Isolation and identification of indigestible pyroglutamyl peptides in an enzymatic hydrolysate of wheat gluten prepared on an industrial scale     

Higaki-Sato N  Sato K  Esumi Y  Okumura T  Yoshikawa H  Tanaka-Kuwajima C  Kurata A  Kotaru M  Kawabata M  Nakamura Y  Ohtsuki K 《Journal of agricultural and food chemistry》2003,51(1):8-13

An enzymatic hydrolysate of wheat gluten was further digested in vitro with porcine pepsin and pancreatin to obtain an indigestible peptide. Indigestible pyroglutamyl peptide was isolated from the digest by strong cation-exchange, size-exclusion, and reversed-phase chromatographies. The pyroglutamyl peptide was digested with pyroglutamate aminopeptidase, and the digest was reacted with phenyl isothiocyanate. The resultant phenylthiocarbamyl (PTC) peptides were purified by reversed-phase HPLC by using binary gradient elution with ammonium acetate buffer, pH 6.0, and acetonitrile. The PTC peptides were analyzed with an automatic peptide sequencer on the basis of the Edman degradation method with a modified program. Some pyroglutamyl peptides were also analyzed by fast-atom bombardment ionization mass spectrometry without the pyroglutamate amino peptidase digestion. Consequently, pyroGlu-Asn-Pro-Gln, pyroGlu-Gln-Gln-Pro-Gln, pyroGlu-Gln-Pro-Gln, pyroGlu-Gln-Pro-Gly-Gln-Gly-Gln, pyroGlu-Gln, pyroGlu-Gln-Pro, pyroGlu-Ile-Pro-Gln, pyroGlu-Ile-Pro, pyroGlu-Gln-Pro-Leu, pyroGlu-Gln-Phe-Pro-Gln, pyroGlu-Ser-Phe-Pro-Gln, pyroGlu-Phe-Pro-Gln, and pyroGlu-Gln-Pro-Pro-Phe-Ser were identified.  相似文献   

Angiotensin I-converting enzyme inhibitory peptides in a hydrolyzed chicken breast muscle extract   总被引：4，自引：0，他引：4  

Saiga A  Okumura T  Makihara T  Katsuta S  Shimizu T  Yamada R  Nishimura T 《Journal of agricultural and food chemistry》2003,51(6):1741-1745

The blood pressure of spontaneously hypertensive rats (SHRs) decreased after oral administration of an extract prepared from chicken breast muscle, falling maximally to 50 mmHg lower than before. This effect continued for at least 4 h after administration. The peptides possessing hypotensive activity in the chicken extract were examined by measuring the inhibitory activity (IC(50)) against angiotensin I-converting enzyme (ACE). The inhibitory activity of the chicken extract was 1060 mg%, whereas the activity of the extract treated with an Aspergillus protease and gastric proteases (trypsin, chymotrypsin, and intestinal juice) became stronger, reaching 1.1 mg%. Peptides in this hydrolysate of the extract were isolated by HPLC on a reversed-phase column, and their N-terminal sequences were analyzed. Three peptides possessed a common sequence, Gly-X-X-Gly-X-X-Gly-X-X, which was homologous with that of collagen. The peptide Gly-Phe-Hyp-Gly-Thr-Hyp-Gly-Leu-Hyp-Gly-Phe showed the strongest inhibitory activity (IC(50) = 42 microM).  相似文献   

Surgical management and long-term outcome of dogs with cervical spondylomyelopathy with an anchored intervertebral titanium device     

JC King  GS Corfield  JG Mouatt  CY Kan  PA Moses 《Australian veterinary journal》2020,98(4):156-163

  相似文献   

Difference in mass concentration of airborne dust during circular sawing of five wood-based materials     

Kiyohiko Fujimoto  Tsutomu Takano  Shogo Okumura 《Journal of Wood Science》2011,57(2):149-154

The object of this study was to compare the mass concentration of airborne dust during circular sawing of five wood-based materials: solid sugi (Cryptomeria japonica) lumber, tropical hardwood plywood, softwood plywood, particleboard, and medium-density fiberboard. Specimens were sawn at a constant feed per tooth (0.05 mm) using two saw speeds. The mass concentration of airborne dust of diameter 7.07 μm or less (respirable dust) was measured with a light-scattering dust monitor. The mass concentration showed a log-normal distribution, and the geometric means of mass concentration at saw speeds of 2000 and 3000 rpm were 2.33 and 2.89 mg/m³ for tropical hardwood plywood, 1.13 and 2.84 mg/m³ for particleboard, 0.91 and 2.28 mg/m³ for medium-density fiberboard, 1.09 and 1.38 mg/m³ for softwood plywood, and 0.32 and 0.66 mg/m³ for sugi lumber. The mass concentration for all five wood-based materials increased with the revolution speed of the circular saw.  相似文献   

Effect of grain direction on transmittance of 100-GHz millimeter wave for hinoki (<Emphasis Type="Italic">Chamaecyparis obtusa</Emphasis>)     

Soichi?TanakaEmail author  Yuko?Fujiwara  Yoshihisa?Fujii  Shogo?Okumura  Hiroyoshi?Togo  Naoya?Kukutsu  Tadao?Nagatsuma 《Journal of Wood Science》2011,57(3):189-194

The attenuation coefficients of 100-GHz millimeter waves polarized linearly were measured for cross-cut, quarter-sawn, and flat-sawn boards of hinoki (Chamaecyparis obtusa) that were 0.2–2.0 cm thick. This was done to examine the applicability of free-wave propagation theory for applying electromagnetic waves to wood. It was found that the transmittance of a millimeter wave through the specimen boards was lower when the fiber direction of a board was parallel to the direction of the electric field of the incident wave than when the fiber direction was perpendicular to the electric field, and there was little difference in the transmittance between the tangential and radial directions for the former case. These findings can be quantitatively explained by using propagation theory and the dielectric properties of wood.  相似文献   

No Involvement of Lysophosphatidic Acid Receptor-3 in Cell Migration of Mouse Lung Tumor Cells Stimulatedby 12-O-Tetradecanoylphorbol-13-acetate     

Okumura M  Kato K  Fukui R  Fukushima N  Tsujiuchi T 《Journal of toxicologic pathology》2011,24(3):183-186

  相似文献   

Isolation, characterization, and quantitative analysis of ceruloplasmin from horses.   总被引：3，自引：0，他引：3  

M Okumura  T Fujinaga  K Yamashita  N Tsunoda  S Mizuno 《American journal of veterinary research》1991,52(12):1979-1985

Ceruloplasmin (Cp) was isolated from fresh equine plasma by precipitation, cellulose chromatography, and improved ion-exchange chromatography. Purified equine Cp is a glycoprotein having a molecular weight of approximately 115,000. In electrophoresis, equine Cp migrated to the alpha 1-globulin region, its isoelectric point was about 4.15 and consisted of about 890 amino acid residues. Serum Cp concentration was measured by use of the single radial immunodiffusion method. In clinically normal horses, the mean (+/- SD) serum Cp concentration of newborn foals was 2.87 +/- 0.40 mg/ml and that of 3-month-old foals was 5.02 +/- 0.92 mg/ml, which was similar to the adult value. It reached a peak of 6.06 +/- 0.74 mg/ml in 2-year-old horses. The Cp concentration in mares was not statistically different for the perinatal period, but it decreased immediately before and after delivery. Concentration of Cp increased at 6 days after IM administration of turpentine oil, castration, or jejunojejunostomy in adult horses, and increased to peak values twice as high as baseline values at 7 to 14 days, returning to baseline values at 28 days after treatment. We concluded that equine serum Cp is an acute-phase reactive protein increased in the intermediary or later phase of acute inflammation.  相似文献   

Intramuscular fat deposition in principal muscles from twenty-four to thirty months of age using identical twins of Japanese Black steers     

Okumura T  Saito K  Sakuma H  Nade T  Nakayama S  Fujita K  Kawamura T 《Journal of animal science》2007,85(8):1902-1907

The changes in i.m. fat deposition in the principal muscles [M. semitendinosus, M. semimembranosus, M. psoas major, M. latissimus dorsi, LM (7th to 8th and 10th to 11th thoracic vertebrae), and M. supraspinatus] from 24 to 30 mo of age were investigated using identical twins of Japanese Black steers. Four sets of identical twins of Japanese Black steers were used in this study. Animals were fattened from 10 to 24 or 30 mo of age for each pair of identical twins. Body weights of twin steers slaughtered at 24 and at 30 mo of age were similar at 10 mo of age and thereafter up to 24 mo of age. The changes in serum concentration of vitamin A, glucose, total cholesterol, albumin, and total protein were similar in each pair of twins during the first fattening stage (10 to 24 mo). Fat contents of LM (7th to 8th thoracic vertebrae) at 24 and 30 mo of age were 37.0 and 42.4%, respectively (P < 0.05). Moreover, in the principal muscles, except M. semimembranosus and M. supraspinatus, fat content at 30 mo of age was greater than at 24 mo of age (P < 0.05). The proportional increase in fat content from 24 to 30 mo of age was greatest in M. semitendinosus (+58.7%) and least in M. supraspinatus (+6.1%). These results demonstrate that i.m. fat continues to increase after 24 mo of age, and the rates of i.m. fat deposition and the ages when i.m. fat is deposited are different for every muscle.  相似文献   

Herschel detects a massive dust reservoir in supernova 1987A     

Matsuura M  Dwek E  Meixner M  Otsuka M  Babler B  Barlow MJ  Roman-Duval J  Engelbracht C  Sandstrom K  Lakićević M  van Loon JT  Sonneborn G  Clayton GC  Long KS  Lundqvist P  Nozawa T  Gordon KD  Hony S  Panuzzo P  Okumura K  Misselt KA  Montiel E  Sauvage M 《Science (New York, N.Y.)》2011,333(6047):1258-1261

We report far-infrared and submillimeter observations of supernova 1987A, the star whose explosion was observed on 23 February 1987 in the Large Magellanic Cloud, a galaxy located 160,000 light years away. The observations reveal the presence of a population of cold dust grains radiating with a temperature of about 17 to 23 kelvin at a rate of about 220 times the luminosity of the Sun. The intensity and spectral energy distribution of the emission suggest a dust mass of about 0.4 to 0.7 times the mass of the Sun. The radiation must originate from the supernova ejecta and requires the efficient precipitation of all refractory material into dust. Our observations imply that supernovae can produce the large dust masses detected in young galaxies at very high redshifts.  相似文献   

Substrate specificity of the nateglinide/H(+) cotransport system for phenolic acids   总被引：2，自引：0，他引：2  

Saito Y  Itagaki S  Otsuka Y  Kobayashi Y  Okumura H  Kobayashi M  Hirano T  Iseki K 《Journal of agricultural and food chemistry》2005,53(15):6100-6104

In clinical, patients usually take many kinds of drugs at the same time. Thus, drug-drug interactions involving transporters can often directly affect the therapeutic safety and efficacy of many important drugs. However, there have been few studies on food-drug interactions involving transporters. Dietary polyphenols have been widely assumed to be beneficial for human health. Polyphenols are found ubiquitously, and they are commercially prepared and used as functional foods. We have reported that ferulic acid, which is one of the most well-known polyphenols and is used as a functional food, affected the transport of nateglinide, an antidiabetic drug, by Caco-2 cells. In this study, we investigated the effects of other polyphenols on the nateglinide/H(+) transport system. We report here that caffeic acid and p-coumaric acid have a different inhibitory manner on the uptake of nateglinide. The results of this study are useful to identify the substrate specificity of the nateglinide/H(+) cotransporter.  相似文献