Ovarian Responses, Hormonal Profiles and Embryo Yields in Anoestrous Ewes Superovulated with Folltropin®-V after Pretreatment with Medroxyprogesterone Acetate-releasing Vaginal Sponges and a Single Dose of Oestradiol-17β     

PM Bartlewski  BD Alexander  NC Rawlings  DMW Barrett  WA King 《Reproduction in domestic animals》2008,43(3):299-307

In ruminants, superovulatory treatments started at the time of follicular wave emergence result in greater and less variable ovulatory responses and embryo yields compared with the treatments begun in the presence of a large growing antral follicle(s) from the previous waves. The progesterone–oestradiol treatment is routinely used for follicular wave synchronization in cattle. The main objective of this study was to characterize the ovarian responses, hormonal profiles and in vivo embryo production in anoestrous Rideau Arcott ewes (May‐June), which were superovulated after pretreatment with medroxyprogesterone acetate (MAP)‐releasing intravaginal sponges and a single dose of oestradiol‐17β (E₂‐17β). Six days after insertion of MAP sponges, eight ewes were given an i.m. injection of 350 μg of E₂‐17β (E₂‐17β‐treated ewes); 10 ewes were given an i.m. injection of vehicle (control ewes). Multiple‐dose Folltropin®‐V treatment, followed by the bolus injection of GnRH (50 μg i.m.), began 6 days after E₂‐17β/vehicle injection. Transrectal ovarian ultrasonography revealed that: (i) the interval between E₂‐17β/vehicle injection and regression of all follicles ≥5 to 3 mm in diameter was shorter (p < 0.01; 2.6 ± 0.4 vs 4.8 ± 0.6 days respectively); and (ii) the interval between injection and emergence of the next follicular wave was longer (p < 0.05; 5.4 ± 0.3 vs 1.2 ± 0.4 days, respectively) in E₂‐17β‐treated than in control ewes. During the 6 days after injection, the mean FSH peak concentration and basal FSH concentration were lower (p < 0.01) in E₂‐17β‐treated ewes. The mean ovulation rate and the number of recovered embryos did not differ (p > 0.05) between the two groups of ewes. However, the number of luteinized unovulated follicles per ewe, and the variability in the number of luteal structures and overall embryo yield were less (p < 0.05) in E₂‐17β‐treated compared with control ewes. In conclusion, the MAP–E₂‐17β pretreatment significantly reduced the variability in ovarian responses and embryo yields, without affecting the embryo production in superovulated anoestrous ewes.  相似文献   

Rethinking biorational insecticides for pest management: unintended effects and consequences     

Khalid Haddi  Leonardo M Turchen  Luis O Viteri Jumbo  Raul NC Guedes  Eliseu JG Pereira  Raimundo WS Aguiar  Eugênio E Oliveira 《Pest management science》2020,76(7):2286-2293

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A Universal Index of Uterine Discharge Symptoms from Calving to 6 Weeks Postpartum     

J Gorzecka  NC Friggens  C Ridder  H Callesen 《Reproduction in domestic animals》2011,46(1):100-107

In this paper, a new uterine discharge index (D‐index) was created and tested. It was based on a principal component analysis (PCA) of clinical findings of classical uterine discharge symptoms and rectal temperature during the postpartum period of dairy cattle. The PCA analysis revealed how uterine discharge features relate to each other and how they cluster together possibly representing different degrees of uterine inflammation. The D‐index was the result of the multivariate PCA‐analysis, and the D‐index gives a continuous value between 0 and 10. It was demonstrated that the same scale, i.e. the D‐index, can be used without any adjustment from 1 to 6 weeks post‐calving. It is valid for any type of uterine discharge without defining the type of infection or differentiating between infection and contamination. The D‐index was tested using the uterine involution data. Uterine involution was significantly delayed in the high‐D‐index group of cows. Similarly, in the test with all cows, involution was progressively delayed and the rate of involution of the pregnant horn was slowed down with the increase of the D‐index values. It is concluded that the D‐index can be a new practical, universal, tool for improved management of dairy cows in the postpartum period under commercial conditions.  相似文献   

Imaging of Plasmodium liver stages to drive next-generation antimalarial drug discovery     

Meister S  Plouffe DM  Kuhen KL  Bonamy GM  Wu T  Barnes SW  Bopp SE  Borboa R  Bright AT  Che J  Cohen S  Dharia NV  Gagaring K  Gettayacamin M  Gordon P  Groessl T  Kato N  Lee MC  McNamara CW  Fidock DA  Nagle A  Nam TG  Richmond W  Roland J  Rottmann M  Zhou B  Froissard P  Glynne RJ  Mazier D  Sattabongkot J  Schultz PG  Tuntland T  Walker JR  Zhou Y  Chatterjee A  Diagana TT  Winzeler EA 《Science (New York, N.Y.)》2011,334(6061):1372-1377

Most malaria drug development focuses on parasite stages detected in red blood cells, even though, to achieve eradication, next-generation drugs active against both erythrocytic and exo-erythrocytic forms would be preferable. We applied a multifactorial approach to a set of >4000 commercially available compounds with previously demonstrated blood-stage activity (median inhibitory concentration < 1 micromolar) and identified chemical scaffolds with potent activity against both forms. From this screen, we identified an imidazolopiperazine scaffold series that was highly enriched among compounds active against Plasmodium liver stages. The orally bioavailable lead imidazolopiperazine confers complete causal prophylactic protection (15 milligrams/kilogram) in rodent models of malaria and shows potent in vivo blood-stage therapeutic activity. The open-source chemical tools resulting from our effort provide starting points for future drug discovery programs, as well as opportunities for researchers to investigate the biology of exo-erythrocytic forms.  相似文献   

A DIAPORTHE CANKER OF AMERICAN ELM     

Richmond BG 《Science (New York, N.Y.)》1932,75(1934):110-111

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Health insurance in the USA     

Richmond JB  Fein R 《Science (New York, N.Y.)》2003,301(5641):1813

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FACILITATE HUMAN ENDEAVOR THROUGH COLLEGE TRAINING IN SCIENTIFIC METHOD     

Richmond KC 《Science (New York, N.Y.)》1944,99(2576):385-386

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Concurrent processing and complexity of temporally encoded neuronal messages in visual perception   总被引：2，自引：0，他引：2  

J W McClurkin  L M Optican  B J Richmond  T J Gawne 《Science (New York, N.Y.)》1991,253(5020):675-677

The intrinsic neuronal code that carries visual information and the perceptual mechanism for decoding that information are not known. However, multivariate statistics and information theory show that neurons in four visual areas simultaneously carry multiple, stimulus-related messages by utilizing multiplexed temporal codes. The complexity of these temporal messages increases progressively across the visual system, yet the temporal codes overlap in time. Thus, visual perception may depend on the concurrent processing of multiplexed temporal messages from all visual areas.  相似文献   

Interferon inducers and foot-and-mouth disease vaccines: influence of two synthetic polynucleotides on antibody response and immunity in guinea pigs and swine.          下载免费PDF全文

H R Cunliffe  J Y Richmond    C H Campbell 《Canadian journal of veterinary research》1977,41(1):117-121

Polyriboadenylic-polybouridylic acid enhanced the immunological response of guinea pigs to aqueous foot-and-mouth disease virus vaccine. Polyriboninosinic-polyribocytidylic acid enhanced the early antibody production of swine to oil emulsified foot-and-mouth disease virus vaccine. Polyriboninosinic-polyribocytidylic acid alone did not stimulate resistance to foot-and-mouth disease in swine.  相似文献   

From the genome to the proteome: uncovering peptides in the Apis brain     

Hummon AB  Richmond TA  Verleyen P  Baggerman G  Huybrechts J  Ewing MA  Vierstraete E  Rodriguez-Zas SL  Schoofs L  Robinson GE  Sweedler JV 《Science (New York, N.Y.)》2006,314(5799):647-649

Neuropeptides, critical brain peptides that modulate animal behavior by affecting the activity of almost every neuronal circuit, are inherently difficult to predict directly from a nascent genome sequence because of extensive posttranslational processing. The combination of bioinformatics and proteomics allows unprecedented neuropeptide discovery from an unannotated genome. Within the Apis mellifera genome, we have inferred more than 200 neuropeptides and have confirmed the sequences of 100 peptides. This study lays the groundwork for future molecular studies of Apis neuropeptides with the identification of 36 genes, 33 of which were previously unreported.  相似文献