Immunophenotype and functional properties of feline dendritic cells derived from blood and bone marrow     

Bienzle D  Reggeti F  Clark ME  Chow C 《Veterinary immunology and immunopathology》2003,96(1-2):19-30

Dendritic cells (DCs) are a heterogeneous population of cells of fundamental importance in initiating innate as well as specific immune responses. The identity and function of DCs in the cat are unknown, although they are likely pivotal in the response to infection. In this study, feline DCs were derived by 3-10-day culture of adherent blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) in the presence of IL 4 and GM-CSF. BMMC consistently yielded a greater number of DCs than PBMC, and there were fewer macrophages than DC from both compartments. DCs expressed a distinct constellation of surface molecules, which included CD1a, CD1b, and CD1c, CD11b, CD14, and 2-3-fold higher levels of MHC class I and II molecules than co-cultured macrophages or fresh blood monocytes. DCs displayed typical cytoplasmic processes, limited non-specific esterase activity, and acquired antigen by phagocytosis, pinocytosis, and binding to specific receptors. Cytokine-exposed cells induced proliferation of allogeneic lymphocytes. Thus, the cells derived by these culture conditions had markers and functions analogous to immature myeloid DCs. Availability of feline DCs will enable investigation of their role in infectious disease and their potential therapeutic application.  相似文献   

Temporal,environmental and management factors influencing the epidemiological patterns of sea lice (Lepeophtheirus salmonis) infestations on farmed Atlantic salmon (Salmo salar) in Scotland     

Revie CW  Gettinby G  Treasurer JW  Rae GH  Clark N 《Pest management science》2002,58(6):576-584

In association with industry, an extensive database has been established on measurements of sea louse counts on farmed Atlantic salmon for the years 1996 to 2000 from 33 Scottish fish farms. These data include extensive counts on the sea louse species, Lepeophtheirus salmonis, at various stages of the life cycle and in particular the chalimus and mobile stages. There has been considerable speculation as to what factors might affect the abundance of sea lice, much of which is based on limited evidence. Our analyses show that there is tremendous variation in sea louse infestation patterns from year to year, whereas stock type, geographical region and coastal exposure do not appear to affect mean levels of abundance. In contrast, treatments lead to pronounced cycles of sea louse infestation with peaks and troughs at 3-week intervals, and these interventions are important if the sea louse levels on fish are to be controlled. There was no evidence of water temperature affecting the mean annual abundance of sea louse infestation.  相似文献   

Unusual outbreak of sporozoan encephalomyelitis in Bluefaced Leicester ram lambs     

Sargison ND  Schock A  Maclean IA  Rae A  Heckeroth AR  Clark AM 《The Veterinary record》2000,146(8):225-226

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Minimally invasive approach to canine pericardiectomy     

Sobel DS  Clark GN 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2000,14(4):391

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Detection and quantification of equine herpesvirus-1 viremia and nasal shedding by real-time polymerase chain reaction.     

Stephen B Hussey  Rodney Clark  Katharine F Lunn  Cormac Breathnach  Gisela Soboll  J Millar Whalley  D Paul Lunn 《Journal of veterinary diagnostic investigation》2006,18(4):335-342

Equine herpesvirus-1 (EHV-1) infection is common in young horses throughout the world, resulting in respiratory disease, epidemic abortion, sporadic myelitis, or latent infections. To improve on conventional diagnostic tests for EHV-1, a real-time polymerase chain reaction (PCR) technique was developed, using primers and probes specific for the EHV-1 gB gene. Amplification efficiencies of 100% +/- 5% were obtained for DNA isolated from a plasmid, infected peripheral blood mononuclear cells (PBMCs), and nasal secretions from infected ponies. The dynamic range of the assay was 8 log10 dilutions, and the lower limit of detection was 6 DNA copies. Fifteen ponies, seronegative for EHV-1, were experimentally infected with EHV-1, and nasal samples were used to quantify shedding of virus by both virus isolation and real-time PCR analysis. Virus isolation identified nasal shedding of EHV-1 in 12/15 ponies on a total of 25 days; real-time PCR detected viral shedding in 15/15 ponies on 75 days. Viremia was quantified using PBMC DNA, subsequent to challenge infection in 3 additional ponies. Viremia was identified in 1/3 ponies on a single day by virus isolation; real-time PCR detected viremia in 3/3 ponies on 17 days. When real-time PCR was used to analyze PBMC DNA from 11 latently infected ponies (documented by nested PCR), EHV-1 was not detected. We conclude that real-time PCR is a sensitive and quantitative test for EHV-1 nasal shedding and viremia and provides a valuable tool for EHV-1 surveillance, diagnosis of clinical disease, and investigation of vaccine efficacy.  相似文献   

反刍动物甲烷生成量的调控     

张晓庆  金艳梅  那日苏 《家畜生态》2006,(6)

反刍动物体内甲烷的产生是瘤胃发酵能损失的主要原因,减少甲烷产量对提高反刍动物的能量利用率和环境保护均具有重要意义。因此,本文就反刍动物甲烷生成的机制及影响其生成量的因素等方面做一综述,并对降低甲烷生成量的调控方式进行了分析和展望。  相似文献   

饲用莫能菌素的研究进展   总被引：2，自引：0，他引：2  

张晓庆  那日苏  周淑清 《中国牛业科学》2006,33(3):44-46

莫能菌素是聚醚类离子载体抗生素,是目前欧盟唯一允许使用的抗生素饲料添加剂。本文通过其作用机理、畜禽生产中的添加效果及使用剂量等方面的综合论述,证明了在畜禽生产中饲用莫能菌素的可行性,并为其今后的广泛应用做了研究展望。  相似文献   

精子受精抗原-1(FA-1) mRNA在绵羊睾丸和附睾中的表达   总被引：1，自引：0，他引：1  

娜仁花  旭日干 《畜牧与兽医》2006,38(11):1-3

本研究通过RT-PCR检测了该基因在绵羊睾丸和附睾中的表达情况。首先,提取绵羊睾丸组织、附睾头、体、尾部组织总RNA,以此为模板,反转录合成cDNA,自行设计引物,PCR扩增出462 bp的目的DNA。然后,将目的片断克隆入T载体,通过菌液PCR和重组质粒酶切,鉴定重组质粒中的目的DNA。再经序列分析鉴定目的片断。同时,以β-actin为内参照物,进行RT-PCR半定量分析,比较精子受精抗原(FA-1)在睾丸、附睾头、体、尾组织中的表达量。结果表明,FA-1在绵羊睾丸和附睾中均表达。  相似文献   

日光温室牡丹花石榴光合特性研究   总被引：1，自引：0，他引：1  

王彪郭修武  蒋锦标王国栋 那颖 《北方园艺》2007,(6):96-98

以LI-6400光合作用测定系统对日光温室牡丹花石榴的光合特性进行测定。结果表明,在晴天条件下,牡丹花石榴光合速率日变化呈双峰曲线,最高峰在8时,次峰在15时,中午有明显的“午休”现象。光合作用的光补偿点和饱和点分别为32μmol/m2/s和1200μmol/m2/s,CO2补偿点和饱和点分别为16μmol/mol和850μmol/mol。  相似文献   

Red clover mottle virus from Ukraine is an isolate of RCMV strain S     

L.G. Lapchic  A.J. Clark  G.P. Lomonossoff  M. Shanks 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(4):409-412

The relationship between red clover mottle virus (RCMV) isolated in the Ukraine (designated RCMV-Uk) and well-characterised strains from Sweden has been investigated. Nucleic acid hybridisation indicate that both RNAs from RCMV-Uk are highly homologous to their counterparts from RCMV strain S, a conclusion supported by protein sequence analysis of the two viral capsid proteins. Nucleic acid sequence analysis of a portion of RCMV-Uk RNA2 confirmed the high degree of similarity between RCMV-Uk and RCMV strain S. This information suggests that RCMV-Uk should be considered an isolate of RCMV strain S.  相似文献