Study on Anti-viral Effect of Rhubarb on Porcine Epidemic Diarrhea Virus in vitro     

CHEN Tian-tian  CHENG Fu-liang  ZHANG Jian-mei  NIE Zhao-jing  FANG Dong  FAN Mei-na  LI Fu-hui  CHENG He-gang 《中国畜牧兽医》2015,42(9):2474-2480

The inhibitory effects of rhubarb on cell lesion, which was caused by porcine epidemic diarrhea virus (PEDV), and its mechanism had been studied in this research.The maximum safe concentration of rhubarb solution applied to Vero cells was determined through morphological observation, and then the PEDV infected Vero cell model in vitro was established, by which the inhibitory effect of rhubarb on virus was studied.The experimental method had also been established based on this cell model.The result showed that the maximum safe concentration was 3.28 mg/mL.Both morphological observation and MTT assay were indicated that rhubarb could not only block the attachment of the virus to Vero cell and inhibit the synthesis and reproduction of the virus, but also could directly inactivate the virus.This research showed that rhubarb had an inhibitory effect on PEDV, and provided a theoretical basis for the prevention and treatment of clinical porcine epidemic diarrhea.  相似文献   

港口原油装船VOCs大气环境影响探讨   总被引：1，自引：0，他引：1  

李鑫  寿幼平 《绿色科技》2015,(3):181-182

收集了原油装船VOCs的估算方法，认为原油装船时VOCs排放量为0.012％，VOCs浓度为0.1kg／m3。采用SCREEN3对其环境影响进行分了析，结果表明：下风向最大浓度出现在400m 处，浓度值7.99mg／m3，直到2700m处，浓度值才小于1mg／m3，直到10000m处，浓度值仍为0.14mg／m3，原油装船VOCs的大气环境影响较大。  相似文献   

基于MMC的RPC环流抑制策略     

杨欣 《湖南农业大学学报(自然科学版)》2016,(4):15-21

RPC能够对电气化铁路中产生的负序和无功进行就地综合补偿，适用于对高速铁路突出的负序和谐波问题进行综合治理，但RPC受到器件性能的限制难以增大其容量从而影响RPC未来的发展。针对这一问题，有学者提出基于单相MMC的RPC综合补偿装置（MRPC），MRPC提高了应用的电压等级，有利于适应高压大功率场合。但MRPC中的环流增大了系统损耗，影响了系统稳定，严重时可能导致系统崩溃。针对这一问题，本文详细推导了MRPC的数学模型；分析了环流形成的机理；最后提出了一种环流抑制策略。仿真结果表明，所提出的控制策略可以抑制MRPC中的环流改善其的控制性能，有利于MRPC的实际应用。  相似文献   

基于遗传算法的PVC企业生产计划的模糊优化     

王心 《中国农机化》2011,(5)

目前国内的PVC市场已经产能过剩,PVC企业只有利用产业链扩充,才能增强竞争力.投资生产PVC下游产品,必然涉及到生产计划问题.在复杂的市场环境下,许多数据的来源是不精确的,因此,本文应用模糊理论,建立了PVC下游产品生产计划的模糊期望目标规划模型,并设计了一种基于模糊模拟的遗传算法对模型进行求解,最后通过实例分析证明该方法的可行性和有效性.该方法不仅能够降低企业获取信息的成本,更能真实的反映实际情况,对我国PVC企业的发展具有现实意义.  相似文献   

不同形态外源氮对马尾松与广玉兰落叶分解的影响   总被引：1，自引：0，他引：1  

周林涛  黄欣  宋曰钦 《湖南林业科技》2017,44(2)

以马尾松及广玉兰落叶为试验材料,以浓度均为75 mg/kg的NH_4Cl、KNO_3、NH4NO_3溶液为试验处理液,采用分解袋方法,通过对落叶施加不同形态外源氮的方式模拟氮沉降,研究不同形态外源氮对马尾松及广玉兰落叶分解的影响。结果表明:施加外源氮素可以促进落叶的分解,外源氮对不同树种落叶分解的促进效果不同;广玉兰落叶在外源氮处理后分解率增加更为明显,161天后其各处理分解率均在马尾松落叶分解率的1.2倍以上。氮源形态对马尾松、广玉兰落叶分解率的影响均达极显著(马尾松F=1 262.9 P0.01,广玉兰F=165.3 P0.01),其中硝态氮处理效果最好,161天后马尾松和广玉兰落叶的分解率分别为9.79%和13.02%。分解时间对马尾松与广玉兰落叶分解率的影响均达极显著。  相似文献   

基于LM算法的BP神经网络的电力负荷短期预测     

刘进波  陈鑫  李新花 《湖南农业大学学报(自然科学版)》2015,(2):34-38

清代书院课艺总集多为连续出版物，或具有连续出版物的刊行初衷。刊期短则一季，多则一年或数年。经费充足与否，会影响刊期。发表周期多为一年至五年，也有十余年的。用稿率以10%~20%居多，偶见“关系稿”。时文的用稿标准是“清真雅正”。题目多为官师所拟。一般全文刊登，也偶有“论点摘编”。多经润色，并附录评点。有的以袖珍本刊行，有的宣称“翻刻必究”，标出定价，附载广告。稿费已在膏火费中预支，优秀作品可被转载。从本质属性和诸多要素来看，书院课艺总集实开今日“大学学报”、“学术集刊”之先河。  相似文献   

激素配比和pH值对蓝莓试管苗增殖生长的影响     

张舵  杨艳敏  魏永祥  刘成  王莉  魏鑫  刘有春 《北方果树》2015,(3):13-14

该文以对土壤p H值适应性截然不同的蓝莓品种‘斯巴坦’和‘北陆’试管苗为试材,研究二者组织培养条件下对激素配比和p H值的要求。结果表明:‘斯巴坦’试管苗增殖培养基最佳激素配比为ZT0.3 mg/L+IBA 0.2 mg/L,p H值4.5;‘北陆’试管苗增殖培养基最佳激素配比ZT 0.3 mg/L+IBA 0.1 mg/L,p H值5.0。  相似文献   

维药菊苣提取物对Balb/c小鼠肝纤维化的治疗作用研究          下载免费PDF全文

向莉琳  李巍  武欣  王新春  简炎林 《勤云标准版测试》2015,35(8)

目的 探讨维药菊苣正丁醇提取物（CGB）抗雄性小鼠肝纤维化的治疗作用。方法 采用刀豆凝集素（Con A）在Balb/c雄性小鼠诱导肝纤维化，造模成功后第6周分别用低剂量（100 mg/kg）和高剂量（500 mg/kg）CGB干预治疗，并以秋水仙碱（0.1 mg/kg）做阳性对照。治疗第10周处死小鼠留取肝脏组织和血清，比较各组小鼠肝功能、关键性肝纤维化指标。病理分析肝脏组织变化和TGF-β1/Smad信号通路相关因子表达差异。结果 与模型组比较，CGB治疗组的肝功能指标（ALT，AST）和血清肝纤四项指标（PC-Ⅲ、CIV、LN和HA）指标不同程度降低（P＜0.05）；肝组织TGF-β1蛋白表达下降，Smad7蛋白表达升高（P＜0.05）。肝脏组织病理显示，不同剂量CGB治疗均能一定程度上减缓小鼠肝纤维化的发展。结论 CGB具有抗肝纤维化的活性，影响TGF-β1/Smad信号通路可能是其潜在的抗肝纤维化作用机制。  相似文献   

Effect of Alfalfa Flavonoids on Growth Performance and Serum Indexes of Sheep     

WANG Meng-zhu  LIU Yan-feng  WANG Wen-qi  NIE Cun-xi  TANG Shu-zhen  ZHANG Wen-ju 《中国畜牧兽医》2015,42(9):2345-2351

This experiment was conducted to evaluate the effect of alfalfa flavonoids (Medicago sativa) on growth performance and serum indexes of sheep.This experiment was performed with hybrid F1 sheep of Suffolk sheep×Small-tailed Han sheep.Twenty-eight sheep with the body weight of (27.02±3.03) kg were randomly divided into 4 groups with 7 replicates in each group and 1 sheep per replicate.Sheep in different groups were fed diets with 0 (group Ⅰ), 0.1% (group Ⅱ), 0.2%(group Ⅲ) and 0.4% (group Ⅳ) alfalfa flavonoids, respectively.The experiment was cosisted of 7-day-preliminary trial and 41-day-formal trial.The results showed as follows:① Dietary supplementation of alfalfa flavonoids had no significant effects on growth performance of sheep(P>0.05).② Dietary supplementation of alfalfa flavonoids had significant effects on the contents of GLB, ASP, BUN, HDL and P in serum (P<0.05).Serum GLB content at the 1st day in group Ⅲ was significantly lower than that in groupsⅠand Ⅱ(P<0.05), serum ASP contents at the 1st and 21th days in group Ⅳ were significantly lower than those in group Ⅰ(P<0.05), serum BUN content at the 1st day in group Ⅳ was significantly lower than that in group Ⅰ(P<0.05), serum HDL content at the 21th day in group Ⅱ was significantly lower than that in groups Ⅰ and Ⅲ (P<0.05), serum P content in group Ⅳ at the 21th day was significantly lower than that in groups Ⅱ and Ⅲ (P<0.05).In conclusion, dietary supplementation of alfalfa flavonoids might modulate lipid metabolism, improve nitrogen utilization, maintain energy metabolic and balance Ca and P contents, and have the potential function for promoting growth and no adverse effect on liver.The results suggested that the appropriate supplemental level of alfalfa flavonoids for sheep was 0.2%.  相似文献   

信息与计算科学专业《数据结构》教学实践与思考     

王鑫 《农业网络信息》2014,(5):134-136

结合信息与计算科学专业设置,本文分析了数据结构课程教学现状,从整合教学内容、设置教学梯度和完善教学手段等方面进行研究与探索。本课题针对交叉学科的特点强调学以致用和知识的融会贯通,搭建了开放的专题学习平台,提高了学生的自主学习和研究能力,初步取得了较好的教学效果,为数据结构课程的教学改革提供了参考。  相似文献