基于PLC的自动控制系统通讯过程的实现     

徐明娜  张峰  钱珊珠  贾翠玲  寇志伟 《内蒙古农业大学学报(自然科学版)》2013,(5):137-140

本文以OMRON CP1H可编程控制器为核心器件,介绍了该器件与LabVIEW以及变频器的通讯实现方法。通过试验表明,采用串行通讯方法来实现太阳能干燥控制是可行的,控制器能够实时显示系统温度变化情况。  相似文献   

啤酒大麦新品种垦啤6号的选育与高产栽培技术     

毋玲玲  何庆祥  张想平  钱永康  苏毓杰  李润喜 《种子》2011,30(1)

垦啤6号是以98-003为母本,以育种中间株系垦啤91134为父本进行有性杂交选育而成的优良啤酒大麦新品种。该品种麦芽品质优良、丰产稳产、高抗倒伏、耐密植、适应性广,平均产量8 967.0 kg/hm2,较对照甘啤4号增产13.2%;籽粒蛋白质含量10.5%,品质优级,是目前甘肃啤酒大麦产区理想的首选品种之一。2009年通过甘肃省非主要农作物品种认定(甘认麦2010003),并申请了国家农业部植物新品种保护(公告号:CNA 005816 E)。  相似文献   

黑龙江省乳业集群发展问题与对策研究     

李翠霞  钱薇 《渔业经济研究》2010,(6):29-33

黑龙江省乳业经历了快速的发展过程，乳业发展呈现集群发展的态势，但发展中也存在着集群区域品牌效应不强、专业化分工不明显、集群进入壁垒和退出壁垒高、奶牛养殖业饲养方式落后、集群内乳品企业重复建设、原料奶收购价格形成机制不合理和法律法规体系不健全等诸多问题。本文针对黑龙江省乳业集群发展中的问题，提出了解决的思路和对策。  相似文献   

猪Ghrelin基因的克隆及原核表达   总被引：1，自引：0，他引：1  

杨连玉  杨文艳  赵颖彩  钱剑  王哲 《中国兽医学报》2005,25(6):614-616

从猪下丘脑、胃等组织中提取总RNA，根据已发表的猪的Ghrelin mRNA序列设计合成引物，通过RT-PCR进行cDNA扩增，获得了282bp的片段。将该片段克隆于pMD-18T载体后进行序列分析，确认PCR产物为Ghrelin cDNA。从阳性克隆中提取质粒，经NheⅠ和XhoⅠ双酶切，回收282bp的目的片段，定向克隆到pET-28a表达载体中，提取质粒并再次转化到BL21（DE3）中，成功地筛选出阳性克隆。经IPTG诱导阳性菌，通过SDS-PAGE检测出猪Ghrelin基因的表达．  相似文献   

Effects of SOCS3 gene on the expression of c-myc mRNA and proliferation of rat pulmonary arterial smooth muscle cells under hypoxia conditions     

BAI Li  YU Zu-bin  QIAN Pin  QIAN Gui-sheng  GUAN Song 《园艺学报》2005,21(12):2310-2313

AIM: To explore the effect of SOCS3 gene on the expression of c-myc mRNA and proliferation of rat pulmonary arterial smooth muscle cells (PASMCs) under hypoxia conditions. METHODS: PASMCs was cotransfected with pEFSOCS3 and pSV2neo by lipofectamine, and positive cell clones were obtained after being screened with G418. Expressions of SOCS3 protein in PASMCs before and after transfection were detected by immunocytochemistry, respectively. Before and after transfection, PASMCs were exposed to normoxic and hypoxia conditions at various time points, respectively, and the expressions of c-myc mRNA were assessed by semi-quantitive RT-PCR. ［3H］-TdR incorporation method was used to detect the cell proliferation. RESULTS: The expression of SOCS3 protein was confirmed by immunocytochemistry in PASMCs transfected with SOCS3 gene. c-myc mRNA level in the SOCS3 gene-transfected cells exposed to hypoxia were remarkablely lower than that in the control cells, respectively (P<0.01). Compared with the control groups at the same time points, ［3H］-TdR incorporation in SOCS3 gene-transfected cells was significantly low. CONCLUSION: SOCS3 protein may inhibit the proliferation of PASMCs by downregulating the c-myc gene expression under hypoxia conditions.  相似文献   

樱桃谷鸭育雏关键技术措施     

刘渊  钱续 《中国动物保健》2005,(2):47-48

雏鸭是指刚孵出至15日龄的鸭,是鸭一生中增重最快、饲料报酬最高的时期,但由于它对外界环境适应能力差,对疾病的抵抗力低,因而又是最难饲养的时期。所以雏鸭饲养是培育种鸭和养好商品鸭最为重要的阶段。这一阶段饲养管理得好可以收到事半功倍的效果,否则不但育雏成活率低,而且将严重影响日后生长发育,增大饲养成本,降低养鸭的效益。作者在完成兰州市科委下达的“樱桃谷鸭引种推广养殖试验”科研课题中,对如何做好雏鸭饲养作了认真的研究探讨,在三年多的推广养殖中育雏成活率保持在90%～100%,现将关键技术措施总结如下,供参考。一、育雏前的…  相似文献   

西南地区农田主要种植草种及种植模式探讨     

朱栋斌  罗富成  文际坤  钱庆 《草原与草坪》2006,(3):17-19

利用南方农区丰富的农田资源,大力发展草地畜牧业,引草入田,是发挥草业优势,突破农村发展瓶颈,增加农民收入的一项重要措施。探讨了西南地区农田的主要种植草种以及适宜西南地区的种植利用模式。  相似文献   

鸽源冠状病毒PSH株纤突蛋白基因的遗传变异分析     

钱华冬吴祖立  华修国朱建国 《中国兽医科技》2006,36(3):199-202

对鸽源冠状病毒PSH株纤突蛋白基因进行了RT—PCR扩增、克隆和测序。结果表明，PSH株S基因由3504个核苷酸组成，编码1条由1167个氨基酸残基组成的多肽。S基因编码产物裂解后形成的S1和S2亚单位分别由541和626个氨基酸残基组成。PSH株S蛋白切割识别位点为精氨酸-精氨酸-苯丙氨酸-精氨酸-精氨酸（RRFRR），与多数鸡传染性支气管炎病毒（IBV）切割识别位点相似（RRF／SRR）。与GenBank中其他冠状病毒毒株S基因的推导氨基酸序列相比较，PSH株与IBV参考毒株的同源性为79．3％～99．6％，而与其他冠状病毒包括火鸡蓝冠病病毒和SARS病毒的同源性均小于37．8％。表明，鸽源冠状病毒PSH株属于第3抗原群冠状病毒，且与IBV亲缘关系较近。  相似文献   

Isolation,Identification and Biological Characteristics of Staphylococcus chromogenes from Tibetan Pigs     

ZHAO Xialing  XIAO Qi  QIAN Wenxian  WEN Dongxu  YAN Mingshuai  LYU Lixin  NIU Jiaqiang  HE Kongwang 《中国畜牧兽医》2007,47(9):3014-3021

In order to understand the infection of Staphylococcus disease in healthy Tibetan pigs in Linzhi city,Tibet.232 samples were collected from the slaughterhouse in Bahe town,Gongbujiangda county,Linzhi city,Tibet,the teaching practice ranch at the Tibet Agriculture and Animal Husbandry University in Bayi district,Linzhi city,and the slaughterhouse in Bayi district,Linzhi city.These samples were isolated,cultured and identified,and one isolated strain was systematically studied for biological characteristics.Epidemiological survey results showed that 73 of 232 samples were positive for Staphylococcus,the positive rate was 31.47%,16 positive samples were detected in the slaughterhouse of Bahe town,Gongbujiangda county,Linzhi,Tibet,with a detection rate of 23.89%(16/67),43 positive samples were detected in the pastures of the teaching practice ranch at the Tibet Agriculture and Animal Husbandry University in Bayi district,Linzhi city,with a detection rate of 34.40% (43/125),14 positive samples were detected in the slanghterhouse in Bayi district,Linzhi city,with a detection rate of 35.00% (14/40).Through PCR identification,gene sequencing and biochemical identification,5 strains of Staphylococcus chromogenes were isolated,and one of them (Sa LZ1) was studied systematically.The biological characteristics of the isolated strains showed that the Sa LZ1 strain formed round white convex colonies on the agar medium,with regular edges,smooth surfaces,and opaque white colonies.The isolates were Gram-positive cocci bacteria.PCR sequencing results showed that the isolate had the highest homology with Staphylococcus chromogenes (GenBank accession No.:MG576253.1),positive for serum inulin,manicol,glucose,saccharose,lactose,negative for arabinose,sodium hippurate,raffinose,xylose,urea,etc,consistent with the biochemical characteristics of Staphylococcus chromogenes.The logarithmic growth period of isolated bacteria was 5 to 10 h.The pathogenicity test of mice showed that the mortality rate of mice reached 100% when the concentration of Sa LZ1 strain reached 3.5×10⁹ CFU/mL.And the results of pathological section showed that Sa LZ1 strain could cause the visceral tissue of mice to develop pathological changes.The above results provided a theoretical basis for the prevention and control of Staphylococcus suis in Linzhi,Tibet.  相似文献   

药食观赏兼用特菜菊花脑无公害栽培技术   总被引：2，自引：0，他引：2  

马国胜  王晖  钱兰华 《北方园艺》2006,(2):72-73

菊花脑属菊科菊属多年生宿根性草本植物,在江苏多为田边地头野生,近年来有小面积零星栽培,10～11月开花,人们常在4～8月用手掐取其嫩头作为蔬菜食用,具有清热解毒、凉血降压之功效,是一种栽培简易的具有开发价值的营养保健型蔬菜,兼有药用、食用和观赏三重价值.  相似文献