Studies on efficacy and stability of a vaccine bait containing ERA strain of rabies virus propagated in a BHK-21 cell line.          下载免费PDF全文

K F Lawson  H Chiu  M Matson  P Bachmann    J B Campbell 《Canadian journal of veterinary research》1992,56(2):135-141

In a dose response study in foxes, the median protective dose of ERA BHK21 vaccine in a blister pack bait was 10(6.0) tissue culture infective doses (TCID)/mL, while artificially aged baits with titers of 10(6.3) TCID/mL induced seroconversion in 78% of foxes. There was no significant difference in the development of antibodies in foxes receiving 1, 2 or 3 mL volumes of vaccine in the bait. When baits were exposed to the elements and fed to foxes over a 21 day period, 85% of the animals seroconverted. Age, sex and the way in which the vaccine container was contacted did not appear to be factors in the responses of these animals. Juvenile foxes, approximately six months of age, were marked more readily with the tetracycline bait marker than older animals. Approximately 25% of foxes did not appear to respond well to vaccination and the titer of the vaccine was a critical factor in producing seroconversion in these animals.  相似文献   

Inheritance of myotonic discharges in American quarter horses and the relationship to hyperkalemic periodic paralysis.          下载免费PDF全文

J M Naylor  J A Robinson  E C Crichlow    J E Steiss 《Canadian journal of veterinary research》1992,56(1):62-66

Electromyography (EMG) was used to detect myotonic discharges in Quarter Horse breeding stock and to follow the results of mating horses with hyperkalemic periodic paralysis (HPP). The studies were performed on two brood mare farms. A total of six breeding stock showed myotonic discharges and 15 were nonmyotonic. Myotonic discharges were seen in five of six horses belonging to the blood line previously implicated as being predisposed to HPP. Two of these horses had shown clinical signs of HPP. Only one of 15 breeding horses unrelated to the HPP predisposed blood line showed myotonic discharges. When both parents were non-myotonic on EMG than the F1 generation (n = 6) were also nonmyotonic. When a stallion with HPP and myotonic discharges was mated to eight nonmyotonic mares over a six year period half the animals of the F1 generation (n = 25) showed myotonic discharges. When both parents showed myotonic discharges four F1 offspring were myotonic and two were nonmyotonic on EMG testing. There was no evidence of sex linkage. The results are consistent with an autosomal dominant mode of inheritance. Hyperkalemic periodic paralysis and myotonic discharges on EMG may be different manifestations of the same underlying defect.  相似文献   

Obituary: Dr. William J. Dorward          下载免费PDF全文

W.G.D. Yates  Peter Ide 《The Canadian veterinary journal. La revue veterinaire canadienne》1992,33(8):492

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Studies on mutlispecific resistance of gastrointestinal nematodes to benzimidazoles on dairy-goat farms     

J. Cabaret  H. M. Baudet  J. Devos  J. Hubert  J. Cortet  C. Sauv 《Veterinary parasitology》1995,60(3-4):331-337

Multispecific resistance to benzimidazoles was studied in three selected farms. These farms had bred dairy goats for more than 15 years. The helminths were introduced with the goats at the establishment of the farms which afterwards remained isolated. Nematode resistance could then be related to their own management practices. Faecal egg count tests and egg hatch assays were performed to assess intensity of resistance. The generic (infective larvae in faecal cultures) and specific richness (adult worms) were assessed. The resistant species were Trichostrongylus colubriformis, Teladorsagia circumcincta, Haemonchus contortus and Oesophagostomum venulosum. Faecal egg count reduction tests and egg-hatch assays did not match exactly. Faecal larval counts after treatments gave a distorted picture of multispecific resistance: Haemonchus and Oesophagostomum were very largely over represented. The number of species found in the three farms was relatively low compared with other reports in goat farms of the area. This reduction of diversity might also be due in part to characteristics of breeding management and history (use of permanent pasture and introduction of goats at the establishment of farm).  相似文献   

不确定度评定方法在汽车事故再现中的应用     

邓海英莫耀祖  蒋淑霞韩志刚 《中南林学院学报》2006,26(4):96-100

介绍了不确定度理论及其评定方法，结合车辆碰撞事故的具体特点，给出了基于能量守恒的车速计算表达式．通过选择适当的输入参数作为不确定因子，分别计算各分量等效速度标准不确定度．考虑不确定度累积和传播，得到车速输出结果的合成标准不确定度和展伸不确定度，以此检验事故再现结果的准确程度．通过具体事故案例的分析计算和计算机检验，验证该方法的适用性．  相似文献   

新的β-二氢沉香呋喃倍半萜多元酯和生物碱的HR-FABMS研究     

刘伟  姬志勤  张启东  吴文君  王明安 《西北农业学报》2006,15(6):225-228

利用高分辨快原子轰击质谱(HR-FABMS),以(甘油 氯化钠)为基质测定了9个新的β-二氢沉香呋喃倍半萜多酯和生物碱的HR-FABMS。结果表明,所有该类化合物均可以精确地获得[M Na] ,[M H] ,[M H-H2O] 等特征离子的质量和元素组成,从而方便地获得原有该类化合物的分子量和分子式。  相似文献   

Verification by polymerase chain reaction of vertical transmission of Theileria sergenti in cows   总被引：2，自引：0，他引：2       下载免费PDF全文

Byeong K. Baek  Kim B. Soo  Jin H. Kim  Jin Hur  Bou O. Lee  J. M. Jung  Misao Onuma  Anthony O. Oluoch  Chang-Hyun Kim    Ibulaimu Kakoma 《Canadian journal of veterinary research》2003,67(4):278-282

To evaluate the transplacental transfer of Theileria sergenti infection in cattle, we used DNA probes to detect T. sergenti in 6 pregnant cows and their calves. All the animals were monitored by parasitologic, serologic, and polymerase chain reaction (PCR) assays for a predicted 875-base-pair (bp) DNA product and a 684-bp amplicon detected by nested PCR in the blood and spleens of aborted fetuses. An open reading frame (ORF) starting at nucleotide 170 and terminating at position 1021 was shown to code for a polypeptide of 283 amino acid residues. All 6 dams and 5 calves were positive for T. sergenti in all tests. One calf was positive only with nested PCR. We conclude that transplacental transmission of T. sergenti is a significant problem. The relevance of the data in the programmed introduction of new (especially pregnant) animals into established clean herds needs serious consideration with regard to control of theileriosis and other tickborne diseases.  相似文献   

柔嫩艾美耳球虫感染鸡盲肠和脾脏一氧化氮合酶的表达   总被引：2，自引：0，他引：2  

李金贵  朱蓓蕾  蒋金书 《中国兽医学报》2003,23(4):341-343

采用NADPH—d(nicotinamide adenine dinucleotide phosphate—diaphroase)组织化学法观察了雏鸡感染柔嫩艾美耳珠虫(E．tenella)后一氧化氮合酶(N0S)在盲肠和脾脏中的分布与表达情况。试验结果表明，所有正常鸡盲肠粘膜下层和肌层均有较深的着色，根据以往的资料和N0S的表达特性初步判断为神经元型N0S(nNOS)；试验鸡在感染后3～5d，盲肠粘膜上皮和肠腺上皮也有较深的着色，并从感染后7d开始着色减弱；而对照组鸡盲肠粘膜上皮和肠腺上皮以及对照组和试验组的脾脏几乎不着色或着色很浅。试验结果提示，盲肠粘膜上皮和肠腺上皮的着色可能是诱导型N0S(iN0S)表达的结果，而由其产生的N0参与雏鸡球虫感染过程。  相似文献   

The use of a Giardia vaccine as an immunotherapeutic agent in dogs.          下载免费PDF全文

M E Olson  C J Hannigan  P F Gaviller    L A Fulton 《The Canadian veterinary journal. La revue veterinaire canadienne》2001,42(11):865-868

Dogs (n = 13), which had failed to be cured of giardiosis following chemotherapeutic measures, were treated with a Giardia vaccine (2-3 injections). Clinical signs resolved between 16 and 42 days postvaccination and cessation of fecal cyst shedding was between 21 and 70 days. Vaccination is a potential method of treating giardiosis in dogs.  相似文献   

河北省天然草地禾本科植物的生活型、生态型及区系分析     

任永霞  郭郁频  吕进英  王海  姜风琴 《草业科学》2001,18(1):1-3

对河北省天然草地禾本科植物的生活型、生态型及区系特征进行了分析，结果表明，多年生草本为优势生活型，占78．4％；生态型以中生植物为主，占77．2％，旱生植物占16．0％，湿生植物仅占6．8％；植物区系成分多样，温带分布属最多达42属，占总属数的57．5％，其次为热带分布属，有24属，占32．9％。  相似文献