rot symptoms were observed on olive (O. europaea L.) fruits on trees grown in the Experimental Farm, Faculty of Agriculture, Sohag University, Egypt. Fungal isolates recovered from symptomatic fruits were identified as Curvularia lunata (Walker) Boedijn (two isolates) and A. alternata (Fr.) Keissl. (one isolate). Koch’s postulates were fulfilled by a pathogenicity test conducted in vitro on olive fruits wounded before inoculation with fungal isolates and incubation at 25?±?0.2 °C in a moist chamber for a week. During incubation, we observed the development of blight and rot symptoms on fruits inoculated with both isolates of C. lunata, similar to the natural symptoms described. Conversely, A. alternata was nonpathogenic to olive fruits. PCR amplification using the specific P1 and P2 primers to C. lunata based on the Clg2p Ras protein gene sequences resulted in approx. 870 base pairs for all DNA of C. lunata analyzed, confirming the identification of C. lunata. In vitro, both chitosan nano and non-nano scale effectively inhibited mycelial growth by reducing linear mycelium and biomass and sporulation of C. lunata. In vivo, chitosan nanoscale at 2.0 mg mL^?1 greatly reduced the infection and the lesion diameter of C. lunata inoculated fruits after a week and effectively induced defense-related enzyme activity of PO, PPO, and PAL. This report is the first recording of fruit blight and rots on olive caused by C. lunata, as a new disease. Also, we report the in vitro and vivo toxicity of nanoparticles of chitosan as a natural elicitor, effectively inducing defense-related enzymes against C. lunata.

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Eutrophication,Ammonia Intoxication,and Infectious Diseases: Interdisciplinary Factors of Mass Mortalities in Cultured Nile Tilapia     

Nermeen M. Abu-Elala  Reham M. Abd-Elsalam  Sherif Marouf  Mohamed Abdelaziz  Mohamed Moustafa 《Journal of aquatic animal health》2016,28(3):187-198

The present study was designed to assess the possible causes of the mass mortalities of Nile Tilapia Oreochromis niloticus at El-Behera Governorate, Egypt, in relationship to environmental and microbiotic factors. Water samples were collected from fish farms at different locations and from Lake Edku to analyze water temperature, water pH, salinity, biological oxygen demand, dissolved oxygen, total ammonia nitrogen, and un-ionized ammonia. A number of moribund and freshly dead fish were sampled and submitted to our laboratory for microbiological, molecular, and histopathological examination. Water analysis of the fish farms revealed noticeable increases in the previously mentioned physicochemical parameters. Clinical examinations of moribund fish showed severe gill rot and massive external and internal hemorrhages. Ordinary and molecular laboratory findings confirmed the presence of Branchiomyces sp. in gill tissue and mixed bacterial fish pathogens (Streptococcus agalactiae, Vibrio alginolyticus, V. parahaemolyticus, Pseudomonas anguilliseptica, and P. aeruginosa) in visceral organs. The histopathological and transmission electron microscopic examinations revealed severe necrosis of gill filaments and blockage of branchial blood vessels and lamellar capillaries with Branchiomyces sp. hyphae and spores mixed with different shapes of bacteria. Severe inflammations were detected in liver, kidney, heart, and brain tissues. Ultimately, we can conclude that the syndrome of mass fish kills in this area is a consequence of ecological damage to the aquatic environment, which is mainly related to natural and anthropogenic factors, as well as to the presence of infectious agents.

Received September 30, 2015; accepted April 12, 2016 Published online August 2, 2016  相似文献   

Detection and isolation of Toxoplasma gondii from fresh semen of naturally infected dogs in Southern Brazil          下载免费PDF全文

MO Koch  RR Weiss  AA Cruz  VT Soccol  KA Gonçalves  MAF Bertol  OC Beltrame  RL Dittrich 《Reproduction in domestic animals》2016,51(4):550-554

The aim of this study was to isolate Toxoplasma gondii and determine the viability of the parasite in fresh semen samples of clinically healthy adult dogs naturally infected. Eleven seropositive dogs with T. gondii IgG antibodies from southern Brazil were selected to confirm the presence and viability of T. gondii in fresh semen samples using in vitro isolation in Vero cell culture, polymerase chain reaction (PCR) and sequencing analysis. The presence of viable T. gondii was confirmed by in vitro isolation and PCR in five semen samples. The ITS1 region of the isolated protozoa (TG S4) was amplified and sequenced. The nucleotide sequence obtained was 99% compatible with the T. gondii DNA sequences stored in the GenBank. It has been shown that T. gondii tachyzoites may be isolated in vitro from fresh semen samples of clinically healthy dogs seropositive for T. gondii.  相似文献   

Changes in the Expression of the Prolactin Receptor (PRLR) Gene in Different Physiological Stages in the Mammary Gland of the Iranian Adani Goat          下载免费PDF全文

S Morammazi  AA Masoudi  R Vaez Torshizi  A Pakdel 《Reproduction in domestic animals》2016,51(4):585-590

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