Analysis of DNA relationships among Aeromonas species by RAPD (randomly amplified polymorphic DNA) typing     

V. Inglis  D. Colquhoun  M. D. Pearson  M. Miyata  T. Aoki 《Aquaculture International》1996,4(1):43-53

Genetic differences between a collection of aeromonads were studied in two laboratories by analysis of randomly amplified polymorphic DNA (RAPD). A single randomly designed primer, generated reproducible profiles of genomic DNA in both laboratories for Aeromonas salmonicida subspecies salmonicida, although the profiles differed between laboratories. Analysis of atypical strains of A. salmonicida and isolates of the A. hydrophila group produced scattered profiles in both laboratories. The uniform fingerprints produced for A. salmonicida subspecies salmonicida indicate genomic homogeneity. The scattered RAPD profiles of the motile aeromonads demonstrate the genomic diversity of this group. A group of unspeciated motile aeromonads gave uniform fingerprints, suggesting the possibility of a genomically homogeneous species. Although the RAPD technique is susceptible to the effects of minor technical variations, this study has demonstrated that where there is DNA similarity, it can be recognized, and where there is diversity, differentiation can be made. RAPD promises to be useful in epidemiological studies for rapid identification of bacteria where a source of reference DNA is available and may be useful in preliminary investigations of relatedness within groups.  相似文献   

The colonization of the alimentary tract and visceral organs of chicks with salmonellas following challenge via the feed: bacteriological findings   总被引：2，自引：0，他引：2  

Y M Xu  G R Pearson  M Hinton 《The British veterinary journal》1988,144(4):403-410

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BIOMETRY AND BIOMETRIKA     

Pearson K 《Science (New York, N.Y.)》1903,17(432):592-594

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Responsible referral for colic surgery     

H Pearson 《Equine veterinary journal》1986,18(4):246-248

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Naturally acquired Pasteurella multocida infection in rabbits: clinicopathological aspects.   总被引：1，自引：0，他引：1       下载免费PDF全文

R F DiGiacomo  Y M Xu  V Allen  M H Hinton    G R Pearson 《Canadian journal of veterinary research》1991,55(3):234-238

A cohort of 41 New Zealand White rabbits, 35 to 60 days old, from twelve litters were followed for twelve weeks for development of pasteurellosis. Eleven of 19 rabbits in five litters acquired Pasteurella multocida infection. The incubation period was difficult to determine as P. multocida infection was detected both before and after the onset of rhinitis. The response of rabbits to infection varied from subclinical infection to death from systemic pasteurellosis. Atrophy of the maxilloturbinates of the nares was detected in rabbits with chronic rhinitis associated with P. multocida infection.  相似文献   

Liver failure attributable to pyrrolizidine alkaloid toxicosis and associated with inspiratory dyspnea in ponies: three cases (1982-1988)     

E G Pearson 《Journal of the American Veterinary Medical Association》1991,198(9):1651-1654

Of 41 equids referred to a veterinary teaching hospital in the Pacific northwest because of dyspnea and inspiratory noise, 3 ponies were diagnosed as having liver failure, most likely attributable to pyrrolizidine alkaloid toxicosis. Dyspnea appeared to be caused by laryngeal and/or pharyngeal paralysis. It is proposed that this paralysis was a manifestation of hepatic encephalopathy. Although these clinical signs are not common for pyrrolizidine toxicosis, practitioners should be aware of the possibility so that misdiagnosis of other causes of inspiratory dyspnea may not be made.  相似文献   

Trypanosoma evansi infection in worked and unworked buffaloes (Bubalus bubalis) in Indonesia     

R. C. Payne  D. Djauhari  S. Partoutomo  T. W. Jones  R. Anne Pearson 《Veterinary parasitology》1991,40(3-4):197-206

The effect of controlled amounts of exercise on the outcome of Trypanosoma evansi infection was studied in groups of swamp buffaloes (Bubalus bubalis) experimentally infected with T. evansi. Daily body temperature, packed cell volume (PCV) and parasitaemia measurements were obtained from each animal for up to 110 days after infection. Exercise did not appear to exacerbate the effect of T. evansi infection in that similar temperature, PCV and parasitaemia profiles were obtained with both exercised and rested animals. Trypanosoma evansi infection, however, had a marked effect on temperature and PCV profiles, both of which could adversely affect an infected animal's work output and work tolerance.  相似文献   

Biological characterisation of Australian isolates of chicken anaemia agent   总被引：1，自引：0，他引：1  

TJ CONNOR  F. McNEILLY  GA FIRTH†  MS McNULTY† 《Australian veterinary journal》1991,68(6):199-201

Three Australian isolates of chicken anaemia agent (CAA) resisted treatment at 70 degrees C for 5 min and chloroform treatment. Although minor antigenic differences were detected using monoclonal antibodies to CAA, the Australian isolates were indistinguishable from the reference Cux-1 and Gifu-1 isolates in cross-immunofluorescence and cross-neutralisation tests employing polyclonal chicken antiserums. The Australian viruses were pathogenic for intramuscularly inoculated 1-day-old SPF chicks, but were less pathogenic for 7-day-old chicks. Thus the Australian isolates of CAA did not differ significantly in these properties from previously characterised CAA isolates from other continents.  相似文献   

A review: relationships of some hormones to growth and carcass quality of ruminants     

H D Hafs  R W Purchas  A M Pearson 《Journal of animal science》1971,33(1):64-71

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Mastitis control: the use of electronic cell counting methods     

J K Pearson  D O Greer  B K Spence 《The Veterinary record》1972,90(17):485-486

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