大果沙棘全光照喷雾嫩枝扦插育苗试验   总被引：6，自引：0，他引：6  

撒文清  赵忠  张博勇  刘杜玲  张晓鹏 《西北林学院学报》2007,22(2):82-84,126

在全光照自动喷雾条件下,对大果沙棘嫩枝插条采用IBA、NAA、ABT-l号、ABT-2号4种植物生长调节剂,各3种浓度梯度的浸泡处理2 h,可明显促进插条生根。在供试的13种处理中,IBA 100 m g.L-1处理效果最好,生根率可达到98.8%。  相似文献   

水泡性口炎病毒核蛋白基因在大肠杆菌中的表达和鉴定     

盛祖勋  赵永刚  陈义平  萨仁高娃  王宝安  王志亮 《扬州大学学报(农业与生命科学版)》2005,26(4):5-7

根据已经发表的IN型水泡性口炎病毒(VSV)核蛋白(N)基因的序列设计1对特异引物,应用RT-PCR技术扩增编码IN VSV的N基因,将PCR产物按相应的阅读框架克隆到表达载体pET-32a。并将重组质粒转化入大肠杆菌 BL21株,以1．0 mmol·L-1 IPTG在37℃的条件下诱导,N基因得到了高效表达。经过聚丙烯酰胺凝胶电泳以及用 VSV阳性血清进行Western blotting试验,结果表明所表达的融合蛋白产物分子质量与预期的65．3 ku相符。  相似文献   

Evaluation of Cluster Bean Meal Cyamposis fefrugonoloba as a Dietary Protein Source for Common Carp Cyprinus carpio L.     

Deyab M.  S. D. El-Saidy Magdy M.  Gaber Ahmed SA.  Abd-Elshafy 《Journal of the World Aquaculture Society》2005,36(3):311-319

Abstract— A12-wk feeding experiment was conducted to study the feasibility of utilizing cluster bean seed meal (CBM) as a replacement for fishmeal in the diets for common carp Cyprnus carpio fingerlings. The replacement levels of cluster bean meal protein in the diets were 25, 50, 75, and 100% of the total fishmeal protein. All diets were isonitrogenous at 33.1% crude protein and isocaloric at 4.8 Kcal/g. There were significant differences in the final individual weights, weight gains, specific growth rates (SGR %/d), feed conversion ratios (FCR), feed efficiency ratio (FER), protein efficiency ratios (PER), and feed intake among fish group ( P × 0.05). Common carp fed the diet containing 50% (CBM) exhibited comparable growth to those fed a fishmeal-based diet. Digestibility of protein, energy and lipid decreased with increasing levels of CBM above 50% of total fishmeal replacement in the diet. Incorporation of CBM in diets significantly affected the dry matter, protein, and energy of whole fish body. These results suggest that CBM can replace 50% of the fishmeal in diet for common carp.  相似文献   

Survival of Mycobacterium avium subsp paratuberculosis in amitraz cattle dip fluid     

GJ EAMENS  SA SPENCE  MJ TURNER 《Australian veterinary journal》2001,79(10):703-706

OBJECTIVE: To determine the survival time of Mycobacterium avium subsp paratuberculosis in amitraz-based cattle dip fluid derived from an active dip site in northern New South Wales. PROCEDURE: Following inoculation of triplicate 5 L containers with faeces (0.5 g/L) from a clinical case of bovine paratuberculosis, samples collected up to 8 weeks after inoculation were examined by conventional and radiometric culture. M a paratuberculosis colonies were enumerated on solid media. RESULTS AND CONCLUSIONS: M a paratuberculosis survived in amitraz cattle dip fluid for up to 2 weeks, but not 3 weeks. Where 1% of solids in dip fluid is derived from a clinical case of paratuberculosis, dip fluid may contain viable M a paratuberculosis for at least 2 weeks. These findings have implications for the management of cattle dip sites.  相似文献   

Effects of Gymnadenia Conopsea Polysaccharide Extract on Growth Performance,Antioxidant Function and Meat Quality of House-fed Mutton Sheep under Oxidative Stress     

SA Ruli  YANG Bin  GE Genhasi  QIAN Linna  ZHAO Shihua 《畜牧兽医学报》1956,51(9):2187-2196

The purpose of this experiment was to study the effects of Gymnadenia conopsea polysaccharide (GCP) extract on growth performance, meat quality and antioxidant function house-fed mutton sheep under oxidative stress, so as to provide experimental basis for extensive research and development of Gymnadenia conopsea and its extracts. Twenty 6-month-old healthy male Small-Tailed Han sheep with body weight of (35.0 ±4.0) kg were randomly divided into two groups. The experimental group (n=10) and control group(n=10). The whole experiment lasted for 42 d and was divided into 3 periods: the first 15 days were the blank period; the middle 15 days were the treatment period; the last 12 days were the observation period ofter modeling. From the first day of the treatment period, the control group was fed with basal diet, and the experimental group was fed pellet basal diet containing 30 mg·kg^-1 GCP extract. On the 15th day of the treatment period, the sheep in each group were intraperitoneally injected with 10 mg·kg^-1 Diquat solution according to sheep body weight(BW), and then fed for another 12 days. On the 1 st, 15th, 30th, 33rd, 36th, 39th and 42nd morning of the whole experimental period, fasting weight and blood samples were collected before feeding. The serum was separated and the antioxidant indexes (SOD, GSH-Px, T-AOC, MDA) in serum were determined. On the 42nd day of the whole experimental period, 5 sheep randomly selected from each group were slaughtered and meat samples were collected to determine the meat quality. The results showed that the addition of GCP extract to the diet of the sheep could increase the average daily feed intake (P<0.05). When the body was subjected to oxidative stress, the indexes of daily gain and antioxidant enzymes recovered rapidly on experimental group, the mutton drip loss decreased significantly, and the mutton sheer force increased significantly(P<0.05). The results indicated that GCP extract could improve the growth performance of mutton sheep, and alleviated the decline of growth performance and antioxidant performance caused by oxidative stress, and had a certain effect of antioxidant stress.  相似文献   

黄土沟壑区旱地仁用杏品种不同花型的数量分布及遗传分析   总被引：2，自引：1，他引：1  

撒文清  魏安智  杨途熙  张睿  杨恒 《西北林学院学报》2004,19(1):50-51

对黄土沟壑区旱地条件下"龙王帽"、"白玉扁"的不同花型数量分布及遗传力进行了分析,结果表明:"龙王帽"的有效花比例高达91.8,具有较大的丰产潜力,宜作为旱地主栽品种;"白玉扁"有效花比例仅为41.2,雌蕊败育率较高,丰产潜力小,仅宜作为授粉品种;花型主要受遗传因素控制(遗传力高达0.99),环境影响较小.  相似文献   

沙芥愈伤组织培养的初步研究   总被引：2，自引：0，他引：2  

张睿  魏安智  杨途熙  撒文清  杨恒 《西北林学院学报》2005,20(3):84-86

采用沙芥种子作为无菌外植体来源时,以0.1升汞消毒10～12min的效果较好.下胚轴和子叶均在MS+2,4-D 0.01 mg·L-1+NAA 2 mg·L-1+6-BA 0.5 mg·L-1+3.0蔗糖+0.5琼脂(pH5.8)中可诱导出愈伤组织.愈伤组织的最佳继代培养基为MS+NAA 2mg·L-1+KT0.5 mg·L-1+3.0蔗糖+0.5琼脂(pH5.8).  相似文献   

甘露寡糖对断奶仔猪肠道菌群的影响     

萨立富  边连全  潘树德 《吉林畜牧兽医》2005,(11):7-8

选取28日龄断奶的三元[杜×(大×长)]杂交仔猪96头,平均体重为(7．46±0．38)kg,按体重和 性别随机分为4个处理组,每个处理组3个重复,每个重复8头仔猪,公母各半。第1个处理组为空白 组,第2、3、4个处理组在相同的基础日粮中分别添加0．1％、0．2％和0．4％的甘露寡糖,研究甘露寡糖对断 奶仔猪肠道菌群的影响。结果表明:1、各实验组肠道大肠杆菌的浓度极显著低于对照组。2、第3组和 第4组其结肠、盲肠和直肠中双歧杆菌增殖显著。第2组中只有结肠中双歧杆菌显著高于对照组,但盲 肠和直肠中双歧杆菌增殖不明显。3、各个处理组肠道中乳酸杆菌的浓度与对照组相比差异不显著。  相似文献   

氨气对肉鸡健康的影响及应对措施   总被引：1，自引：0，他引：1  

孙永波  邢焕  栾素军  王亚  萨仁娜  张宏福 《动物营养学报》2017,29(11)

氨气是鸡舍内危害最大的气体,长期氨气应激不利于肉鸡的健康生长。加强对肉鸡舍氨气浓度的监测,研究氨气对肉鸡健康的影响以及减排措施对指导鸡舍氨气浓度的合理调控和健康养殖的发展具有重要意义。本文主要通过综述舍内氨气的来源,氨气对肉鸡生长性能、屠宰性能和肉品质、免疫功能、呼吸道、繁殖性能、福利等方面的影响以及舍内氨气的监测和减排措施,为深入研究氨气对肉鸡健康影响的作用机制以及合理调控肉鸡舍内氨气浓度提供参考。  相似文献   

盐碱胁迫对燕麦幼苗Na~+、K~+含量及产量的影响     

萨如拉  刘景辉  刘伟  焦伟红  白建慧  王占海 《西北农业学报》2014,23(3):50-54

以燕麦耐盐碱性品种白燕二号和盐碱敏感性品种草莜一号为试验材料,采用中性盐NaCl、Na2SO4及碱性盐Na2CO3、NaHCO3按摩尔比1∶1混合,研究不同盐、碱胁迫对燕麦生物产量及吸收Na+、K+的影响。结果表明,随着盐、碱胁迫浓度的增加,2个品种根、茎、叶中Na+含量增加,K+含量减少,K+/Na+和生物量下降,盐胁迫下K+/Na+及生物量降幅小于碱胁迫,Na+含量增幅小于碱胁迫;且同浓度胁迫下,盐胁迫较碱胁迫叶片中Na+含量较低,K+含量、K+/Na+较高。2个品种间存在差异,盐、碱胁迫下白燕二号中增加的Na+含量小于草莜一号,减少的K+含量、K+/Na+及生物量小于草莜一号,尤其在高盐、碱胁迫下叶片中K+含量及K+/Na+远大于同浓度下的草莜一号;白燕二号比草莜一号根中截留Na+和地上部选择性吸收K+能力强。  相似文献