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71.
不同种植密度和施肥水平对大豆籽粒品质的影响 总被引：12，自引：0，他引：12
本试验自2002年开始至2004年止，从种植密度和施肥水平两方面对大豆脂肪、蛋白质含量的影响进行了分析。结果表明：种植密度对大豆籽粒的脂肪、蛋白质含量具有显著的影响，相关性分析结果显示，种植密度与大豆籽粒的脂肪含量存在着负相关性，与籽粒中的蛋白质含量之间为正相关。中国品种的脂肪含量受种植密度的影响大于美国品种，而中国品种的蛋白质含量受种植密度的影响小于美国品种。蛋脂总含量受种植密度的影响较小，并且不存在品种间的差异性。磷酸二铵的施用量与大豆籽粒脂肪含量和蛋白质含量之间没有显著的相关性，但是中美品种的品质对磷酸二铵施用量的反应不同。磷酸二铵的施用量与中国品种的脂肪含量、蛋白质含量和蛋脂总含量呈正相关，而与美国品种的脂肪、蛋白质含量和蛋脂总含量呈负相关，但相关程度均不显著。 相似文献
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The variation in the kidding size of Black Bengal and Sirohi breed of goats makes them an interesting genetic material to study the underlying genetic mechanism of prolificacy. Accordingly, we studied the comparative ovarian morphometry including disparity in numbers of antral follicles of different sizes between these two breeds. Further, we evaluated the differential expression of the important candidate genes (viz., BMP15, GDF9 and BMPR1B) known to influence the ovulation rates and the prolificacy. The ovaries of Black Bengal (n = 20) goat were lighter (p < 0.01) in weight and smaller (p < 0.01) in diameter than those of Sirohi (n = 19) goats but possessed more numbers (p < 0.05) of corpus luteum (CL), large and small antral follicles. Quantitative real‐time PCR (RT‐qPCR) analysis revealed differential expression of mRNAs encoding for the BMP15 and GDF9. Small antral follicles of Black Bengal goats expressed 2.78‐fold more (p < 0.05) of BMP 15 than those of Sirohi goat. Expression of BMP15 (p < 0.01) and GDF9 (p < 0.05) mRNAs was more abundant in the small than the large antral follicles of Black Bengal goat. The more numbers of antral follicles per unit of ovarian mass and differential expression of BMP15 and GDF9 may serve as an important clue for higher prolificacy. 相似文献
73.
N Srivastava SK Srivastava SK Ghosh A Jerome GK Das S Mehrotra 《Reproduction in domestic animals》2013,48(5):724-731
PDC‐109, one of the most abundant proteins in bovine seminal plasma, has detrimental effect on spermatozoa in a time‐ and concentration‐dependent manner. Therefore, we hypothesized that sequestration of detrimental protein from ejaculates would be beneficial following cryopreservation of sperm cells. To this aim, we evaluated the effect of sequestration of PDC‐109 either by anti‐PDC‐109 antibodies (Ab) or egg yolk (EY) alone or by the synergistic action of EY + Ab in minimizing cryoinjury to bull spermatozoa. PDC‐109 protein was purified by applying two‐step chromatography procedures. The purified protein was injected in rabbits to raise antibodies which were isolated using ion‐exchange chromatography. After checking the Ab cross‐reactivity, they were quantitated and added to ejaculates, either alone or in addition to EY in Tris‐glycerol (TG) extender. Thus, ejaculates were processed in extender containing EY + TG (group I), Ab + TG (group II) or EY + Ab + TG (group III). Semen quality parameters (SQPs) viz. viability and acrosome integrity (FITC‐PSA), cryoinjury to spermatozoa (chlortetracycline, CTC assay) and in vitro fertility of protein‐sequestered‐semen (zona‐penetration assay) were evaluated. A significant (p < 0.05) improvement in post‐thaw SQPs as well as in non‐capacitated spermatozoa observed at pre‐freeze and post‐thaw stages of cryopreservation in group III compared with other groups indicated reduction in protein‐mediated cryoinjury. From this study, it can be concluded that sequestration of PDC‐109 by synergistic action of EY+Ab as compared to either of them alone significantly improve sperm quality and minimize cryoinjury to bull spermatozoa upon storage at ultra‐low temperatures. 相似文献
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