Genome editing of Nf1, Pten,and Trp53 in neonatal mice induces glioblastomas positive for oligodendrocyte lineage transcription factor 2     

Hiromi Yamamoto  Keisuke Yamamura  Haruka Nagasaki  Takamasa Suzuki  Fumiko Ninomiya  Kenji Matsubara  Naomoto Harada  Shuichi Ohkubo 《Journal of toxicologic pathology》2021,34(4):359

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Formation of the density profile and its effects on the properties of fiberboard   总被引：1，自引：1，他引：0  

Ee Ding Wong  Min Zhang  Guangping Han  Shuichi Kawai  Qian Wang 《Journal of Wood Science》2000,46(3):202-209

Two main types of fiberboards were produced using lauan (Shorea spp.) fibers with an isocyanate resin as the binder; fiberboard with a flat, homogeneous (homoprofile), and typical U-shaped (conventional) density profile along the board thickness. The processing parameters included manipulation of mat moisture content distribution, press closing speed, and hot pressing method. The results are summarized as follows: (1) A larger variation was observed in the peak density (PD) and core density (CD) of fiberboards at 0.5g/cm³ mean density (MD) level than in those at 0.7 g/cm³. Generally, PD showed a greater variation than CD, irrespective of MD level. (2) Boards produced using two-step hot pressing recorded substantially higher PD with reduced CD. (3) Multiple regression analysis showed that CD and PD could be calculated based on the other profile defining factors, and a rough estimation for peak distance and gradient factor was possible. (4) Based on static bending, conventional fiberboard had a higher modulus of rupture (MOR) than the homo-profile board but a similar modulus of elasticity (MOE). (5) At 0.5 g/cm³ the MOR and dynamic MOE of fiberboard increased by up to 67% and 62%, respectively, when the PD increased from 0.5 to 1.07 g/cm³. Similarly, an increase of PD from 0.7 to 1.1 g/cm³ resulted in corresponding increases of 55% and 34% in the MOR and dynamic MOE of 0.7 g/cm³ boards. (6) The internal bond strength and screw withdrawal resistance were almost entirely dependent on the CD and MD, respectively. (7) Homo-profile fiberboards registered higher thickness swelling and water absorption than conventional fiberboards throughout the dry/wet conditioning cycle.  相似文献   

Effects of dietary phosphorus level on non-faecal phosphorus excretion from yellowtail (Seriola quinqueradiata Temminck & Schlegel) fed purified and practical diets     

Pallab Kumer Sarker  Shuichi Satoh  Haruhisa Fukada  & Toshiro Masumoto 《Aquaculture Research》2009,40(2):225-232

Non-faecal phosphorus (P) was determined for large yellowtail to estimate a minimum available P requirement (Experiment  1) and to justify inorganic P supplementation in a fish meal-based diet (Experiment 2). In Experiment 1, purified diets with incremental P concentrations were fed to yellowtail (mean weight 917 g) at a feeding rate of 1.5% of body weight. The peaks of non-faecal P excretion appeared 5–6 h after feeding in fish fed more than 4.5 g available P kg⁻¹ dry diet. Broken-line analysis indicated that the minimum available P requirement was 4.4 g kg⁻¹ dry diet. In Experiment 2, a purified diet (PR) containing 6.5 g available P kg⁻¹ and a fish meal-based diet with (F1) and without (F0) additional phosphorus were fed to yellowtail (mean weight 1.1 kg) at 1.5% (PR) and 2% (F0 and F1) feeding rates respectively. There was no significant difference in P excretion between fish fed the F0 (5.5 g soluble P kg⁻¹ dry diet) and the PR diet. However, significantly higher (34.5%) amounts of non-faecal P excretions (7.4 g soluble P kg⁻¹ dry diet) were found in fish fed F1 compared with the F0 diet. This suggested that there was an excess of dietary P in the F1 diet and that supplementation is not needed in fish meal-based diets for large yellowtail.  相似文献   

Effect of nucleotides supplementation to low‐fish meal feed on long‐chain polyunsaturated fatty acid composition of juvenile rainbow trout Oncorhynchus mykiss     

Asep Ridwanudin  Yutaka Haga  Takayuki Katagiri  Shuichi Satoh 《Aquaculture Research》2019,50(8):2218-2230

A feeding trial was conducted to evaluate the effect of nucleotides supplementation to low‐fish meal feed on growth and fatty acid composition of rainbow trout. Six isonitrogenous (42% crude protein) and isolipidic (18% crude lipid) diets were formulated containing fish meal and plant ingredients as main protein sources. The control diet was a basal diet without supplementation of nucleotides, and five experimental diets were prepared by supplementing one of the five different nucleotides in the form of 5′‐monophosphate (0.15%), that is inosine (IMP), adenosine (AMP), guanosine (GMP), uridine (UMP) and cytidine (CMP) onto basal diet. Two hundred forty juvenile rainbow trout with an initial average body weight 9.8 g were randomly distributed into twelve aquaria. After 15 weeks of feeding period, growth performance and feed utilization of rainbow trout were not significantly different among dietary treatments. Dietary GMP, UMP and CMP tended to accumulate crude lipid in the muscle and whole fish body. Moreover, dietary GMP, UMP and CMP significantly increased hepatic 18:3n‐3 and long‐chain homologue 18:4n‐3 and 20:4n‐3 contents. Hepatic 18:2n‐6 content showed also increase in fish fed GMP, UMP and CMP diets, but decreased in long‐chain homologue 20:3n‐6 and 20:4n‐6 contents. Decrease in 20:4n‐6, 20:5n‐3 and 22:6n‐3 contents was also found in the muscle of fish fed IMP, GMP and CMP diets. The present study clearly showed that there was no positive effect of dietary nucleotides on growth of fish, but dietary nucleotides particularly GMP, UMP and CMP altered polyunsaturated fatty acid composition of rainbow trout.  相似文献   

Involvement of granulin in estrogen-induced neurogenesis in the adult rat hippocampus     

Chiba S  Suzuki M  Yamanouchi K  Nishihara M 《The Journal of reproduction and development》2007,53(2):297-307

Recent studies have demonstrated the presence of neurogenesis in the adult mammalian hippocampus, and it has been suggested that estrogen and various growth factors influence the processes of adult neurogenesis. The present study assessed cell proliferation in the dentate gyrus and the mRNA expression levels of granulin, insulin-like growth factor-I (IGF-I), and brain-derived neurotrophic factor (BDNF) in the hippocampus 4 h after treatment with estradiol benzoate (EB) in 3- and 12-month old ovariectomized rats. At 3 months of age, mRNA expression of granulin precursor and cell proliferation were increased by EB treatment, although the mRNA expressions of IGF-I and BDNF remained unchanged. At 12 months of age, however, neither mRNA expression of the three genes nor cell proliferation in the dentate gyrus were affected by EB treatment. In addition, 17beta-estradiol enhanced the proliferation of neural progenitor cells derived from hippocampal tissue of 3-month-old female rats in vitro; this was inhibited by neutralization of granulin with specific antibody. These results suggest that estrogen induces granulin gene expression in the hippocampus and that the product of this gene is involved in the mitogenic effects of estrogen in the dentate gyrus, although the responses to estrogen decline with age.  相似文献   

Chemical structural elucidation of total lignins in woods I: fractionation of the lignin in residual wood meal after extraction of milled wood lignin     

Shuichi Hirosawa  Rui Katahira  Fumiaki Nakatsubo 《Journal of Wood Science》2002,48(1):46-50

Residual wood meal after extraction of milled wood lignin (WMEM) ofEucalyptus globulus was extracted with alkali and LiCl/N,N-Dimethylacetamide (DMAc). These agents dissolve mainly hemicellulose and cellulose, respectively. The extractability of WMEM in alkali solutions was influenced by the degree of swelling of the cellulose. Under good swelling conditions, considerable amounts of cellulose and lignin were extracted with the hemicellulose. Maximum extractability was about 60% of the WMEM under optimum conditions (3 M or 5 M LiOH or 3M NaOH solution). Some portion of cellulose was extracted with LiCl/DMAc at room temperature. Thus, lignin inE. globulus WMEM was divided into three fractions: hemicellulose-lignin fraction, cellulose-lignin fraction, and insoluble-lignin fraction.Part of this work was presented at the 49th annual meeting of the Japan Wood Research Society, Tokyo, April 1999; and at the 50th annual meeting of the Japan Wood Research Society, Kyoto, April 2000  相似文献   

Detection of intra- and interspecific variation of the dry rot fungus <Emphasis Type="Italic">Serpula lacrymans</Emphasis> by PCR-RFLP and RAPD analysis     

Sakae?HorisawaEmail author  Yoh?Sakuma  Katsuhiko?Takata  Shuichi?Doi 《Journal of Wood Science》2004,50(5):427-432

We investigated a genotype-based assay to discriminate the dry rot fungi Serpula lacrymans. DNAs were extracted from 74 isolates from the northern half of Japan, and internal transcribed spacers (ITS) were amplified by polymerase chain reaction. Genotypes of isolates were checked by restriction fragment length polymorphism (RFLP) using two enzymes, Taq I and Hha I. Among the 74 isolates identified as S. lacrymans in terms of morphologic features, 5 isolates were shown to have been misidentified. Random amplified polymorphic DNA (RAPD) analysis was conducted in order to detect the intraspecific diversity of S. lacrymans isolated in Japan. Because no relation between geographical origin and genetic distances was observed, the intraspecific diversity of S. lacrymans is suggested to be small.Part of this report was presented at the 52nd Annual Meeting of the Japan Wood Research Society, Gifu, April 2002  相似文献   

Changes in surface properties of tropical wood species exposed to the Indonesian climate in relation to mold colonies     

Yanni Sudiyani  Sakae Horisawa  Keli Chen  Shuichi Doi  Yuji Imamura 《Journal of Wood Science》2002,48(6):542-547

Changes in mold populations and genera on the exposed surfaces of tropical hardwoods — albizia (Paraserianthes falcata), kapur (Dryobalanop lanceolata), mahoni (Switenia macrophylla), nangka (Artocarpus heterophyllus), puspa (Schima wallchii) — were investigated. The wood specimens were exposed to the Indonesian climate for 32 weeks. Properties including mass loss, wettability, mold growth (colony-forming units), and mold genera were evaluated. The change in properties after exposure was significantly affected by the wood species, but there was no clear relation between mass loss and the initial chemical components or between wettability and wood density. The number of mold populations was different by exposure period and wood species, but there was no significant effect of climate conditions, such as rainfall and ultraviolet radiation. Of the genera identified,Aureobasidium, Cladosporium, andPenicillium were dominant molds on the exposed wood surfaces.  相似文献   

Raman spectroscopic study on the behavior of boric acid in wood     

Shigeru Yamauchi  Shuichi Doi 《Journal of Wood Science》2003,49(3):227-234

 Raman spectra of Japanese cedar (Cryptomeria japonica D. Don) treated by vacuum impregnation with aqueous boric acid solutions (8.1 × 10⁻² to 7.29 × 10⁻¹ mol dm⁻³) were recorded using a near-infrared laser as an excitation source. Raman spectroscopic measurements were carried out on treated wood blocks of two sizes: 20(T) × 20(R) × 5(L) mm (A-type) and 15(T) × 15(R) × 50(L) mm (B-type). Our attention was focused on a prominent band (ν ₁) assigned to a symmetrical stretching vibration of the BO₃ group because no Raman band due to boron species was observed except bands of B(OH)₃. We observed a change in ν ₁ band intensity with increasing boric acid concentration in the aqueous solution used to treat the A-type wood blocks and investigated the correlation between the intensity and the peak-top wavenumber. Raman line maps in the longitudinal direction of the treated B-type wood blocks revealed that B(OH)₃ is concentrated near the cut ends. These results suggested that two groups of B(OH)₃ exist in wood in terms of the chemical species in the nearest neighbor sphere. Received: March 11, 2002 / Accepted: June 26, 2002  相似文献   

Effect of carbon dioxide-air concentration in the rapid curing process on the properties of cement-bonded particleboard     

Dede Hermawan  Toshimitsu Hata  Shuichi Kawai  Wakatsu Nagadomi  Yasuo Kuroki 《Journal of Wood Science》2002,48(3):179-184

This study deals with the effects of carbon dioxide (CO₂)-air concentration in the rapid curing method on the properties of cement-bonded particleboard manufactured using conventional cold pressing as the setting method. The hydration of cement was examined using X-ray diffractometry, thermal gravimetry, and scanning electron microscopy. The results are as follows: (1) The properties of CO₂-cured boards improved with increasing CO₂ concentration. When 10% or 20% CO₂ was applied for 10 min of curing time, the properties of the CO₂-cured boards were comparable to those obtained by conventional 2-week curing. (2) The hydration process of cement could be accelerated within several minutes using CO₂ curing, even with a low concentration of 10%–20% CO₂; a reduction in calcium hydroxide was observed followed by rapid formation of calcium carbonate.  相似文献