转甜菜碱醛脱氢酶基因增强羽衣甘蓝的耐盐性研究     

李春枝  潘彩霞  刘宏伟  刘冰  罗晓明  李大红 《北方园艺》2018,(5):33-38

羽衣甘蓝(Brassica oleracea L.var.accephala DC.)是目前常用观赏植物。该研究中,菠菜BADH(甜菜碱醛脱氢酶)基因用农杆菌介导转入了羽衣甘蓝中,共获得了7株转基因植株,并经PCR、Southern blot和实时荧光定量PCR分析。结果表明:BADH基因已整合到羽衣甘蓝基因组并在转基因植物中表达。在盐胁迫下,转基因植物中的BADH酶活性明显高于野生型植物,而转基因植株的MDA含量及相对电导率明显低于野生型植物。表明BADH活性增加,膜的渗透性保护能力也增强,转基因植物的抗逆性增加。同时,在盐胁迫下转基因羽衣甘蓝植株平均主根长、鲜质量、成活率和叶绿素含量均显著高于野生植株,而干质量/鲜质量明显低于野生植株。转基因羽衣甘蓝耐盐性明显增强,该转基因植物可以为羽衣甘蓝抗性育种提供分子基础。  相似文献   

银杏古树不同产地和家系子代生长及叶片药用成分含量的差异   总被引：1，自引：0，他引：1  

于国栋  夏米蕊  杨新成  乔鸿翔  潘云  付近 《北方园艺》2018,(11):144-150

以4个产地24株银杏古树家系的实生苗为试材,采用单因素完全随机设计于云南同一立地条件下培育,研究不同产地及家系间子代的生长指标及叶片药用成分的差异。结果表明:苗龄0.6年时,不同产地间,平均叶面积、叶片数、叶片厚、地径和苗高分别为17.52~20.17cm2、5.6~8.4片·株-1、0.21~0.24mm,0.42~0.52cm和9.69~13.17cm,除叶片厚无显著差异(P0.05)外,其它指标间均具有显著或极显著差异(P0.05或P0.01);家系间,以上5个指标分别为11.12~27.41cm2、4.3~12.5片·株-1、0.15~0.29mm、0.29~0.56cm和6.18~13.65cm,家系间5个指标均呈极显著差异(P0.01)。同龄苗木的家系折干黄酮和内酯含量分别为1.49%~1.91%和0.48%~0.84%,24株家系中,黄酮和内酯含量相对较高的优良家系分别有8株和4株,二者含量较高的优良家系仅1株。银杏叶片内黄酮及内酯含量与生长指标间呈现未达显著水平的负相关。药用为主的银杏叶用人工原料林培育的良种选育应采用产地-家系-单株的联合选育模式。  相似文献   

桂林市水果型节瓜新品种秋季引种比较试验     

熊俏  覃柳兰  谭海文  杨建明  潘玲华  滕献友 《长江蔬菜》2018,(8):56-58

对引进的6个水果型节瓜品种进行了比较,以筛选出适合桂林地区秋季种植的水果型节瓜新品种,促进节瓜产业快速发展。试验结果表明,甜仙子1号、甜仙子2号、翠玉、玉如意4个新品种综合性状好、口感好、产量高,适合在桂林地区秋季栽培推广生产。  相似文献   

莲藕加工废料综合利用研究进展     

惠林冲  唐猛  杨海峰  潘美红  陈薇  何林玉  薛萍  缪美华  陈振泰 《长江蔬菜》2018,(4):31-34

综述了莲藕加工废料的主要营养成分和综合利用研究进展,介绍莲籽壳和莲藕渣的主要成分是纤维素、多糖、淀粉等;对其有效成分的提取及作为食用菌、基质等产品加工的原辅料,提出废料综合利用存在的问题及对策。  相似文献   

包衣技术提高药物药学性能的研究进展     

李超  罗万和  周凯翔  陈冬梅  潘源虎  陶燕飞  谢书宇 《中国畜牧兽医》2018,45(11):3271-3278

兽药对畜牧生产中的疾病防控和健康养殖意义重大,但由于某些药物味苦、对胃黏膜刺激性大、稳定性差和药理作用部位靶向性差等限制了其在临床上的使用。包衣技术在改善药物适口性、提高药物稳定性和实现药物控缓释和靶向性等方面具有显著优势。作者总结了国内外关于包衣技术在改善药物适口性、提高药物稳定性、制备控缓释剂型和高效靶向释药剂型方面的研究进展,分析了包衣膜的形成原理,剖析了几种常见包衣技术的优缺点和其产业化面临的挑战,在此基础上展望了包衣技术在兽药领域的应用前景,以期为基于包衣技术的兽药新制剂的开发提供新思路。  相似文献   

论先秦杂家的存在问题     

潘俊杰 《湖南农业大学学报(自然科学版)》2008,(1)

虽然在学术界有许多学者认为,先秦时期杂家"学无所主"、"调和"、"折衷"百家之学,杂家不成其为一个学派,甚至将其归属于黄老道家。但笔者通过多年的研究,发现先秦杂家有自己独特的学术宗旨、理论方法和构筑思想体系的思维模式,先秦杂家有自己的代表人物和作品,而且,先秦杂家和汉初诸子有着学术上的传承关系。  相似文献   

互花米草入侵对滨海湿地重金属的富集迁移效应     

庞晓艳  潘继花  刘飞  陈长东  袁显龙  叶敦雨  陈迎港 《安徽农业大学学报》2022,49(6):1006

为探究互花米草（Spartina alterniflora）入侵对滨海湿地沉积物重金属的富集迁移效应,为滨海湿地生态保护与合理利用提供依据,在山东省日照市黄家塘湾滨海湿地选取不同互花米草入侵年限的斑块,以5 cm为间隔采集0～30 cm沉积物样品和互花米草样品,分析沉积物和互花米草不同部位中重金属元素Cu、Zn、Pb和Cd的含量,并采用地累积指数法、潜在生态风险评价法、生物富集系数及转运系数等方法进行评价。结果表明：湿地沉积物重金属Cu、Zn、Pb和Cd 的含量范围为5.94～31.94 mg(kg^-1,18.59～56.87 mg(kg^-1,4.76～30.36 mg(kg^-1,0.021～0.083 mg(kg^-1,整体低于研究区域重金属环境背景值;但Cu、Pb在0～15 cm内存在轻度污染;与光滩湿地相比,互花米草入侵湿地沉积物中重金属含量增加,且随互花米草入侵年限增加,沉积物重金属含量呈增加趋势,在上层（0～15 cm）表现尤为显著;互花米草不同部位对重金属的累积存在一定差异,大致表现根>叶>茎;不同重金属在根部的富集效应表现为Cd > Zn > Cu > Pb,互花米草对重金属转运效应为Pb > Zn > Cu > Cd。相关分析表明,沉积物中重金属的累积与互花米草入侵对湿地沉积物粒度组成、有机质含量及pH的改变密切相关。不同重金属在沉积物-植物体系的迁移转运差异则主要与互花米草各部位对重金属元素的吸收差异有关。  相似文献   

Role of sphingosine 1-phosphate on high glucose-induced vascular endothelial cell dysfunction     

LIU Wei-hua  LIN Shuang-feng  SHI Ji-xiang  PAN Ting  CHEN Qiu-mei  WANG Shuo-ting  SHANG Hui 《园艺学报》2016,32(2):245-250

AIM: To explore the role of sphingosine 1-phosphate (S1P) in the dysfunction of vascular endothelial cells exposed to high glucose. METHODS: In human aortic endothelial cells cultured under high-glucose (22 mmol/L glucose) medium, nitric oxide (NO) level, polymorphonuclear neutrophil-endothelial cell adhesion rate, protein level of intercellular adhesion molecule-1 (ICAM-1), migration of endothelial cells and Akt/endothelial nitric oxide synthase (eNOS) pathway activation were observed after S1P, sphingosine kinase-1 inhibitor and/or Akt inhibitor treatments. RESULTS: S1P decreased NO level, increased polymorphonuclear neutrophil adhesive rate, enhanced ICAM-1 protein level, and inhibited migration of endothelial cells and activation of Akt/eNOS pathway in endothelial cells cultured under high-glucose condition. Sphingosine kinase-1 inhibitor, which reduced S1P content, significantly improved the above endothelial cell function indexes and restored the activation of Akt/eNOS pathway. CONCLUSION: S1P promoted high glucose-induced dysfunction of endothelial cells probably by inhibiting the activation of Akt/eNOS signal pathway. Targeting S1P is expected to become one of potential treatment strategies to reduce endothelial cell dysfunction.  相似文献   

Fluoxetine regulates hippocampal synaptic plasticity in CUMS depression rats     

SHEN Zhong-fei  WANG Zhi-jian  PAN Wei-wei  GUO Yan-jun  FU Chun-yan  YUAN Feng-feng 《园艺学报》2016,32(9):1642-1647

AIM: To investigate the role of fluoxetine in the hippocampal synaptic plasticity in chronic unpredictable mild stress (CUMS) depression rats and its effect on mTOR and autophagy signaling pathways. METHODS: Male Sprague-Dawley rats (n=60) were randomly divided into normal control group, CUMS group and fluoxetine group. The CUMS rat model was established through CUMS combined with solitary raising, and fluoxetine (20 mg·kg^-1·d^-1) was administered via intragastric gavage. The changes of body weight, the ratio of sugar intake and the results of the behavioral test were recorded to identify the modeling. Moreover, the expression of synaptic plasticity-related proteins glial fibrillary acidic protein (GFAP) and synaptophysin (SYP), apoptosis-related proteins Bcl-2 and caspase-3, mTOR signaling proteins mTOR and 4EBP1, and autophagy-related proteins beclin 1 and LC3 were examined by RT-PCR and Western blot.RESULTS: Compared with control group, the body weight, sucrose intake, and total distance and intermediate residence time in the open field test were significantly decreased in CUMS group. The results of RT-PCR and Western blotting showed that the mRNA and protein levels of SYP and GFAP in CUMS group were significantly down-regulated compared with control group. The expression of Bcl-2 in CUMS group was downregulated, while the protein level of cleaved caspase-3 increased. Decreased phosphorylation levels of mTOR and its downstream target molecule 4EBP1 were observed in CUMS group. Besides, the autophagy-related proteins beclin 1 and LC3 were significantly upregulated at mRNA and protein levels. All these results(upregulation or downregulation) were attenuated by the treatment with fluoxetine, and the difference was statistically significant. CONCLUSION: Fluoxetine might improve hippocampal synaptic plasticity and alleviate symptoms of depression by supressing apoptosis/autophagy signaling pathways and upregulating mTOR signaling pathway.  相似文献   

高效液相色谱法测定盐酸林可霉素注射液的含量   总被引：1，自引：0，他引：1  

郑宏伟  潘珏羽  梁靖涵  刘海莲 《中兽医医药杂志》2016,(4):40-41

建立盐酸林可霉素注射液含量测定的高效液相色谱法。色谱柱:C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相:0.05 mol/L硼砂溶液(用85%磷酸溶液调节pH值至5.0)∶甲醇∶乙腈=67∶33∶2(V/V),流速:1 mL/min,检测波长:214 nm,进样量:10μL,柱温:30℃。盐酸林可霉素浓度在0.1-2 mg/m L范围内峰面积与浓度的线性关系良好(R~2=0.999 9)。此法简便、快捷、灵敏度高、重现性好。  相似文献