A comparison of whole-genome shotgun-derived mouse chromosome 16 and the human genome   总被引：1，自引：0，他引：1  

Mural RJ  Adams MD  Myers EW  Smith HO  Miklos GL  Wides R  Halpern A  Li PW  Sutton GG  Nadeau J  Salzberg SL  Holt RA  Kodira CD  Lu F  Chen L  Deng Z  Evangelista CC  Gan W  Heiman TJ  Li J  Li Z  Merkulov GV  Milshina NV  Naik AK  Qi R  Shue BC  Wang A  Wang J  Wang X  Yan X  Ye J  Yooseph S  Zhao Q  Zheng L  Zhu SC  Biddick K  Bolanos R  Delcher AL  Dew IM  Fasulo D  Flanigan MJ  Huson DH  Kravitz SA  Miller JR  Mobarry CM  Reinert K  Remington KA  Zhang Q  Zheng XH  Nusskern DR  Lai Z  Lei Y  Zhong W  Yao A  Guan P  Ji RR  Gu Z  Wang ZY  Zhong F 《Science (New York, N.Y.)》2002,296(5573):1661-1671

The high degree of similarity between the mouse and human genomes is demonstrated through analysis of the sequence of mouse chromosome 16 (Mmu 16), which was obtained as part of a whole-genome shotgun assembly of the mouse genome. The mouse genome is about 10% smaller than the human genome, owing to a lower repetitive DNA content. Comparison of the structure and protein-coding potential of Mmu 16 with that of the homologous segments of the human genome identifies regions of conserved synteny with human chromosomes (Hsa) 3, 8, 12, 16, 21, and 22. Gene content and order are highly conserved between Mmu 16 and the syntenic blocks of the human genome. Of the 731 predicted genes on Mmu 16, 509 align with orthologs on the corresponding portions of the human genome, 44 are likely paralogous to these genes, and 164 genes have homologs elsewhere in the human genome; there are 14 genes for which we could find no human counterpart.  相似文献   

Genetics. Modifying the message     

Nadeau JH 《Science (New York, N.Y.)》2003,301(5635):927-928

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Genetics. Systems genetics     

Nadeau JH  Dudley AM 《Science (New York, N.Y.)》2011,331(6020):1015-1016

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Continuous infusion of propofol in dogs premedicated with methotrimeprazine   总被引：1，自引：0，他引：1  

Antonio JA Aguiar p  hd Stelio PL Luna p  hd Valéria NLS Oliva p  hd  Flávia R Eugênio MSc  Gladys B Castro p  hd 《Veterinary anaesthesia and analgesia》2001,28(4):220-224

Objective To evaluate the cardiopulmonary and clinical effects of three different infusion rates of propofol in dogs premedicated with methotrimeprazine. Study design Randomized experimental trial. Animals Ten healthy adult mixed‐breed male and female dogs, weighing from 14 to 20 kg. Methods Dogs were premedicated with methotrimeprazine [1 mg kg^?1 intravenously (IV)] followed by induction of anesthesia with 4.5 mg kg^?1 of propofol IV and maintenance with propofol for 60 minutes as follows: T1, 0.2 mg kg^?1 minute^?1; T2, 0.3 mg kg^?1minute^?1; and T3, 0.4 mg kg^?1minute^?1. Heart rate (HR), respiratory rate (RR), mean arterial pressure (MAP), end‐tidal CO₂ (P_ETCO₂), arterial hemoglobin O₂ saturation, arterial blood gases, and pedal and cutaneous reflexes were measured before and 5, 10, 20, 30, 45 and 60 minutes after the beginning of the propofol infusion. Statistical analysis was performed using an anova . Results Heart rate increased during anesthesia in all cases and arterial blood pressure decreased only in dogs in the T3 category. Respiratory depression was proportional to the infusion rate of propofol. Muscle relaxation was satisfactory, but analgesia was inadequate in the three treatments. Conclusions The infusion of 0.2–0.4 mg kg^?1 minute^?1 of propofol produced a dose‐dependent respiratory depression. The presence of a pedal withdrawal reflex and marked cardiovascular responses to this noxious stimulus suggests that anesthesia may not be of sufficient depth for surgery to be carried out. Clinical relevance Although several studies have been performed using propofol in animals, few studies have investigated the cardiopulmonary and analgesic effects with different doses. The determination of an adequate propofol infusion rate is necessary for the routine use of this intravenous anesthetic for the maintenance of anesthesia during major surgical procedures in dogs.  相似文献   

Inhalant anesthetic requirement in cats is animal-dependent     

LS  Barter  JE Ilkiw  EP Steffey  BH Pypendop  A Imai  JA Bolich 《Veterinary anaesthesia and analgesia》2003,30(2):105-105

Minimum alveolar concentration (MAC) of an inhalant is an indicator of its anesthetic potency. Individuals vary in their sensitivity to anesthetic agents as demonstrated by different individual MAC values. We hypothesized that individual animal sensitivity would be maintained with different inhalant anesthetics. As part of separate studies, six female DSH cats, aged 24 ± 2.5 (mean ± SD) months and weighing 3.5 ± 0.3 kg, were studied similarly on three separate occasions over a 12‐month period to determine the MAC of isoflurane (ISO), sevoflurane (SEVO), and desflurane (DES), respectively. In each study, chamber induction was followed by orotracheal intubation, and anesthesia was maintained via a nonrebreathing circuit. ECG, pulse oximetry, Doppler systolic blood pressure, end‐tidal gases, and esophageal temperature were monitored. End‐tidal gases were hand‐sampled from a catheter whose tip lay level with the distal end of the ET tube. Gases were analyzed by Raman spectrometry and, for each agent, the analyzer was calibrated with at least three gas standards. MAC was determined in triplicate using standard tail‐clamp technique. Data were analyzed by two‐way anova followed by Tukey's test and significant differences were found. Average MACs (%) for ISO, SEVO, and DES were 1.90 ± 0.18, 3.41 ± 0.65, and 10.27 ± 1.06, respectively. Body temperatures, Doppler systolic blood pressure, and SpO₂ were recorded at the time of MAC determinations for ISO, SEVO, and DES were 38.3 ± 0.3, 38.6 ± 0.1, 38.3 ± 0.35 °C; 71 ± 8, 75 ± 16, 88 ± 12 mm Hg; 99 ± 1, 99 ± 1, 99 ± 1%, respectively. Both the anesthetic agent and the individual cat had significant effects on MAC (p = 0.0001 and 0.0185, respectively). MAC varied between individuals and cats were consistent in their order of sensitivity to inhalant anesthetics across the three agents. Within this group of cats, the relationship of individual MAC to the group MAC for each of the three inhalant agents was maintained. This suggests that any individual may be consistently more or less sensitive to a variety of inhalant agents.  相似文献   

P450‐aromatase mRNA is Expressed in the Corpus Luteum (CL) of the Non‐Pregnant Sheep and Goat: The Expression of the Enzyme is Present Throughout Pregnancy in the Goat CL     

JA Mondragón  C Miranda  J García‐Mena  R Ocádiz‐Delgado  P Gariglio  MC Romano 《Reproduction in domestic animals》2013,48(1):85-89

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Abortions in sheep caused by Salmonella Brandenburg: Pathological findings     

RG Clark  JM Gill  RA Fairley  JA Smart 《New Zealand veterinary journal》2013,61(6):356-357

CASE HISTORY: A 7-year-old cat developed sporadic vomiting, reduced appetite, and weight loss over the previous 3 months.

CLINICAL FINDINGS: Palpation revealed a large mid-abdominal mass and the cat had marked eosinophilia. The cat progressively lost weight over the next 7 weeks when euthanasia was performed.

PATHOLOGICAL FINDINGS: Necropsy revealed a 3?cm diameter firm white intramural mass in the colon and another in the pylorus. Mesenteric and cranial mediastinal lymph nodes were firm, pale, and enlarged. Histopathological examination revealed foci of necrosis surrounded by thick dense collagen trabeculae and predominantly eosinophilic inflammation within the intestine and lymph nodes. Marked eosinophilic infiltration of the liver was also present.

DIAGNOSIS: The lesions were consistent with gastrointestinal eosinophilic sclerosing fibroplasia (FGESF).

CLINICAL RELEVANCE: This is the first report of FGESF in a New Zealand cat and the first time lesions of FGESF have been observed in extra-abdominal tissues. Intestinal neoplasia can be clinically identical to FGESF and histopathology is required for differentiation. Evidence suggests that FGESF has a more favourable prognosis than intestinal neoplasia.  相似文献   

Confirmation of canine leproid granuloma syndrome in New Zealand     

B Smits  R Willis  JA Fyfe 《New Zealand veterinary journal》2013,61(3)

Canine leproid granuloma syndrome (CLGS) has not been officially reported in New Zealand. The seminal report describing this syndrome is in the Australian Veterinary Journal, 1998, where the results of a questionnaire circulated amongst veterinary pathologists and practitioners in Australia were reported. It included one response of a case seen in New Zealand, but no details of that case were given, despite CLGS being described in the literature as “common in New Zealand”. By injudicious use of references, the international literature has propagated the idea that the condition, including molecular identification, was confirmed in New Zealand, yet none of the articles cited actually confirmed that. An outbreak of skin granulomas in a group of approximately 35 working dogs was investigated, in which skin samples were sent to the Mycobacterium reference laboratory, Victoria, Australia, for PCR testing and molecular characterisation. Results of the clinical presentation, histological features and molecular studies conformed to the published details of CLGS. In particular, the nucleotide sequence of the internal transcribed spacer region, amplified from the mycobacterial DNA present in the clinical specimen provided, was identical to GenBank® Accession Number EF611177. That sequence is representative of the causative agent of CLGS in cases from Australia, the United States of America and Brazil. Although acid-fast organisms are occasionally seen in skin granulomas in dogs in New Zealand, this is the first confirmed identification of CLGS in this country. This is also the first report of an outbreak situation amongst a group of dogs.  相似文献   

Metastatic malignant pilomatrixoma,acanthomatous ameloblastoma,and liver tumor in a dog with polyphagia,polyuria, polydipsia,and weight loss     

Anne-Charlotte Barrot  Lisa Carioto  Malcolm Gains  Marie-Eve Nadeau 《The Canadian veterinary journal. La revue veterinaire canadienne》2013,54(4):387-391

A 12-year-old, spayed female, Labrador dog was presented for evaluation of polyphagia, polyuria, polydipsia, weight loss of 2 months duration, and multiple cutaneous and subcutaneous masses. The dog was diagnosed with malignant pilomatrixoma with renal, lung, and lumbar metastases. This report describes an atypical presentation of malignant pilomatrixoma.  相似文献   

Biometrics,Testosterone, Cortisol and Antler Growth Cycle in Iberian Red Deer Stags (Cervus elaphus hispanicus)     

E Gaspar‐López  T Landete‐Castillejos  JA Estevez  F Ceacero  L Gallego  AJ García 《Reproduction in domestic animals》2010,45(2):243-249

In this article, we aimed to describe the changes related to mating season in red deer, especially those related to antler growth, body condition score, testosterone and cortisol. Antler growth was studied in 17 Iberian red deer males, including body weight, antler length, biometric measures and testosterone and cortisol determination during 15 months. Body weight, body condition score, thoracic perimeter (TP), neck perimeter (NL) and testicular diameter (TD) showed the highest values immediately before mating season (autumn), decreased during it and remained constant at winter. Antler growth lasted 158 days and produced antlers with a final length of 80.8 ± 2.0 cm. Testosterone and cortisol showed seasonal changes with maximum values at September and May, respectively. Final antler size was related positively to cranial longitude, TP, NL, TD and body weight at casting time. No relationship between weight loss during precedent mating season and current antler size was found, but spring recovery weight was positively related to final antler size. Final length was related to the descent in testosterone values during previous mating season and to body weight before it. Spring recovery weight was related to relative weight loss during previous mating season. These results suggest that there is no relationship between the reproductive effort performed during one season and the next year size of the antler. In contrast, antler size was positively related to spring recovery weight, in the sense that those deer that recover a higher percentage of body weight at the early stages of antler growth develop higher antlers.  相似文献